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Protein Kasar Plasma Seminalis Sapi Menurunkan Kejadian Nekrosis Spermatozoa Kambing yang Disimpan pada Suhu Dingin (BULL SEMINAL PLASMACRUDE PROTEIN DECREASING THE PERCENTAGE OF NECROSISIN GOATSPERMATOZOA THAT KEPT IN COLD TEMPARATURE) Suherni Susilowati; Hardijanto .; Indah Norma Triana
Jurnal Veteriner Vol 17 No 1 (2016)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (96.169 KB)

Abstract

The aim of this study was to determine the benefits of bullseminal plasma crude proteinin goatspermatozoa diluent to improve of frozen semen quality.This study consists of three treatments: (i)treatmentcontrol (P0,goat semen + diluents ; (ii) treatment I(PI, diluent + goat semen + bull seminal plasmacrudeprotein (1:1);and (iii)treatment II (PII, diluent + goat semen+ bull seminal plasma crude protein (1:2). Alltreatments were kept in arefrigerator (5oC) andobserved for necrosis of goatspermatozoa, daily. The highestspermatozoa necrosis was observed in the diluter without the bull seminal plasma crude protein eitheratday1, 2, 3, 4orday 5. The necrosis was statistically significant different (p<0,05) between P0, PI and PII.withthe lowest percentagenecrosis at PI. In conclusion, the addition of bull seminal plasma crude proteinin skim milk diluentwill decrease theincidenceof necrosis ingoat spermatozoa which were kept in coldtemperature.
PROGRAM PENGGEMUKAN TERNAK DOMBA EKOR GEMUK DI KECAMATAN SEMANDING, KABUPATEN TUBAN Indah Norma Triana; Rr. Ratih Ratnasari; Ajik Azmijah
Jurnal Layanan Masyarakat (Journal of Public Services) Vol. 1 No. 2 (2017): Jurnal Layanan Masyarakat
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (775.621 KB) | DOI: 10.20473/jlm.v1i2.2017.51-55

Abstract

The program for fattening and breeding of fat-tailed sheep (EG) in Semanding District, Tuban Regency, was collaboration work among Institute for Research and Community Service (LPPM) of Universitas Airlangga, Gemah Ripah, and Mandiri’s Breeding Coomunity, Tuban Regency. The program was conducted by veterinarians, who are experts in animal reproduction, animal feeding and health, camping and its sanitation, assisted by local people. The activities included training on livestock breeding, demonstration of animal feeding and caging, vaccination, livestock maintenance at pre- and post-production, and also the health examination and treatment of sick fat-tailed sheep. There was also presentation about medicines used for animal fattening, i.e. Bio-N-Plus, Premix Minerals, and vitamin B-Complex. These drugs were given to the participants to accelerate the cattle growth. In addition, a simulation on how to breed fat-tailed sheep was also demonstrated to the participants. At the end of the program, a package was given to Gemah Ripah dan Mandiri’s Breeding Community. It consisted of six fat-tailed sheep, 3 quintals of concentrate feeds used for about four months feeding, the money for renovating the animal cages, vitamins, medicines, and vaccines. This training program was successfully increased the ability of participants, Gemah Ripah and Mandiri’s Breeding Coomunity, to breed the fat-tailed sheep in a relatively good way. It was supported by the facts that the cattle had increased body weights and there was no observed risk of sickness and animal death. AbstrakProgram penggemukan dan budidaya ternak domba ekor gemuk (EG) di Kecamatan Semanding, Kabupaten Tuban merupakan bentuk kerja sama antara Lembaga Pengabdian Kepada Masyarakat Universitas Airlangga dengan Kelompok Ternak Gemah Ripah dan Mandiri, Tuban. Pelaksanaan program ini melibatkan dokter hewan, yang merupakan ahli reproduksi, ahli pakan ternak, ahli kesehatan ternak serta ahli sanitasi dan perkandangan, serta dibantu oleh tenaga lokal. Kegiatan yang dilakukan antara lain meliputi: pelatihan budidaya ternak domba EG, peragaan pembuatan pakan dan kandang, pemberian vaksin, pemeliharaan pra- dan pasca- produksi, serta pemeriksaan dan pengobatan domba yang sakit. Pada kesempatan ini, juga diperkenalkan obat untuk penggemukan domba, yaitu: Bio-N-Plus, Premix Mineral, dan vitamin B-Kompleks. Bahan tersebut diberikan dengan tujuan untuk mempercepat pertumbuhan domba. Selain itu, juga dilakukan simulasi beternak domba EG bagi para peternak. Pada akhir pelatihan telah diberikan paket ternak domba EG kepada kelompok peternak Gemah Ripah Dan Mandiri, yaitu berupa: 6 ekor domba EG, 3 kwintal pakan konsentrat yang bisa digunakan untuk empat bulan, biaya renovasi kandang domba EG, serta vitamin, obat-obatan, dan vaksin. Dari hasil pelaksanaan program ini, para peternak telah berhasil memelihara domba EG dengan cara yang lebih baik dengan tanda pertumbuhan berat badan ternak yang semakin meningkat, serta tidak ada resiko sakit dan kematian hewan ternak.
POTENSI ANTIBODI SPERMATOZOA TERHADAP SPERMATOGENESIS DAN FERTILISASI PADA TIKUS PUTIH Indah Norma Triana
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 12 No 2 (2007): June 2007
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/352

Abstract

The experiment was conducted the potential of antiibody sperm to spermatogenesis and fertility of the male rat. Ten of rat testis have been used in this research, then crushed and added with 10 cc saline and then were filtrated and centrifuged 1800 rpm for ten minutes. Twenty four of the male rat divided in to four groups. Group I: injected with saline 0.1 cc/sc/day for 45 days; group II: injected with testis suspension 0.1 cc/sc/day for 15 days; group III: injected with testis suspension 0.1 cc/sc/days for 30 days; group IV: injected with testis suspension 0.1 cc /sc/days for 45 days. Then each of the male rat were mated with female, then the number of the total of pups were counted. Male rat were killed and testis were collected for preparation of histology and count of the spermatocyte and spermatid. The results show that the number of the spermatocyte and spermatid has significant different in the group and control (p < 0.05). The number of the pups significantly different in the group and control (p < 0.05). Implication of this research was injected Antibodisperm on male rat can decrease of the number of spermatocyte, spermatid and the number of the pups.
PENGARUH WAKTU INSEMINASI TERHADAP MOTILITAS DAN VIABILITAS SPERMATOZOA PASCAINSEMINASI PADA KAMBING Indah Norma Triana
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 11 No 2 (2006): June 2006
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/385

Abstract

The purpose of this research was to know the effect of insemination time on motility and viability of spermatozoa in egg yolk tris diluter post insemination of goat. In this research 20 female goats was used and divided into two groups and synchronized with PGF2 alfa. If sign of oestrus appeared, then goats in group I, were inseminated with semen from buck diluted with egg yolk tris at the beginning of oestrus and group II inseminated with semen from buck diluted with egg yolk tris at the mid oestrus. Semen was collected from cervical canal of goats at one, two, 3, 6, or 24 hours after insemination for evaluating its motility and viability. Implication of this research is indicate that artificial insemination can be conducted in the early also the mid oestrus of goat.
The Effect of Soybean FlourFormulationDietary on Cecal Mucosa and the Number of Goblet Cells of Coccidia Infected Broiler Anton Sulistiono; Lucia Tri Suwanti; Hana Eliyani; Indah Norma Triana
Journal of Parasite Science (JoPS) Vol. 1 No. 1 (2017): Journal of Parasite Science
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (818.508 KB) | DOI: 10.20473/jops.v1i1.16054

Abstract

Tujuan dari penelitian ini adalah untuk mengetahui pengaruh tepung kedelai dalam formulasi makanan terhadap cedera caecum dan perubahan jumlah sel piala yang terpajan koksidiosis caecal broiler. Dua puluh ayam broiler pada umur 15 hari diacak menjadi 4 kelompok perlakuan. P0 diberi makan basal dan tidak terinfeksi E. tenella . P1 diberi makan basal ditambahkan dengan tepung kedelai 35% dan tidak terinfeksi E. tenella. P2 diberi makan basal dan terinfeksi. Basal yang diberi makan P3 menambahkan 35% tepung kedelai dan terinfeksi. Data yang diperoleh dari skor lession dianalisis dengan uji Kruskall Wallis, sedangkan data jumlah sel piala dianalisis dengan ANOVA. Hasil penelitian menunjukkan bahwa tepung kedelai mengurangi skor lesi cecal (p> 0,05) dan tidak berpengaruh pada peningkatan jumlah sel cecal goblet (p> 0,05) ayam broiler yang terinfeksi coccidia.
The Prevalance and Helminth Infection Degree of Gastrointestinal in Layer Duck Located in Keper and Markolak Kramat Village District of Bangkalan Regency of Bangkalan Ana Amaliah; Indah Norma Triana; Poedji Hastutiek; Setiawan Koesdarto; Lucia Tri Suwanti; Soeharsono Soeharsono
Journal of Parasite Science (JoPS) Vol. 2 No. 1 (2018): Journal of Parasite Science
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (423.067 KB) | DOI: 10.20473/jops.v2i1.16376

Abstract

Tujuan dari penelitian ini adalah untuk menentukan prevalensi dan derajat infeksi cacing saluran pencernaan pada itik petelur di dua kondisi lingkungan yang berbeda. Sampel feses diperiksa dengan metode sedimentasi dan metode apung kemudian dilanjutkan dengan menghitung telur cacing per gram tinja dengan metode Mc Master. Hasil menunjukkan bahwa 22,9% (8/35) itik petelur di Dusun Keper terinfeksi oleh Capillaria sp., dan Echinostoma revolutum, dengan rata-rata derajat infeksi sebsar 52,50 ± 41,662, sedangkan 31,4% (11/35) itik petelur di Dusun Markolak terinfeksi oleh Capillaria sp., Echinostoma revolutum, dan cacing dari kelas cestoda, dengan rata-rata derajat infeksi sebesar 155,45 ± 166,395. Infeksi bersifat tunggal maupun campuran. Analisis terhadap prevalensi dan derajat infeksi cacing saluran pencernaan itik petelur menunujukkan hasil yang tidak signifikan (p>0,05) antara Dusun Keper dan Dusun Markolak.
KEJADIAN KAWIN BERULANG PADA SAPI POTONG BETINA PERANAKAN LIMOSIN DAN SIMENTAL DI KECAMATAN WONOSALAM KABUPATEN JOMBANG PERIODE 2015 - 2018 Subagio, Muharam; Triana, Indah Norma; Poernomo, Bambang; Wurlina, Wurlina; Srianto, Pudji; Utomo, Budi
Journal of Basic Medical Veterinary Vol. 8 No. 2 (2019): Journal of Basic Medical Veterinary, December 2019
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/.v8i2.20412

Abstract

Repeat Breeding is an adult cattle which have normal estrous cycle and clear signs of estrous but did not get pregnant when it got breed by natural mating or artificial insemination. The main purposes of this study is to know the number of repeat breeding cases in beef cattle in regency Jombang period of 2015-2018. The metode of the study is survey which collected by recording and survey. Were analyzed though Normality Test (Kolmogorov-Smirnov Test) and Homogenity Test and followed by T Test. Questionnaire test result processed using Kendall's Tau-b Correlation Test. Study result showed that repeat breeding cases fluctuate significantly per year and the factors affecting fertilization failure and early embrionic death, hormonal, infection, and management factors.   
PENGARUH PEMBERIAN EKSTRAK KULIT SEMANGKA (Citrullus lanatus) TERHADAP MOTILITAS DAN VIABILITAS SPERMATOZOA TIKUS (Rattus norvegicus) DENGAN PAPARAN SUHU PANAS Rohmah, Lailatul; Triana, Indah Norma; Sunarso, Agus; Susilowati, Suherni; Hidajati, Nove; Kurnijasanti, Rochmah
Ovozoa: Journal of Animal Reproduction Vol. 7 No. 2 (2018): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (484.302 KB) | DOI: 10.20473/ovz.v7i2.2018.131-136

Abstract

High temperatures lead to oxidative stress which can decrease the quality of spermatozoa. Watermelon's rind have an ability for antioxidants effect that can reduce sperm's damages that results by role explanation of free radicals. This study aimed to determine the effect of extract of watermelon's rind on the motility and viability of sperm on male rat exposed to heat. This experiments use rat as animals experiment with five treatments and four repetitions. Five treatments are: P0: without exposured to 40ËšC of temperature and without treatment by extract watermelon's rind, P1: was exposured to 40ËšC of temperature and without treatment by extract watermelon's rind, P2: was exposured to 40ËšC of temperature and treatments by extract watermelon's rind dosage 20 mg/rat/day, P3: was exposured to 40ËšC of temperature and treatments by extract watermelon's rind dosage 40 mg/rat/day, and P4: was exposured to 40ËšC of temperature and treatments by extract watermelon's rind dosage 80 mg/rat/day. The treatment was done for 52 days. The results, P4 have the best effect that can be increase the motility and viability of rat's sperm (Rattus norvegicus) exposed to heat.
PERANAN VITAMIN B12 DALAM PENGENCER SUSU SKIM KUNING TELUR TERHADAP MOTILITAS DAN VIABILITAS SPERMATOZOA DOMBA SAPUDIPOST THAWING Arini, Mirza Asafaizh; Suprayogi, Tri Wahyu; Warsito, Sunaryo Hadi; Sardjito, Trilas; Susilowati, Suherni; Triana, Indah Norma
Ovozoa: Journal of Animal Reproduction Vol. 8 No. 2 (2019): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (348.668 KB) | DOI: 10.20473/ovz.v8i2.2019.110-115

Abstract

The purpose of this study was to prove that the addition of Vitamin B12 in egg yolk skim milk extender can maintain the motility and viability of post thawing Sapudi sheep spermatozoa. This study used sample of Sapudi sheep fresh semen that was collected with artificial vagina, and divided into 3 different treatments. The control treatment or P0, Sapudi sheep semen was diluted in the egg yolk skim milk extender + vitamin C 0,02 mg / ml, treatment group (P1 and P2), Sapudi sheep semen was diluted in the egg yolk skim milk extender+ vitamin B12 5 μg / ml and grouped in P1, Sapudi sheep semen was diluted in the egg yolk skim milk extender + Vitamin C 0.02 mg / ml + Vitamin B12 5μg / ml and grouped in P2. The data analysis was use One Way Analysis of Variant (ANOVA), then to identify the real difference among the treatments continued with Duncan distance test. The result of data analysis showed that P2 treatment was significantly different (p <0.05) with P1 treatment and P0 treatment or control. While P1 treatment was not significantly different (p> 0,05) with P0 treatment or control. The result showed that the addition of vitamin C 0.02 mg / ml with vitamin B12 5 μg / ml combination in egg yolk skim milk extender was the best addition to maintain the motility and viability of the post thawing Sapudi sheep spermatozoa.
PENGARUH PENGENCER KUNING TELUR AYAM DENGAN AIR KELAPA MUDA TERHADAP INTEGRITAS MEMBRAN PLASMA DAN ABNORMALITAS SPERMATOZOA DOMBA SAPUDI PENGARUH PENGENCER KUNING TELUR AYAM DENGAN AIR KELAPA MUDA TERHADAP INTEGRITAS MEMBRAN PLASMA DAN ABNORMALITAS SPERMATOZOA DOMBA SAPUDI Cesaria, Mayrena Ayu; Estoepangestie, A.T.Soelih; Susilowati, Suherni; Hernawati, Tatik; Madyawati, Sri Pantja; Triana, Indah Norma
Ovozoa: Journal of Animal Reproduction Vol. 8 No. 2 (2019): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (341.809 KB) | DOI: 10.20473/ovz.v8i2.2019.139-143

Abstract

This study aimed to determine the influence of mixture diluter of chicken egg yolk and young coconut water on the integrity of plasma membrane and abnormalities of Sapudi sheep spermatozoa. The experimental design used in this study was a complete random method. The data was analysed using ANOVA, and if there is a significant difference then proceed with Duncan's Multiple Range Test ( DMRT ) at significance level of 5%. Based on the result of the research, the influence of mixture diluter of chicken egg yolk and young coconut water was not significantly different ( p > 0,05 ) on the intact plasma membrane of spermatozoa on the first day until the fourth day. Interaction between treatment and storage duration had no effect on spermatozoa abnormality on the first day, second day and the fourth day. It can be concluded that mixture diluter of chicken egg yolk and young coconut water had a good effect on the plasma membrane and spermatozoa abnormalities up to the third day.