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Pelatihan Penerapan Sinkronisasi Estrus dan Inseminasi Buatan untuk Peningkatan Produktivitas Budidaya Domba di Desa Pamulihan Kecamatan Pamulihan Kabupaten Sumedang Solihati, Nurcholidah; Rasad, Siti Darodjah; Setiawan, Rangga; Widyastuti, Rini; Sari, Aprilianna P.Z.N.L.; Toha, Toha; Winangun, Kikin
Farmers : Journal of Community Services Vol 7, No 1 (2026): Farmers: Journal of Community Services
Publisher : Unpad Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/fjcs.v7i1.69538

Abstract

Sinkronisasi estrus dan inseminasi buatan (IB) merupakan bioteknologi reproduksi yang dapat membantu manajemen reproduksi di suatu kawasan peternakan dimana peternak dapat mengatur waktu perkawinan yang dapat disesuaikan dengan permintaan pasar. Desa pamulihan Kecamatan Pamulihan Kabupaten Sumedang, memiliki beberapa peternak domba Garut yang memiliki usaha secara komersil dengan jangkauan pemasaran yang cukup luas. Tujuan kegiatan ini yaitu memberikan pengetahuan kepada peternak domba tentang sinkronisasi estrus dan IB untuk dapat diaplikasikan pada sistem pemeliharaan domba yang mereka miliki. Metode yang dilakukan meliputi penyuluhan, diskusi dan tanya jawab, pelatihan dan demonstrasi. Hasil kegiatan menunjukkan adanya peningkatan pengetahuan peternak terhadap materi pelatihan terkait sinkronisasi estrus dan IB yang diketahui dari hasil pre-test dan post-tes, yaitu sebesar 53%. Hasil ini menunjukkan bahwa metode yang dilakukan berupa penyuluhan dan pelatihan cukup efektif memberikan pengetahuan kepada peternak domba di Desa Pamulihan. Disimpulkan bahwa peserta pelatihan mampu menerima ilmu dan keterampilan yang telah diberikan, peserta juga tertarik untuk dapat mengaplikasikan hasil pelatihan terhadap domba yang mereka miliki, namun untuk pelaksanaan secara mandiri masih memerlukan pembinaan dan pendampingan
Vaginal Cytological Changes and Estrus Response Following Estrus Synchronization Using a Double Prostaglandin-F₂α and Gonadotropin Releasing Hormone in Pasundan Heifers Rini Widyastuti; Azis, Azhar Abdul; Setiawan, Rangga; Hilmia, Nena; Lubis, Alkaustariyah; Ridlo, Muhammad Rosyid
Jurnal Veteriner Vol. 27 No. 1 (2026)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19087/

Abstract

Pasundan cattle, an indigenous breed native to West Java, Indonesia, demonstrate significant adaptability and reproductive potential. However, their declining populations pose a threat to genetic conservation. Effective estrus synchronization is essential for enhancing reproductive efficiency and supporting fixed-time artificial insemination (FTAI) programs. Vaginal cytology profiling offers a reliable, noninvasive method for assessing estrus by evaluating the dynamics of epithelial and leukocyte cells, which reflect hormonal fluctuations. This study was aimed to investigate the cytological dynamics of vaginal epithelial, leukocyte cells and estrus response in Pasundan heifers subjected to double injection of prostaglandin F₂α (PGF₂α) and gonadotropin-releasing hormone (GnRH) synchronization. Nine Pasundan heifers, aged 1.5–2.0 years, were treated with PGF₂α with 2.75 μg/kg BW on days 0 and 11, followed by 2 μg/kg BW GnRH on day 12. Vaginal smears were collected daily from days 0 to 15, stained with 5% Giemsa, and examined microscopically to determine the proportions of parabasal, intermediate, superficial, keratinized, and leukocyte cells. The results indicated that the initial cytology showed a luteal phase dominated by parabasal (31.2%) and intermediate (30.6%) cells. Following hormonal treatment, superficial cells peaked at 70.9% on day 13, indicating estrus and estrogen dominance. By days 14–15, keratinized and parabasal cells had increased, confirming post-ovulatory luteal activity. The protocol achieved complete (100%) estrus synchronization among all heifers. It can be concluded that the double PGF₂ α-GnRH protocol effectively synchronized estrus in Pasundan heifers. Vaginal cytology has proven to be a sensitive and practical tool for monitoring reproductive phases, thereby supporting breeding management and conservation of this native genetic resource.
The Effect of Incubation Time on the Quality of Post-Thawed Ram Sexed Sperm Solihati, Nurcholidah; Rasad, Siti Darodjah; Setiawan, Rangga
Livestock and Animal Research Vol 22, No 2 (2024): Livestock and Animal Research
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/lar.v22i2.90802

Abstract

Objective: The sperm sexing using an albumin column requires an incubation process at a certain temperature for sperm to move through the albumin layer. It is thought that the incubation time can influence the quality of the semen resulting from post-thawing sexing. The aim of this research was to determine the effect of incubation time on the quality of sexed sperm post-thawing and to find out the optimum incubation time that provide the best sperm quality.Methods: This research was carried out in a completely randomized design, using three incubation times (T1= 45 minutes, T2= 60 minutes, T3= 75 minutes) with six replications. The parameters consisted of motility, abnormality and acrosome integrity of sexed sperm in the upper and bottom fractions. Data were analyzed using analysis of variance (Anova) and continued with the Duncan test.Results: The results showed that the incubation time had a significant effect on motility, intact acrosome cap and recovery rate, but had no significant effect on abnormality of post thawed sexed sperm. For both the upper and bottom fraction, incubation times of 45 and 60 minutes produced the best motility and recovery rate compared to 75 minutes, but the best intact acrosome cup was obtained from an incubation time of 45 minutes.Conclusions: Based on the research results, it can be concluded that the length of incubation time influences the quality of post thawed ram sexed sperm, and 45 minutes is the optimal incubation time to provide the best quality.
Co-Authors Achmad, Luthfillah Az-zahra Arief Boediono Ayu Putri Noviana, Ninda Azis, Azhar Abdul Citaliani Rostika, Yulia Dwidika, Adyatma Faradana, Najma Rika Fitriani, Resa Gadis Octory Hamdi, Tasya Puteri Aulia Ibrahim, M. Rangga Rangga Katar Laksana, Eka Purwa Lubis, Alkaustariyah Mas Rizky A.A. Syamsunarno Maulana, Gifari Ahmad Mohammad Ghozali, Mohammad Mokhamad Fakhrul Ulum, Mokhamad Nena Hilmia Nurcholidah Solihati NURHAYATI, SITI ZYA Peby Wahyu Purnawan Rasad, Siti Darojah Ridlo, Muhammad Rosyid Rini Widyastuti Roesillah, Diar Santi Nurjanah, Santi Sari, Aprilianna P.Z.N.L. Shabrina, Tiara Siti Darodjah Rasad Soeparna Soeparna, Soeparna Solihati, Nucholidah TAKDIR SAILI Toha, Toha Ulfie, Nasya Shalsabiel Wide Mulyana Winangun, Kikin