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Bioremediation of the Lapindo mud contaminated soil using eco-enzyme: Case of a total petroleum hydrocarbon removal Sulistiyaningrum, Dwi Rakhmatun; Rumhayati, Barlah; Srihardyastutie, Arie
Journal of Degraded and Mining Lands Management Vol. 13 No. 1 (2026)
Publisher : Brawijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15243/jdmlm.2026.131.9659

Abstract

This research investigated the effectiveness of bioremediation of Lapindo mud-contaminated soil using an eco-enzyme for total petroleum hydrocarbons (TPH) removal. The soil samples were collected from Glagaharum and Gedang Villages, Sidoarjo, East Java, Indonesia, which have been contaminated by the Lapindo mud containing TPH. The bioremediation experiment was carried out ex situ in a container filled with contaminated soil samples, which were watered with eco-enzymes daily. Bioremediation time was optimized at 0, 7, 4, 21, and 28 days while the eco-enzymes concentration was varied at 0, 5, 10, and 15% (v/v). The concentration of TPH in the soil samples was analyzed using the gravimetric method at 0, 7, 4, 21, and 28 days. The results showed that TPH concentration was gradually decreased during remediation. The remediation removal percentage reached an optimum after 21 days. The TPH was optimally removed using the eco-enzyme at 15% (v/v), achieving 95.39%. The bioremediation of contaminated soil using eco-enzymes also increased the contents of total C and total N by 50.97% and 39.43%, respectively. However, this treatment reduces total P by 50.89%. Furthermore, the pH and temperature values for the three samples before and after the bioremediation process remained fairly stable, within the pH range of 6-7 and the temperature range of 25.7-26.2 °C.
THE DEVELOPMENT OF GLUCOSE SENSOR BASED ON SiO2-CuO MATERIALS USING SCREEN PRINTED CARBON ELECTRODE (SPCE) Utamiyanti, Intan Frina; Rumhayati, Barlah; Mulyasuryani, Ani
ALCHEMY Jurnal Penelitian Kimia Vol 12, No 1 (2016): March
Publisher : UNIVERSITAS SEBELAS MARET (UNS)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/alchemy.12.1.942.50-60

Abstract

The development of material-based glucose sensor SiO2-CuO using Screen Printed Carbon Electrode (SPCE) had been done. Three types of materials were used to detect glucose, i.e Multi Wall Carbon Nanotube (MWCNT)-SiO2-CuO, SiO2-CuO (A) dan SiO2-CuO (B). The differences composition of SiO2-CuO(A) and SiO2-CuO(B) occurred during the addition of NaOH in synthesis process of SiO2-CuO (B). The prepared materials were analyzed by Scanning Electron Microscopy (SEM), cyclic voltammetry method and chrono-amperometry. Cyclic voltammetry analysis was conducted at a potential range of -1.0 - 1.5 V with Ag/AgCl as reference electrode. The scan rate was 100 mV/sec and the potential was varied at (-0.6), (-0.5) and (-0.4) V, in which the duration of each analysis was 5 second. Based on the result of analysis, whether by SEM, cyclic voltammetry and chrono-amperometry, the SiO2-CuO (B) was found to be the best material for detection of glucose.
The Effect of Coagulant Particle Size, Coagulant and Flocculant Dose on Remazol Yellow Dye Removal Effectiveness Wijaya, Khalida; Fardiyah, Qonitah; Rumhayati, Barlah
JKPK (Jurnal Kimia dan Pendidikan Kimia) Vol 10, No 3 (2025): JKPK (Jurnal Kimia dan Pendidikan Kimia)
Publisher : Program Studi Pendidikan Kimia FKIP Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/jkpk.v10i3.109798

Abstract

Textile dye effluents are hazardous because many residual dyes are persistent, toxic, and potentially carcinogenic. This study assessed an environmentally friendly coagulation flocculation route to remove Remazol Yellow, an azo dye used in the batik industry in South Sulawesi, by substituting synthetic reagents with papaya (Carica papaya) seeds as a coagulant and Aloe vera gel as a flocculant. FTIR confirmed hydroxyl, carboxyl, and amine groups in papaya seeds, while Aloe vera gel was dominated by polysaccharide and carboxylate features. Jar tests were performed at room temperature using an initial dye concentration of 30 mg L−1. Coagulation was conducted for 5 min at 950 rpm, followed by flocculation for 30 min at 125 rpm and sedimentation for 30 min. Residual dye was measured by UV Vis spectrophotometry at 417 nm. Operational variables were optimized, including coagulant particle size (30 to 100 mesh), papaya seed dose (10 to 50 mg L−1), and Aloe vera gel dose (10 to 60 g L−1). The optimum particle size was 70 mesh, giving 60.4 percent removal. The optimum papaya seed dose was 20 mg L−1 with 83.5 percent removal, and the optimum Aloe vera gel dose was 50 g L−1, achieving 97.4 percent removal. These results demonstrate the potential of papaya seeds and Aloe vera gel as effective and sustainable materials for batik dye wastewater treatment.