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All Journal Jurnal Ilmu Kedokteran (Journal of Medical Science) Jurnal Online Mahasiswa (JOM) Bidang Kedokteran Jurnal Pengabdian UntukMu NegeRI Buletin Farmatera INDONESIAN JOURNAL OF MEDICAL LABORATORY SCIENCE AND TECHNOLOGY Narra J
Amru Sofian
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Religion Biochemistry, Genetics & Molecular Biology Computer Science & IT Dentistry Economics, Econometrics & Finance Education Health Professions Immunology & microbiology Law, Crime, Criminology & Criminal Justice Medicine & Pharmacology Public Health Social Sciences

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Genetic diversity in the E6 and E7 gene of human papillomavirus type 16 among cervical cancer patients in Riau Province, Indonesia Savira, Maya; Rachmadina, Rachmadina; Mahargyarani, Azza Enggar; Admiral, Muhammad Zhafran; S, Donel; Sofian, Amru; Razali, Renady Reza; Ilmiah, Zidny; Farniga, Analdi
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 8 No 1 (2026): Integration of Molecular Approaches in Addressing Drug Resistance and Changing Gl
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/ijmlst.v8i1.6652

Abstract

Cervical cancer mostly occurs due to persistent high-risk human papillomavirus (HPV), with type 16 being the most frequent. In Indonesia, cervical cancer ranks second in mortality, with a fatality rate of 57%. The E6 and E7 genes of HPV-16 play crucial roles in the virus’s oncogenic transformation, leading to cervical cancer. This study was conducted to determine the prevalence and genetic diversity of the E6 and E7 genes of HPV-16 in Riau Province, Indonesia. There were 37 HPV-positive samples analyzed using the MY09/11 primers. This was followed by the amplification of the E6 and E7 genes. Nineteen samples were detected as HPV-16, thirteen of which were coinfected with HPV-18. Seven E6 and E7 sequences were aligned compared with the reference sequence NC_001526.4. The most common nucleotide changes in the E6 gene, 7318A>G, was detected in 30.7% of samples, leading to an amino acid change from 65N>S. Three nucleotide changes were identified in the E7 gene of sample 89: two synonymous (7831T>C, 7837T>G) and one non-synonymous (7989A>G), resulting in an amino acid change of 29N>S. The most frequent E7 nucleotide change, 7708G>A, was found in 80% of samples. Phylogenetic analysis revealed that HPV-16 isolates from Riau have close kinship with the European lineage, with 57.1% (E6) and 85.7% (E7). In conclusion, the incidence of cervical cancer in Riau Province caused by HPV-16 is 52.8% and 7318A>G and 7708G>A are the most common genetic diversity. Furthermore, the majority of HPV-16 isolates in Riau Province show close kinship with the European lineage.
Co-Authors Ade, Wiwit Admiral, Muhammad Z. Admiral, Muhammad Zhafran Awanda Herman Azizah " Bayu Fajar Pratama Dhini Aiyuli Novri Dhini Aiyulie Novri Dimas Pramita Nugraha Donel S Dyah Astrid Astrid Ega Rahman Evsya Eka Bebasari Fadilla Rizki Putri Farniga, Analdi Farniga, Arnaldi Fatmawati " Fifia Chandra Firdaus Gerry Pratama Hendra Perkasa Ika A. Rini, Ika A. Ilmiah, Zidny Kemal, Rahmat A. Mahargyarani, Azza E. Mahargyarani, Azza Enggar Mardhiah Gaffar Maya Savira Monaliza " Nursida Islami, Nursida Ovinitha, Sarah Putra, Andani E. Putri Deas H Rachmadina, Rachmadina Rahmadani, Fetty Try Razali, Renady Reza Razali, Renardy R. Riki Sukiandra Riyan M Darundryo S, Donel Sindy Oktaviani Suyanto " Trigen Yulis Rahmat Yulis Tryanda Ferdyansyah Vicennia Serly Winarto Winarto Wiwit Ade Wiwit Ade Fidiawati Yanti Ernalia