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Purine derivatives excretion and estimation of microbial protein supply on sheep receiving different protein suplements Natsir, Asmuddin
Indonesian Journal of Animal and Veterinary Sciences Vol 12, No 3 (2007)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (59.38 KB) | DOI: 10.14334/jitv.v12i3.483

Abstract

The urinary excretion of purine derivative (PD), which consists of allantoin, uric acid, xanthine and hypoxanthine, was used to estimate Microbial Nitrogen (MN) supply on sheep given oaten hay as basal diet supplemented either with faba beans (faba) or barley fortified with urea (barley-urea). Three growing wethers, with average body weight of 43 ± 2.29 kg, were randomly assigned to one of three experimental diets, i.e. oaten hay without supplement (C), diet C + barley-urea (CB), and diet C + faba (CF), according to latin square design (3 x 3). Results of the experiment indicated that PD excretion for C was lower (P = 0.07) than that of CB and CF (2.84 vs 6.08, 7.67 mmol/d). Absorbed PD and estimation of MN supply for C was less (P = 0.07) than those of CB and CF, namely 1.26 vs 6.64, 8.80 mmol/d and 0.91 vs 4.8, 6.30 g N/d respectively. However, the efficiency of MN synthesis was similar, whether the calculation was based on digestible organic matter fermented in the rumen, DOMR (g MN/kg DOMR, P = 0.20) or based on N intake (g MN/g N intake, P = 0.40). In conclusion, supplementation of oaten hay with different protein supplements may improve the estimation of microbial N supply in the growing sheep but both supplements gave similar results on the estimation of MN supply. Key Words: Purine Derivative, Microbial Protein, Protein Supplement, Sheep
The effects of microwave radiation on rumen degradation characteristics of barley straw cut at two different stages of maturity Natsir, Asmuddin
Indonesian Journal of Animal and Veterinary Sciences Vol 12, No 2 (2007)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (124.149 KB) | DOI: 10.14334/jitv.v12i2.548

Abstract

A common approach for improving the nutritive value of low quality roughages and crop by-products is by pre-treatment or processing either physical, chemical, or biological treatments. Microwave radiation is one type of physical treatment that could be used to treat low quality roughages. Research was carried out to investigate  the effects of microwave radiation on the rumen degradation characteristics of barley straw obtained from two different stages of maturity. The experiment was run factorially based on completely randomized block design. The first factor was stage of maturity, straw cut during the soft elongation time (C1) and during the harvest time (C2). The second factor was levels of microwave radiation times (MWRT) (T0 = control, without MWR; T1 = MWR for 1 minute, T2 = MWR for 2 minutes). The results indicated nutritive values of barley straw obtained from C1 sampling time were significantly better than that obtained from the C2 sampling time in terms of a higher rumen degradation rate and a much greater total potential rumen degradability. In contrast, MWR did not have significant effects on the rate of degradation and total potential degradability of straw in the rumen. Key Words: Microwave Radiation, Barley Straw, Rumen Degradability
Rumen microbial protein supply as estimated from purine derivative excretion on sheep receiving faba beans (vicia faba) as supplement delivered at different feeding frequencies Natsir, Asmuddin
Indonesian Journal of Animal and Veterinary Sciences Vol 13, No 2 (2008)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (66.582 KB) | DOI: 10.14334/jitv.v13i2.602

Abstract

Rapid and extensive degradation of faba beans (Vicia faba) by ruminal microbes can result in substantial and undesirable N loss from the rumen. The purpose of this study was to test the hypothesis that offering faba beans as a supplement more than once a day to sheep receiving a combination of oaten chaff and lucerne chaff as a basal diet will increase microbial protein supply to the intestines. The experiment was conducted in a Latin square design (4 x 4) using four mature merino sheep. The treatments were: T0 = basal diet ad libitum + nil supplements, T1 = T0 + faba beans (FB) fed once daily, T2 = T0 + FB fed twice daily, T3 = T0 + FB fed 8 times daily. The basal diet was given once per day at 09:00 in the morning while FB were given at the rate of approximately 0.5% of live body weight and delivered according to the treatment protocol. Urinary excretion of purine derivative (PD) was used to estimate microbial protein supply. The results indicated that even though treatment statistically had no effects on total urine output, PD excretion in the urine, PD absorbed, estimated microbial N supply, and the efficiency of rumen microbial protein synthesis, provision of supplement to sheep numerically improved microbial N supply by 92% compared to that of control group. However, there were no differences within the supplemented group. Therefore, it is concluded that feeding faba beans more than once a day was unnecessary. Key Words: Purine Derivatives, Microbial Protein Synthesis, Sheep
The Effect of addition different levels of katuk leaf meal in the ration on cholesterol, triglyceride, LDL, and HDL of broiler blood Kamalia, .; Mujnisa, Andi; Natsir, Asmuddin
Buletin Nutrisi dan Makanan Ternak Vol 10, No 1 (2014)
Publisher : Universitas Hasanuddin

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Abstract

The aims experiment were to study the effect of adding different levels of katuk meal on the levels of cholesterol,triglycerides,LDL,and HDL in the blood of broiler chiken. Sixty four (64) treatment diets according to completely randomized design.  The treatment diets were :P0 = contol, P1 = control + 1 % katuk leaf meal, P2 = Control + 2% katuk leaf meal, P3 = control + 3 % katuk leaf meal.  The replication for each treatment was four giving total number of experimental diet was 16.  Control ration consisted of brn, yellow cron, coconut oil, shrimp waste, fish meal, tofu dregs, soy bean.  During the experiment broiler chiken ware given a free access to drinking water.  The resuls of study indicated that addition of katuk leaf meal into the diet did not affect concentration of cholesterol, triglycerides, and LDL in the blood of broiler.  But significantly increased the concentration of blood HDL but had no effects on concenetration of blood cholesterol, blood triglycerides, and blood LDL of broiler.
Preparation of Phosphate Liquid and Urea Molasses Minerals Liquid (UMML) As a Precursor of Rumen Biofermentation Syahrir, Syahriani; Natsir, Asmuddin; Mide, Muhammad Zain; Islamiyati, Rohmiyatul; Asrianie, Ani
Buletin Nutrisi dan Makanan Ternak Vol 9, No 1 (2013)
Publisher : Universitas Hasanuddin

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Abstract

Urea Molasses Minerals Liquid (UMML) may be a precursor of rumen biofermentation. UMML can provide slow-release nitrogen, minerals and readily available carbohydrate (RAC), but require special studies, especially in determining the type and preparation of constituent materials, especially the provision of water-soluble phosphate minerals. The purpose of this study is to produce a formula of UMML then be used as a precursor of rumen biofermentation. Some of the treatment of super phosphate (SP36) was performed to obtain a preparation method produces dissolved  phosphate  concentrations  are high. Preparation  that  the  highest levels of dissolved phosphate is mixed of super phosphate, organic acids and urea solution, then boiled for 5 minutes. Urea Mineral Molasses liqiud mixed from materials Ca(urea)4Cl2 phosphate solution, saturated NaCl solution and molasses.
STUDY ON BUFFALO FATTENING USING AGRICULTURAL WASTE BASED FEEDING AT THE TANA TORAJA DISTRICT Nurhayu, A.; Sariubang, Matheus; Ishak, Andi Baso L.; Natsir, Asmuddin
Proceeding Buffalo International Conference 2013
Publisher : Proceeding Buffalo International Conference

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Abstract

The study was conducted in the Tiromanda village, South Makale sub district, Tana Toraja district. The aim of the study was to measure the effect of providing agricultural waste based feeding to buffalo fattening. Fifteen male buffaloes, aged between 3 - 5 years with body weight ranging between 200 and 250 kg, were randomly assigned to one of three experimental diets, namely (A) 12 kg fermented rice straw + 2.5 kg concentrates; (B) 10 kg fermented rice straw + 5 kg concentrate and (C)control diet (ration typically used by the farmer) according to completely randomised design. Formulation of feed was based on agrricultural waste locally available, i.e. rice straw and concentrates. Determination of body weight was carried out every month for four months. The results showed that the average daily body weight gain (ADG) of buffaloes in the treatment B was the highest compared to that of the treatment A and C, namely 0.8 kg/head/day; 0.6 kg/head /day and 0.3 kg/head/day, respectively. Feed conversion ratio for each treatment was 31.6 (A), 14.87 (B), and 41.3(C). In conclusion, provision of feed based on agricultural waste such as rice straw plus concentrates increased body weight gain in fattening buffaloes.
PENGARUH PENAMBAHAN BERBAGAI LEVEL TEPUNG DAUN KATUK (Sauropus Androgynus) TERHADAP KADAR KOLESTEROL, TRIGLISERIDA, LDL DAN HDL DARAH BROILER Kamalia, .; Mujnisa, Andi; Natsir, Asmuddin
Buletin Nutrisi dan Makanan Ternak Vol 10, No 1 (2014)
Publisher : Buletin Nutrisi dan Makanan Ternak

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Abstract

Percobaan ini bertujuan untuk mengetahui pengaruh penambahan berbagai tingkat daun katuk terhadap  kadar kolesterol, trigliserida, LDL, dan HDL dalam darah ayam broiler. Enam puluh empat (64) ekor  DOC berjenis kelamin campur, dengan metode rancangan acak lengkap. Ransum perlakuan adalah: P0 = contol, P1 = kontrol + 1% tepung daun katuk, P2 = Kontrol + 2% daun katuk makan,     P3 = kontrol + 3% tepung daun katuk. Perlakuan berjumlah 4, sehingga total perlakuannya menjadi 16. Ransum kontrol terdiri dari jagung kuning, minyak kelapa, limbah udang, tepung ikan, ampas tahu, kacang kedelai. Selama percobaan ayam broiler diberi pakan dan minum secara adlibitung. penelitian menunjukkan bahwa penambahan tepung daun katuk ke dalam pakan tidak mempengaruhi konsentrasi kolesterol, trigliserida, dan LDL dalam darah ayam broiler. Tetapi secara signifikan meningkatkan konsentrasi HDL darah tetapi tidak memiliki efek pada konsentrasi kolesterol darah, trigliserida darah, dan LDL darah ayam broiler. Kata kunci: Tepung Daun Katuk, Kolesterol, HDL
PREPARASI LARUTAN FOSFAT DAN UREA MINERAL MOLASES LIQUID (UMML) SEBAGAI PENYEDIA PREKURSOR BIOFERMENTASI RUMEN Syahrir, Syahriani; Natsir, Asmuddin; Mide, Muhammad Zain; Islamiati, Rohmiyatul; Asriani, Anie
Buletin Nutrisi dan Makanan Ternak Vol 9, No 1 (2013)
Publisher : Buletin Nutrisi dan Makanan Ternak

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Abstract

Urea Mineral Molases Liquid (UMML) dapat menjadi prekursor biofermentasi dalam sistem rumen. UMML dapat menyediakan nitrogen lepas lambat, mineral larut air dan readily available carbohydrate (RAC),  namun memerlukan kajian khusus, terutama pada penentuan jenis dan preparasi bahan penyusunnya, khususnya penyediaan mineral fosfat larut air.  Tujuan penelitian ini adalah menghasilkan formula UMML yang selanjutnya akan digunakan sebagai prekursor biofermentasi rumen.  Beberapa perlakuan terhadap super fosfat (SP36) dilakukan untuk mendapatkan metode preparasi yang menghasilkan fosfat terlarut dengan konsentrasi yang tinggi. Preparasi yang menghasilkan kadar fosfat terlarut yang tertinggi adalah super fosfat yang di tambah asam organik dan larutan urea lalu dididihkan selama 5 menit. Urea Mineral Molases Liqiud diramu dari bahan Larutan Ca(Urea)4Cl2 + Larutan fosfat + Larutan NaCl jenuh + Molases. Kata Kunci:  Preparasi, Larutan fosfat, Urea Mineral Molases Liquid.
Eplorig Dair Farer’s Kowledge ad Practices of Maure Maageet i Enrekang Regency Baba, Syahdar; Natsir, Asmuddin; Fatma, Fatma; Idrus, Izdin
Proceeding INTERNATIONAL SEMINAR IMPROVING TROPICAL ANIMAL PRODUCTION FOR FOOD SECURITY PROCEEDING INTERNATIONAL SEMINAR
Publisher : Proceeding INTERNATIONAL SEMINAR IMPROVING TROPICAL ANIMAL PRODUCTION FOR FOOD SECURITY

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Abstract

The consequency of the increasing of dairy cattle population in Enrekang is environmentalpollutio fo aue.  The ojetie of this eseah as to eploe dai fae’s koledge adpractices of manure management in Enrekang Regency.  The design of research was survey with 79respondents from a total of 460 dairy farmers that divided by 65 respondents was small scale (1-3heads each farmer) and 14 respondents was medium scale (more than 3 heads each farmers).  Datawere obtained through interview and observations using questionnaire with open and closequestion complementa.  Fatos of fae’s koledge ee ethod ad adatage of aueaageet, eooi alue of aue,   fae’s eed ad adatage of ogai fetilize. Fatos of fae’s paties ee leaig itesit of a ad dai attle, aue aagementand the use of manure.  The data were analyzed with descriptive statistic using frequencydistiutio ad hi suae.  Fae’s koledge of aue aageet as the sae eteeediu ad sall sale χ > 5%.  The koledge of eooi alue, advantage of organic fertilizerad aue aageet as high hile ethod of aue aageet ad fae’s eed oforganic fertilizer was medium.  Cleaning intensity of barn and dairy cattle was high while practice ofmanure handling and manure using was law (rarely).Key Words: Knowledge, Dairy Farmer, Manure
Pengaruh Variasi Media terhadap Aktivitas Fitase Burkholderia Sp. Strain HF. 7 Hafsan, Hafsan; Maslan, Muhammad; Masri, Mashuri; Agustina, Laily; Natsir, Asmuddin; Ahmad, Ahyar
bionature Vol 20, No 1 (2019): April
Publisher : Fakultas MIPA UNM

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (385.309 KB) | DOI: 10.35580/bionature.v20i1.9759

Abstract

Abstract.  Cereals and legumes used as animal feed containing phytic acid which cannot be absorbed by the digestive tract of monogastric animals because phytic acid (C6H18O24P6) is an antinutrient that binds approximately 80% P in feed, also binds to proteins, vitamins and minerals (Mg++, Fe++, Zn++, Mn++, Ca++). Then, one option to overcome this problem is the application of phytase enzymes from various sources, including those produced by bacteria. Phytase enzymes can hydrolyse phytic acid in the feed. This study was aimed to determine the growth phase of Burkholderia sp. HF.7 and optimisation of production of phytase activity from variations of Phytate Production Media (PPM) media. This research used as a descriptive approach. The study design used a completely randomised design (CRD) with a factorial pattern consisting of 2 factors, each variation in phytate sources: calcium (Ca) phytate, rice bran, corn bran, and soybeans. Nitrogen source: (NH4) 2SO4, yeast extract, and peptone. Burkholderia sp. HF.7 Growth phase as a standard for phytase production is the 62-hour stationary phase with an OD value of 2.060 log/cell. The optimum phytase production in the variation of PPM media is soybean-peptone with a protein content value of 46.5 mg/mL and an activity value of 8.20 U/mL under conditions of pH 7 with incubation of 37oC for 62 hours. So, phytase activity produced by PPM of cereal crops has a higher current value compared to PPM Ca-phytate media with low activity value.Keywords: Burkholderia, Phytate, Phytase, enzymes.