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All Journal Jurnal Ilmu Ternak Jurnal Biodjati Animal Production : Indonesian Journal of Animal Production Jurnal Nukleus Peternakan Jurnal Produksi Ternak Terapan (JPTT) Jurnal Kedokteran Hewan Jurnal Agripet Animal Production
Nurcholidah Solihati
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Economics, Econometrics & Finance Environmental Science Immunology & microbiology Medicine & Pharmacology Veterinary

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PENGARUH KADAR RESIQUIMOD (R848) TERHADAP PERSENTASE SPERMATOZOA X DAN Y PADA SEMEN KAMBING PERANAKAN ETAWAH NURHAYATI, SITI ZYA; Solihati, Nurcholidah; Setiawan, Rangga
Jurnal Produksi Ternak Terapan Vol 6, No 1 (2025): Volume 6 Nomor 1 Maret 2025
Publisher : Faculty of Animal Husbandry, Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/jptt.v6i1.55886

Abstract

The swim up method is a method of selecting X and Y spermatozoa. This study aims to determine how resiquimod (R848) levels affect the percentage of X spermatozoa and Y spermatozoa in the semen of Etawah crossbreed goats (PE) resulting from sexing using the swim up method. This research was carried out from 1 - 30 January 2024 with the research object being male PE goats with a lifespan of 4 years. The research results were analyzed using variance, followed by Duncan's multiple range test to determine differences between treatments. In this study, four treatments were given, namely  (without R848),  (R484 0.3 μM),   (R484 0.6 μM), and  (R848 0.9 μM). Each treatment was repeated six times. The results of this study showed that the use of resiquimod (R848) did not have a significant difference in the percentage of X and Y spermatozoa. It can be concluded that it had no effect on the percentage of PE goat spermatozoa in the lower and upper layers.Keywords: Resiquimod (R848), Percentage of Spermatozoa, Swim up method, Goat PE
Performance and Reproductive Ripitability Value of Holstein Friesian Dairy Cattle in South Garut Farmers Cooperative Nuraeni, Ina; Indrijani, Heni; Solihati, Nurcholidah
Jurnal Ilmu Ternak Universitas Padjadjaran Vol 25, No 1 (2025): Volume 25 No. 1 Maret 2025
Publisher : Fakultas Peternakan, Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/jit.v25i1.45802

Abstract

The purpose of this study was to determine the performance and repeatability values of S/C, DO, and CI in the South Garut Farmers Cooperative. Reproduction data used were individual reproduction records of 162 Friesian Holstein dairy cows. The results showed that the average reproductive performance for S/C for parity 1 was 2.20 ± 0.797, parity 2 was 2.40 ± 0.974, parity 3 was 2.28 ± 0.947, parity 4 was 2.22 ± 0.965, and the total value of S/C was 2.28 ± 0.921. The DO value for parity 1 was 159.50 ± 80.838, parity 2 was 150.43 ± 73.874, parity 3 was 142.29 ± 56.689 days, parity 4 was 133.79 ± 42.678 days, and the total DO was 146, 50 ± 67.267 days. The CI value for parity 1 was 437.91 ± 80.144 days, parity 2 was 430.20 ± 75.211 days, parity 3 was 423.31 ± 57 days, parity 4 was 414.59 ± 42.470 days, and the total CI was 426, 5 ± 67.355 days. The repeatability value at KPGS for S/C was 0.17 ± 0.033, DO was 0.1 ± 0.040, and CI was 0.097 ± 0.040. These three reproductive properties show repeatability values in the low category
THE RELATIONSHIP BETWEEN BETA-LACTOGLOBULIN POLYMORPHISM TO CLINICAL MASTITIS AND REPRODUCTIVE DISORDERS IN FRIESIAN COWS AT PANGALENGAN AREA Romina, Saskia Putri; Septiyani, Septiyani; Solihati, Nurcholidah
Jurnal Ilmu Ternak Universitas Padjadjaran Vol 25, No 1 (2025): Volume 25 No. 1 Maret 2025
Publisher : Fakultas Peternakan, Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/jit.v25i1.59772

Abstract

Beta-lactoglobulin genetics is one of the whey proteins in cow's milk that can influence improvements in milk composition, production, and component levels. Reproductive disorders and clinical mastitis in livestock are often related to genetic, hormonal, and environmental factors. Research on the relationship between beta-lactoglobulin and cow reproduction is still very limited. Therefore, the aim of this study is to determine the relationship between beta-lactoglobulin genotype and clinical mastitis as well as reproductive disorders (dystocia, retained placenta, and endometritis). This research utilizes secondary data obtained from the KPBS Pangalengan headquarters, consisting of genotype examination results of betalactoglobulin and medical records of cows experiencing clinical mastitis and reproductive disorders. A total of 113 samples were collected. The data were analyzed using quantitative methods, followed by ChiSquare tests using Microsoft Excel and SPSS version 25.0 for data analysis. The results showed that the beta-lactoglobulin genotype was not associated with the incidence of clinical mastitis (p=0.509) or reproductive disorders such as dystocia (p=0.789), retained placenta (p=0.193), and endometritis (p=0.685). All test results indicated significance values ≥0.05. The conclusion of this study is that polymorphism beta-lactoglobulin does not have a significant relationship with clinical mastitis and reproductive disorders occurring in Friesian Holstein cows at Pangalengan area. Keywords: beta-lactoglobulin, clinical mastitis, dystocia, endometritis, Friesian Holstein cow, retained placenta.
PENGARUH PENAMBAHAN SARI KURMA DALAM PENGENCER TRIS KUNING TELUR TERHADAP VIABILITAS DAN MOTILITAS SPERMA PADA SEMEN CAIR DINGIN DOMBA LOKAL ALI, SYAHRIR FAJRUL; Solihati, Nurcholidah
Jurnal Produksi Ternak Terapan Vol 6, No 2 (2025): Volume 6 Nomor 2 Juli 2025
Publisher : Faculty of Animal Husbandry, Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/jptt.v6i2.47169

Abstract

Domba merupakan ruminansia kecil penghasil daging, susu, kulit dan produk lainnya untuk memenuhi kebutuhan hidup manusia. Untuk mengoptimalisasikan populasi, perlu terjadinya reproduksi yang dapat dilakukan melalui kawin alami dan Inseminasi Buatan, melalui teknologi IB seekor pejantan dapat mengawini beratus-ratus betina. Penelitian telah dilaksanakan selama 4 minggu, dimulai sejak tanggal 1 Maret 2022 sampai dengan 29 Maret 2022. Penelitian bertempat di Lab. reproduksi Fakultas Peternakan Universitas Padjadajaran. Penelitian menggunakan domba lokal jantan dengan umur 3 tahun. Rancangan yang digunakan Rancangan Acak Lengkap, dengan 5 perlakuan dan 4 ulangan, yaitu  chilledsemen  ditambahkan dengan 0,25% sari kurma (P1), chilledsemen ditambahkan dengan 0,5% sari kurma (P2), chilledsemen  ditambahkan dengan 0,75% (P3), dan chilledsemen ditambahkan dengan 1% sari kurma (P4). Hasil penelitian menunjukkan bahwa keempat perlakuan berpengaruh tidak nyata terhadap motilitas dan viabilitas terhadap chilledsemen yang telah di campurkan oleh sari kurma. Dapat disimpulkan bahwa penambahan sari kurma 0,75% terhadap chilledsemen memiliki hasil yang terbaik di banding dengan 3 perlakuan lainnya secara kuantitatif memiliki hasil yang mendekati hasil kontrol positif (P1), sehingga sari kurma berpotensin untuk menaiki nilai motilitas dan viabilitas.  
Effect of PMSG administration in combination with vaginal sponge on estrous occurrence and litter size of Javanese sheep Setiawan, Rangga; Rasad, Siti Darodjah; Solihati, Nurcholidah; Widyastuti, Rini; Soeparna, Soeparna
Jurnal Kedokteran Hewan Vol 11, No 4 (2017): December
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v11i4.4349

Abstract

AbstractThe use of vaginal sponge in estrous synchronization has been a popular method to uniform estrous cycle of small ruminants. In order to increase the litter size, the use of the sponge is combined with PMSG. Therefore the objective of this study was to evaluate the effect of PMSG in combination with vaginal sponge on estrous response and litter size of Javanese local sheep. The study was conducted in Faculty of Animal Husbandry, Padjadjaran University, Indonesia with 32 non-pregnant local sheep. All these sheep was divided into four groups of treatment, i.e: 1) group of sheep was subjected to vaginal sponge (VS) treatment containing 30 mg Medroxyprogesterone acetat (MAP) inserted in the vagina for 14 days; 2) group of sheep was subjected to the 30 mg MAP in VS plus 300 IU PMSG injection -1 d before VS removal; 3) group of sheep was subjected to the 30 mg MAP in VS plus 300 IU PMSG injection at the day of VS removal; 4) group of sheep was subjected to the 30 mg MAP in VS plus 300 IU PMSG injection +1 d after VS removal. The result showed that all treatment groups had 100% of estrous response. However, the occurrence of estrous time varied among group after VS removal. Group 1 (G1) and G4 tend to have estrous occurrence at day 3 after VS removal by 55% and 75% response, respectively. Whilst, G2 and G3 tend to have estrous occurrence respectively at day 1 (62,5%) and day 2 (50%) after VS removal. A 100% of pregnancy rate occurs in G2 and G4, while G1 and G3 have a 88,9% and 87,5%, respectively. No significant effects of the treatments on the ewes litter size. In conclusion, the use of PMSG administration 24 h prior, after, or at sponge withdrawal increase a tighter synchrony of oestrous and pregnancy rate, but has no effect on the increasing of litter size in Javanese local sheep.Keywords: Vaginal sponge, PMSG, estrous response, pregnancy rate, litter size, sheep
Effect of Skim Nanoparticles on the Motility and Kinematics of Simmental Cattle Frozen Semen Anggraeni, Fitri Dian; Rasad, Siti Darodjah; Solihati, Nurcholidah
Jurnal Agripet Vol 24, No 2 (2024): Volume 24, No. 2, October 2024
Publisher : Faculty of Agriculture

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17969/agripet.v24i2.31736

Abstract

Nanotechnology has positively impacted the development of extenders used in the cryopreservation of spermatozoa in livestock. The inclusion of nano skim in the extender is expected to preserve semen quality after thawing. This study aimed to determine the optimal concentration of nano skim. Various concentrations of nano skim (0%, 1.66%, 3.33%, 5%, 6.66%, 8.33%, and 10%) with the addition of 10% nano egg yolk in each extender were compared. Fresh semen samples were collected from three three-year-old Simmental bulls using an artificial vagina. The study parameters included motility (total motility, progressive motility) and kinematics (velocity curve linear (VCL), velocity straight linear (VSL), velocity average pathway (VAP), amplitude lateral head (ALH), beat cross frequency (BCF), straightness (STR), linearity (LIN), and wobble (WOB). A completely randomized design was employed, and data were analyzed using analysis of variance (ANOVA). Fresh semen dilution results indicated that only the control group (0%) and the nano skim group with concentrations of 6.66% and 8.33% were eligible for further processing into frozen semen. The results showed no significant difference between the three treatment groups on sperm motility and kinematics of Simmental cattle after thawing (p0.05). The control group exhibited the highest percentage of total motility, VCL, VAP, and ALH values, while the 6.66% nano skim group outperformed the 8.33% nano skim group. The highest percentage of progressive motility and VSL value was observed in the 6.66% nano-skim group compared to the 8.33% nano-skim and control groups. The highest values of BCF, WOB, LIN, and STR was observed in the nano-skim group at 8.33% compared to the nano-skim group at 6.66% and control. The study concluded that 6.66% nano skim was the optimal concentration to maintain the quality of frozen semen of Simmental cattle.
Pengaruh Kadar Gliserol Terhadap Kualitas Semen Domba Lokal Solihati, Nurcholidah; Rasad, Siti Darojah; Setiawan, Rangga; Nurjanah, Santi
Jurnal Biodjati Vol 3 No 1 (2018): May
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v3i1.2357

Abstract

Pengembangan Domba Lokal melalui program Inseminasi Buatan memerlukan informasi mengenai kualitas semen, khususnya semen beku yang dalam pembuatannya memerlukan gliserol sebagai krioprotektan dengan kadar yang tepat. Penelitian ini bertujuan untuk mengetahui  kualitas semen Domba Lokal dan mengetahui level gliserol yang menghasilkan kualitas semen post-thawing yang paling optimal. Penelitian dilakukan dengan menggunakan rancangan acak lengkap (RAL) dengan empat perlakuan level gliserol (4%, 5%, 6% dan 7%) dan 14 kali ulangan. Parameter yang diamati meliputi motilitas, abnormalitas dan recovery rate. Data yang diperoleh dianalisis menggunakan analisis ragam (ANAVA) dan perbedaan antar perlakuan diuji menggunakan Uji lanjut Duncan. Hasil penelitian menunjukkan bahwa level gliserol berpengaruh nyata (p<0.05) terhadap motilitas dan recovery rate namun tidak berpengaruh nyata terhadap abnormalitas sperma. Perlakuan level gliserol 5% nyata (p<0.05) menghasilkan motilitas (40,47%) dan recovery rate (46,53%) tertinggi dibanding perlakuan lainnya. Disimpulkan bahwa semen Domba Lokal memiliki kualitas yang baik dan memenuhi syarat untuk dilakukan pengolahan lebih lanjut sampai semen beku dengan level gliserol 5% menghasilkan kualitas semen post-thawing yang paling optimal.
Pengaruh kombinasi pH media dan lama inkubasi terhadap ukuran panjang, lebar, dan luas kepala sperma hasil sexing Ibrahim, M. Rangga Rangga Katar; Solihati, Nurcholidah; Setiawan, Rangga
Jurnal Produksi Ternak Terapan Vol 6, No 3 (2025): Volume 6 Nomor 3 November 2025
Publisher : Faculty of Animal Husbandry, Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/jptt.v6i3.65924

Abstract

Sexing sperma metode swim up merupakan proses pemisahan spermatozoa X danY yang paling mudah dan ekonomis. Penelitian ini bertujuan untuk mengetahui pengaruh kombinasi pH media dan lama inkubasi terhadap morfometri sperma pembawa kromosom X-Y pada kambing sapera hasil sexing. Penelitian ini menggunakan Rancangan Acak Lengkap dengan enam perlakuan kombinasi pH dan lama inkubasi, yaitu P1 (6,2-40), P2 (6,8-40), P3 (7,4-40), P4 (6,2-60), P5 (6,8-60), P6 (7,4-60), setiap perlakuan diulang 5 kali. Data penelitian dianalisis menggunakan analisis varian dengan software statplus. Hasil penelitian menunjukkan rataan minimal dan maksimal ukuran panjang 7,49 ± 0,05 dan 10,03 ± 0,11 µm, serta lebar 3,54 ± 0,07 dan 5,37 ± 0,05 µm. Hasil Penelitian menunjukkan bahwa kombinasi pH media dan lama inkubasi tidak berpengaruh nyata terhadap morfometri X-Y pada lapisan atas dan bawah (Fhit ≤ F0,05). Disimpulkan bahwa perlakuan memberikan pengaruh yang sama.
Co-Authors ALI, SYAHRIR FAJRUL Anggraeni, Fitri Dian Annisaa Yusrina Annisaa Yusrina Anwar Anwar Anwar Anwar Citaliani Rostika, Yulia Eros Sukmawati F.A. Pamungkas HAFILAH, IDZNI Hamdi, Tasya Puteri Aulia Heni Indrijani I. Y. Asmara Ibrahim, M. Rangga Rangga Katar j J. Arifin Kikin Winangun Maulana, Gifari Ahmad Nena Hilmia Nena Hilmia Nena Hilmia Nuraeni, Ina NURHAYATI, SITI ZYA Nurul Ikhwan Petrus Kune Rangga Setiawan Rangga Setiawan Rasad, Siti Darojah Rini Widyastuti Romina, Saskia Putri Ruhijat Idi Salsa Adila Irianti Santi Nurjanah, Santi Septiyani Septiyani, Septiyani Siti Darodjah Rasad Siti Darodjah Rasad Soeparna Soeparna, Soeparna Sukirman, Iman t T. Hariyanti Tita D. Lestari Toha . Toha, Toha Tuti Kustini Winangun, Kikin