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All Journal Jurnal Pengendalian Hayati Agrovigor: Jurnal Agroekoteknologi Plumula : Berkala Ilmiah Agroteknologi Agrifor : Jurnal Ilmu Pertanian dan Kehutanan Jurnal Ilmu-Ilmu Pertanian Indonesia Jurnal Agroekoteknologi Jurnal Perlindungan Tanaman Indonesia JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Jurnal Ilmiah Universitas Batanghari Jambi BIOEDUSCIENCE Jurnal Teknik Pertanian Lampung (Journal of Agricultural Engineering) JPT : JURNAL PROTEKSI TANAMAN (JOURNAL OF PLANT PROTECTION) JURNAL AGROHITA Jurnal Pembelajaran dan Biologi Nukleus Jurnal Agrotropika PLAKAT : Jurnal Pelayanan Kepada Masyarakat Nusantara Science and Technology Proceedings Bioscientist : Jurnal Ilmiah Biologi Berkala Penelitian Hayati Jurnal Pengabdian Kepada Masyarakat JPMP Agrocentrum Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Bioteknologi & Biosains Indonesia
Yenny WURYANDARI
Program Studi Agroteknologi, Fakultas Pertanian, UPN “Veteran” Jawa Timur
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Civil Engineering, Building, Construction & Architecture Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Education Electrical & Electronics Engineering Energy Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Mechanical Engineering Medicine & Pharmacology Social Sciences Other

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Carrier Materials Differences on Bioencapsulation Formulation Affect Bacillus sp. Isolates Yenny Wuryandari; Penta Suryaminarsih; Safira Rizka Lestari
Nusantara Science and Technology Proceedings 8th International Seminar of Research Month 2023
Publisher : Future Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/nstp.2024.4162

Abstract

Biological agent Bacillus sp. Based on the results of previous research, direct use of isolated microorganisms BCZ14, BCZ16, and BCZ20 was able to suppress Fusarium wilt disease. However, Bacillus sp. formulations in suspension form are less effective when applied in the field. This research focuses on analyzing the viability of three isolates of Bacillus sp. against several combinations of bioencapsulation coating materials. The experimental design used was a completely randomized design with a combination of treatment of three isolates and three types of coating materials, namely sodium alginate (B1), carrageenan (B2), and chitosan (B3) each 2%. Each treatment combination was repeated five times. Based on the experiments that have been carried out, the bioencapsulation formulation using 2% chitosan coating material was able to maintain the viability of the three isolates of Bacillus sp. BCZ14, BCZ16, and BCZ20. The population density of the three isolates of Bacillus sp. persisted consistently from the first week to the last week of observation. Therefore, the 2% chitosan coating material can be said to be able to maintain the viability of Bacillus sp. within six weeks.
Utilization of Neem Leaves as a Biological Pesticide for the Control of Anthrachnose Diseases in Dragons (Hylocereus sp.) Nolla Dwi Elvira; Yenny Wuryandari
Nusantara Science and Technology Proceedings Seminar Nasional Agroteknologi 2022
Publisher : Future Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/nstp.2023.3109

Abstract

Dragon fruit (Hylocereus sp.) has problems in its cultivation related to Plant Pest Organisms (OPT). One of the pathogens that attack dragon fruit plants is Colletotrichum sp. which can cause anthracnose. dragon fruit pest control method can be done by using biological pesticides from neem leaf extract. Pesticides from plant extracts are sources of various compounds that are rich in various types of active ingredients such as secondary metabolites that play an important role in the process of interacting or competing and protecting themselves from competitors. The purpose of this study was to determine the effectiveness of neem leaf pesticide for controlling anthracnose on dragon fruit plants. The method used is testing the inhibitory power of neem leaf biological pesticides against anthracnose on dragon fruit plants in vitro. The concentration treatments of biological pesticides included 0%, 20%, 40%, and 60%. The results obtained showed that the biological pesticide neem leaf with a concentration of 20% already had an inhibitory power against the pathogen Colletotrichum sp. which is quite good because at that concentration it already has a fairly high antifungal compound. The magnitude of the inhibitory power of neem leaf biological pesticides at a concentration of 20% was 53.94%.
Promotion of Beauveria bassiana Mushroom on Different Growing Media and Its Pathogenicity on Insects Lukmanul Hakim; Yenny Wuryandari
Nusantara Science and Technology Proceedings Seminar Nasional Agroteknologi 2022
Publisher : Future Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/nstp.2023.3116

Abstract

One type of entomopathogenic fungus that is known to be effective in controlling important plant pests is the Beauveria bassiana fungus. The fungus B. bassiana, has a high reproductive capacity, is easy to produce and under unfavorable conditions can form spores that can last a long time in nature. The purpose of this study was to determine the density and viability of B. bassiana spores on corn and rice media and to investigate the process of B. bassiana fungal infection in the test insects. The method used is; Fungal propagation test on Rice and Corn Media, Microscopic Observation of Fungus from Corn and Rice Media, Calculation of Fungus Spore Density, Calculation of Fungus Viability and Pathogenicity Test. The research results obtained are as follows; Results The propagation of B. bassiana mushroom grew on the seventh day, the fungus growth was faster in rice media than in corn media. The spore density of the fungus B. bassiana clove bondowoso in corn media was 3.75x108 spores/ml, rice media was 4.58x108 spores/ml, B. bassiana banyuwangi coffee medium was 3.5x108 spores/ml, rice media was 3.75x108 spores/ml. The viability of the fungus B. bassiana, clove bondowoso and banyuwangi coffee on rice and corn media was obtained on average above 60%. The results of the pathogenicity test of B. bassiana on insects began to appear on the tenth day after the baiting process.
Potensi Konsorsium Bakteri Pseudomonad fluorescent dan Bacillus mycoides Terhadap Penyakit Layu Bakteri Secara In-vitro Sagala, Yosua Nathanael Itona; Prasetyawati, Endang Triwahyu; Wuryandari, Yenny
Jurnal Proteksi Tanaman Vol 8 No 2 (2024): December 2024
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jpt.8.2.78-87.2024

Abstract

Bacterial wilt disease caused by Ralstonia solanacearum is a disease in chili pepper plants (Capsicum annuum) that is difficult to control. One technique to prevent bacterial wilt disease is consortium of antagonistic bacteria such as Pseudomonad fluorescent and Bacillus mycoides. This study aimed to determine whether the consortium of Pseudomonad fluorescent pf-142 and Bacillus mycoides can inhibit R. solanacearum better than a single application in-vitro. This study was conducted in a Completely Randomized Design (CRD) with four treatments (Pseudomonad fluorescent pf-142, B. mycoides, Pseudomonad fluorescent pf-142 + B. mycoides, and control) in six replications, resulting in 24 experimental units. The variables observed were the symptoms of attack and pathogenicity of R. solanacearum, compatibility and inhibitory rate of the consortium of Pseudomonad fluorescens pf-142 and B. mycoides against R. solanacearum. Based on the study, it was known that R. solanacearum caused wilting in chili pepper plants with high virulence. Pseudomonad fluorescent pf-142 and B. mycoides did not produce an inhibition zone, indicating that both were compatible. The consortium of Pseudomonad fluorescent pf-142 and B. mycoides provided the widest inhibition zone, indicating strong antagonistic ability against R. solanacearum.
Potential of The Biological Agent Bacillus sp. in Inhibiting Moler Disease and its Effects on The Growth and Production of Shallot (Allium ascalonicum L.) Yuwono, F. Tsara Nafisah; Wuryandari, Yenny; Mujoko, Tri
JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS Vol 11, No 1: Jurnal Pembelajaran Dan Biologi Nukleus March 2025
Publisher : Universitas Labuhanbatu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/jpbn.v11i1.6428

Abstract

Shallot (Allium ascalonicum L.) is one of the important commodities in Indonesia. The main obstacle that is often faced is moler disease caused by Fusarium sp. Bacillus sp. bacteria have the potential to control Fusarium sp. The purpose of study was to determine the ability of Bacillus sp. in controlling moler disease and its effect on the growth and production of onion plants. The study used a factorial completely randomized design with three replications and nine treatments, so there were 27 experimental units. The observation variables included incubation period, disease intensity, plant height, number of leaves, and tuber wet weight. The research data were analyzed using the ANOVA analysis of variance procedure to determine whether there was an effect of each treatment. If it is known that there is a significantly different effect from the treatment, it will be continued with the DMRT test at the 5 % level. The results showed that the combination of Bacillus sp. isolate bcz 16 and dosage 35 ml/polybag was the longest delaying the incubation period of 15 day and could suppress moler disease by 50 %. The treatment of Bacillus sp. isolate bcz 16 and dosage 35 ml also gave the highest results in plant length, number of leaves, and wet weight of shallot bulbs
KEMAMPUAN ANTAGONISTIK BEBERAPA ISOLAT Bacillus spp. TERHADAP PATOGEN Pyricularia oryzae PENYEBAB PENYAKIT BLAS TANAMAN PADI (Oryza sativa L.) PADA BEBERAPA KONSENTRASI Setiawan Setiawan; Yenny Wuryandari; Tr Mujoko
Jurnal Ilmu-Ilmu Pertanian Indonesia Vol 27 No 1 (2025)
Publisher : BPFP Universitas Bengkulu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31186/jipi.27.1.67-76

Abstract

Rice (Oryza sativa L.) is a staple food crop that plays a vital role in supporting national food security. One of the major constraints in rice cultivation is blast disease, caused by the fungal pathogen Pyricularia oryzae, which can significantly reduce crop productivity and lead to substantial economic losses. The conventional management of this disease relies heavily on chemical pesticides, which pose long-term risks to environmental and human health. Therefore, eco-friendly alternatives such as biological control agents are necessary, with Bacillus spp. being one promising candidate. This study aimed to evaluate the antagonistic activity of three Bacillus spp. isolates (Bcz-14, Bcz-20, and Bcz-30) against P. oryzae at two bacterial population densities (10⁶ CFU/mL and 10⁹ CFU/mL) under in vitro conditions. The experiment was conducted at the Plant Health Laboratory, Faculty of Agriculture, Universitas Pembangunan Nasional “Veteran” East Java, from January to March 2025. Results of the antagonism assay demonstrated that all Bacillus spp. isolates inhibited the growth of P. oryzae, with the highest inhibition observed in treatments Bcz-14 and Bcz-30 at 10⁶ CFU/mL, with inhibition rates of 24.29% and 23.35%, respectively. The observed inhibition mechanisms included competition and antibiosis. Microscopic observations revealed abnormal hyphal structures in P. oryzae, including lysis, swelling, and chlamydospore formation, in response to Bacillus spp. treatment.  
EFFECT OF GROWING MEDIA ON THE GROWTH OF Streptomyces sp. AND Trichoderma harzianum AS ANTHRACNOSE DISEASE BIOCONTROL Rulinggar, Nia; Mujoko, Tri; Wuryandari, Yenny
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 12 No. 1 (2025)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2025.9257

Abstract

Streptomyces sp. and Trichoderma harzianum is a biological agent that is effective in controlling Colletotrichum capsici, the cause of disease in chili plants. Different growing media can affect the growth and activity of these two microorganisms. This study aims to evaluate the growth of Streptomyces sp. and Trichoderma harzianum on various media, namely rice washing water, coconut water, and sugar potato extract. The research phase includes growing media test, effectiveness test of Streptomyces sp. and Trichoderma harzianum in chili peppers, and effectiveness test of Streptomyces sp. and Trichoderma harzianum on chili seeds. The results showed that the medium of rice washing water is more suitable for Streptomyces sp., coconut water media is more suitable for Trichoderma harzianum, and rice washing water media is more suitable for the combination of the two biological agents. Optimal growth in these media affect the effectiveness of biocontrol in suppressing the severity of anthracnose disease in chili.
Exploration Of Rice Plant Endophyte Bacteria To Suppress Xanthomonas oryzae Bacterial Leaf Blight Disease Putri, Ratih Nadhila; Wuryandari, Yenny; Wahyu, Endang Tri
JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS Vol 11, No 2: Jurnal Pembelajaran Dan Biologi Nukleus June 2025
Publisher : Universitas Labuhanbatu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/jpbn.v11i2.7338

Abstract

Background: Rice is a major food commodity for Indonesian. According to BPS (2022) rice production in 2021 decreased 0.43%. The decline caused by various factors, one of which is bacterial leaf blight disease by the pathogenic bacteria Xanthomonas oryzae. Control of X. oryzae mostly uses pesticides, but this control has a negative impact on the environment, therefore it is necessary to develop endophytic bacteria (EB) that can inhibit the growth of X. oryzae and can act as PGPR. This study was to determine the EB ability in healthy rice stem tissue to suppress bacterial leaf blight disease caused by the bacteria X. oryzae. Methodology: This research was were used isolation from infected plant for X. oryzae and from healthy plant for EB, data was analyzed using the corresponding formula. Findings: found 10 isolates of EB that could inhibit the X. oryzae growth as seen from the inhibition zone produced in the antagonist test. The EB isolates produced the largest inhibition zones such as BE18 (23.3 mm, very strong category), BE16 (13.3 mm, strong category), and BE3 (11.6 mm, strong category). The 4 isolates with bactericidal inhibition mechanisms, namely isolates (BE11, BE15, BE16, and BE18), 6 isolates with bacteriostatic inhibition mechanisms, namely isolates (BE3, BE5, BE6, BE7, BE12, and BE13). Contribution: The availability of EB present in the tissues of healthy rice stems is still relatively high and can be used to suppress the growth of pathogenic bacterium X. oryzae. This rseult also supported by the beneficial properties of EB, which can enhance plant resistance
Antagonist Test of Streptomyces spp. from Shallot Fields in Bojonegoro Against Fusarium sp. Which Causes Moler Disease In vitro Hidayati, Kiki; Mujoko, Tri; Wuryandari, Yenny
BIOEDUSCIENCE Vol 9 No 1 (2025): BIOEDUSCIENCE
Publisher : Universitas Muhammadiyah Prof. Dr. Hamka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22236/jbes/15473

Abstract

Background: Background: Shallots are a horticultural product widely consumed in Indonesia. In 2021, shallot production will decline. This is due to pest and disease attacks on plants. Fusarium sp. is a pathogen that causes the primary disease in shallots, namely moler disease, which can cause losses of up to 50%, so control activities must be carried out. This research aims to determine the potential of Streptomyces sp. bacteria from exploring shallot planting areas in Bojonegoro against Fusarium sp. fungi in vitro. Methods: This study used 15 isolates of Streptomyces sp. from exploration results tested with Fusarium sp. using the dual culture method, and negative control (only Fusarium sp.) repeated 3 times for each treatment. Observations were carried out every day for 7 days by observing the growth of Fusarium sp. hyphae and their inhibitory power. Result: The research showed that isolate S8 had the highest inhibitory power, 49.5%. Meanwhile, the one with the lowest results was isolated S1, 34.5%. From the chitinase test results, the chitinocytic index of isolate S8 was 5.2, which is in the high category. Meanwhile, the isolate S8 cellulolytic index was 4.8 and was classified as high. Conclusions: All Streptomyces sp.p isolates inhibited the growth of Fusarium sp., which was characterized by an inhibition zone compared to the control treatment.
PENGARUH ENKAPSULASI BENIH CABAI RAWIT (Capsicum frutescens L.) DENGAN BERBAGAI BAHAN PEMBAWA PADA BAHAN AKTIF Trichoderma spp. UNTUK PENGENDALIAN PENYAKIT LAYU FUSARIUM Hidayat, Taufik; Nirwanto, Herry; Wuryandari, Yenny
JURNAL AGROTROPIKA Vol. 24 No. 1 (2025): Jurnal Agrotropika Vol 24 No 1, Mei 2025
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/ja.v24i1.8810

Abstract

Fusarium wilt is an important disease of cayenne pepper plants caused by the fungus Fusarium. Trichoderma is a type of biological agent that can be used to control plant diseases, one of which is fusarium wilt. This research was conducted to determine the effect of seed encapsulation with different carrier materials and the active ingredient Trichoderma in controlling fusarium wilt disease. This research was carried out in September 2023-January 2024, at the Plant Health Laboratory and Green House of UPN "Veteran" East Java. This research was prepared using a Completely Randomized Design (CRD) using 7 treatments and 3 replications. The data obtained were analyzed using analysis of variance with a further 5% DMRT test. The research results showed that the use of carrier materials and active ingredients of Trichoderma  influence on ability to control the fusarium wilt of cayenne pepper plants. The resulting inhibitory ability is categorized as capable of controlling fusarium wilt disease with an inhibitory ability of >50% (In vitro) and in vivo can reduce the intensity of the attack to <40%. Carrier materials glutinous flour (in vitro) and compost (in vivo) with the active ingredient Trichoderma harzianum are the best carrier materials in controlling fusarium wilt disease in cayenne pepper plants.
Co-Authors Achmad Fiqri Agus Wahyudi Aisyah Lulu Hariyanto Ajeng Distya Anjani Annisa Zukhruf Oktavia Salamah arfah Arika Purnawati Bambang HADISUTRISNO Bambang Hadisutrisno Budi Rianto Devi Tria Anjarsari Devi Tria Anjarsari Didiek Tranggono Edo Pandu Pratama Erlyana Desy Rahmawati Fadia Nur Rahma Budiarto Frida Nur Aisah Gita Pawana Hera Amalia Putri Belangi Herry Nirwanto HIDAYATI, KIKI I. Hartana Indah Sari Dwi Agustin Indarwati Indarwati Kaswan Badami Kiki Sandra Amelia Kusuma Wardhani Mas’udah Lestari, Safira Rizka Lukmanul Hakim M. Husni Tamrin Maroeto ., Maroeto Maroeto Maroeto Mochammad Mirza Saputra Mochammad Mirza Saputra Muhammad Qoyyimul Amri Munir, Ahmad Arsyad Nauval Zakqy Nirwana Septania Galih Perwira Moekti Nolla Dwi Elvira Noni Rahmadhini Noni Ramadhini Nova Andriani Novita Endah Ariani Novita Endah Ariani Nuriatus Sholikhah Penta SURYAMINARSIH Perwira Moekti, Nirwana Septania Galih Prasetyawati, Endang Triwahyu Putranto Sasikirono Putri, Ratih Nadhila Raharja, Wildan Taufik Ratu Yusnita Zhalsabilla Rulinggar, Nia Safira Rizka Lestari Sagala, Yosua Nathanael Itona Salma Alfiani Nadiva Saputra, Mochammad Mirza Saputra, Mochammad Mirza Setiawan Setiawan Shelomitha Kumala Mawardhany Sherly Arindawati Sri Wiyatiningsih Sulistyanto Suwandi Syamsul Arifin Taufik Hidayat Tr Mujoko Tri MUJOKO Triwidodo ARWIYANTO Triwidodo Arwiyanto Wahyu, Endang Tri Windriyanti, Wiwin Yovita Nathania Yuwono, F. Tsara Nafisah Zainal Abidin Achmad Zakqy, Nauval