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All Journal Jurnal Kesehatan Andalas Molecular and Cellular Biomedical Sciences (MCBS) Jurnal Sains dan Teknologi: Jurnal Keilmuan dan Aplikasi Teknologi Industri Majalah Kedokteran Andalas Bioscientia Medicina : Journal of Biomedicine and Translational Research Health and Medical Journal Bioscientia Medicina : Journal of Biomedicine and Translational Research Indonesian Journal of Multidisciplinary Science Jurnal Ilmu Kesehatan Indonesia (JIKSI) The Hip and Knee Journal
Rizki Rahmadian
Bagian Bedah, Fakultas Kedokteran, Universitas Andalas, Kampus Unand Limau Manis Pauh, Padang, Sumatera Barat, Indonesia
Humanities Biochemistry, Genetics & Molecular Biology Computer Science & IT Decision Sciences, Operations Research & Management Dentistry Economics, Econometrics & Finance Education Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Library & Information Science Mathematics Medicine & Pharmacology Neuroscience Public Health Social Sciences

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Comparison of the Effectiveness of Lyophilized Amniotic Membranes in the Partial Thickness Wound Healing Process in Mus musculus Srigunda Arisya Fadilla; Rizki Rahmadian; Benni Raymond
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 7 No. 11 (2023): Bioscientia Medicina: Journal of Biomedicine & Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37275/bsm.v7i11.887

Abstract

Background: Currently, tulle or gentamicin ointment is generally used to prevent wound infections in wound care. Human amniotic membrane has been used in medical fields such as wound care. So far, there is no comparative evidence of the effectiveness of each ingredient. This study aimed to compare the effectiveness of lyophilized amniotic membrane, tulle, and 0.1% gentamicin ointment in the wound healing process. partial thickness on Mus musculus clinically and histologically. Methods: This type of research is experimental with a post-test-only control group design. There were 24 rats divided into each group. This research was conducted at the anatomical pathology laboratory, Faculty of Medicine, Universitas Andalas, and INA lab in July - September 2023 using rats (Mus musculus), which were divided into 3 treatment groups and one control group. Results: Clinically, lyophilized amniotic membrane, 0.1% gentamicin ointment, and tulle provided better results than controls regarding erythema variables. Histologically, the results showed that the lyophilized amniotic membrane group had the thinnest epidermis and dermis, as well as better histological scores for granulation, fibroblasts, and collagen than the 0.1% gentamicin ointment, tulle, and control groups. In this study, significant differences in effectiveness were found in terms of erythema, skin thickness, granulation, fibroblast, and collagen histological scores, with better results in the lyophilized amniotic membrane group. Conclusion: Clinically, lyophilized amniotic membrane, tulle, and 0.1% gentamicin ointment were more effective than controls for wound treatment due to faster reduction in erythema. However, histologically, the lyophilized amniotic membrane was proven to be more effective than tulle, 0.1% gentamicin ointment, and control in terms of epidermis and dermis thickness, granulation level, fibroblasts, and collagen.
Comparison of the Effectiveness of Lyophilized Amniotic Membranes in the Partial Thickness Wound Healing Process in Mus musculus Fadilla, Srigunda Arisya; Rizki Rahmadian; Benni Raymond
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 7 No. 11 (2023): Bioscientia Medicina: Journal of Biomedicine & Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37275/bsm.v7i11.887

Abstract

Background: Currently, tulle or gentamicin ointment is generally used to prevent wound infections in wound care. Human amniotic membrane has been used in medical fields such as wound care. So far, there is no comparative evidence of the effectiveness of each ingredient. This study aimed to compare the effectiveness of lyophilized amniotic membrane, tulle, and 0.1% gentamicin ointment in the wound healing process. partial thickness on Mus musculus clinically and histologically. Methods: This type of research is experimental with a post-test-only control group design. There were 24 rats divided into each group. This research was conducted at the anatomical pathology laboratory, Faculty of Medicine, Universitas Andalas, and INA lab in July - September 2023 using rats (Mus musculus), which were divided into 3 treatment groups and one control group. Results: Clinically, lyophilized amniotic membrane, 0.1% gentamicin ointment, and tulle provided better results than controls regarding erythema variables. Histologically, the results showed that the lyophilized amniotic membrane group had the thinnest epidermis and dermis, as well as better histological scores for granulation, fibroblasts, and collagen than the 0.1% gentamicin ointment, tulle, and control groups. In this study, significant differences in effectiveness were found in terms of erythema, skin thickness, granulation, fibroblast, and collagen histological scores, with better results in the lyophilized amniotic membrane group. Conclusion: Clinically, lyophilized amniotic membrane, tulle, and 0.1% gentamicin ointment were more effective than controls for wound treatment due to faster reduction in erythema. However, histologically, the lyophilized amniotic membrane was proven to be more effective than tulle, 0.1% gentamicin ointment, and control in terms of epidermis and dermis thickness, granulation level, fibroblasts, and collagen.
Efek Pemberian Ekstrak Daun Sungkai (Peronema canescens Jack) Terhadap Proliferasi dan Migrasi Sel Kanker Serviks HeLa Meilisa Rahmawati; Dessy Arisanty; Rizki Rahmadian; Eti Yerizel; Dina Arfiani Rusjdi; Rahmatini Rahmatini
Majalah Kedokteran Andalas Vol 46, No 10 (2024): Supplementary April 2024
Publisher : Faculty of Medicine, Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/mka.v46.i10.p1545-1561.2024

Abstract

Tujuan: mengetahui pengaruh pemberian ekstrak daun sungkai dalam menghambat proliferasi dan migrasi sel HeLa.Metode: Penelitian ini merupakan penelitian eksperimental yang menggunakan 24 disk kultur sel HeLa yang konfluens. Sampel dibagi menjadi 4 kelompok (K,P1,P2 dan P3). Kelompok perlakuan diberikan ekstrak daun sungkai dengan konsentrasi IC25, IC50, dan IC75 yang diinkubasi selama 72 jam. Analisis data menggunakan uji One Way ANOVA dan Post Hoc Bonferroni. Hasil: Rerata jumlah sel proliferatif yang didapatkan pada kelompok K, P1, P2, dan P3 berturut-turut adalah 12,1 x 105, 8,94 x 105, 6,14 x 105 dan 5,04 x 105. Rerata migrasi jumlah sel yang berkumpul pada zona scratch pada kelompok K, P1, P2, dan P3 berturut-turut adalah 49,.0, 237,8, 210,5, dan 100,7. Didapatkan perbedaan yang bermakna tiap kelompok pada uji proliferasi dan migrasi dengan nilai p = 0,000 (p < 0,05).Kesimpulan: Terdapat pengaruh pemberian ekstrak daun sungkai terhadap proliferasi dan migrasi sel HeLa. Perbedaan bermakna ditemukan pada kelompok kontrol dan kelompok perlakuan dengan konsentrasi ekstrak IC25, IC50, dan IC75.
Co-Authors Amir, Arni Amirah Zatil Izzah Andani Eka Putra Arafat, Andril Armenia, Armenia Armia Indra Nur Alam Asep Santoso Benni Raymond Benni Raymond Benni Raymond Benni Raymond Cokorda Gde Oka Dharmayuda Dafit Firmansyah Dessy Arisanty Dia Rofinda, Zelly Dia Dicky Mulyadi Dina Arfiani Rusjdi Dwi Yulia Ebill Fuji Edison Erkadius Erkadius Eti Yerizel Fadilla, Srigunda Arisya Farah Sevnell Yanti Firdawati Firdawati Fitri Amita Hafni Marsil Hanna Sari Widya Kusuma Hanum, Fathiya Juwita Hendra Maska Hermansyah Hermansyah Hirowati Ali Ika Adhani Sholihah Kaharudin Kaharudin Khalisha Azzahra Pramesti Abdillah Krisna Yuarno Pratama M. Nasyarudin Latif Marlina . Meilisa Rahmawati Miftah Irrahmah Muhamad Ivan Muhammad Andry Usman Muhammad Ihsan Nanda, Is Prima Nasman Puar Nur Sadia Binti Suhail Ahmad Rahmatini . Raniathia Frieska Reza Rahman Ramadhani Rizal Rizal Rizanda Machmud Roni Eka Sahputra Rudy Afriant Satrio Haryo Benowo Wibowo Selfi Renita Rusjdi Seprianto Seprianto Sharon Serafim Bosawer Sholahuddin Rhatomy Siregar, Chairiandi Siti Nurhajjah Srigunda Arisya Fadilla Syahril Syahril Sylvia Rachman Taufik Rachman Tofrizal Tofrizal Vivi Sofia Wahyu Setia Widodo Wahyu Widowati Wulandari Taradita Yona Riapesi