Article Per Year (5 Year)
p-Index From 2021 - 2026
0.444
P-Index
This Author published in this journals
All Journal Jurnal Sain Veteriner Jurnal Veteriner Journal of Parasite Science Ovozoa: Journal of Animal Reproduction Jurnal Kedokteran Hewan
Abdul Samik
Departemen Reproduksi Veteriner Fakultas Kedokteran Hewan Universitas Airlangga Surabaya
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Veterinary

Published : 7 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 2 Documents
Search
Journal : Jurnal Veteriner

 1 
Aluminosilikat Berpotensi Menekan Gangguan Reproduksi Mikotoksin Zearalenon Berdasarkan Pengamatan Jumlah Folikel dan Ekspresi Caspase-9 Ovarium Muhammad Thohawi Elziyad Purnama; Imam Mustofa; Tri Wahyu Suprayogi; Abdul Samik; Ragil Angga Prastiya; Amung Logam Saputro
Jurnal Veteriner Vol 18 No 2 (2017)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (233.997 KB) | DOI: 10.19087/jveteriner.2017.18.2.175

Abstract

Zearalenone is a resorcylic acid lactone produced by fungal Fusarium graminearum in contaminated edible grains and can cause reproduction disorder in animals by binding to estrogen receptors on target cells. The aim of this study was to assess the potential use of aluminosilicates as mycotoxin binders to eliminate the adverse effect of zearalenone by examining the number of follicles and caspase-9 expression in the ovary of mice. The study adopted a completely randomized simple design using 20 mices which were randomly divided into five group each of which consisted of four mices. Five treatment groups consisted of K+ (without zearalenone and aluminosilicates); K- (treated with zearalenone 0.1 mg/mice/day); P1 (treated with zearalenone 0.1 mg/mice/day and aluminosilicates 0.5 mg/mice/day); P2 (treated with zearalenone 0.1 mg/mice/day and aluminosilicates 1 mg/mice/day); and P3 were treated with zearalenone 0,1 mg/mice/day and aluminosilicates 2 mg/mice/day) with gastric tube daily for 10 days. The data obtained from this study were analyzed by analysis of variance and proceeded with Duncan test. The result showed that the primary follicles, secondary follicles, tertiary follicles and de Graaf follicles increased significantly on P3 treatment group. Caspase-9 expressions decreased significantly in all of aluminosilicates groups as compared to positive control. The treatment of mice with zearalenone and aluminosilicates increases the number of follicles and decreased caspase-9 expression in the ovary of mice. ABSTRAK Zearalenon merupakan senyawa resorcylic acid lactone yang diproduksi oleh jamur Fusarium graminearum dan dapat mengakibatkan gangguan reproduksi pada ternak dengan membentuk ikatan pada reseptor estrogen. Penelitian ini bertujuan untuk menguji potensi aluminosilikat terhadap mencit yang telah dipapar zearalenon pada aspek jumlah folikel dan ekspresi caspase-9 organ ovarium. Penelitian ini menggunakan 20 ekor mencit yang dibagi menjadi lima kelompok perlakuan dengan masing-masing empat ulangan, yakni K+ tanpa dipapar zearalenon dan aluminosilikat; K- dosis zearalenon 0,1 mg/ekor/hari; P1 dosis zearalenon 0,1 mg/ekor/hari dan aluminosilikat 0,5 mg/ekor/hari; P2 dosis zearalenon 0,1 mg/ekor/hari dan aluminosilikat 1 mg/ekor/hari; P3 dosis zearalenon 0,1 mg/ekor/hari dan aluminosilikat 2 mg/ekor/hari dengan sonde lambung selama sepuluh hari. Data hasil skoring dan perhitungan dianalisis dengan sidik ragam dan dilanjutkan dengan uji Duncan. Hasil penelitian menunjukkan bahwa jumlah folikel primer, sekunder, tersier dan de Graaf pada P3 terjadi peningkatan yang signifikan. Ekspresi caspase-9 ovarium menunjukkan penurunan pada semua perlakuan aluminosilikat. Simpulan penelitian ini adalah mencit yang dipapar zearalenon dan kemudian diberi aluminosilikat mengalami peningkatan jumlah folikel dan penurunan ekspresi caspase-9 pada organ ovarium.
Penambahan Osteopontin dalam Pengencer Semen Beku Sapi Perah Friesian Holstein Meningkatkan Ekspresi B-Cell Cll/Lymphoma-2 Spermatozoa Postthawing (ADDITIONAL OSTEOPONTIN INTO FROZEN FRIESIAN-HOLSTEIN SEMEN DILUTER INCREASES THE EXPRESSION OF B-CELL CLL/L Abdul Samik; Yudit Oktanella; Tatik Hernawati; Ngakan Made Rai Widjaja; Intan Purwa Dewanti
Jurnal Veteriner Vol 15 No 4 (2014)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (145.606 KB)

Abstract

Post thawed frozen semen viability is one of the most important keys in artificial inseminationdepended on two major cell death mechanism, apoptosis and necrosis. It has been known that good fertilitydiary bull’s seminal plasma contains high concentration of osteopontin (OPN). Osteopontin also known ascell survival protein via inhibition to apoptotic cell death, and B-cell CLL/Lymphoma-2 (Bcl-2)expressionmostly related to the ability of cell survival. The aim of this study was to investigate the influence ofadditional OPN into frozen semen diluter on post thawed sperm Bcl-2 expression. Fresh semen collectedfrom ±4 year Friesian Holstein bull. Treatment group divided into four groups i.e.: control group (P0:without OPN supplementation), (P1: fresh semen with OPN supplementation 5 ?g/5.107 spermatozoa), (P2:fresh semen with OPN supplementation 10 ?g/5.107 spermatozoa), (P3: fresh semen with OPNsupplementation 20 ?g/5.107spermatozoa). Bcl-2 sperm expression was determined usingimunocytochemistry. The result of this study showed that there was no significant difference betweengroup P0 and P1 (p>0,05), but both group P2 and group P3 showed a significant difference with P0 (p<0,05).Nevertheless, there was no significant difference between group P2to group P3 on post thawed FriesianHolstein sperm Bcl-2 expression (p>0,05). The conclusion of this study is osteopontin supplement in frozensemen diluter is capable to increase post thawed Friesian Holstein sperm Bcl-2 expression.
Co-Authors Afiff , Usamah Anriansyah Renggaman Dea Indriani Astuti Dedi Rahmat Setiadi Hana Eliyani Imam Mustofa Intan Purwa Dewanti Kusnoto Kusnoto Lucia Tri Suwanti, Lucia Tri Mafatichul Firdaus Mufasirin MUHAMMAD AGIL Muhammad Thohawi Elziyad Purnama Ngakan Made Rai Widjaja Poedji Hastutiek Prastiya, Ragil Angga Raden Tatang Santanu Adikara Saputro, Amung Logam Soeharsono Soeharsono Surya Agus Prihatno Tatik Hernawati Tri Wahyu Suprayogi Warda Nafalizza Efendi Yeni Dhamayanti Yosua Kristian Adi Yudit Oktanella, Yudit