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Insecticidal Activities of Ethyl Acetate Extract of Indonesian Mangrove Fungus Emericella nidulans BPPTCC 6038 on Spodoptera litura SILVA ABRAHAM; ADI BASUKRIADI; SUYANTO PAWIROHARSONO; WELLYZAR SJAMSURIDZAL
Microbiology Indonesia Vol. 9 No. 3 (2015): September 2015
Publisher : Indonesian Society for microbiology

Mangrove fungi are known as sources of biological active compounds. The study and the report of secondary metabolites of mangrove fungi as insecticides is very limited in Indonesia. This study assess the insecticidal activities of ethyl acetate extract of Indonesian mangrove fungus Emericella nidulans BPPTCC 6038 against Spodoptera litura (Lepidoptera, Noctuidae) neonate larvae and pupae. The fungus E. nidulans BPPTCC 6038 was isolated from leaves of mangrove Rhizophora mucronata and identified based on ITS rDNA sequence data, with the GenBank accession number KP165435, and confirmed with morphological observation. This fungus strain was grown on malt extract broth for 14 days on rotary shaker at 65 rpm, and incubated at room temperature. Mortalities of S. litura were observed on larvae fed on artificial diet containing ethyl acetate extract of E. nidulans at concentrations of 625 â€“ 5000 ppm. The lethal concentration of the extract which causes 50% mortality of larvae (LC50 value) was 1102.27 ppm. The other effects of fungus extract on S. litura were decrease in growth rate, longer larval period, inhibition on pupal development and absence in adult emergence. The HPLC analysis of extract showed that the crude extract contained three major compounds. This study provides evidence that the extract of E. nidulans possesses insecticidal activities against S. litura.

Influence of Temperature Variations on Growth of Nostoc (Cyanobacteria) HS-5 and HS-20 Isolated from Indonesian Hot Springs NINING BETAWATI PRIHANTINI; CAHYA GUSLYANI; RATNA YUNIATI; WELLYZAR SJAMSURIDZAL
Microbiology Indonesia Vol. 12 No. 2 (2018): June 2018
Publisher : Indonesian Society for microbiology

The research aims to know the effect of variation temperature to the growth of Nostoc HS (Hot Spring)-5 and HS-20. Strain of Nostoc HS-5 was isolated from Ciseeng hot spring which has habitat temperature range of 30-43 Â°C, and Nostoc HS-20 was isolated from Pancar Mountain hot spring which has temperature range of 46-69 Â°C. The research was done by measuring biomass weight and chlorophyll content on day-1, 2, 3, 4, 7, 10, 14, 17, and 21. The temperatures used were 20 Â°C, 35 Â°C, and 50 Â°C. The growth medium used was Bold Basal Medium (BBM) with pH 6.6. Each treatment was made in four replications. Non-parametric statistical analysis used were the Friedman test (a=0.05) and Spearman test (a=0.01).The result showed there were significant differences on the biomass weight of Nostoc HS-5 and HS-20 grown at temperature of 20 Â°C, 35 Â°C, and 50 Â°C. The average amount of biomass highest weight for Nostoc HS-5 and HS-20 occurred in both strains were grown at 35 Â°C.Â Besides that, there was no correlation between the weight of biomass and chlorophyll content of Nostoc HS-5 and HS-20.

Multilocus Sequence Analysis of Cercospora spp. from Different Host Plant Families Yuliarni, Floreta Fiska; Sjamsuridzal, Wellyzar; Hidayat, Iman
Makara Journal of Science Vol. 17, No. 2
Publisher : UI Scholars Hub

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Identification of the genus Cercospora is still complicated due to the host preferences often being used as the main criteria to propose a new name. We determined the relationship between host plants and multilocus sequence variations (ITS rDNA including 5.8S rDNA, elongation factor 1-α, and calmodulin) in Cercospora spp. to investigate the host specificity. We used 53 strains of Cercospora spp. infecting 12 plant families for phylogenetic analysis. The sequences of 23 strains of Cercospora spp. infecting the plant families of Asteraceae, Cucurbitaceae, and Solanaceae were determined in this study. The sequences of 30 strains of Cercospora spp. infecting the plant families of Fabaceae, Amaranthaceae, Apiaceae, Plumbaginaceae, Malvaceae, Cistaceae, Plantaginaceae, Lamiaceae, and Poaceae were obtained from GenBank. The molecular phylogenetic analysis revealed that the majority of Cercospora species lack host specificity, and only C. zinniicola, C. zeina, C. zeae-maydis, C. cocciniae, and C. mikaniicola were found to be host-specific. Closely related species of Cercospora could not be distinguished using molecular analyses of ITS, EF, and CAL gene regions. The topology of the phylogenetic tree based on the CAL gene showed a better topology and Cercospora species separation than the trees developed based on the ITS rDNA region or the EF gene.

PENGEMBANGAN DATABASE MIKROORGANISME INDIGENOS INDONESIA Sjamsuridzal, Wellyzar; Oetari, Ariyanti; Hertono, Gatot F.; Sitaresmi, Sitaresmi
Makara Journal of Science Vol. 10, No. 1
Publisher : UI Scholars Hub

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Development of Database for Indigenous Indonesian Microorganisms. The objective of the research is to create and develop a database of indigenous Indonesian microorganisms based at the University of Indonesia. Development of the database of indigenous Indonesian microorganisms was carried out in several stages, i.e. data identification, database design, programming, data entry, testing and debugging, and repairing and maintenance. Development of the database utilized the licensed software of General Public License (GPL), which include Linux RedHat 9.0 (operating system), Apache ver. 2.20 (web server), MySQL ver. 4.2 (database server), and PHP ver. 4.3 (web interface programming language). The result of this research is a database named UI Bioinfo which has the following facilities: online catalog search for UICC (University of Indonesia Culture Collection) strains collection and sequence homology search utility through BLAST (Basic Local Alignment Search Tool). Integrated information on strains collection was first carried out on the yeast collection. At present, UI Bioinfo contains information for 297 strains that includes isolation data, morphological descriptions, physiology-biochemical characteristics, and images. Moreover it also contains sequence data from the large subunit (LSU) ribosomal RNA gene and the internal transcribed spacer (ITS) regions. UI Bioinfo can be accessed from the following site: http://152.118.162.250/bio/. Future development will be addition of data from the other collections in UICC

PENGEMBANGAN DATABASE MIKROORGANISME INDIGENOS INDONESIA Sjamsuridzal, Wellyzar; Oetari, Ariyanti; Hertono, Gatot F.; Sitaresmi, Sitaresmi
Makara Journal of Science Vol. 10, No. 1
Publisher : UI Scholars Hub

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Development of Database for Indigenous Indonesian Microorganisms. The objective of the research is to create and develop a database of indigenous Indonesian microorganisms based at the University of Indonesia. Development of the database of indigenous Indonesian microorganisms was carried out in several stages, i.e. data identification, database design, programming, data entry, testing and debugging, and repairing and maintenance. Development of the database utilized the licensed software of General Public License (GPL), which include Linux RedHat 9.0 (operating system), Apache ver. 2.20 (web server), MySQL ver. 4.2 (database server), and PHP ver. 4.3 (web interface programming language). The result of this research is a database named UI Bioinfo which has the following facilities: online catalog search for UICC (University of Indonesia Culture Collection) strains collection and sequence homology search utility through BLAST (Basic Local Alignment Search Tool). Integrated information on strains collection was first carried out on the yeast collection. At present, UI Bioinfo contains information for 297 strains that includes isolation data, morphological descriptions, physiology-biochemical characteristics, and images. Moreover it also contains sequence data from the large subunit (LSU) ribosomal RNA gene and the internal transcribed spacer (ITS) regions. UI Bioinfo can be accessed from the following site: http://152.118.162.250/bio/. Future development will be addition of data from the other collections in UICC

PENGEMBANGAN DATABASE MIKROORGANISME INDIGENOS INDONESIA Sjamsuridzal, Wellyzar; Oetari, Ariyanti; Hertono, Gatot F.; Sitaresmi, Sitaresmi
Makara Journal of Science Vol. 10, No. 1
Publisher : UI Scholars Hub

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Development of Database for Indigenous Indonesian Microorganisms. The objective of the research is to create and develop a database of indigenous Indonesian microorganisms based at the University of Indonesia. Development of the database of indigenous Indonesian microorganisms was carried out in several stages, i.e. data identification, database design, programming, data entry, testing and debugging, and repairing and maintenance. Development of the database utilized the licensed software of General Public License (GPL), which include Linux RedHat 9.0 (operating system), Apache ver. 2.20 (web server), MySQL ver. 4.2 (database server), and PHP ver. 4.3 (web interface programming language). The result of this research is a database named UI Bioinfo which has the following facilities: online catalog search for UICC (University of Indonesia Culture Collection) strains collection and sequence homology search utility through BLAST (Basic Local Alignment Search Tool). Integrated information on strains collection was first carried out on the yeast collection. At present, UI Bioinfo contains information for 297 strains that includes isolation data, morphological descriptions, physiology-biochemical characteristics, and images. Moreover it also contains sequence data from the large subunit (LSU) ribosomal RNA gene and the internal transcribed spacer (ITS) regions. UI Bioinfo can be accessed from the following site: http://152.118.162.250/bio/. Future development will be addition of data from the other collections in UICC

AKTIVITAS ANTIFUNGAL MINYAK ATSIRI JINTEN PUTIH TERHADAP Candida parapsilosis SS25, C. orthopsilosis NN14, C. metapsilosis MP27, DAN C. etchellsii MP18 Ridawati, Ridawati; Jenie, Betty Sri Laksmi; Djuwita, Ita; Sjamsuridzal, Wellyzar
Makara Journal of Science Vol. 15, No. 1
Publisher : UI Scholars Hub

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Antifungal Activity of Cumin Oil Against Candida parapsilosis SS25, C. orthopsilosis NN14, C. metapsilosis MP27, and C. etchellsii MP18. Many kinds of spices are used in Indonesia, one of them is white cumin seed. This spice is used not only for cooking, but also for traditional medicine. This study reported of antifungal activity from white cumin’s essential oil. Extraction and identification of Cumin oil were carried out. We obtained 2.5-3.0% of white essential oil which was colorless or light yellow color. GCMS analysis revealed that there were 12 peaks. Based on peak’s intensity the oil were dominated by 4 compound i.e. cuminaldehide (35.44%), ρ-cymene (34.77%), β-pynene (15.08 %) and γ-terpinene (8.15%). Growth inhibition zone determination has been carried out by diffusion disc and direct method against yeast i.e. C. parapsilosis SS25, C. orthopsilosis NN14, C. metapsilosis MP27, and C. etchellsii MP18. The results showed that all of the yeasts were sensitive to cumin oil. The inhibition zone radius were 13.4-16.5 mm. The cumin oil showed the inhibition of yeast growth with MIC values of 0.028%-0.042% and MFC values 0.09%- 0.14%, while nystatin had MIC values 0.40%-0.50% and MFC values 3.0%-4.0%. The activity of cumin oil was very strong as antifungal.

Activities of Cellulolytic, Amylolytic, and Secondary Metabolites from Thermophilic Actinomycetes Collected from the Heated Soil around Cisolok Geyser Sari, Dhian Chitra Ayu Fitria; Ningsih, Fitria; Rachmania, Mazytha Kinanti; Yokota, Akira; Sjamsuridzal, Wellyzar
Makara Journal of Science Vol. 28, No. 4
Publisher : UI Scholars Hub

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This study investigated the antimicrobial activity, cellulolytic, and amylolytic abilities of eight thermophilic Actinomy-cetes isolated from the heated soil around the Cisolok geyser, West Java, Indonesia. Cellulolytic and amylolytic abili-ties were screened for on media by adding 1% (w/v) soluble starch and carboxymethylcellulose (CMC), respectively. Antimicrobial activity was screened for using the agar-plug diffusion method by cultivating strains on various growth media. Growth at various temperatures showed that six strains could grow up to 50 °C. Seven out of eight strains showed amylolytic ability at 40 °C and 45 °C, and only four strains did so at 50 °C. Screening for cellulolytic ability showed that seven strains could hydrolyze CMC at 40 °C, whereas seven and five strains could hydrolyze at 45 °C and 50 °C, respectively. Antibacterial activity showed that strains UICC B-110 and UICC B-118 grown on various media could inhibit the growth of Gram-positive bacteria. The results of the antifungal activity test showed that strain UICC B-118, incubated for seven and 14 days, could inhibit the growth of Candida albicans. Based on 16S rRNA gene se-quence similarity and phylogenetic analyses, strains UICC B-110 and UICC B-118 were identified as Streptomyces glaucescens and Microbispora rosea subsp. rosea, respectively. These results show that thermophilic Actinomycetes from the soil around the Cisolok geyser have the potential as a source of thermostable enzymes and new bioactive compounds with antimicrobial activity.

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