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All Journal The Journal of Experimental Life Sciences (JELS) Jurnal Ilmiah Biosaintropis (Bioscience-Tropic) JIMR - Journal of Islamic Medicine Research Jurnal Kesehatan Islam : Islamic Health Journal Jurnal Pendidikan Kedokteran Indonesia: The Indonesian Journal of Medical Education Borneo Journal Of Pharmascientech Scientific Journal Jurnal Kedokteran Komunitas (Journal of Community Medicine) International Journal of Health, Engineering and Technology Borneo Journal of Pharmacy
Risandiansyah, Rio
Faculty Of Medicine, Universitas Islam Malang
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Dentistry Education Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Mechanical Engineering Medicine & Pharmacology Neuroscience Nursing Public Health Veterinary

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The Use of Food Coloring Dyes in Bacterial Staining Risandiansyah, Rio; Arniyati, Arniyati; Nurita, Nofie Irmalia; Gionika, Natasya Hana
The Journal of Experimental Life Science Vol. 10 No. 2 (2020)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2020.010.02.10

Abstract

Staining creates a contrast between the cells and its surrounding, and enables the microscopic characteristics of bacterial cells to be easily visible and distinguished. However, staining often relies on dyes which are expensive, not readily available, or toxic. In this study, the use of food coloring dyes to stain bacteria was explored. We stained Gram- positive bacteria (Bacillus sp. and Staphylococcus aureus) and Gram-negative bacteria (Escherichia coli) using several food coloring dyes of different colors, which were purchased locally. After slide fixation, the dye was flooded on the bacterial smear and air-dried for up to 30 minutes and observed by using microscope before and after washing with water. The results of this study show that prior to washing, most food coloring dyes were able to stain bacterial cells. However, after washing, only pink and purple food coloring dyes were retained, showing pink colored cells. We suspected that erythrosine was the agent responsible for this result, and was able to show similar characteristics with erythrosine alone. This study concludes that food coloring dyes containing erythrosine can be used to stain bacterial cells indiscriminately.Keywords: Bacterial cell staining, erythrosine, food coloring dyes, resource-limited.
Interaksi Antara Ekstrak Clitoria ternatea L Dan Antibiotik Pada Aktivitas Antibakteri Terhadap Staphylococcus aureus Nabila Wanda, Yumna Hanum; Rahma Putri, Citra Destya; Risandiansyah, Rio
BORNEO JOURNAL OF PHARMASCIENTECH Vol 10 No 1 (2026): Borneo Journal of Pharmascientech
Publisher : Universitas Borneo Lestari

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59053/bjp.v10i1.800

Abstract

Antibiotic combination is a strategy to enhance antibacterial effectiveness by acting on different cellular targets. The utilization of natural ingredients as adjuvants also has the potential to improve antimicrobial responses. Butterfly pea (Clitoria ternatea L.) is known to contain antibacterial compounds that can potentiate antibiotic activity. Research regarding the interaction of Clitoria ternatea extracts with antibiotics against Staphylococcus aureus remains limited; therefore, this study aims to evaluate the interaction patterns of these combinations. Clitoria ternatea L. simplicia was macerated using three solvents: ethanol, ethyl acetate, and n-hexane. Antibacterial testing was conducted based on the Zone of Inhibition (ZOI) using the Ameri-Ziaei Double Antibiotic Synergism Test (AZDAST) method, which is suitable as an initial screening method for antimicrobial interactions. Extracts were tested at a concentration of 50% (500,000 ppm), with the inoculum standardized to 0.5 McFarland, and each treatment was replicated 3×2 times. Data were analyzed using One-way ANOVA or non-parametric tests (Kruskal-Wallis) depending on the data distribution, followed by appropriate post hoc tests (Tukey, Games-Howell, or Mann-Whitney). The significance level was set at p < 0,05, and the type of interaction was determined based on AZDAST interpretation criteria (synergistic, additive, antagonistic, or not distinguishable). The results showed that the combination of ethanol extract and amoxicillin produced an additive effect (55.06 ± 1.68 mm), The combination of ethyl acetate extract with amoxicillin (52.38 ± 2.60 mm). n-hexane extract with amoxicillin (52.60 ± 3.99 mm), and all extract combinations with tetracycline (37.76-42.70 mm) yielded results that were not distinguishable compared to either single antibiotics or their double doses. It can be concluded that the combination of ethanol and ethyl acetate extracts with amoxicillin can enhance antibacterial activity compared to single treatments by providing an additive effect. Meanwhile, the combinations of ethanol and ethyl acetate extracts with tetracycline, as well as the combinations of n-hexane extract with both amoxicillin and tetracycline, showed no significant difference (not distinguishable).
Co-Authors alma nur anisa, rizki Annur Affin Niswah Arif Yahya Arlisyah, Annisa Arniyati, Arniyati Brillian Nur Muhammad Cahya Dhimas Triatmojo Putra Difa Rizqi Akmalia Durrotun Ni'mah EKO HARI PRASETYO Evilya Fitra Indriana Faiqo Nabila Faris Akbar Maulana Ghazi Ghazi Gionika, Natasya Hana Hakim, Reza Hardadi Airlangga Hardadi Airlangga Hardadi Airlangga Helmin Elyani Heru, Arief Ibrahim Ashri Iqbal Ismail Mochsen Isna Aulia Zamzamy Khalfia Abdan Firani Kurniawati, Annisa Marindra Firmansyah Maulani , Maghfirah Nur Muhamad Hudaya Setio Utari Muhammad Sandy Ali Yafie Muhammad Zainul Fadli Nabila Wanda, Yumna Hanum Nina Oktavia Noer Aini Nova Faisal Waber Nurita, Nofie Irmalia Nurma Alifia Rakhma Primadita, Hanggia Putri Lissanawidya Putri, Amanita Dias Ezha Rahma Putri, Citra Destya Reza Hakim Riza Ma’rufin Riza Ma’rufin Rizky Fajar Imam Asshiddiq Sasi Purwanti Shabrina Yasyfi Hanifati Shafira , Iftah Ghina Syarieb, Ahmad Akbar Wahyuni Dwi Permata Sari Wicaksono, Arief Heru Wika Yuli Deakandi Yahya, Arif Yoni Rina Bintari Yulia Resty