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Enhancing Sheep Rations: Optimal Ammoniated Palm Fronds Utilization with Lerak Fruit (Sapindus Rarak) and Probiotic Supplementation Essy Laura; Mardiati Zain; Fauzia Agustin
Andalasian Livestock Vol. 1 No. 1 (2024): ALive
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/alive.v1.n1.p59-69.2024

Abstract

This study aimed to assess the impact of lerak fruit and probiotic supplementation on varying levels of ammoniated palm fronds in sheep rations, as well as to determine the optimal inclusion rate of ammoniated palm fronds in the presence of lerak fruit and probiotics. Employing an experimental approach, a Latin Square Design was utilized, with four sheep as rows, four periods as columns, and four rations as treatments. Treatments included: A) 50% field grass + concentrate, B) 20% field grass + 30% ammoniated palm fronds + 50% concentrate + lerak fruit + probiotics, C) 10% field grass + 40% ammoniated palm fronds + 50% concentrate + lerak fruit + probiotics, and D) 0% field grass + 50% ammoniated palm fronds + 50% concentrate + lerak fruit + probiotics. Results indicated significant differences (P<0.05) between dry and organic matter intake treatments and body weight gain. However, no significant differences (P>0.05) were observed in the digestibility of dry and organic matter. These findings suggest that supplementation with up to 40% ammoniated palm fronds in sheep rations, probiotics, and lerak fruit yielded comparable digestibility and livestock performance as the control. This study provides valuable insights into optimizing sheep rations with alternative feed ingredients, contributing to enhanced animal nutrition practices.
Fatty acids composition and biohydrogenation reduction agents of tropical forages MALIK MAKMUR; MARDIATI ZAIN; YETTI MARLIDA; KHASRAD KHASRAD; ANURAGA JAYANEGARA
Biodiversitas Journal of Biological Diversity Vol. 20 No. 7 (2019)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d200718

Abstract

Abstract. Makmur M, Zain M, Marlida Y, Khasrad, Jayanegara A. 2019. Fatty acids composition and biohydrogenation reduction agents of tropical forages. Biodiversitas 20: 1917-1922. The study was conducted to determine the composition of fatty acids, measured rumen biohydrogenation reduction agents (total phenols and total tannins) content and selected promising plants in various species of tropical forages. Ten species of tropical forages, namely, Panicum maximum, Cynodon plectostachyus, Pennisetum purpurephoides, Pennisetum purpureum, Brachiaria decumbens, Glyricidia sepium, Calliandra calothyrsus, Stylosanthes guaianensis, Leucaena leucocephala and Indigofera zollingeriana were used in this study. The fatty acids composition (% of total identified fatty acids) which were dominant in grasses were C18: 3n-3 (29%), C16: 0 (28%) and C18: 2n-6 (23%). Whereas in legumes, the significantly higher composition of fatty acids was C18: 3n-3 (42%) followed by C16: 0 (17%) and C18: 2n-6 (17%). The average poly-unsaturated fatty acids (PUFA) composition in grasses was relatively lower (44.6%) than legumes (59%). Likewise the content of total phenols and total tannins (g/100g DM) of grasses (0.91 and 0.41) and legumes (1.72 and 0.70). The selection of the forage plant species was based on the criteria of PUFA composition and biohydrogenation reduction agents using TOPSIS method. The results obtained show that B. decumbens (grass) and I. zollingeriana (legume) had the highest preference value of 0.74 and 0.87, respectively. In conclusion, B. decumbens and I. zollingeriana are forage species that have potential to provide healthier ruminant products.
Effects of supplementation with phosphorus, calcium and manganese during oil palm frond fermentation by Phanerochaete chrysosporium on ligninase enzyme activity Roni Pazla; Novirman Jamarun; Fauzia Agustin; Mardiati Zain; Arief Arief; Nesya Oktiacahyani
Biodiversitas Journal of Biological Diversity Vol. 21 No. 5 (2020)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d210509

Abstract

Abstract. Pazla R, Jamarun N, Agustin F, Zain M, Cahyani NO. 2020. Effects of supplementation with phosphorus, calcium and manganese during oil palm frond fermentation by Phanerochaete chrysosporium on ligninase enzyme activity. Biodiversitas 21: 1833-1838. The objective of this study was to evaluate the effects of supplementation with phosphorus (P) in combination with calcium (Ca) and manganese (Mn) during oil palm frond (OPF) fermentation by Phanerochaete chrysosporium on ligninase enzyme activity and lignin degradation. This study was carried out using a randomized complete design with 3 treatments (addition of P, Ca and Mn) and 5 replicates. The following treatments were performed: T1 (P 1000 + Ca 2000 + Mn 150 ppm), T2 (P 1500 + Ca 2000 + Mn 150 ppm), and T3&nbsp; (P 2000 + Ca 2000 +Mn 150 ppm). The data were subjected to an analysis of variance (ANOVA), and differences between treatment means were tested using Duncan's multiple range test (DMRT). The parameters measured were as follows: lignin peroxidase (LiP) activity (U/mL), manganese peroxidase (MnP) activity (U/mL), crude protein (CP) content (%), crude fiber (CF) content (%) and the decrease in lignin (%). The results revealed a significant increase in LiP activity and CP content and a decrease in the lignin content (p&lt;0.05) by the addition of P in the T3 treatment. However, the treatment nonsignificantly increased (p&gt;0.05) MnP activity and significantly decreased (P&lt;0.05) the CF content. In conclusion, supplementation of the OPF fermentation process with P 2000, Ca 2000, and Mn 150 ppm resulted in the highest ligninase enzyme activity and in decreased lignin content.
Quality evaluation of tithonia (Tithonia diversifolia) with fermentation using Lactobacillus plantarum and Aspergillus ficuum at different incubation times Roni Pazla; Novirman Jamarun; Mardiati Zain; Gusri Yanti; Riki Hiskia Chandra
Biodiversitas Journal of Biological Diversity Vol. 22 No. 9 (2021)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d220940

Abstract

Abstract. Pazla R, Jamarun N, Zain M, Yanti G, Chandra RH. 2021. Quality evaluation of tithonia (Tithonia diversifolia) with fermentation using Lactobacillus plantarum and Aspergillus ficuum at different incubation times. Biodiversitas 22: 3936-3942. This research aimed to evaluate the nutritional quality of tithonia (Tithonia diversifolia) fermented using Lactobacillus plantarum and Aspergillus ficuum with different incubation times on nutritional content, digestibility, phytase enzyme activity, and characteristics of rumen fluid. The research used an experimental method with a factorial completely randomized design to evaluate the nutritional content and phytase enzyme activity after fermentation (stage 1). A randomized block design was used to evaluate in vitro digestibility and rumen fluid characteristics (stage 2). For factorial completely randomized design, factor A is the type of microbe (L. plantarum and A. ficuum), then factor B is the incubation time (3,5,7 days). Parameters observed were the nutritional content of dry matter (DM), organic matter (OM), crude protein (CP), crude fiber (CF), and phytase enzyme activity. For the Randomized Block Design, the research treatments were A = A. ficuum + 5 days of incubation, B = A. ficuum + 7 days of incubation, C = L. plantarum + 3 days of incubation, D = L. plantarum + 5 days of incubation. Parameters measured were the digestibility of dry matter(DMD), organic matter (OMD), crude protein (CPD), crude fiber (CFD), rumen pH, VFA production, and NH3 rumen fluid. The results showed that there was an interaction between the type of microbe and incubation time. The treatment had a significantly different effect (P&lt;0.05) on the content of OM, CP, CF and phytase enzyme activity, but no interaction with the content of DM treatment. In the digestibility, the results showed that the effect was not significantly different (P&gt;0.05) on DMD, OMD, CPD but had a significant effect (P&lt;0.05) on CFD. The treatments also had no significant effect (P&gt;0.05) on VFA but were significantly different (P&lt;0.05) on NH3. From this study, it can be concluded that fermented tithonia using A. ficuum with an incubation period of 7 days could improve the quality of tithonia. It can be seen from the content of CP (31.02%), CF (16.52%), phytase enzyme activity (37.36 U/ml), DMD (66.86%), OMD (67.36%), CFD (81.01%), CPD (70.37%), VFA production 135 mM and NH3 concentration 14.31 mg/100 ml, and pH value 6.72 which is suitable for rumen microbial growth.
Impact of protein source from legume forages on lipid regulation and growth on native ram lambs reared extensively in the south coast of West Java, Indonesia UJANG HIDAYAT TANUWIRIA; ANDI MUSHAWWIR; MARDIATI ZAIN; DESPAL DESPAL
Biodiversitas Journal of Biological Diversity Vol. 24 No. 7 (2023)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d240763

Abstract

Abstract. Tanuwiria UH, Mushawwir A, Zain M, Despal D. 2023. Impact of protein source from legume forages on lipid regulation and growth on native ram lambs reared extensively in the south coast of West Java, Indonesia. Biodiversitas 24: 4183-4192. This investigation was carried out in three different locations to examine the impact of legume forage as a protein source on lipid regulation and growth. This study used 120 rams, consisting of 40 rams in each location (Garut, Cianjur, Sukabumi) spread along the South Coast of West Java. Physical environmental conditions were recorded during the study. Ram lambs at each location were divided into four groups; one group only received a basal diet (Setaria/Setaria sphacelata), and the other three groups were each given additional legumes (calliandra/Calliandra calothyrsus, lamtoro/Leucaena leucocephala, and moringa/Moringa oleifera). Blood samples were collected every two weeks during the 16 weeks experiment. The blood sampling was handled based on standard procedures to prevent sample damage, and the body weight was measured every two weeks. Sample analysis related to lipid regulation and creatine kinase activity was measured using spectrophotometric techniques according to the instructions of the Kit Randox protocol and analysis of blood plasma fatty acids were analyzed by gas chromatography. The research results show that legumes as a protein source can regulate through molecular signaling to decrease lipid levels, saturated fatty acids, and lipid transport in the extracellular fluid, but increase unsaturated fatty acid and the growth rate of ram lambs. Based on the results of the current study, it can be a strong basis for the development of nutrigenomic aspects of protein for small ruminants, accompanied by studies of the ages of different ruminant samples.