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All Journal Jurnal Pengendalian Hayati Agrovigor: Jurnal Agroekoteknologi Plumula : Berkala Ilmiah Agroteknologi Agrifor : Jurnal Ilmu Pertanian dan Kehutanan Jurnal Ilmu-Ilmu Pertanian Indonesia Jurnal Agroekoteknologi Jurnal Perlindungan Tanaman Indonesia JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA BIOEDUSCIENCE Jurnal Teknik Pertanian Lampung (Journal of Agricultural Engineering) JPT : JURNAL PROTEKSI TANAMAN (JOURNAL OF PLANT PROTECTION) JURNAL AGROHITA Jurnal Pembelajaran dan Biologi Nukleus Jurnal Agrotropika PLAKAT : Jurnal Pelayanan Kepada Masyarakat Nusantara Science and Technology Proceedings Berkala Penelitian Hayati Jurnal Pengabdian Kepada Masyarakat JPMP Agrocentrum Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Bioteknologi & Biosains Indonesia
Yenny WURYANDARI
Fakultas Pertanian Universitas Pembangunan Nasional “Veteran” Jawa Timur
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Civil Engineering, Building, Construction & Architecture Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Education Electrical & Electronics Engineering Energy Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Mechanical Engineering Medicine & Pharmacology Social Sciences Other

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Antagonist Test of Streptomyces spp. from Shallot Fields in Bojonegoro Against Fusarium sp. Which Causes Moler Disease In vitro Hidayati, Kiki; Mujoko, Tri; Wuryandari, Yenny
BIOEDUSCIENCE Vol 9 No 1 (2025): BIOEDUSCIENCE
Publisher : Universitas Muhammadiyah Prof. Dr. Hamka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22236/jbes/15473

Abstract

Background: Background: Shallots are a horticultural product widely consumed in Indonesia. In 2021, shallot production will decline. This is due to pest and disease attacks on plants. Fusarium sp. is a pathogen that causes the primary disease in shallots, namely moler disease, which can cause losses of up to 50%, so control activities must be carried out. This research aims to determine the potential of Streptomyces sp. bacteria from exploring shallot planting areas in Bojonegoro against Fusarium sp. fungi in vitro. Methods: This study used 15 isolates of Streptomyces sp. from exploration results tested with Fusarium sp. using the dual culture method, and negative control (only Fusarium sp.) repeated 3 times for each treatment. Observations were carried out every day for 7 days by observing the growth of Fusarium sp. hyphae and their inhibitory power. Result: The research showed that isolate S8 had the highest inhibitory power, 49.5%. Meanwhile, the one with the lowest results was isolated S1, 34.5%. From the chitinase test results, the chitinocytic index of isolate S8 was 5.2, which is in the high category. Meanwhile, the isolate S8 cellulolytic index was 4.8 and was classified as high. Conclusions: All Streptomyces sp.p isolates inhibited the growth of Fusarium sp., which was characterized by an inhibition zone compared to the control treatment.
PENGARUH ENKAPSULASI BENIH CABAI RAWIT (Capsicum frutescens L.) DENGAN BERBAGAI BAHAN PEMBAWA PADA BAHAN AKTIF Trichoderma spp. UNTUK PENGENDALIAN PENYAKIT LAYU FUSARIUM Hidayat, Taufik; Nirwanto, Herry; Wuryandari, Yenny
JURNAL AGROTROPIKA Vol. 24 No. 1 (2025): Jurnal Agrotropika Vol 24 No 1, Mei 2025
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/ja.v24i1.8810

Abstract

Fusarium wilt is an important disease of cayenne pepper plants caused by the fungus Fusarium. Trichoderma is a type of biological agent that can be used to control plant diseases, one of which is fusarium wilt. This research was conducted to determine the effect of seed encapsulation with different carrier materials and the active ingredient Trichoderma in controlling fusarium wilt disease. This research was carried out in September 2023-January 2024, at the Plant Health Laboratory and Green House of UPN "Veteran" East Java. This research was prepared using a Completely Randomized Design (CRD) using 7 treatments and 3 replications. The data obtained were analyzed using analysis of variance with a further 5% DMRT test. The research results showed that the use of carrier materials and active ingredients of Trichoderma  influence on ability to control the fusarium wilt of cayenne pepper plants. The resulting inhibitory ability is categorized as capable of controlling fusarium wilt disease with an inhibitory ability of >50% (In vitro) and in vivo can reduce the intensity of the attack to <40%. Carrier materials glutinous flour (in vitro) and compost (in vivo) with the active ingredient Trichoderma harzianum are the best carrier materials in controlling fusarium wilt disease in cayenne pepper plants.
Optimization of Bioencapsulation of Bacillus sp. to Increase Resistance to Bacterial Wilt and Growth of Cayenne Pepper Anjani, Ajeng Distya; Wuryandari, Yenny; Lestari, Safira Rizka
Jurnal Teknik Pertanian Lampung (Journal of Agricultural Engineering) Vol. 14 No. 4 (2025): August 2025
Publisher : The University of Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jtepl.v14i4.1325-1334

Abstract

Cayenne pepper has a high production demand. The high demand does not match the amount of production produced. One of the factors for decreased production is the presence of bacterial wilt disease caused by Ralstonia solanacearum. Bacterial wilt control can be done with the application of antagonistic bacteria, namely Bacillus sp. Bacillus sp. application requires an optimal formulation to maintain bacterial activity in the soil, one of which is Bacillus sp. encapsulation. This study aims to determine the effectiveness of Bacillus sp. encapsulation as a formulation of biological agent bacteria for the control of bacterial wilt disease and cayenne pepper plant growth. This study used the encapsulating material concentration and dosage factors for application to plants. Encapsulation was made using the extrusion method. The results showed that the concentration of 2% sodium alginate and 1.5% gelatin gave a significant difference in disease intensity of 18.89% and in the growth parameters of cayenne pepper plants were able to reach a height of 30.58 cm and a root length of 17.18 cm. The right concentration and high dose provide the ability to suppress intensity and encourage maximum growth
Eksplorasi dan Uji Antagonis Bakteri Endofit Dari Daun Tanaman Mangrove Avicennia sp. Terhadap Jamur Patogen Alteria sp. Secara In Vitro Perwira Moekti, Nirwana Septania Galih; Prasetyawati, Endang Triwahyu; Wuryandari, Yenny
Jurnal Agroekoteknologi Vol 17, No 1 (2025)
Publisher : Jurusan Agroekoteknologi Fakultas Pertanian Untirta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33512/jur.agroekotetek.v17i1.30972

Abstract

Mangrove (Avicennia sp.) merupakan salah satu ekosistem penting yang sering ditemukan di muara sungai yang memiliki kemampuan untuk tumbuh dan berkembang di zona salinitas tinggi. Salah satu jenis tanaman mangrove yang bermanfaat dan terdapat berbagai jenis bakteri endofit adalah Avicennia marina. Oleh karena itu, penelitian ini bertujuan untuk mendapatkan isolat bakteri endofit yang berpotensi sebagai agensia hayati pada daun tanaman bakau Avicennia sp. terhadap cendawan patogen Alternaria sp. Metode isolasi yang digunakan adalah penanaman langsung. Pengujian antagonis dilakukan dengan metode dual culture melalui pengukuran diameter zona hambat. Sampel yang diambil adalah daun yang sehat dan segar. Bakteri endofit yang diperoleh sebanyak 24 isolat bakteri yang memiliki morfologi yang berbeda. Diameter zona hambat dengan kategori kuat adalah isolat D28 sebesar 12,5 mm dengan kategori kuat dan kategori sedang adalah isolat D13, D19 sebesar 7,5 mm; 8,5 mm.  Mekanisme kerja bakteri endofit adalah antibiosis dan kompetisi ruang. Hasil penelitian ini dapat disimpulkan bahwa terdapat bakteri endofit dari daun mangrove Avicennia sp. yang berpotensi sebagai antijamur terhadap jamur patogen Alternaria sp.
Screening and Potential Testing of Metarhizium sp. Isolates for Controlling Lepidiota stigma (Fabricius) Larvae in Sugarcane (Saccharum officinarum L.) Sulistyawati, Hanik; Windriyanti, Wiwin; Wuryandari, Yenny
Jurnal Teknik Pertanian Lampung (Journal of Agricultural Engineering) Vol. 14 No. 5 (2025): October 2025
Publisher : The University of Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jtepl.v14i5.1714-1725

Abstract

Lepidiota stigma is a major pest contributing to the decline in sugarcane quality in East Java, necessitating effective control measures that support sustainable agriculture. Metarhizium anisopliae is an entomopathogenic fungus that acts as a natural enemy of L. stigma and is expected to control its infestations. This study aims to evaluate the potential of isolated strains in killing the larvae and to identify the compounds produced by Metarhizium spp. The research involved exploration, morphological identification, virulence testing, and compound analysis. Isolate screening was conducted in two stages: the first stage involved screening 20 isolates down to 5 potential ones, and the second stage assessed the characteristics and effectiveness of these 5 isolates in controlling L. stigma larvae at spore concentrations of 10⁶, 10⁷, and 10⁸ spores/ml in vitro. The parameters observed were larval mortality and metabolite content, analyzed using LC-HRMS (Liquid Chromatography High-Resolution Mass Spectrometry). Metarhizium spp. isolated from soil exploration showed an effect on L. stigma mortality. The fungus produced secondary metabolites, including destruxins A4, E, and Ed1, which contributed to the mortality of L. stigma larvae.
Potensi Konsorsium Bakteri Pseudomonad fluorescent dan Bacillus mycoides Terhadap Penyakit Layu Bakteri Secara In-vitro Sagala, Yosua Nathanael Itona; Prasetyawati, Endang Triwahyu; Wuryandari, Yenny
Jurnal Proteksi Tanaman (Journal of Plant Protection) Vol. 8 No. 2 (2024): December 2024
Publisher : Plant Protection Department, Faculty of Agriculture, Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jpt.8.2.78-87.2024

Abstract

Bacterial wilt disease caused by Ralstonia solanacearum is a disease in chili pepper plants (Capsicum annuum) that is difficult to control. One technique to prevent bacterial wilt disease is consortium of antagonistic bacteria such as Pseudomonad fluorescent and Bacillus mycoides. This study aimed to determine whether the consortium of Pseudomonad fluorescent pf-142 and Bacillus mycoides can inhibit R. solanacearum better than a single application in-vitro. This study was conducted in a Completely Randomized Design (CRD) with four treatments (Pseudomonad fluorescent pf-142, B. mycoides, Pseudomonad fluorescent pf-142 + B. mycoides, and control) in six replications, resulting in 24 experimental units. The variables observed were the symptoms of attack and pathogenicity of R. solanacearum, compatibility and inhibitory rate of the consortium of Pseudomonad fluorescens pf-142 and B. mycoides against R. solanacearum. Based on the study, it was known that R. solanacearum caused wilting in chili pepper plants with high virulence. Pseudomonad fluorescent pf-142 and B. mycoides did not produce an inhibition zone, indicating that both were compatible. The consortium of Pseudomonad fluorescent pf-142 and B. mycoides provided the widest inhibition zone, indicating strong antagonistic ability against R. solanacearum.
EVALUATING THE LONG-TERM STORAGE TIME VIABILITY AND SIZE DYNAMICS OF BACILLUS SP. BIOENCAPSULATION IN SODIUM ALGINATE MATRIX Saputra, Mochammad Mirza; Wuryandari, Yenny; Rahmadhini, Noni; Lestari, Safira Rizka
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 10 No. 2 (2023)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2023.2549

Abstract

The use of biological agents such as Bacillus sp. bacteria has begun to be widely used by farmers as a new form of control. Bacillus sp. needs to require special methods in its application because it has certain living conditions, and Bacillus sp. is vulnerable to environmental pressures. Bioencapsulated formulation in the form of granules is considered effective as a form of bacterial application in soil because it is able to protect Bacillus sp. and maintain survival. This study aims to determine the best concentration of sodium alginate in maintaining the viability of Bacillus sp. at the in vitro stage and to see changes in the size of the beads. The beads were made using extrusion method by combining Bacillus sp. suspension and sodium alginate suspension at 1%, 1.5%, and 2% concentration. The concentration of sodium alginate was not a major factor in changes in bead size and viability test results experienced significant changes in each observation. 1.5% and 2% concentrations can reach the highest viability value of 3x106 CFU/mL and decreased during 1 month of observation. Changes in bead size and viability were caused by the alginase enzyme produced by Bacillus sp.
EFFECT OF GROWING MEDIA ON THE GROWTH OF Streptomyces sp. AND Trichoderma harzianum AS ANTHRACNOSE DISEASE BIOCONTROL Rulinggar, Nia; Mujoko, Tri; Wuryandari, Yenny
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 12 No. 1 (2025)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2025.9257

Abstract

Streptomyces sp. and Trichoderma harzianum is a biological agent that is effective in controlling Colletotrichum capsici, the cause of disease in chili plants. Different growing media can affect the growth and activity of these two microorganisms. This study aims to evaluate the growth of Streptomyces sp. and Trichoderma harzianum on various media, namely rice washing water, coconut water, and sugar potato extract. The research phase includes growing media test, effectiveness test of Streptomyces sp. and Trichoderma harzianum in chili peppers, and effectiveness test of Streptomyces sp. and Trichoderma harzianum on chili seeds. The results showed that the medium of rice washing water is more suitable for Streptomyces sp., coconut water media is more suitable for Trichoderma harzianum, and rice washing water media is more suitable for the combination of the two biological agents. Optimal growth in these media affect the effectiveness of biocontrol in suppressing the severity of anthracnose disease in chili.
Co-Authors Achmad Fiqri Agus Wahyudi Aisyah Lulu Hariyanto Anjani, Ajeng Distya arfah Arika Purnawati Bambang HADISUTRISNO Bambang Hadisutrisno Budi Rianto Devi Tria Anjarsari Devi Tria Anjarsari Didiek Tranggono Edo Pandu Pratama Fadia Nur Rahma Budiarto Frida Nur Aisah Gita Pawana Hera Amalia Putri Belangi Herry Nirwanto HIDAYATI, KIKI I. Hartana Indah Sari Dwi Agustin Indarwati Indarwati Kaswan Badami Kiki Sandra Amelia Kusuma Wardhani Mas'udah Lestari, Safira Rizka M. Husni Tamrin, M. Husni Maroeto ., Maroeto Maroeto Maroeto Mas’udah, Kusuma Wardhani Mochammad Mirza Saputra Munir, Ahmad Arsyad Nauval Zakqy Noni Rahmadhini Noni Rahmadhini Nova Andriani Novita Endah Ariani Nuriatus Sholikhah Penta SURYAMINARSIH Perwira Moekti, Nirwana Septania Galih Prasetyawati, Endang Triwahyu Putranto Sasikirono Putri, Ratih Nadhila Raharja, Wildan Taufik Rahmadhini, Noni Ratu Yusnita Zhalsabilla Rulinggar, Nia Safira Rizka Lestari Safira Rizka Lestari Sagala, Yosua Nathanael Itona Saputra, Mochammad Mirza Saputra, Mochammad Mirza Setiawan Setiawan Shelomitha Kumala Mawardhany Sherly Arindawati Sri Wiyatiningsih Sulistyanto Sulistyawati, Hanik Suwandi Syamsul Arifin Taufik Hidayat Tr Mujoko Tri MUJOKO Triwidodo ARWIYANTO Triwidodo Arwiyanto Wahyu, Endang Tri Windriyanti, Wiwin Yovita Nathania Yuwono, F. Tsara Nafisah Zainal Abidin Achmad Zakqy, Nauval