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Development of E-Module Based on Contextual Teaching and Learning on Human Mobility Material to Improve Science Literacy Abilities of Class XI Students Jannah, Lu'lu'atul; Utami, Nur Rahayu
Journal of Biology Education Vol. 13 No. 2 (2024): Agustus 2024
Publisher : Universitas Negeri Semarang

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Abstract

The latest PISA score results in 2022 state that the scientific literacy abilities of students in Indonesia are in the low category. Based on the results of a questionnaire analyzing the needs for teaching materials at SMA Teuku Umar Semarang, the teaching materials implemented had not yet trained scientific literacy in the aspects of content, process and context. The aim of this research is to develop an electronic module (E-module) based on Contextual Teaching and Learning (CTL) on human mobility material that is feasible, practical and effective to improve students' scientific literacy skills. The research design used is Research and Development using the 4D (Four-D) model stages, including: Define, Design, Development, and Dissemination. Data collection tools include product feasibility questionnaires, product practicality questionnaires, and multiple choice test instruments. Based on the research results, the e-module was declared very feasible with a percentage score of 96.2% by material experts and 97.9% by media experts. In the practicality test, it was stated that it was very practical with the percentage of scores obtained based on teacher responses, namely 87% and the percentage of scores from students of 89%. In the effectiveness test, it was declared effective in improving scientific literacy skills with a percentage of 94.83% of students who obtained N-Gain scores in the medium and high categories.
CHARACTERIZATION OF MICROBIAL DIVERSITY AND FATTY ACID COMPOSITION IN THE INTESTINE OF HEALTHY DOMESTIC DUCKS : MICROBIAL COMMUNITY AND FATTY ACIDS PROFILE IN DUCKS INTESTINE Susanti, R; Rahayu Utami, Nur; Dafip, Muchamad
BIOTROPIA Vol. 33 No. 1 (2026): BIOTROPIA Vol. 33 No. 1 January 2026
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11598/btb.2026.33.1.2451

Abstract

ARTICLE HIGLIGHTS- Healthy duck gut shows rich bacterial diversity dominated by Firmicutes.- Duck intestine is enriched in saturated fatty acids compared to the feed.- Short chain fatty acids in duck intestine greatly exceed those in the feed.- Genera Lactobacillus and Ruminococcus are key markers of duck gut health.- Palmitic, stearic, and vaccenic acids emerge as major intestinal lipids.ABSTRACTFatty acids exhibit antibacterial properties and serve as metabolic substrates for intestinal bacteria, shaping the composition of the microbiota and the synthesis of microbial metabolites. This study aimed to analyze the duck intestinal microbiota community and its fatty acid profile. This study utilized 25 healthy domestic ducks (Anas platyrhynchos) sourced from an intensive laying farm in Central Java, Indonesia. An exploratory observational research design was employed to analyze data obtained from this study. The intestinal bacteria of duck samples were analyzed using Next-Generation Sequencing (NGS), and fatty acid components using Gas Chromatography–Mass Spectrometry (GC-MS). Duck feed samples were analyzed for nutritional composition using proximate analysis and fatty acid composition using GC-MS. The Simpson and Shannon indices revealed rich microbial diversity in the duck intestine. The abundance of bacteria was dominated by several phyla, such as Firmicutes (82.58%), Actinobacteria (12.25%), and Bacteroidetes (4.27%). The Firmicutes/Bacteroidetes ratio was 19.32, with the intestine containing greater proportion of saturated fatty acids (74.32%) compared to unsaturated fatty acids (33.62%). Additionally, the concentration of short-chain fatty acids (SCFAs) in the duck’s intestine was 15.72 times greater than that found in the feed. Microorganisms, including Bacteroides (2.88%), Faecalibacterium (0.16%), Prevotella (0.08%), Butyricicoccus (0.67%), Ruminococcus (2.2%), Oscillospira (0.02%), and Mucisspirillum (0.018%) are genera that produce SCFA. Bacteria capable of synthesizing medium-chain fatty acids (MCFA) were identified, namely Ruminococcaceae UGC-005 (1.85%) and Acinetobacter (0.005%). Bacteria producing saturated long-chain fatty acids (LCFA) were identified, namely Lactobacillus (12.12%), Prevotella (0.08%), and Alistipes (0.13%). Bacteria capable of producing polyunsaturated fatty acids (PUFA) metabolites including conjugated linoleic acid (CLA) and conjugated linolenic acid (CLnA) were identified, namely Propionibacterium (0.004%), Lactobacillus (12.12%), and Bifidobacterium (0.21%). In summary, the intestinal microbiota of healthy ducks exhibits considerable diversity and a high diversity index. Various bacterial genera contribute to the production of SCFA, MCFA, and LCFA, as well as the conversion of PUFA into CLA and CLnA metabolites. This baseline microbiota–fatty acid profile can guide microbiota-informed feed optimization and candidate probiotic selection to support SCFA production, gut health, and productivity, and it provides a reference for monitoring dysbiosis and evaluating dietary interventions in intensive duck production systems.
Co-Authors Aditya Marianti Alaina Mawadah, Alaina Alighiri, Dante Aminuyati Anang Ma'ruf Setyawan Andin Irsadi Andreas Priyono Budi Prasetyo Anifatussholihah, Anifatussholihah Anifatussholihah, Anifatussholihah Ari Yuniastuti Arif Rahman Hakim Arif Widiyatmoko, Arif Arya Iswara Aulia Nur Lita, Aulia Nur Basyuri, Ade Dafip, Muchamad Desinta Dwi Nuriyanti, Desinta Dwi Dewi Mustikaningtyas Dhyaksa, R. Nana Anindita Eka Novita Sari, Eka Novita Eling Purwantoyo Ely Rudyatmi, Ely Endah Peniati Enni Suwarsi Evin Rusdiana Sari, Evin Rusdiana Fidia Fibriana Fitriani, Kasih Fitriani, Kasih Ika - Mustikasari, Ika - Irfan Ihza Wardana Irma Paramita, Irma Jannah, Lu'lu'atul KARTIKA WIDYANINGRUM Kukuh Santosa Kukuh Santosa Kurniahtunnisa Kurniahtunnisa Kusuma, Zeni Herawati Layyinatus Syifa, Liftia Lina Herlina Lisdiana Lisdiana Margareta Rahayuningsih Margareta Rahayuningsih Nugroho Edi K Martien Herna Susanti Milatina Murni Lestari Mulyaningsih, Erlina Muspita Dewi, Muspita Mustika Sari, Mustika Mu’afy, Faizal Daffa Nana Kariada Trimartuti Nazlafatin Hamida Ngudy Jaka Surasa, Ngudy Jaka Niken Subekti Nugrahaningsih WH Nur Kusuma Dewi Nur Zubaidah, Nur Nurfa Anung Anidityas, Nurfa Anung Oky Ristya Trisnawati, Oky Ristya Pramasta, Bagas Priyantini Widiyaningrum Rahayuningtiyas, Astri Rahmatika, Atik Yusni Rahmawati, Nur Fitria Rosidah Ruqoyah, Umu Saiful Ridlo Selvi Marcellia Setyo Yuwono Siti Alimah Siti Alimah Siti Nurmar Laela Sri Sukaesih Sriyadi , Sriyadi Stephani Diah Pamelasari, Stephani Diah Supriyanto - Supriyanto - -, Supriyanto - SUWARTI Titik Widayanti, Titik Tyas Agung Pribadi, Tyas Agung Una Lailis Tsani, Una Lailis Utari Ani Susanah Utari Ani Susanah, Utari Ani Wahyuningtyas, Arini Tri Wandah Wibawanto Wardani, Destia Ayu Kusuma WIDOWATI Wiwi Isnaeni Wulan Christijanti Zulfa Wafda, Zulfa