A high organizational culture can lead to conduciveness at work, related to employee comfort and order. Organizational culture plays an important role in improving education in Indonesia by influencing the attitudes and activities of educators and education personnel in the educational environment. The purpose of this study was to determine the effect of organizational culture (X) on employee performance (Y). The research paradigm is one of the researchers' references in formulating the research hypothesis. The results of organizational culture research at Bina Sarana Informatika University are in a very high category as measured by 5 dimensions, namely observed behavior reliability, norm, dominant value, philosophy rule, organization climate. The results of the study indicate that these five dimensions show that Bina Sarana Informatika University has a more dominant culture. Employee performance at Bina Sarana Informatika University is also in a very high category as measured by three dimensions according to the quality of work, quantity, timeliness, effectiveness and independence. In addition, the results also show that there is a positive and significant influence between the organizational culture variable and the employee performance variable. The magnitude of the contribution of organizational culture to the employee performance of Bina Sarana Informatika University employees is 67.3%, while the remaining 37.8% contributes to other factors.