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All Journal Jurnal Sains dan Teknologi Farmasi Jurnal Penelitian Pendidikan IPA (JPPIPA) JSFK (Jurnal Sains Farmasi & Klinis) JFIOnline Warta Pengabdian Andalas: Jurnal Ilmiah Pengembangan Dan Penerapan Ipteks Jurnal Altifani Penelitian dan Pengabdian kepada Masyarakat Jurnal Ilmu Kesehatan Indonesia (JIKSI) Jurnal Pengabdian Masyarakat Dharma Andalas Abdimas Mandalika

Rustini Rustini

Andalas University, Padang

Author-ID : 366374

Religion Aerospace Engineering Agriculture, Biological Sciences & Forestry Arts Humanities Astronomy Automotive Engineering Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Dentistry Earth & Planetary Sciences Economics, Econometrics & Finance Education Electrical & Electronics Engineering Energy Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Medicine & Pharmacology Nursing Physics Public Health Social Sciences Other

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Uji Aktivitas Antibakteri Ekstrak Bakteri Endofit dan Identifikasi Bakteri yang Diisolasi dari Kulit Jeruk Nipis (Citrus aurantifolia Swingle) Rustini Rustini; Friardi Ismed; Gina Sasha Nabila
Jurnal Sains Farmasi & Klinis Vol 9, No 1 (2022): J Sains Farm Klin 9(1), April 2022
Publisher : Fakultas Farmasi Universitas Andalas

Kulit Jeruk nipis (Citrus aurantifolia Swingle) merupakan bagian tanaman yang banyak digunakan untuk pengobatan dengan berbagai aktivitas farmakologis. Namun masih sedikit diketahui kajian tentang bakteri endofit yang terkait dengan kulit buah tanaman ini. Penelitian ini bertujuan untuk mengisolasi bakteri endofit dari kulit buah tanaman ini serta menguji aktivitas dalam mengambat pertumbuhan bakteri Staphylococcus aureus, Escherichia coli dan Methicilin-Resistant Staphylococcus aureus (MRSA). Sebanyak enam Isolat bakteri berhasil diisolasi lalu difermentasi dalam media Nutrient Broth (NB). Hasil fermentasi kemudian diekstraksi menggunakan pelarut etil asetat. Ekstrak kental masing-masing isolat diujikan aktivitas antibakterinya menggunakan metode difusi Kirby-Bauer. Berdasarkan hasil pengukuran zona hambat menunjukkan bahwa ekstrak dari isolat 3 dan isolat 5 memiliki diameter hambat kuat >10 mm. Hasil identifikasi fitokimia secara kualitatif menunjukkan bahwa ekstrak dari isolat 3 menujukkan adanya kandungan alkaloid dan flavonoid sedangkan isolat 5 teridentifikasi senyawa alkaloid, flavonoid dan polifenol. Hasil identifikasi penamaan bakteri endofit menunjukkan bahwa isolat 3 merupakan Bacillus velezensis strain JS25R dan isolat 5 merupakan Staphylococcus sp. Ekstrak dari bakteri endofit ini terbukti memiliki aktivitas terhadap bakteri patogen dan potensial untuk dilakukan isolasi dan pengembangan terhadap senyawa metabolit sekunder yang memiliki aktivitas tersebut.

Penyuluhan Pemanfaatan Minyak Atsiri Cengkeh dan Serai sebagai Aromaterapi Aplikasi Parfum Masker Lusia Eka Putri; Rustini Rustini; Sefrianita Kamal; Sara Surya; Rosiana Rizal; Nofrizal Nofrizal; Nurunnisa Aprilya Yulfi; Widia Rahma Putri; Laras Alhabil
Jurnal Pengabdian Masyarakat Dharma Andalas Vol 1 No 1 (2022): Jurnal Pengabdian Masyarakat Dharma Andalas
Publisher : LPPM Universitas Dharma Andalas

Indonesia is one of the countries that has the potential as a producer of essential oils. Plant parts that can be used as a source of essential oils are the roots, stems, leaves, flowers, and so on. In the midst of the current pandemic, where the number of COVID-19 cases is increasing day by day. This requires us to maintain our immune system and always comply with health protocols, one of which is the use of masks. The use of the mask for more than 4-5 hours will cause an unpleasant odor. The outreach activity on the use of essential oils of citronella and cloves as aromatherapy in the application of perfume masks is an alternative that can be used by the public to overcome the negative impacts of using masks that last more than 4-5 hours. With this outreach activity, it is hoped that the community can apply, implement and stay healthy in the midst of the epidemic that hit. In maintaining endurance, we must relax ourselves so that we can foster creativity and remain productive in carrying out daily activities.

Edukasi Peduli Obat “Dagusibu” (Dapatkan, Gunakan, Simpan, Buang) M.Rifqi Efendi; Mesa Sukmadani Rusdi; Rustini Rustini; Sefrianita Kamal; Sara Surya; Lusia Eka Putri; Afriyani Afriyani
Abdimas Mandalika Vol 1, No 1 (2021): Agustus
Publisher : Universitas Muhammadiyah Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31764/am.v1i1.5790

DAGUSIBU (Dapatkan, Gunakan, Simpan, Buang) merupakan bagian dari Gerakan Keluarga Sadar Obat (GKSO) yang diprakarsai Ikatan Apoteker Indonesia (IAI) dalam mencapai pemahaman dan kesadaran masyarakat, khususnya pada ibu – ibu terhadap penggunaan obat dengan benar. Kegiatan penyuluhan Peduli Edukasi ObatDAGUSIBU memiliki tujuan membantu masyarakat setempat,khususnya ibu - ibu dalam pengelolaan obat baik di lingkungan keluarga maupun masyarakat secara umum. Penyuluhan dilakukan dengan metode pemberdayaan masyarakat partisipatif, yaitu metode yang menekankan keterlibatan masyarakat pada rangkaian kegiatan. Kegiatan penyuluhan berjalan dengan lancar dan sukses. Berdasarkan obersevasi, terdapat peningkatan pemahaman dan pengetahuan ibu ibu PKK menjawab dengan benar pertanyaan dari tim penyuluh.

Edukasi Titik Kritis Kehalalan Produk Pangan, Kosmetik, dan Obat-Obatan Menggunakan Flowchart pada Siswa Sekolah Menengah Analis Kimia (SMAKPA) Padang Dini Hanifa; Rustini Rustini; Purnawan Pontana Putra
Warta Pengabdian Andalas Vol 30 No 4 (2023)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat (LPPM) Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jwa.30.4.798-806.2023

Halal products are crucial for consumers. In Islam, halal and wholesome food consumption is vital for maintaining physical and spiritual well-being. The Indonesian government mandates halal product certification by Law No. 33 of 2014 on Halal Product Assurance. The critical points in determining the halal status of a product refer to processes or ingredients in its production that may potentially involve, contain, or mix with haram substances. Nevertheless, many students lack a clear understanding of halal and haram principles. This knowledge is essential to help students avoid using forbidden ingredients in Islamic teachings. Padang Chemical Analyst High School (SMAKPA) students are the targets of this service because vocational school students must have work-ready skills. Before the education session, a pre-test was conducted to assess the student's understanding of the material presentation. Education was delivered using a flowchart to enhance engagement and comprehension. The content encompasses the concepts of halal and haram in Islam, the responsible parties for halal certification, halal logos, and the analysis of critical points in the halal status of food, cosmetics, and medications. Following the material presentation, a post-test was administered, followed by a question and answer session to evaluate the students' understanding. Pre-tests and post-tests were employed to assess student comprehension, with pre-test scores averaging 99 and post-test scores at 99.5. Through this educational initiative, students gain a comprehensive understanding of the Critical Points of Halal Status in Food, Cosmetics, and Medications using a flowchart

Aktivitas Penghambatan Enzim α-Glukosidase dari Ekstrak Hasil Fermentasi Bakteri Endofit dari Tapak Dara (Catharanthus roseus (L.) G. Don) Daun Ulfa Rosiatul Huda; Friardi Ismed; Nurwahidatul Arifah; Valdy Filando Sardy; Rustini
Jurnal Penelitian Pendidikan IPA Vol 10 No 10 (2024): October
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v10i10.9178

Catharanthus roseus (L.) G. Don contain bioactive compounds that act as inhibitors against the α-glucosidase enzyme. This study aims to test the α-glucosidase enzyme inhibitor activity of fermented extract of endophytic bacteria from tapak dara leaves and identify the bacterial species. Endophytic bacteria were isolated using a direct planting method, resulting in eight isolates. The bacteria were fermented for 24-72 hours in nutrient broth media. Ethyl acetate was used to extract the fermented bacteria. Screening for α-glucosidase enzyme inhibitory activity was conducted using the TLC bioautography method. Four bacterial isolates (TD 1, TD 3, TD 4, TD 7) produced clear zones, qualitatively indicating that the isolates have inhibitory activity against the α-glucosidase enzyme. The IC50 results from the eight isolates showed that three isolates provided strong IC50 values: isolate TD 1 at 19.93 µg/ml, isolate TD 2 at 92.34 µg/ml, and isolate TD 4 at 13.94 µg/ml, compared to acarbose at 48.85 µg/ml). Molecular identification showed that isolate TD 1 was Pseudomonas oryzihabitans and isolate TD 4 was Staphylococcus warneri. This study concluded that endophytic bacteria from tapak dara leaves have the potential as a source of α-glucosidase enzyme inhibitor compounds for the development of antidiabetic drugs.

Isolasi dan Pengujian Aktivitas Penghambat Enzim Alfa Glukosidase dari Bakteri Endofit Daun Kumis Kucing (Orthosiphon aristatus (Blume) Miq.) Indah Tamara Herman Putri; Rustini; Friardi Ismed; Valdy F Sardi; Nurwahidatul Arifah
Jurnal Penelitian Pendidikan IPA Vol 10 No 10 (2024): October
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v10i10.9264

Orthosiphon aristatus (Blume) Miq., commonly known as kumis kucing, is frequently used in traditional Indonesian medicine to treat diabetes, hypertension, rheumatism, gout, and as a diuretic. However, large-scale production of medicinal products from natural sources requires significant raw material and land, creating a need for alternative methods. This study explores the potential of isolating endophytic bacteria from kumis kucing leaves to serve as α-glucosidase enzyme inhibitors, offering a more sustainable approach. Eight bacterial endophytes were isolated and tested using bioautography assays and IC50 measurements to assess their inhibitory activity. Four isolates—KK 1, KK 3, KK 4, and KK 5—demonstrated significant α-glucosidase inhibition, with IC50 values of 41.35 µg/mL, 57.04 µg/mL, 56.70 µg/mL, and 164.16 µg/mL, respectively. Molecular testing identified KK 1 as Priestia aryabhattai, while KK 3 and KK 5 were identified as Priestia megaterium. These findings suggest that endophytic bacteria isolated from Orthosiphon aristatus leaves have the potential to be developed as natural sources of α-glucosidase inhibitors, which are beneficial for diabetes management.

Co-Authors Afriyani Afriyani Afriyani Akmal Djamaan Andani Eka Putra Arfiandi Arfiandi Asiska Permata Dewi Dachriyanus Densi Selpia Sopianti Densi Selpia Sopianti Dian Handayani Dini Hanifa Diva Diana Erizal Zaini Fino Nauvalino Fitria Rahmi Friardi Ismed Gina Sasha Nabila Hari, Dewi Gulyla Henny Lucida Honesty, Resta Honesty, Resta Indah Tamara Herman Putri Laras Alhabil Lusia Eka P Putri Lusia Eka Putri Lusia Eka Putri M. Rifqi Efendi Marlina . Melzi Octaviani Mesa Sukmadani Rusdi Muhammad Hidayat Nadhifah Salsabila Nofrizal Nurunnisa Aprilya Yulfi Nurwahidatul Arifah Purnawan Pontana Putra Rosiana Rizal Sara Surya Sara Surya Sara Surya Saufitri, Dian Saufitri, Dian Sefrianita Kamal Sefrianita Kamal Sefrianita Kamal Telsa Dwiana Hapsari Ulfa Rosiatul Huda Valdy F Sardi Valdy Filando Sardy Vitresia, Havriza Widia Rahma Putri Yelly Oktavia Sari Yohannes Alen Yufri Aldi

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