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All Journal Jurnal Pengendalian Hayati Agrovigor: Jurnal Agroekoteknologi Plumula : Berkala Ilmiah Agroteknologi Agrifor : Jurnal Ilmu Pertanian dan Kehutanan Jurnal Ilmu-Ilmu Pertanian Indonesia Jurnal Agroekoteknologi Jurnal Perlindungan Tanaman Indonesia JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Jurnal Ilmiah Universitas Batanghari Jambi BIOEDUSCIENCE Jurnal Teknik Pertanian Lampung (Journal of Agricultural Engineering) JPT : JURNAL PROTEKSI TANAMAN (JOURNAL OF PLANT PROTECTION) JURNAL AGROHITA Jurnal Pembelajaran dan Biologi Nukleus Jurnal Agrotropika PLAKAT : Jurnal Pelayanan Kepada Masyarakat Nusantara Science and Technology Proceedings Bioscientist : Jurnal Ilmiah Biologi Berkala Penelitian Hayati JPMP Agrocentrum Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Bioteknologi & Biosains Indonesia
Yenny WURYANDARI
Program Studi Agroteknologi, Fakultas Pertanian, UPN “Veteran” Jawa Timur
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Civil Engineering, Building, Construction & Architecture Control & Systems Engineering Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Education Energy Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Medicine & Pharmacology Social Sciences Other

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Promotion of Beauveria bassiana Mushroom on Different Growing Media and Its Pathogenicity on Insects Lukmanul Hakim; Yenny Wuryandari
Nusantara Science and Technology Proceedings Seminar Nasional Agroteknologi 2022
Publisher : Future Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/nstp.2023.3116

Abstract

One type of entomopathogenic fungus that is known to be effective in controlling important plant pests is the Beauveria bassiana fungus. The fungus B. bassiana, has a high reproductive capacity, is easy to produce and under unfavorable conditions can form spores that can last a long time in nature. The purpose of this study was to determine the density and viability of B. bassiana spores on corn and rice media and to investigate the process of B. bassiana fungal infection in the test insects. The method used is; Fungal propagation test on Rice and Corn Media, Microscopic Observation of Fungus from Corn and Rice Media, Calculation of Fungus Spore Density, Calculation of Fungus Viability and Pathogenicity Test. The research results obtained are as follows; Results The propagation of B. bassiana mushroom grew on the seventh day, the fungus growth was faster in rice media than in corn media. The spore density of the fungus B. bassiana clove bondowoso in corn media was 3.75x108 spores/ml, rice media was 4.58x108 spores/ml, B. bassiana banyuwangi coffee medium was 3.5x108 spores/ml, rice media was 3.75x108 spores/ml. The viability of the fungus B. bassiana, clove bondowoso and banyuwangi coffee on rice and corn media was obtained on average above 60%. The results of the pathogenicity test of B. bassiana on insects began to appear on the tenth day after the baiting process.
Potensi Konsorsium Bakteri Pseudomonad fluorescent dan Bacillus mycoides Terhadap Penyakit Layu Bakteri Secara In-vitro Sagala, Yosua Nathanael Itona; Prasetyawati, Endang Triwahyu; Wuryandari, Yenny
Jurnal Proteksi Tanaman Vol 8 No 2 (2024): December 2024
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jpt.8.2.78-87.2024

Abstract

Bacterial wilt disease caused by Ralstonia solanacearum is a disease in chili pepper plants (Capsicum annuum) that is difficult to control. One technique to prevent bacterial wilt disease is consortium of antagonistic bacteria such as Pseudomonad fluorescent and Bacillus mycoides. This study aimed to determine whether the consortium of Pseudomonad fluorescent pf-142 and Bacillus mycoides can inhibit R. solanacearum better than a single application in-vitro. This study was conducted in a Completely Randomized Design (CRD) with four treatments (Pseudomonad fluorescent pf-142, B. mycoides, Pseudomonad fluorescent pf-142 + B. mycoides, and control) in six replications, resulting in 24 experimental units. The variables observed were the symptoms of attack and pathogenicity of R. solanacearum, compatibility and inhibitory rate of the consortium of Pseudomonad fluorescens pf-142 and B. mycoides against R. solanacearum. Based on the study, it was known that R. solanacearum caused wilting in chili pepper plants with high virulence. Pseudomonad fluorescent pf-142 and B. mycoides did not produce an inhibition zone, indicating that both were compatible. The consortium of Pseudomonad fluorescent pf-142 and B. mycoides provided the widest inhibition zone, indicating strong antagonistic ability against R. solanacearum.
KEMAMPUAN ANTAGONISTIK BEBERAPA ISOLAT Bacillus spp. TERHADAP PATOGEN Pyricularia oryzae PENYEBAB PENYAKIT BLAS TANAMAN PADI (Oryza sativa L.) PADA BEBERAPA KONSENTRASI Setiawan Setiawan; Yenny Wuryandari; Tr Mujoko
Jurnal Ilmu-Ilmu Pertanian Indonesia Vol 27 No 1 (2025)
Publisher : BPFP Universitas Bengkulu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31186/jipi.27.1.67-76

Abstract

Rice (Oryza sativa L.) is a staple food crop that plays a vital role in supporting national food security. One of the major constraints in rice cultivation is blast disease, caused by the fungal pathogen Pyricularia oryzae, which can significantly reduce crop productivity and lead to substantial economic losses. The conventional management of this disease relies heavily on chemical pesticides, which pose long-term risks to environmental and human health. Therefore, eco-friendly alternatives such as biological control agents are necessary, with Bacillus spp. being one promising candidate. This study aimed to evaluate the antagonistic activity of three Bacillus spp. isolates (Bcz-14, Bcz-20, and Bcz-30) against P. oryzae at two bacterial population densities (10⁶ CFU/mL and 10⁹ CFU/mL) under in vitro conditions. The experiment was conducted at the Plant Health Laboratory, Faculty of Agriculture, Universitas Pembangunan Nasional “Veteran” East Java, from January to March 2025. Results of the antagonism assay demonstrated that all Bacillus spp. isolates inhibited the growth of P. oryzae, with the highest inhibition observed in treatments Bcz-14 and Bcz-30 at 10⁶ CFU/mL, with inhibition rates of 24.29% and 23.35%, respectively. The observed inhibition mechanisms included competition and antibiosis. Microscopic observations revealed abnormal hyphal structures in P. oryzae, including lysis, swelling, and chlamydospore formation, in response to Bacillus spp. treatment.  
EFFECT OF GROWING MEDIA ON THE GROWTH OF Streptomyces sp. AND Trichoderma harzianum AS ANTHRACNOSE DISEASE BIOCONTROL Rulinggar, Nia; Mujoko, Tri; Wuryandari, Yenny
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 12 No. 1 (2025)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2025.9257

Abstract

Streptomyces sp. and Trichoderma harzianum is a biological agent that is effective in controlling Colletotrichum capsici, the cause of disease in chili plants. Different growing media can affect the growth and activity of these two microorganisms. This study aims to evaluate the growth of Streptomyces sp. and Trichoderma harzianum on various media, namely rice washing water, coconut water, and sugar potato extract. The research phase includes growing media test, effectiveness test of Streptomyces sp. and Trichoderma harzianum in chili peppers, and effectiveness test of Streptomyces sp. and Trichoderma harzianum on chili seeds. The results showed that the medium of rice washing water is more suitable for Streptomyces sp., coconut water media is more suitable for Trichoderma harzianum, and rice washing water media is more suitable for the combination of the two biological agents. Optimal growth in these media affect the effectiveness of biocontrol in suppressing the severity of anthracnose disease in chili.
Antagonist Test of Streptomyces spp. from Shallot Fields in Bojonegoro Against Fusarium sp. Which Causes Moler Disease In vitro Hidayati, Kiki; Mujoko, Tri; Wuryandari, Yenny
BIOEDUSCIENCE Vol 9 No 1 (2025): BIOEDUSCIENCE
Publisher : Universitas Muhammadiyah Prof. Dr. Hamka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22236/jbes/15473

Abstract

Background: Background: Shallots are a horticultural product widely consumed in Indonesia. In 2021, shallot production will decline. This is due to pest and disease attacks on plants. Fusarium sp. is a pathogen that causes the primary disease in shallots, namely moler disease, which can cause losses of up to 50%, so control activities must be carried out. This research aims to determine the potential of Streptomyces sp. bacteria from exploring shallot planting areas in Bojonegoro against Fusarium sp. fungi in vitro. Methods: This study used 15 isolates of Streptomyces sp. from exploration results tested with Fusarium sp. using the dual culture method, and negative control (only Fusarium sp.) repeated 3 times for each treatment. Observations were carried out every day for 7 days by observing the growth of Fusarium sp. hyphae and their inhibitory power. Result: The research showed that isolate S8 had the highest inhibitory power, 49.5%. Meanwhile, the one with the lowest results was isolated S1, 34.5%. From the chitinase test results, the chitinocytic index of isolate S8 was 5.2, which is in the high category. Meanwhile, the isolate S8 cellulolytic index was 4.8 and was classified as high. Conclusions: All Streptomyces sp.p isolates inhibited the growth of Fusarium sp., which was characterized by an inhibition zone compared to the control treatment.
PENGARUH ENKAPSULASI BENIH CABAI RAWIT (Capsicum frutescens L.) DENGAN BERBAGAI BAHAN PEMBAWA PADA BAHAN AKTIF Trichoderma spp. UNTUK PENGENDALIAN PENYAKIT LAYU FUSARIUM Hidayat, Taufik; Nirwanto, Herry; Wuryandari, Yenny
JURNAL AGROTROPIKA Vol. 24 No. 1 (2025): Jurnal Agrotropika Vol 24 No 1, Mei 2025
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/ja.v24i1.8810

Abstract

Fusarium wilt is an important disease of cayenne pepper plants caused by the fungus Fusarium. Trichoderma is a type of biological agent that can be used to control plant diseases, one of which is fusarium wilt. This research was conducted to determine the effect of seed encapsulation with different carrier materials and the active ingredient Trichoderma in controlling fusarium wilt disease. This research was carried out in September 2023-January 2024, at the Plant Health Laboratory and Green House of UPN "Veteran" East Java. This research was prepared using a Completely Randomized Design (CRD) using 7 treatments and 3 replications. The data obtained were analyzed using analysis of variance with a further 5% DMRT test. The research results showed that the use of carrier materials and active ingredients of Trichoderma  influence on ability to control the fusarium wilt of cayenne pepper plants. The resulting inhibitory ability is categorized as capable of controlling fusarium wilt disease with an inhibitory ability of >50% (In vitro) and in vivo can reduce the intensity of the attack to <40%. Carrier materials glutinous flour (in vitro) and compost (in vivo) with the active ingredient Trichoderma harzianum are the best carrier materials in controlling fusarium wilt disease in cayenne pepper plants.
Eksplorasi dan Uji Antagonis Bakteri Endofit Dari Daun Tanaman Mangrove Avicennia sp. Terhadap Jamur Patogen Alteria sp. Secara In Vitro Perwira Moekti, Nirwana Septania Galih; Prasetyawati, Endang Triwahyu; Wuryandari, Yenny
Jurnal Agroekoteknologi Vol 17, No 1 (2025)
Publisher : Jurusan Agroekoteknologi Fakultas Pertanian Untirta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33512/jur.agroekotetek.v17i1.30972

Abstract

Mangrove (Avicennia sp.) merupakan salah satu ekosistem penting yang sering ditemukan di muara sungai yang memiliki kemampuan untuk tumbuh dan berkembang di zona salinitas tinggi. Salah satu jenis tanaman mangrove yang bermanfaat dan terdapat berbagai jenis bakteri endofit adalah Avicennia marina. Oleh karena itu, penelitian ini bertujuan untuk mendapatkan isolat bakteri endofit yang berpotensi sebagai agensia hayati pada daun tanaman bakau Avicennia sp. terhadap cendawan patogen Alternaria sp. Metode isolasi yang digunakan adalah penanaman langsung. Pengujian antagonis dilakukan dengan metode dual culture melalui pengukuran diameter zona hambat. Sampel yang diambil adalah daun yang sehat dan segar. Bakteri endofit yang diperoleh sebanyak 24 isolat bakteri yang memiliki morfologi yang berbeda. Diameter zona hambat dengan kategori kuat adalah isolat D28 sebesar 12,5 mm dengan kategori kuat dan kategori sedang adalah isolat D13, D19 sebesar 7,5 mm; 8,5 mm.  Mekanisme kerja bakteri endofit adalah antibiosis dan kompetisi ruang. Hasil penelitian ini dapat disimpulkan bahwa terdapat bakteri endofit dari daun mangrove Avicennia sp. yang berpotensi sebagai antijamur terhadap jamur patogen Alternaria sp.
EVALUATING THE LONG-TERM STORAGE TIME VIABILITY AND SIZE DYNAMICS OF BACILLUS SP. BIOENCAPSULATION IN SODIUM ALGINATE MATRIX Saputra, Mochammad Mirza; Wuryandari, Yenny; Rahmadhini, Noni; Lestari, Safira Rizka
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 10 No. 2 (2023)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2023.2549

Abstract

The use of biological agents such as Bacillus sp. bacteria has begun to be widely used by farmers as a new form of control. Bacillus sp. needs to require special methods in its application because it has certain living conditions, and Bacillus sp. is vulnerable to environmental pressures. Bioencapsulated formulation in the form of granules is considered effective as a form of bacterial application in soil because it is able to protect Bacillus sp. and maintain survival. This study aims to determine the best concentration of sodium alginate in maintaining the viability of Bacillus sp. at the in vitro stage and to see changes in the size of the beads. The beads were made using extrusion method by combining Bacillus sp. suspension and sodium alginate suspension at 1%, 1.5%, and 2% concentration. The concentration of sodium alginate was not a major factor in changes in bead size and viability test results experienced significant changes in each observation. 1.5% and 2% concentrations can reach the highest viability value of 3x106 CFU/mL and decreased during 1 month of observation. Changes in bead size and viability were caused by the alginase enzyme produced by Bacillus sp.
EFFECT OF GROWING MEDIA ON THE GROWTH OF Streptomyces sp. AND Trichoderma harzianum AS ANTHRACNOSE DISEASE BIOCONTROL Rulinggar, Nia; Mujoko, Tri; Wuryandari, Yenny
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 12 No. 1 (2025)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2025.9257

Abstract

Streptomyces sp. and Trichoderma harzianum is a biological agent that is effective in controlling Colletotrichum capsici, the cause of disease in chili plants. Different growing media can affect the growth and activity of these two microorganisms. This study aims to evaluate the growth of Streptomyces sp. and Trichoderma harzianum on various media, namely rice washing water, coconut water, and sugar potato extract. The research phase includes growing media test, effectiveness test of Streptomyces sp. and Trichoderma harzianum in chili peppers, and effectiveness test of Streptomyces sp. and Trichoderma harzianum on chili seeds. The results showed that the medium of rice washing water is more suitable for Streptomyces sp., coconut water media is more suitable for Trichoderma harzianum, and rice washing water media is more suitable for the combination of the two biological agents. Optimal growth in these media affect the effectiveness of biocontrol in suppressing the severity of anthracnose disease in chili.
Rebranding Produk Kopi Excelsa Oleh Kelompok Masyarakat KOPI GUNUNG Dengan Packaging Baru dan Digital Marketing Zainal Abidin Achmad; Yenny Wuryandari; Kusuma Wardhani Mas’udah; M. Husni Tamrin
Plakat : Jurnal Pelayanan Kepada Masyarakat Vol 4, No 2 (2022): Plakat: Jurnal Pelayanan Kepada Masyarakat
Publisher : Fakultas Ilmu Sosial dan Ilmu Politik, Universitas Mulawarman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30872/plakat.v4i2.8899

Abstract

Coffee is a leading commodity in Jombang Regency. One of these coffee varieties is Excelsa Coffee. The main obstacle for coffee farmers and traders in Sumberjo Village is the processing of excelsa coffee which is quite complicated. The Grant Program for Application of Research Results for the Community (PIHAT) aims to foster community groups KOPI GUNUNG in Sumberjo Village, Wonosalam District, Jombang Regency to have knowledge and skills in coffee plant care and digital marketing. The results of the identification of the problem of Pokmas KOPI GUNUNG as a partner, the PIHAT team provides solutions in the form of a) Increasing knowledge and skills in caring for coffee plants, b) Improving the quality of new labels and packaging, c) Creating Instagram accounts and making marketing support profile videos, and d) Digital marketing training. After partners gain knowledge and skills in caring for coffee plants, knowledge of digital marketing skills, and producing coffee product packaging, the goal of implementing this PIHAT program is to increase sales of coffee products from the KOPI GUNUNG community group with the brand and packaging "Cakra Dewa." In addition to articles in community service journals, PIHAT's outputs are the Profile Video, news, and Instagram accounts. Kopi merupakan komoditas unggulan Kabupaten Jombang. Salah satu varietas kopi tersebut adalah Kopi Excelsa. Kendala utama bagi petani dan pedagang kopi di Desa Sumberjo adalah perawatan kopi excelsa yang cukup rumit. Program Hibah Penerapan Hasil Penelitian Bagi Masyarakat (PIHAT) bertujuan untuk membina kelompok masyarakat KOPI GUNUNG di Desa Sumberjo, Kecamatan Wonosalam, Kabupaten Jombang agar memiliki pengetahuan dan keterampilan dalam perawatan tanaman kopi dan pemasaran digital. Hasil identifikasi masalah Pokmas KOPI GUNUNG sebagai mitra, maka tim PIHAT memberikan solusi berupa: a) Meningkatkan pengetahuan dan keterampilan dalam merawat tanaman kopi, b) Meningkatkan kualitas label dan kemasan baru, c) Membuat akun Instagram dan membuat video profil penunjang pemasaran, dan d) Pelatihan pemasaran digital. Setelah mitra mendapatkan pengetahuan dan keterampilan dalam merawat tanaman kopi, pengetahuan keterampilan digital marketing, dan memproduksi kemasan produk kopi, maka sasaran akhir dari pelaksanaan program PIHAT ini adalah meningkatkan penjualan produk kopi dari kelompok masyarakat KOPI GUNUNG dengan brand dan kemasan “Cakra Dewa.” Selain artikel pada jurnal pengabdian kepada masyarakat, luaran PIHAT ini adalah Video Profil, Berita, dan akun Instagram.
Co-Authors Achmad Fiqri Agus Wahyudi Aisyah Lulu Hariyanto Ajeng Distya Anjani Annisa Zukhruf Oktavia Salamah arfah Arika Purnawati Aullyvia Putri Nabiella Azzura Talitha Maulidya Irawan Bambang HADISUTRISNO Bambang Hadisutrisno Budi Rianto Devi Tria Anjarsari Devi Tria Anjarsari Didiek Tranggono Edo Pandu Pratama Endang Tri Wahyu Erlyana Desy Rahmawati Fadia Nur Rahma Budiarto Frida Nur Aisah Gita Pawana Hera Amalia Putri Belangi Herry Nirwanto Herry Nirwanto HIDAYATI, KIKI I. Hartana Indah Sari Dwi Agustin Indarwati Indarwati Kaswan Badami Kiki Sandra Amelia Kusuma Wardhani Mas’udah Kusumawati, Diah Budi Lukmanul Hakim M. Husni Tamrin Maroeto ., Maroeto Maroeto Maroeto Mochammad Mirza Saputra Mochammad Mirza Saputra Muhammad Qoyyimul Amri Munir, Ahmad Arsyad Nauval Zakqy Nirwana Septania Galih Perwira Moekti Nolla Dwi Elvira Noni Rahmadhini Noni Ramadhini Nova Andriani Novita Endah Ariani Novita Endah Ariani Nuriatus Sholikhah Penta SURYAMINARSIH Perwira Moekti, Nirwana Septania Galih Prasetyawati, Endang Tri Wahyu Prasetyawati, Endang Triwahyu Pratama, Andhika Wahyu Puernawati, Arika Putranto Sasikirono Ratih Nadhila Putri Ratu Yusnita Zhalsabilla Rulinggar, Nia Safira Rizka Lestari Sagala, Yosua Nathanael Itona Salma Alfiani Nadiva Saputra, Mochammad Mirza Saputra, Mochammad Mirza Setiawan Setiawan Shelomitha Kumala Mawardhany Sherly Arindawati Simorangkir, Laura Enjelika Sri Wiyatiningsih Sulistyanto Suwandi Syamsul Arifin Taufik Hidayat Tr Mujoko Tri MUJOKO Triwidodo ARWIYANTO Triwidodo Arwiyanto Wildan Taufik Raharja Windriyanti, Wiwin Yovita Nathania Zainal Abidin Achmad Zakqy, Nauval