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All Journal Jurnal Pengendalian Hayati Agrovigor: Jurnal Agroekoteknologi Plumula : Berkala Ilmiah Agroteknologi Agrifor : Jurnal Ilmu Pertanian dan Kehutanan Jurnal Ilmu-Ilmu Pertanian Indonesia Jurnal Agroekoteknologi Jurnal Perlindungan Tanaman Indonesia JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA BIOEDUSCIENCE Jurnal Teknik Pertanian Lampung (Journal of Agricultural Engineering) JPT : JURNAL PROTEKSI TANAMAN (JOURNAL OF PLANT PROTECTION) JURNAL AGROHITA Jurnal Pembelajaran dan Biologi Nukleus Jurnal Agrotropika PLAKAT : Jurnal Pelayanan Kepada Masyarakat Nusantara Science and Technology Proceedings Berkala Penelitian Hayati Jurnal Pengabdian Kepada Masyarakat JPMP Agrocentrum Jurnal Bioteknologi & Biosains Indonesia (JBBI)
Yenny WURYANDARI
Fakultas Pertanian Universitas Pembangunan Nasional “Veteran” Jawa Timur
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Civil Engineering, Building, Construction & Architecture Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Education Electrical & Electronics Engineering Energy Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Mechanical Engineering Medicine & Pharmacology Social Sciences Other

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Carrier Materials Differences on Bioencapsulation Formulation Affect Bacillus sp. Isolates Wuryandari, Yenny; Suryaminarsih, Penta; Lestari, Safira Rizka
Nusantara Science and Technology Proceedings 8th International Seminar of Research Month 2023
Publisher : Future Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/nstp.2024.4162

Abstract

Biological agent Bacillus sp. Based on the results of previous research, direct use of isolated microorganisms BCZ14, BCZ16, and BCZ20 was able to suppress Fusarium wilt disease. However, Bacillus sp. formulations in suspension form are less effective when applied in the field. This research focuses on analyzing the viability of three isolates of Bacillus sp. against several combinations of bioencapsulation coating materials. The experimental design used was a completely randomized design with a combination of treatment of three isolates and three types of coating materials, namely sodium alginate (B1), carrageenan (B2), and chitosan (B3) each 2%. Each treatment combination was repeated five times. Based on the experiments that have been carried out, the bioencapsulation formulation using 2% chitosan coating material was able to maintain the viability of the three isolates of Bacillus sp. BCZ14, BCZ16, and BCZ20. The population density of the three isolates of Bacillus sp. persisted consistently from the first week to the last week of observation. Therefore, the 2% chitosan coating material can be said to be able to maintain the viability of Bacillus sp. within six weeks.
Potential of The Biological Agent Bacillus spp in Inhibiting Fusarium Wild Disease and its Effects on The Growth and Production of Cayenne Papper (Capsicum frutescens L.) Zakqy, Nauval; Wuryandari, Yenny; Purnawati, Arika
JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS Vol 10, No 1: Jurnal Pembelajaran Dan Biologi Nukleus March 2024
Publisher : Fakultas Keguruan dan Ilmu Pendidikan Universitas Labuhanbatu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/jpbn.v10i1.5366

Abstract

Cayenne pepper (Capsicum frutescens L.) is a horticultural crop in Indonesia that often has many obstacles. The main obstacle that is often faced is wilt disease caused by the fungus Fusarium oxysporum. Bacillus spp. bacteria have the potential to control the fungus F. oxysporum. The purpose of this study was to determine the ability of Bacillus spp. bacteria in controlling fusarium wilt disease. This research was conducted from August to November 2023 at the Plant Health Laboratory and screen house of the Faculty of Agriculture, National Development University "Veteran" East Java. The study used a Factorial Complete Randomized Block Design with three replications and nine treatments so that there were 27 experimental units. The observation variables included incubation period, disease intensity, plant height, number of leaves, first flower appearance and fruit wet weight. The research data were analyzed using ANOVA analysis of variance procedure to determine whether there is an effect of each treatment. If it is known that there is a significantly different effect from the treatment, it will be continued with the DMRT test at the 5% level. The results showed that the combination of Bacillus spp bacteria and dosage had an effect on the incubation period and disease intensity. The results showed that the combination of Bacillus spp bacteria and doses had an effect on vegetative phase parameters, namely plant height and number of leaves. However, the combination of Bacillus spp and dosage showed that the generative phase parameters did not significantly affect the first flower appearance and fruit wet weight
EVALUATING THE LONG-TERM STORAGE TIME VIABILITY AND SIZE DYNAMICS OF BACILLUS SP. BIOENCAPSULATION IN SODIUM ALGINATE MATRIX Mochammad Mirza Saputra; Yenny Wuryandari; Noni Rahmadhini; Safira Rizka Lestari
Jurnal Bioteknologi dan Biosains Indonesia Vol. 10 No. 2 (2023)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

The use of biological agents such as Bacillus sp. bacteria has begun to be widely used by farmers as a new form of control. Bacillus sp. needs to require special methods in its application because it has certain living conditions, and Bacillus sp. is vulnerable to environmental pressures. Bioencapsulated formulation in the form of granules is considered effective as a form of bacterial application in soil because it is able to protect Bacillus sp. and maintain survival. This study aims to determine the best concentration of sodium alginate in maintaining the viability of Bacillus sp. at the in vitro stage and to see changes in the size of the beads. The beads were made using extrusion method by combining Bacillus sp. suspension and sodium alginate suspension at 1%, 1.5%, and 2% concentration. The concentration of sodium alginate was not a major factor in changes in bead size and viability test results experienced significant changes in each observation. 1.5% and 2% concentrations can reach the highest viability value of 3x106 CFU/mL and decreased during 1 month of observation. Changes in bead size and viability were caused by the alginase enzyme produced by Bacillus sp.
Carrier Materials Differences on Bioencapsulation Formulation Affect Bacillus sp. Isolates Yenny Wuryandari; Penta Suryaminarsih; Safira Rizka Lestari
Nusantara Science and Technology Proceedings 8th International Seminar of Research Month 2023
Publisher : Future Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/nstp.2024.4162

Abstract

Biological agent Bacillus sp. Based on the results of previous research, direct use of isolated microorganisms BCZ14, BCZ16, and BCZ20 was able to suppress Fusarium wilt disease. However, Bacillus sp. formulations in suspension form are less effective when applied in the field. This research focuses on analyzing the viability of three isolates of Bacillus sp. against several combinations of bioencapsulation coating materials. The experimental design used was a completely randomized design with a combination of treatment of three isolates and three types of coating materials, namely sodium alginate (B1), carrageenan (B2), and chitosan (B3) each 2%. Each treatment combination was repeated five times. Based on the experiments that have been carried out, the bioencapsulation formulation using 2% chitosan coating material was able to maintain the viability of the three isolates of Bacillus sp. BCZ14, BCZ16, and BCZ20. The population density of the three isolates of Bacillus sp. persisted consistently from the first week to the last week of observation. Therefore, the 2% chitosan coating material can be said to be able to maintain the viability of Bacillus sp. within six weeks.
Potensi Konsorsium Bakteri Pseudomonad fluorescent dan Bacillus mycoides Terhadap Penyakit Layu Bakteri Secara In-vitro Sagala, Yosua Nathanael Itona; Prasetyawati, Endang Triwahyu; Wuryandari, Yenny
Jurnal Proteksi Tanaman Vol 8 No 2 (2024): December 2024
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jpt.8.2.78-87.2024

Abstract

Bacterial wilt disease caused by Ralstonia solanacearum is a disease in chili pepper plants (Capsicum annuum) that is difficult to control. One technique to prevent bacterial wilt disease is consortium of antagonistic bacteria such as Pseudomonad fluorescent and Bacillus mycoides. This study aimed to determine whether the consortium of Pseudomonad fluorescent pf-142 and Bacillus mycoides can inhibit R. solanacearum better than a single application in-vitro. This study was conducted in a Completely Randomized Design (CRD) with four treatments (Pseudomonad fluorescent pf-142, B. mycoides, Pseudomonad fluorescent pf-142 + B. mycoides, and control) in six replications, resulting in 24 experimental units. The variables observed were the symptoms of attack and pathogenicity of R. solanacearum, compatibility and inhibitory rate of the consortium of Pseudomonad fluorescens pf-142 and B. mycoides against R. solanacearum. Based on the study, it was known that R. solanacearum caused wilting in chili pepper plants with high virulence. Pseudomonad fluorescent pf-142 and B. mycoides did not produce an inhibition zone, indicating that both were compatible. The consortium of Pseudomonad fluorescent pf-142 and B. mycoides provided the widest inhibition zone, indicating strong antagonistic ability against R. solanacearum.
Potential of The Biological Agent Bacillus sp. in Inhibiting Moler Disease and its Effects on The Growth and Production of Shallot (Allium ascalonicum L.) Yuwono, F. Tsara Nafisah; Wuryandari, Yenny; Mujoko, Tri
JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS Vol 11, No 1: Jurnal Pembelajaran Dan Biologi Nukleus March 2025
Publisher : Universitas Labuhanbatu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/jpbn.v11i1.6428

Abstract

Shallot (Allium ascalonicum L.) is one of the important commodities in Indonesia. The main obstacle that is often faced is moler disease caused by Fusarium sp. Bacillus sp. bacteria have the potential to control Fusarium sp. The purpose of study was to determine the ability of Bacillus sp. in controlling moler disease and its effect on the growth and production of onion plants. The study used a factorial completely randomized design with three replications and nine treatments, so there were 27 experimental units. The observation variables included incubation period, disease intensity, plant height, number of leaves, and tuber wet weight. The research data were analyzed using the ANOVA analysis of variance procedure to determine whether there was an effect of each treatment. If it is known that there is a significantly different effect from the treatment, it will be continued with the DMRT test at the 5 % level. The results showed that the combination of Bacillus sp. isolate bcz 16 and dosage 35 ml/polybag was the longest delaying the incubation period of 15 day and could suppress moler disease by 50 %. The treatment of Bacillus sp. isolate bcz 16 and dosage 35 ml also gave the highest results in plant length, number of leaves, and wet weight of shallot bulbs
KEMAMPUAN ANTAGONISTIK BEBERAPA ISOLAT Bacillus spp. TERHADAP PATOGEN Pyricularia oryzae PENYEBAB PENYAKIT BLAS TANAMAN PADI (Oryza sativa L.) PADA BEBERAPA KONSENTRASI Setiawan Setiawan; Yenny Wuryandari; Tr Mujoko
Jurnal Ilmu-Ilmu Pertanian Indonesia Vol 27 No 1 (2025)
Publisher : BPFP Universitas Bengkulu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31186/jipi.27.1.67-76

Abstract

Rice (Oryza sativa L.) is a staple food crop that plays a vital role in supporting national food security. One of the major constraints in rice cultivation is blast disease, caused by the fungal pathogen Pyricularia oryzae, which can significantly reduce crop productivity and lead to substantial economic losses. The conventional management of this disease relies heavily on chemical pesticides, which pose long-term risks to environmental and human health. Therefore, eco-friendly alternatives such as biological control agents are necessary, with Bacillus spp. being one promising candidate. This study aimed to evaluate the antagonistic activity of three Bacillus spp. isolates (Bcz-14, Bcz-20, and Bcz-30) against P. oryzae at two bacterial population densities (10⁶ CFU/mL and 10⁹ CFU/mL) under in vitro conditions. The experiment was conducted at the Plant Health Laboratory, Faculty of Agriculture, Universitas Pembangunan Nasional “Veteran” East Java, from January to March 2025. Results of the antagonism assay demonstrated that all Bacillus spp. isolates inhibited the growth of P. oryzae, with the highest inhibition observed in treatments Bcz-14 and Bcz-30 at 10⁶ CFU/mL, with inhibition rates of 24.29% and 23.35%, respectively. The observed inhibition mechanisms included competition and antibiosis. Microscopic observations revealed abnormal hyphal structures in P. oryzae, including lysis, swelling, and chlamydospore formation, in response to Bacillus spp. treatment.  
EFFECT OF GROWING MEDIA ON THE GROWTH OF Streptomyces sp. AND Trichoderma harzianum AS ANTHRACNOSE DISEASE BIOCONTROL Rulinggar, Nia; Mujoko, Tri; Wuryandari, Yenny
Jurnal Bioteknologi dan Biosains Indonesia Vol. 12 No. 1 (2025)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

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Abstract

Streptomyces sp. and Trichoderma harzianum is a biological agent that is effective in controlling Colletotrichum capsici, the cause of disease in chili plants. Different growing media can affect the growth and activity of these two microorganisms. This study aims to evaluate the growth of Streptomyces sp. and Trichoderma harzianum on various media, namely rice washing water, coconut water, and sugar potato extract. The research phase includes growing media test, effectiveness test of Streptomyces sp. and Trichoderma harzianum in chili peppers, and effectiveness test of Streptomyces sp. and Trichoderma harzianum on chili seeds. The results showed that the medium of rice washing water is more suitable for Streptomyces sp., coconut water media is more suitable for Trichoderma harzianum, and rice washing water media is more suitable for the combination of the two biological agents. Optimal growth in these media affect the effectiveness of biocontrol in suppressing the severity of anthracnose disease in chili.
Exploration Of Rice Plant Endophyte Bacteria To Suppress Xanthomonas oryzae Bacterial Leaf Blight Disease Putri, Ratih Nadhila; Wuryandari, Yenny; Wahyu, Endang Tri
JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS Vol 11, No 2: Jurnal Pembelajaran Dan Biologi Nukleus June 2025
Publisher : Universitas Labuhanbatu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/jpbn.v11i2.7338

Abstract

Background: Rice is a major food commodity for Indonesian. According to BPS (2022) rice production in 2021 decreased 0.43%. The decline caused by various factors, one of which is bacterial leaf blight disease by the pathogenic bacteria Xanthomonas oryzae. Control of X. oryzae mostly uses pesticides, but this control has a negative impact on the environment, therefore it is necessary to develop endophytic bacteria (EB) that can inhibit the growth of X. oryzae and can act as PGPR. This study was to determine the EB ability in healthy rice stem tissue to suppress bacterial leaf blight disease caused by the bacteria X. oryzae. Methodology: This research was were used isolation from infected plant for X. oryzae and from healthy plant for EB, data was analyzed using the corresponding formula. Findings: found 10 isolates of EB that could inhibit the X. oryzae growth as seen from the inhibition zone produced in the antagonist test. The EB isolates produced the largest inhibition zones such as BE18 (23.3 mm, very strong category), BE16 (13.3 mm, strong category), and BE3 (11.6 mm, strong category). The 4 isolates with bactericidal inhibition mechanisms, namely isolates (BE11, BE15, BE16, and BE18), 6 isolates with bacteriostatic inhibition mechanisms, namely isolates (BE3, BE5, BE6, BE7, BE12, and BE13). Contribution: The availability of EB present in the tissues of healthy rice stems is still relatively high and can be used to suppress the growth of pathogenic bacterium X. oryzae. This rseult also supported by the beneficial properties of EB, which can enhance plant resistance
Rebranding Produk Kopi Excelsa Oleh Kelompok Masyarakat KOPI GUNUNG Dengan Packaging Baru dan Digital Marketing Achmad, Zainal Abidin; Wuryandari, Yenny; Mas’udah, Kusuma Wardhani; Tamrin, M. Husni
Plakat : Jurnal Pelayanan Kepada Masyarakat Vol 4, No 2 (2022): Volume 4, Nomor 2 Desember Tahun 2022
Publisher : Fakultas Ilmu Sosial dan Ilmu Politik, Universitas Mulawarman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30872/plakat.v4i2.8899

Abstract

Coffee is a leading commodity in Jombang Regency. One of these coffee varieties is Excelsa Coffee. The main obstacle for coffee farmers and traders in Sumberjo Village is the processing of excelsa coffee which is quite complicated. The Grant Program for Application of Research Results for the Community (PIHAT) aims to foster community groups KOPI GUNUNG in Sumberjo Village, Wonosalam District, Jombang Regency to have knowledge and skills in coffee plant care and digital marketing. The results of the identification of the problem of Pokmas KOPI GUNUNG as a partner, the PIHAT team provides solutions in the form of a) Increasing knowledge and skills in caring for coffee plants, b) Improving the quality of new labels and packaging, c) Creating Instagram accounts and making marketing support profile videos, and d) Digital marketing training. After partners gain knowledge and skills in caring for coffee plants, knowledge of digital marketing skills, and producing coffee product packaging, the goal of implementing this PIHAT program is to increase sales of coffee products from the KOPI GUNUNG community group with the brand and packaging "Cakra Dewa." In addition to articles in community service journals, PIHAT's outputs are the Profile Video, news, and Instagram accounts. Kopi merupakan komoditas unggulan Kabupaten Jombang. Salah satu varietas kopi tersebut adalah Kopi Excelsa. Kendala utama bagi petani dan pedagang kopi di Desa Sumberjo adalah perawatan kopi excelsa yang cukup rumit. Program Hibah Penerapan Hasil Penelitian Bagi Masyarakat (PIHAT) bertujuan untuk membina kelompok masyarakat KOPI GUNUNG di Desa Sumberjo, Kecamatan Wonosalam, Kabupaten Jombang agar memiliki pengetahuan dan keterampilan dalam perawatan tanaman kopi dan pemasaran digital. Hasil identifikasi masalah Pokmas KOPI GUNUNG sebagai mitra, maka tim PIHAT memberikan solusi berupa: a) Meningkatkan pengetahuan dan keterampilan dalam merawat tanaman kopi, b) Meningkatkan kualitas label dan kemasan baru, c) Membuat akun Instagram dan membuat video profil penunjang pemasaran, dan d) Pelatihan pemasaran digital. Setelah mitra mendapatkan pengetahuan dan keterampilan dalam merawat tanaman kopi, pengetahuan keterampilan digital marketing, dan memproduksi kemasan produk kopi, maka sasaran akhir dari pelaksanaan program PIHAT ini adalah meningkatkan penjualan produk kopi dari kelompok masyarakat KOPI GUNUNG dengan brand dan kemasan “Cakra Dewa.” Selain artikel pada jurnal pengabdian kepada masyarakat, luaran PIHAT ini adalah Video Profil, Berita, dan akun Instagram.
Co-Authors Achmad Fiqri Agus Wahyudi Aisyah Lulu Hariyanto Anjani, Ajeng Distya arfah Arika Purnawati Bambang HADISUTRISNO Bambang Hadisutrisno Budi Rianto Devi Tria Anjarsari Devi Tria Anjarsari Didiek Tranggono Edo Pandu Pratama Fadia Nur Rahma Budiarto Frida Nur Aisah Gita Pawana Hera Amalia Putri Belangi Herry Nirwanto HIDAYATI, KIKI I. Hartana Indah Sari Dwi Agustin Indarwati Indarwati Kaswan Badami Kiki Sandra Amelia Kusuma Wardhani Mas'udah Lestari, Safira Rizka M. Husni Tamrin, M. Husni Maroeto ., Maroeto Maroeto Maroeto Mas’udah, Kusuma Wardhani Mochammad Mirza Saputra Munir, Ahmad Arsyad Nauval Zakqy Noni Rahmadhini Noni Rahmadhini Nova Andriani Novita Endah Ariani Nuriatus Sholikhah Penta SURYAMINARSIH Perwira Moekti, Nirwana Septania Galih Prasetyawati, Endang Triwahyu Putranto Sasikirono Putri, Ratih Nadhila Raharja, Wildan Taufik Rahmadhini, Noni Ratu Yusnita Zhalsabilla Rulinggar, Nia Safira Rizka Lestari Safira Rizka Lestari Sagala, Yosua Nathanael Itona Saputra, Mochammad Mirza Setiawan Setiawan Shelomitha Kumala Mawardhany Sherly Arindawati Sri Wiyatiningsih Sulistyanto Sulistyawati, Hanik Suwandi Syamsul Arifin Taufik Hidayat Tr Mujoko Tri MUJOKO Triwidodo ARWIYANTO Triwidodo Arwiyanto Wahyu, Endang Tri Windriyanti, Wiwin Yovita Nathania Yuwono, F. Tsara Nafisah Zainal Abidin Achmad Zakqy, Nauval