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All Journal Jurnal Gizi dan Pangan JURNAL GIZI INDONESIA Jurnal Kedokteran Diponegoro Journal of Nutrition College Media Gizi Mikro Indonesia Preventif: Jurnal Kesehatan Masyarakat Jurnal Gizi Klinik Indonesia Amerta Nutrition Preventif : Jurnal Kesehatan Masyarakat
Binar Panunggal
Departemen Ilmu Gizi, Fakultas Kedokteran Universitas Diponegoro, Semarang
Aerospace Engineering Biochemistry, Genetics & Molecular Biology Environmental Science Health Professions Medicine & Pharmacology Nursing Public Health Other

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Journal : Amerta Nutrition

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Total Lactic Acid Bacteria and Antibacterial Activity in Yoghurt with Addition of Ananas comosus Merr. and Cinnamomum burmannii Lusia Yotista Enggal Parasthi; Diana Nur Afifah; Choirun Nissa; Binar Panunggal
Amerta Nutrition Vol. 4 No. 4 (2020): AMERTA NUTRITION
Publisher : Universitas Airlangga, Kampus C, Mulyorejo, Surabaya-60115, East Java, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/amnt.v4i4.2020.257-264

Abstract

Background: Diarrhea disease is one of gastrointestinal disorders which is the second leading cause of death in children under five years. Food and beverage contamination is the biggest cause of diarrhea in developing countries. Nanas madu (Ananas comosus Merr.) and cinnamon (Cinnamomum burmannii) have antibacterial properties that can inhibit the growth of Escherichia coli and Salmonella typhi causing diarrhea.Objective: This study aimed to analyze the total differences of Lactic Acid Bacteria (LAB) and antibacterial activity in yoghurt with the addition of nanas madu and cinnamon extract.Methods: This was an experimental study with various treatment in adding honey cream pineapple (0%, 20%, 40%, and 60%) and cinnamon extract (4% and 6%). Total LAB was calculated using the Total Plate Count (TPC) method and antibacterial activity was tested using Kirby Bauer method.Results: There was no significant difference in yoghurt with the addition of honey cream pineapple and cinnamon extract. Yoghurt with the highest LAB was yoghurt with addition 40% of honey cream pineapple and 6% of cinnamon extract (N40M2) with total LAB 1,43 x 1019 CFU/ml. The results of the antibacterial activity showed no significant difference of inhibition zone against S. typhi¸ while there was significant difference of inhibition zone against E. coli. The highest activity against S. typhi was yoghurt with addition 60% of honey cream pineapple and 4% of cinnamon extract (N60M1) resulting 6,81 mm inhibition zone and the highest activity against E. coli was N40M1 resulting 6,77 mm of inhibition zone. Conclusion: Total LAB yoghurt with the addition of nanas madu and cinnamon extract have met FAO and SNI standards with LAB minimum 107 CFU/ml. Antibacterial activity of all yoghurt treatment categorized as medium inhibition (5-10 mm). 
Pengaruh Kefir Susu Kambing Terfortifikasi Vitamin D3 terhadap Kadar hs-CRP Tikus Rattus Norvegicus DM Tipe 2: Studi Eksperimental: The Effect of Vitamin D3-Fortified Goat Milk Kefir on hs-CRP Levels of Type 2 Diabetic Rattus Norvegicus Rats: An Experimental Study Zulfa, Fairuz; Utomo, Astika Widy; Ardiaria, Martha; Syauqy, Ahmad; Purwanti, Rachma; Ernalia, Yanti; Masha, Tania; Maharani, Mutiara Irma; Faradina, Amelia; Panunggal, Binar
Amerta Nutrition Vol. 9 No. 4 (2025): AMERTA NUTRITION (Bilingual Edition)
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/amnt.v9i4.2025.667-678

Abstract

Background: Insulin resistance and chronic hyperglycemia in diabetes are linked to the synthesis of the inflammatory biomarker hs-CRP. Goat milk kefir and vitamin D have anti-diabetic and anti-inflammatory properties. Objectives: Aimed to evaluate the impact of goat milk kefir fortified with vitamin D3 on hs-CRP levels of diabetic rats. Methods: Twenty-one male Rattus norvegicus rats were randomly divided into four groups for a 35-day study: Control (C), Diabetes Mellitus (DM), unfortified goat milk kefir treatment (P1), and vitamin D3-enriched goat milk kefir treatment (P2). Diabetes was induced via intraperitoneal injection of streptozotocin (STZ) at 65 mg/kg BW and nicotinamide (NA) at 230 mg/kg BW. Goat milk kefir was administered orally at 2 mL/200 g BW/day, with the fortified version containing 600 IU of vitamin D3 per 100 mL. Fasting blood glucose levels and serum hs-CRP were measured pre- and post-intervention using the GOD-PAP and ELISA methods, respectively. Data were analyzed using bivariate and multivariate methods in GraphPad Prism 8. Results: There was a statistically insignificant decrease in hs-CRP levels in the P2 group (p-value=0.21) and in the P1 group (p-value=0.63), suggesting limited impact on inflammation. However, there was a statistically significant drop in blood glucose levels in the P2 group (∆FBG -65.50±35.44 mg/dL, p-value=0.03) and in the P1 group (∆FBG -81.63±50.07 mg/dL, p-value=0.05). Conclusions: The reduction in hs-CRP levels indicates that vitamin D3-fortified kefir may help modulate low-grade inflammation and shows promise in managing diabetes. Future research should examine dose, duration, and sample size for better efficacy assessment.
Co-Authors Adriyan Pramono Ahmad Syauqy Anggraeni, Neni Anggraini, Wiriesta Anjani, Gemala Aryu Candra Asnelviana, Hana Astika Widy Utomo, Astika Widy Ayu Rahadiyanti Balgis Balgis Bintang, Fransisca Natalia Chairunnisa, Estillyta Choirun Nissa Choirun Nissa Dea Mustika Hapsari, Dea Mustika Deny Yudi Fitranti Dewi Marfu’ah Kurniawati Dewi Mulad Sari Dewi, Clara Rashinta Dewi, Listiyani Kusumo Diana Nur Afifah, Diana Nur Dita Adi Subiakti Dwi Arum Sulistyaningsih Emma Kurniawaty, Emma Enny Probosari Etika Ratna Noer Etisa Adi Murbawani Fachrana Fachrana Faradina, Amelia Febrian Saniarto Fillah Fithra Dieny Fitriyono Ayustaningwarno Gusria Yuana Haidar Asshidiqie Hartanti Sandi Wijayanti Henny, Via Anugrah Hertanto Wahyu Subagio Ignatius Roy Indra P, Ignatius Roy Iqlima Safitri, Iqlima Khikmaturrohman, Sya’bani Isnaen Khoiriyah, Dian Khoiriyah, Dian Kirana, Stela Maris Adinda Budi Lestari, Rahma Wati Dwi Linda Isdamayani, Linda Lusia Yotista Enggal Parasthi Maharani, Mutiara Irma Marsa, Firdaus Shadiqa MARTHA ARDIARIA Masha, Tania Maulana, Reza Achmad Mohammad Sulchan Mohammad Zen Rahfiludin Muhammad Sulchan Mumpuni, Ocka Febrian murbawani, etisa ardi Ningsih, Diajeng Dian Rahana Ningsih, Ryta Ristantia Nugrahani, Gardinia Nugraheni, Dini Nuris Zuraida Rakhmawati Nurkomala, Siti Nurmasari Widyastuti Nuryanto Nuryanto Pramono, Adryan Pratiwi, Hera Rachma Purwanti Rahayu, Gita Riski Rahma Wati Dwi Lestari Rahma, Fadilla Ratna Fathurrizqiah, Ratna Rejeki, Vina Puji Riani Arifin Rosmalinda, Yusie Setyawan, Fajar Siringoringo, Ester Theresia Siti Santiaji Pursriningsih, Siti Santiaji Sukmasari, Vintantiana Suryaningtyas, Ratih Tiara Aris Dahriani Tika Mei Indah Susanti, Tika Mei Indah Winarto, Zahra Qurrota A'yun Yanti Ernalia Yusuf Hidayat Zulfa, Fairuz