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ANTIBODY SCREENING EXAMINATION ON VOLUNTARY BLOOD DONORS AT BLOOD DONATION UNITS BANYUMAS REGENCY IN 2023 ETIVIA, DEVI; Riadi, Ahmad; widodo, widodo; Lestari, Sri
Journal of Applied Health Management and Technology Vol. 6 No. 1 (2024): April 2024
Publisher : Postgraduate Program , Poltekkes Kemenkes Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31983/jahmt.v6i1.11364

Abstract

Blood donation is a humanitarian activity where someone donates blood voluntarily. Blood Donation Units are required to provide safe and quality blood products to avoid transfusion reactions. One effort that can be made is to carry out pre-transfusion examinations on donor blood, one of which is an antibody screening examination. The aim of the antibody screening examination is to detect the presence or absence of clinically significant irregular antibodies in both the patient and donor. The aim of this study was to determine the blood type, gender and age of antibody screening in voluntary blood donors at the Blood Donation Unit. This type of research is descriptive using observational methods which was carried out in March 2023 at the Banyumas Regency Blood Donation Unit. Samples were taken using a total sampling technique, namely all voluntary blood donors who underwent an antibody screening test. Data collection was carried out using the qwalist diagast tool and laboratory archives which were accessed via the SIMDONDAR system. The results of the antibody screening research from a total of 3198 voluntary blood donors showed that the highest results were in blood type O, namely 1236 (39%) donors, in men, namely 2196 (69%), and in the elderly (26).-60 years).namely 1531 (48%) donors
Detection of rpoB Gene Mutations in Mycobacterium Tuberculosis widodo, widodo; Riadi, Ahmad; Etevia Purlinda, Devi; Setyowatiningsih, Lilik; roniafriansya, roni
Journal of Applied Health Management and Technology Vol. 5 No. 3 (2023): July 2023
Publisher : Postgraduate Program , Poltekkes Kemenkes Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31983/jahmt.v5i3.10093

Abstract

Perkembangan pengobatan infeksi tuberculosis memberikan harapan kesembuhan total akan tetapi seiring berjalanya waktu pengunaan obat berdampak pada munculnya strain Mycobacterium tuberculosis yang resisten terhadap obat peningkatan kecepatan diaknostik terhadap resistensi perlu dikembangkan salah satunya methode molekuler berbasis PCR penentu mutasi gen yang disebakan resistensi obat salah satu target obat yang digunakan adalah rpoB, pengunaan Primer rpoB sebagai penanda terjadinya mutasi diharapkan mampu memberikan dampak sebagai alternatif deteksi cepat secara molekuler. Panjang pita rpoB 318 bp cocok untuk kelompok Mycobacterium tuberculosis diperoleh hasil menunjukan pada kodon 509 perubagan AGC menjadi AAT terjadi perubahan asam amino dari serin menjadi Apargarin, perubahan nukleotida  CAG menjadi CCG pada kodon 510 mengubah asam amino Glutamin menjadi prolin, kodon 514 perubahan nukleotida TTC menjadi TTG mengubah asam amino Phenylalanine menjadi leucine, kodon 515 terjadi perubahan nukleotida ATG menjadi GTG perubahan ini menyebakan perubahan asam amino Methionine menjadi Valine  . perubahan tersebut menyebabkan terjadinya resistensi terhadap obat Rifampicin
MANAJEMEN BUDAYA LITERASI MELALUI KEGIATAN APEL PAGI DI MADRASAH ALIYAH NEGERI 3 MANDAILING NATAL Auliya Nasution, Mursyidan; Sulthan Arrasyid Nasution; Ananda Fahmul Afkar; Ahmad Riadi; Sunanto Nauli; Muharnita Azizah Rangkuti; Tauhid
Pendas : Jurnal Ilmiah Pendidikan Dasar Vol. 11 No. 01 (2026): Volume 11 No. 01 Maret 2026 Publish
Publisher : Program Studi Pendidikan Guru Sekolah Dasar FKIP Universitas Pasundan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23969/jp.v11i01.42978

Abstract

The low national literacy index necessitates innovative managerial re-engineering at the educational unit level to foster a competitive learning ecosystem. This study aims to analyze the management of literacy culture through the "Literacy Strengthening" program integrated into morning assembly activities at Madrasah Aliyah Negeri (MAN) 3 Mandailing Natal. Utilizing a descriptive qualitative approach, data were collected through one month of participatory observation, in-depth interviews with the headmaster, teachers, and students, as well as documentation studies via the madrasah's digital platform. The data were analyzed using an interactive model comprising data reduction, display, and verification. The results indicate that the headmaster performs a strategic role as an instructional leader through a systematic management cycle, including: (1) Participatory planning aligning literacy with the institutional vision; (2) Collaborative organizing with clear role distribution among teachers and students; (3) Consistent implementation through habituation methods, book reviews, and public speaking; and (4) Reflective supervision to maintain program quality. The transformation of the morning assembly from a mere administrative routine into a space for idea articulation is proven effective in enhancing students' self-confidence, public communication skills, and critical thinking. This study concludes that strengthening literacy culture based on institutional routine activities represents a best practice in internalizing "Kurikulum Merdeka" values and the "Profil Pelajar Pancasila," while serving as an acceleration model for human resource quality within the madrasah environment.
Perbandingan Berbagai Metode Pengecatan Spora pada Bacillus Cereus Widodo Widodo; Devi Etivia Purlinda; Ahmad Riadi
Jaringan Laboratorium Medis Vol 4 No 2 (2022): November 2022
Publisher : Poltekkes Kemenkes Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31983/jlm.v4i2.8493

Abstract

Endospores are formed by members of several genera of gram-positive bacteria, such as Bacillus and Clostridium. The spores are very resistant structurally; withstand the temperature of boiling water for two hours or more. They contain very little water and show very few chemical reactions. The favorable external environment will cause the spores to break down and the vegetative cells to appear to grow and reproduce. Identification of endospore-forming pathogens is important in food and medical microbiology. However, the spores contain multiple protective layers, which cannot be easily penetrated by simple staining or Gram staining techniques. Therefore, it is necessary to apply heat to help penetrate the color, with various methods of Gram stain, fluorescent spore painting, Schaeffer and Fulton spore painting and Client's spore painting method. The results of the fluorescent painting method have better results but require much more expensive equipment using a fluorescent microscope considering the facilities that must be provided for the Schaeffer and Fulton painting method as an alternative if they do not have these facilities.