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Extraction Of Brazilin From Sappan Wood as a Natural Reagent for Borax Detection by Digital Imaging Analysis Rismiarti, Zuri; Rahmat, Mamat; Nur Indra, Asep Iin; Syaindi, Amanda Afifah
Jurnal Kesehatan Prima Vol. 18 No. 2 (2024): AUGUST
Publisher : poltekkes kemenkes mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jkp.v18i2.1440

Abstract

The use of brazilien for borax detection is because the chemical properties of this compound are greatly influenced by pH which results in a change in the color of the brazilien. This compound gives an orange color at a pH range of 6-7 and yellow at an acidic pH, while at a pH range of 7-10 it gives a red color and at a pH >10 it gives a purplish red color. This research uses Brazilian extract from sappan wood for borax detection. By utilizing this compound extract, the use of chemical reagents for the detection of borax in food samples can be minimized. Apart from that, it can increase the added value of these crop commodities and can take advantage of the abundant natural resources in Indonesia. This research aims to develop a method that is accurate, simple and cheap and does not use special instruments to diagnose borax based on digital imaging, which includes extraction of brazilien from sappan wood using a maceration method using 96% ethanol solvent and water with a UV-Vis spectrophotometer, ratio optimization brazilin extract with boric acid, optimization of measurement time. The results of the research show that the solvent that provides optimum absorbance for brazilin extraction is water or distilled water, with a ratio of brazilin extract and boric acid of 1:1 and the measurement time is 5 minutes.
Development of an iron diagnostic method using Malaka fruit based on digital imaging Rismiarti, Zuri; Rahmat, Mamat; Indra, Asep Iin Nur; Hakimah, Nurul; Nurmayanti, Rani; Fadilah, Nur
Media Penelitian dan Pengembangan Kesehatan Vol. 36 No. 1 (2026): MEDIA PENELITIAN DAN PENGEMBANGAN KESEHATAN
Publisher : Poltekkes Kemenkes Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.34011/jmp2k.v36i1.4045

Abstract

Background: Iron (Fe) is a geochemically active element that needs to be monitored because excessive levels in water can reduce water quality and pose health risks. WHO sets a safe limit of Fe in drinking water at 0.3 mg/L. This condition demands an accurate, simple, and applicable Fe detection method without complex instruments. Objective: This study developed a digital imaging-based Fe(III) detection method using a natural reagent, malacca fruit extract, which is rich in gallic acid and forms a blue Fe-gallic acid complex. Methods: The study design included laboratory experiments involving gallic acid extraction, optimization of the Fe(III): extract ratio, optimization of measurement time, and evaluation of linearity, precision, accuracy, LoD, LoQ, and t-test against the UV-Vis spectrophotometry method. Results: The results showed an optimum Fe(III): extract ratio of 1:2, with reaction stability up to the 5th minute. A linear relationship was obtained in the range of 0.1–2 ppm (R² = 0.9969), with an LoD of 0.1377 ppm and a LoQ of 0.4589 ppm. The %RSD values ​​of 2.34–3.82% indicate good precision, while the accuracy ranges from 92.76–111.2%. A t-test confirmed that the digital imaging results were not significantly different from those of UV-Vis spectrophotometry. Conclusion: Overall, this method offers a portable, economical, and environmentally friendly analytical approach for Fe(III) detection and provides a basis for the development of digital application-based diagnostic systems using natural reagents.