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Edhi Sandra
Departemen Konservasi Sumberdaya Hutan Dan Ekowisata, Fakultas Kehutanan Dan Lingkungan, Universitas IPB
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Control & Systems Engineering Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Energy Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Materials Science & Nanotechnology Social Sciences Other

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Optimasi Sterilisasi Eksplan Daun Tanaman Lidah Mertua (Sansevieria sp.) pada Kultur In Vitro Ade Tiyan Handayani; Edhi Sandra; Hanik Faizah
Bioscientist : Jurnal Ilmiah Biologi Vol 10, No 1 (2022): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v10i1.4808

Abstract

The existence of Sansevieria sp. as an ornamental plant that has many benefits preserved. However, conventional propagation requires a lot of materials and time long. Leaf cuttings are only able to produce 1-2 plants in 2 months, and stop when they are aged 5 months. While the separation of tillers (Tillering) only produces 2-3 plants from 1 clump for 5 months. Tissue culture can be the solution, but sterilization must be done to minimize contamination without killing explants. The research aims to find out optimal concentration of HgCl2 and duration of shaking on the sterilization of Sansevieria leaf explants sp.. The research design used a completely randomized design with 2 factors. first factor namely the concentration of HgCl2 with 3 levels (4%, 7%, and 10%,) and the second factor is duration shuffled with 4 levels (3, 5, 7, and 9 minutes). Each treatment includes a negative control performed at the time of explant sterilization in Laminar Air Flow. Data analyzed by test ANOVA or Kruskal-Wallis. Followed by the 5% BNJ test if it is significantly different. Results show that the concentration of HgCl2 has a significant effect on the parameters of the initial time of contamination, the mass sterility of explants, and percentage of browning explants, but had no significant effect on percentage of contaminated explants. Concentration that is too high can slow down the appearance of contamination, prolonging the sterile period, but causing browning of explants. Known that the duration of explant shaking in HgCl2 had no significant effect on all parameter. The use of 10% HgCl2 with a shaking duration of 7 minutes (H3P3) is known to be the most effective optimal for sterilization of explants Sansevieria sp. because it is able to reduce external contamination, prolongs the sterile period up to 12.33 HST, and does not cause too many explants browning that is 33.33%.
PENGARUH JENIS EKSPLAN DAN KOMPOSISI MEDIA TERHADAP PEMBENTUKAN EMBRIO SOMATIK TANAMAN AGLAONEMA ACEH (AGLAONEMA ROTUNDUM) SECARA IN VITRO Hilda Wijaya; Ani Lestari; Edhi Sandra
Jurnal AGROHITA: Jurnal Agroteknologi Fakultas Pertanian Universitas Muhammadiyah Tapanuli Selatan Vol 7, No 4 (2022): JURNAL AGROHITA
Publisher : Fakultas Pertanian Universitas Muhammadiyah Tapanuli Selatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31604/jap.v7i4.7365

Abstract

Penelitian tentang embriogenesis somatik aglaonema aceh (Aglaonema rotundum) telah dilaksanakan pada bulan November 2021 sampai Maret 2022 di Laboratorium Kultur Jaringan Esha Flora, Bogor, Jawa Barat. Penelitian ini bertujuan untuk mendapatkan formulasi terbaik antara jenis eksplan dan komposisi media terhadap pertumbuhan embrio somatik tanaman aglaonema aceh (Aglaonema rotundum). Metode yang digunakan adalah Rancangan Acak Lengkap (RAL) Faktorial yang terdiri dari petak utama yaitu jenis eksplan dan anak petak yaitu komposisi media. Masing-masing faktor terdiri dari 3 dan 4 taraf perlakuan, sehingga dihasilkan 12 kombinasi perlakuan yang diulang sebanyak 3 kali. Data dianalisis menggunakan uji ANOVA taraf 5% dan diuji lanjut menggunakan DMRT (Duncan Multiple Range Test) taraf 5%. Eksplan yang digunakan berasal dari planlet tanaman aglaonema aceh (Aglaonema rotundum) yang berumur 1 tahun setelah kultur. Hasil penelitian menunjukkan bahwa jenis ekplan  tunas dan komposisi media M2 (MS + TDZ 2mg/l + 2,4D 4mg/l + picloram 2mg/l + GA3 2mg/l + 2-iP 8mg/l) masing-masing menunjukkan hasil terbaik pada persentase eksplan membentuk embrio somatik langsung dan waktu muncul embrio. Sedangkan kombinasi jenis eksplan tunas dengan komposisi media M3 (MS + TDZ 2mg/l + 2,4D 4mg/l + picloram 2mg/l + GA3 2mg/l + Kinetin 8mg/l) memberikan hasil terbaik pada persentase eksplan membentuk kalus embriogenik dan skor induksi embriogenesis somatik.
Pengaruh Beberapa Formulasi Sitokinin Terhadap Penyediaan Bibit dan Pertumbuhan Eksplan Tanaman Pule Pandak (Rauvolfia serpentina (L.) Benth. ex Kurz) Secara In Vitro Anita Widya Karyaningtyas; Ani Lestari; Edhi Sandra
JURNAL AGROPLASMA Vol 10, No 1 (2023): JURNAL AGROPLASMA VOLUME 10 NO 1 TAHUN 2023
Publisher : UNIVERSITAS LABUHANBATU

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/agroplasma.v10i1.3914

Abstract

Propagation of pule pandak (Rauvolfia serpentina (L.) Benth. Ex Kurz) conventionally shows growth from seeds and stem cuttings of less than 15%. The low growth percentage is due to the seeds have hard coats, so the germination of the seeds is very low. One of the technologies that can be used and provides hope in the supply of seeds in large quantities and in a relatively short time is the in vitro culture technique. The aim of this study was to determine does the several cytokinin formulations administration affected the growth of pule pandak (Rauvolfia serpentina (L.) Benth. ex Kurz) plant explants. The study was conducted at Esha Flora, Bogor from April 2022 to July 2022. The experiment used a single factor Completely Randomized Design (CRD) consisting of 1 factor using 4 combinations of cytokinins (2-IP, TDZ, BAP and Kinetin) and repeated 10 times so that there are 40 experimental units for 12 weeks. The data obtained were then analyzed using the ANOVA test at a significant level of 5%. The results showed that the S1 treatment (Modified MS Media + 2-IP 2 mg/l + TDZ 0.1 mg/l + BAP 0.5 mg/l and Kinetin 0.5 mg/l) was the best cytokinin formulation to produce the highest average number of shoots was 8.87 shoots, the highest explant height was 2.49 cm and the fastest average of somatic embryogenesis emergence time was 23.3 DAP. Somatic embryo phase in treatment S1, S2 and S3 experienced globular, heart and torpedo phases. Keywords: Cytokinine, Somatic Embryogenesis, Tissue Culture, Rauvolfia serpentina (L.) Benth. Ex Kurz
Konservasi In Vitro Jenis Tumbuhan Gambut Tumih (Combretocarpus rotundatus (Miq.) Danser) Istomo Istomo; Edhi Sandra; Vianti Vianti; Mufti Abdillah
HUTAN TROPIKA Vol 18 No 1 (2023): Volume 18 Nomor 1 Tahun 2023
Publisher : Jurusan Kehutanan, Fakultas Pertanian Universitas Palangka Raya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36873/jht.v18i1.7867

Abstract

Tumih (Combretocarpus rotundatus (Miq.) Danser) is one of the local species recommended for disturbed peatland rehabilitation activities. This species can be classified as a fast-growing and tolerant type to dry and open conditions. The aim of this study is to identify the success of explant preparation techniques and tumih explant sterilization seen from the survival rate, contamination level, and browning level. The research was conducted at the Environmental Biotechnology Laboratory, Environmental Research Center (PPLH), IPB. The plant material used was tumih shoots that were sterilized using detergent, HgCl2, Clorox, and rinsed with sterile water. The explants were initiated on MS media with the addition of BAP, namely 0 ml/l; 0.5 ml/l; 1 ml/l; and 1.5 ml/l, and TDZ, namely 0 ml/l; 0.05 ml/l; 0.1 ml/l; and 0.5 ml/l. This study used 16 treatments with 7 replications. The results showed that the average survival rate percentage of tumih explants reached 22.32%, fungal contamination was 57.14%, bacterial contamination was 1.79%, and the average browning percentage in the explants was 18.75%. The preliminary study that has been carried out is categorized as successful, with 25 explants still maintaining green leaves and stems
Isolasi dan Identifikasi Cendawan Endofit Akar Anggrek Epifit dan Hemiepifit: Isolation and Identification of Endophytic Fungi in Epiphytic and Hemiepiphytic Orchid Root Michael Michael; Nampiah Sukarno; Sofi Mursidawati; Edhi Sandra; Nadiya Dwi Rahayu
Jurnal Sumberdaya Hayati Vol. 9 No. 4 (2023): 2023
Publisher : Departemen Biologi, Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jsdh.9.4.152-163

Abstract

Orchids form a symbiotic relationship with endophytic fungi throughout their life cycle, including their seed germination. Research on orchid endophytic fungi in Indonesia is still limited. This study aimed to isolate and identify endophytic fungi of Phalaenopsis amabilis, Dipodium brevilabium, Vanilla planifolia, and Aerides odorata orchids. Endophytic fungi were isolated from surface-sterilized orchid root pelotons. Fungal identification was based on morphological and molecular characteristics using ITS rDNA sequences, followed by BLAST and phylogenetic tree analysis. All orchids studied had peloton structures in root cortex tissues. From the four orchids studied, 22 fungal isolates were obtained. Five fungal isolates were obtained from the P. amabilis orchid, consisting of 1 Penicillium and 4 sterile mycelium isolates. The D. brevilabium had 7 fungal isolates, namely 1 Penicillium and 6 Fusarium isolates. The V. planifolia had 6 Fusarium isolates, whereas the A. odorata had 4 isolates, such as 1 Penicillium, 2 sterile mycelium, and 1 unidentified isolate. The genus Penicillium was found in 3 out of the 4 orchid species studied. One Penicillium isolate was molecularly identified as Penicillium citrinum. Endophytic fungi in P. amabilis were mostly sterile mycelium, while D. brevilabium, V. planifolia, and A. odorata were dominated by Fusarium.
Pengaruh Konsentrasi 2ip Terhadap Pembentukan Tunas dari Embrio Somatik Tanaman Aglaonema dud anjamani Secara In Vitro Yana, Kholika; Win Ariga Mansur Malonga; Edhi Sandra
BIOMARAS : Journal of Life Science and Technology Vol 2 No 1 (2024): BIOMARAS : Vol 2, No 1 Februari 2024
Publisher : Fakultas Ilmu dan Teknologi Hayati Universitas Teknologi Sumbawa

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Aglaonema di Indonesia lebih dikenal dengan Sri Rejeki. Salah satu jenis Aglaonema yang populer dikenal dimasyarakat ialah Aglaonema “dud anjamani”. Aglaonema jenis ini merupakan Aglaonema hibrida yang berasal dari Thailand yang memiliki perpaduan warna hijau terang dan merah terang, bentuk daunnya membulat sehingga lebih memudahkan susunan daunnya membentuk roset. Sebagai penerapan bioteknologi modern, kultur in vitro telah terbukti dapat digunakan untuk menghasilkan benih tanaman secara masal dan cepat. Penggunaan teknik kultur in vitro juga tidak membutuhkan ruangan yang luas dibandingkan budidaya tanaman secara konvensional. Serta tanaman yang dihasilkan bebas dari penyakit, hama, dan virus karena kondisi lingkungan kultur yang aseptik. Pada penelitian ini menggunakan embrio somatik tanaman Aglaonema dud anjamani dengan 5 perlakuan yaitu kontrol MS0, MS0 + 2ip dengan konsentrasi 2ip (0.5mg/L, 1mg/L, 1,5mg/L dan 2mg/L). Penelitian ini menggunakan teknik subkultur. Hasil pertumbuhan tunas dengan penambahan hormon 2ip pada setiap perlakuan dengan konsentrasi yang berbeda-beda memberikan respon pada pertumbuhan tunas dan jumlah tunas yang berhasil terbentuk dari masing-masing perlakuan, pada minggu ke-12 berdasarkan menunjukkan bahwa perlakuan kontrol dan perlakuan 2ip dengan konsentrasi 2ml berbeda nyata dengan perlakuan 2ip dengan konsentrasi 1ml dan perlakuan 2ip dengan konsentrasi 1.5mg/L, serta berbeda sangat nyata dengan perlakuan 2ip dengan konsentrasi 0.5mg/L.
Subkultur Dendrobium kanayao secara In Vitro di Esha Flora Hardiyanti Pebrian, Rini; Lili Suharli; Edhi Sandra
BIOMARAS : Journal of Life Science and Technology Vol 2 No 1 (2024): BIOMARAS : Vol 2, No 1 Februari 2024
Publisher : Fakultas Ilmu dan Teknologi Hayati Universitas Teknologi Sumbawa

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Abstract

Anggrek memiliki nilai keindahan pada bunganya dan sangat populer di kalangan masyarakat. Anggrek memiliki bentuk unik dan warnanya menarik sehingga di sebut “Queen of flower”. Dendrobium kanayao merupakan salah satu jenis anggrek yang paling banyak diminati karena mudah dirawat, serta warna dan bentuk bunga beragam. Namun terdapat kendala dalam mengembangkan budidaya anggrek secara konvensional sehingga perlu dilakukan teknik kultur jaringan untuk perbanyakan tanaman dengan cepat dan seragam. Dalam kultur jaringan permasalahan yang dihadapi yaitu adanya kontaminasi pada tahap subkultur maka penelitian ini bertujuan untuk mendapatkan kultur steril tanaman Dendrobium kanayao dengan cara In Vitro di Esha Flora. Metode yang dilakukan yaitu subkultur eksplan Dendrobium kanayao pada media growmore dan dilakukan pengamatan meliputi waktu pertama terjadinya kontaminasi, persentase kultur steril, kultur terkontaminasi jamur, dan bakteri. Hasil penelitian menunjukkan persentase subkultur Dendrobium kanayao mendapatkan 100% kultur steril namun beberapa eksplan mengalami browning. Disimpulkan bahwa subkultur Dendrobium kanayao menggunakan media growmore dapat dikatakan berhasil meskipun beberapa eksplan mengalami browning.
Isolasi dan Identifikasi Cendawan Endofit Akar Anggrek Epifit Vanda sp. dan Anggrek Terestrial Spathoglottis plicata: Isolation and Identification of Root Endophytic Fungi in Epiphytic orchid Vanda sp. and Terrestrial Orchid Spathoglottis plicata Rahayu, Nadiya Dwi; Listiyowati, Sri; Rafi, Mohamad; Sandra, Edhi; Mursidawati, Sofi; Risna; Sukarno, Nampiah
Jurnal Sumberdaya Hayati Vol. 10 No. 4 (2024): 2024
Publisher : Departemen Biologi, Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jsdh.10.4.198-204

Abstract

Endophytic fungi play an important role in the orchid lifecycle by aiding seed germination and plant development. Information on the diversity of endophytic fungi in Indonesian orchids, particularly Vanda epiphytic orchid and Spathoglottis terrestrial orchid, is still limited. This study aimed to isolate and identify endophytic fungi from the roots of Vanda sp. and Spathoglottis plicata orchids collected from Banjarbaru, South Kalimantan, and Kendari, Southeast Sulawesi. Isolation was performed using Potato Sucrose Agar (PSA) medium, and morphological identification was carried out through macroscopic and microscopic observations. A total of four endophytic fungal isolates were identified, including Nigrospora sp. and Penicillium sp. from Vanda sp. roots, and two isolates of Fusarium sp. from Spathoglottis plicata roots. The four fungal isolates obtained had aerial mycelium and septate hyphae, as well as conidia with diverse shapes. The diversity of endophytic fungi in orchid species could be influenced by the fungal affinity on the specific microhabitat conditions of each tissue and host plant species.
Keberhasilan Inisiasi Eksplan KEBERHASILAN INISIASI EKSPLAN TUNAS DAN DAUN GMELINA (Gmelina arborea L.) DENGAN PENERAPAN BERBAGAI METODE STERILISASI Wulandari, Arum Sekar; Sandra, Edhi; Dian Kirani, Annisa
Journal of Tropical Silviculture Vol. 16 No. 02 (2025): Jurnal Silvikultur Tropika
Publisher : Departemen Silvikultur, Fakultas Kehutanan dan Lingkungan, Institut Pertanian Bogor (IPB)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/j-siltrop.16.02.107-115

Abstract

Gmelina is a fast growing species that has high sufficient economy value and useful as medicinal plant. Propagation of gmelina by tissue culture has not been widely applied in Indonesia. This study aims to analize the effect of explant types and sterilization methods on the success of gmelina initiation. Treatments used in this study are explant types (apical shoot and leaf) and four sterilization methods (B1-B4) using detergent, tween 80, fungicide, bactericide, NaOCl, and HgCl2. All sterilization methods can produce 6‒19% sterile gmelina shoot culture, but has not succeded producing sterile gmelina leaf culture. Contamination by fungi and bacteria is the main cause of failed gmelina shoot and leaf culture initiation. In general, sterilization methods used in this study has succeeded in eliminating microbes on the explant’s surface, but has not succeeded in eliminating microbes inside the explant.
Co-Authors . Siswoyo Ade Tiyan Handayani Agus Hikmat Anggit Haryoso Ani Lestari Anita Widya Karyaningtyas Arum Sekar Wulandari Daru Mulyono Daru Mulyono Diah Ratnadewi Dian Kirani, Annisa Ervizal A. M. Zuhud Ervizal A.M. Zuhud Fauzan, Yusuf Sigit Ahmad Fauzan, Yusuf Sigit Ahmad Hanik Faizah Hardiyanti Pebrian, Rini Heru Sugito Hilda Wijaya Istomo . Lilik Listiani M. Rafi Michael Michael Mufti Abdillah Muhammad Syafii Nadiya Dwi Rahayu Nampiah Sukarno Nurcahyo Widyodaru Nurcahyo Widyodaru Nurul Kamila Primadika Al Manar Rahayu, Nadiya Dwi Risna Rita Kartika Sari RUSMILAH SUSENO Setya Kurniawan Sofi Mursidawati Sofi Mursidawati Sri Listiyowati Suharli, Lili Sulistyo Sidik Purnomo Syafitri Hidayati Vianti Vianti Win Ariga Mansur Malonga Windi Widia Nengsih Yana, Kholika YANTO SANTOSA Yusuf Sigit Ahmad Fauzan