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All Journal CAKRA KIMIA (Indonesian E-Journal of Applied Chemistry) E-Jurnal Manajemen Universitas Udayana Bali Medika Jurnal Abdimas Galuh: Jurnal Pengabdian Kepada Masyarakat Jurnal Pengabdian kepada Masyarakat Wahana Usada Jurnal Kesehatan Rajawali JRKN Journal of Innovation Research and Knowledge Jurnal Abdi Kesehatan dan Kedokteran Seminar Ilmiah Nasional Teknologi, Sains, dan Sosial Humaniora (SINTESA) Seminar Nasional Aplikasi Iptek (SINAPTEK) SERVIRISMA: Jurnal Pengabdian kepada Masyarakat Health & Medical Sciences Obat: Jurnal Riset Ilmu Farmasi dan Kesehatan Jurnal Pengabdian Kepada Masyarakat Bersinergi Inovatif Multidisciplinary Indonesian Center Journal Jurnal MediLab Mandala Waluya
Nyoman Sudarma
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Religion Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Dentistry Earth & Planetary Sciences Economics, Econometrics & Finance Education Electrical & Electronics Engineering Health Professions Immunology & microbiology Law, Crime, Criminology & Criminal Justice Medicine & Pharmacology Nursing Public Health Social Sciences Veterinary Other

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PEMANFAATAN BETADINE SEBAGAI INDIKATOR UJI KLORIN PADA BERAS BERPEMUTIH: UTILIZATION OF BETADINE AS A CHLORINE TEST INDICATOR ON WHITE RICE Sudarma, Nyoman; Idayani, Sri; Setiawan, Didik; Dharmawan, Putu Oka
Bali Medika Jurnal Vol 5 No 2 (2018): Bali Medika Jurnal Vol 5 No 2 Desember 2018
Publisher : Stikes Wira Medika Bali

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36376/bmj.v5i2.32

Abstract

Klorin merupakan salah satu penggunaan Bahan Makanan Tambahan yang dilarang. Menurut Peraturan Menteri Kesehatan Republik Indonesia No.033/Menkes/Per/IX/2012, bahwa klorin tidak tercatat sebagai Bahan Tambahan Pangan (BTP) dalam kelompok pemutihan dan pematang tepung. Klorin digunakan sebagai pemutih beras yang dimaksudkan agar beras memiliki kualitas super dengan harga yang tinggi. Masyarakat akan kesulitan membedakan beras yang mengandung klorin atau tidak sehingga perlu dilakukan uji sederhana yang dapat dilakukan oleh masyarakat luas. Povidon iodine atau dikenal dengan betadine yang merupakan bahan antiseptik luka merupakan salah satu alternatif digunakan untuk identifikasi secara kualitatif kandungan klorin baik pada makanan maupun air. Tujuan dari penelitian ini adalah untuk mengetahui apakah betadine dapat digunakan sebagai alternatif untuk identifikasi klorin pada sampel beras bermerk maupun non merk. Hasil penelitian menunjukkan bahwa betadine dapat digunakan sebagai indikator identifikasi klorin pada sampel beras yang dijual di pasaran. Sepuluh sampel beras yang diidentifikasi dua diantaranya terindikasi positif mengandung pemutih klorin. Sampel beras positif mengandung klorin jika setelah penambahan dengan betadine menghasilkan warna putih keruh. Uji penegasan dilakukan dengan menambahkan larutan amilum dan KI 10% pada sampel beras dan menghasilkan warna biru kehitaman. Kata kunci : beras, pemutih, klor, betadine ABSTRACT Chlorine is one of the prohibited uses of Foodstuffs. According to the Regulation of the Minister of Health of the Republic of Indonesia No.033 / Menkes / Per / IX / 2012, that chlorine is not recorded as a Food Additives (BTP) in the bleaching and flour milling group. Chlorine is used as rice bleach which is intended to have a super quality rice at a high price. The community will find it difficult to distinguish whether or not rice contains chlorine or not, so a simple test can be carried out by the community. Povidon iodine, also known as betadine, which is an antiseptic wound, is one alternative used to qualitative identify chlorine content in food and water. The purpose of this study was to determine whether betadine can be used as an alternative for identification of chlorine in samples of branded and non-branded rice. The results showed that betadine could be used as an indicator of chlorine identification in rice samples sold in the market. Ten rice samples were identified, two of which were indicated to be positive for chlorine bleach. The rice sample is positive for chlorine if after adding it with betadine it produces a cloudy white color. The affirmation test was carried out by adding a solution of starch and 10% KI to the rice sample and producing a blackish blue color. Key words: rice, bleach, chlorine, betadine
Analisis kadar paracetamol pada darah dan serum SIS KADAR PARACETAMOL PADA DARAH DAN SERUM: Analysis of paracetamol levels in blood and serum Sudarma, Nyoman; Subhaktiyasa, I Putu Gede
Bali Medika Jurnal Vol 8 No 3 (2021): Special Issue Bali Medika Jurnal Vol 8 No 3 Oktober 2021
Publisher : Stikes Wira Medika Bali

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36376/bmj.v8i3.177

Abstract

Paracetamol is a group of analgesics that is pain relief in the way of work and inhibits the synthesis of prostaglandins especially in the central nervous system. Paracetamol given orally will be absorbed quickly and reach peak serum levels within 30-120 minutes. The purpose of this research is to determine the level of paracetamol in blood and serum. The sample used were blood and serum samples from respondents who consumed the 500 mg paracetamol drug. Blood samples were taken 2 hours after consuming the drug paracetamol. To get serum, blood must be centrifuged, with the process of centrifugation of confounding substances in the blood can be minimized. Qualitative analysis of paracetamol levels in blood and serum was analyzed using gas chromatography-mass spectrometry (GC-MS). Paracetamol extraction on blood and serum samples using the Solid Phase Extraction (SPE) method. The extraction results were derivatized using BSTFA containing 1% TMCS and analyzed using gas chromatography-mass spectrometry (GC-MS). Quantitative analysis was performed by calculating paracetamol levels using the equation of the regression line y = 50207x + 56321. The results of this research showed that blood and serum samples showed positive paracetamol at 15,056 and 15,101 retention times. The level of paracetamol in blood samples was 175,2 ppm and the level of paracetamol in serum samples was 56,7 ppm.
EDUKASI METODE MINDFUL JOURNALING DALAM PENINGKATAN STRATEGI MANAJEMEN STRES PADA REMAJA Sanjiwani, Anak Agung Sri; Wati, Ni Made Nopita; Dewi, Ni Luh Putu Thrisna; Lisnawati, Ketut; Sudarma, Nyoman
Servirisma Vol. 5 No. 1 (2025): Servirisma : Jurnal Pengabdian kepada Masyarakat
Publisher : Lembaga Penelitian dan Pengabdian Masyarakat (LPPM) Universitas Kristen Duta Wacana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21460/servirisma.2025.51.93

Abstract

Stress is one of the mental health problems that can be experienced by adolescents. The dynamics of the transition to adulthood can create various challenges for adolescents. This is also accompanied by high peer competition, environmental demands, the urge to show themselves and other factors making adolescents a vulnerable group related to mental health issues. Stress, even in the mild category, if unrecognized and untreated can lead to greater potential for mental health problems. Based on this, the community service activities carried out aim to provide education to adolescent students regarding strategies in managing daily stress and introduce one of the strategies that can be done for stress management by doing mindful journaling. The methods provided were lectures, discussions and writing simulations for 24 students at the vocational high school level. The results of the community service activities showed that there was an increase in knowledge after being given the material as well as the ability to identify stressors, thoughts-feelings and evaluate actions in dealing with the stressors faced through mindful journaling exercises.
IDENTIFIKASI SIANIDA PADA SPESIMEN PLASMA BERDASARKAN LAMA PENYIMPANAN SUHU RUANG Ni Kadek Arik Puspayanti; Nyoman Sudarma; Ni Luh Nova Dilisca Dwi Putri
Journal of Innovation Research and Knowledge Vol. 5 No. 2: Juli 2025
Publisher : Bajang Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Sianida dalam tubuh dapat menghambat fungsi pengangkutan oksigen dengan membentuk cyanohemoglobin. Sifatnya yang sangat beracun dan mudah masuk ke tubuh melalui inhalasi, konsumsi, atau kontak kulit, pemeriksaan kadar sianida (CN⁻) dan SCN⁻) dalam darah khususnya plasma menjadi sangat penting. Penanganan plasma adalah langkah kritikal dalam memastikan akurasi hasil pemeriksaan laboratorium. Penelitian ini bertujuan untuk mengetahui pengaruh lama penyimpanan spesimen plasma darah pada suhu ruang (25°C) terhadap kadar sianida. Metode yang digunakan adalah eksperimen kuantitatif pendekatan analitik. Sampel plasma yang mengandung standar sianida, disimpan dengan variasi waktu 0, 1, 2, 4, 8, dan 24 jam dan diukur kadar sianida dengan test kit untuk tiap waktu penyimpanan. Hasil penelitian menunjukkan selama waktu penyimpanan terjadi penurunan kadar sianida dalam plasma yaitu pada 0 jam kadar sianida sebesar 0,0400 ppm menurun sampai penyimpanan 24 jam menjadi 0,0167 ppm. Uji Kruskal-Wallis diperoleh nilai signifikansi sebesar 0,01 (p<0,05) yang menunjukkan adanya perbedaan kadar sianida plasma yang signifikan antar waktu 0 jam hingga 24 jam. Uji Mann-Whitney U menunjukkan bahwa penurunan siginifikan terjadi pada waktu penyimpanan 2 jam. Menurunnya kadar sianida plasma dierkuat dengan uji korelasi Spearman menunjukkan hubungan negatif antara waktu penyimpanan dan kadar sianida (r = -0,918). Semakin lama plasma disimpan pada suhu ruang, semakin rendah kadar sianida yang terdeteksi. Penurunan kadar sianida disebabkan oleh degradasi kimia selama penyimpanan
RELATIONSHIP OF BODY MASS INDEX WITH HbA1c MEASURES IN PATIENTS AT PRIMA MEDIKA DENPASAR HOSPITAL S.A.N Diah Ratnasari Warassaty; Prasetya, Didik; Nyoman Sudarma
Multidisciplinary Indonesian Center Journal (MICJO) Vol. 2 No. 3 (2025): Vol. 2 No. 3 Edisi Juli 2025
Publisher : PT. Jurnal Center Indonesia Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62567/micjo.v2i3.920

Abstract

The rising prevalence of obesity in Indonesia contributes to increased risk of metabolic diseases such as diabetes mellitus. Body Mass Index (BMI) is commonly used to assess nutritional status and may be correlated with long-term blood glucose levels measured by Hemoglobin A1c (HbA1c). This study aimed to explore the relationship between BMI and HbA1c levels in patients at Prima Medika General Hospital, Denpasar. A cross-sectional analytic observational design was employed, involving 30 purposively selected respondents. BMI was calculated from weight and height measurements, while HbA1c levels were assessed using the immunoturbidimetric method. Results showed an even distribution of respondents in the normal and overweight BMI categories (each 46.5%), while 50% had HbA1c levels >8%. However, Pearson correlation analysis indicated no statistically significant relationship between BMI and HbA1c levels (p=0.982; r=-0.004). The study concludes that BMI does not have a linear correlation with HbA1c levels. Other factors such as type and duration of therapy, disease progression, and patient adherence may play a greater role in influencing glycemic control and should be further investigated.
DESCRIPTION OF LOW DENSITY LIPOPROTEIN (LDL) LEVELS AND FASTING COMPLIANCE IN OUTPATIENT CARDIAC PATIENTS AT RSU PRIMA MEDIKA DENPASAR IN THE MONTHS OF FEBRUARY TO MARCH 2025 Ni Putu Martiana Prawita Sari; Nyoman Sudarma; Prasetya, Didik
Multidisciplinary Indonesian Center Journal (MICJO) Vol. 2 No. 3 (2025): Vol. 2 No. 3 Edisi Juli 2025
Publisher : PT. Jurnal Center Indonesia Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62567/micjo.v2i3.938

Abstract

Cardiovascular disease remains a leading cause of death both globally and nationally, with Low Density Lipoprotein(LDL) levels serving as a key biochemical indicator in the screening and management of heart disease. Accurate LDL testing requires adherence to pre-analytical procedures, particularly fasting for 10-12 hours. This study aimed to describe LDL levels and fasting compliance among outpatient cardiac patients at RSU Prima Medika Denpasar. A descriptive cross-sectional design was used with purposive sampling, involving 35 cardiac outpatients from February to March 2025. Data were collected through interviews and laboratory examinations using the Furuno CA-270 device. Results showed that 71% of respondents had LDL levels >100 mg/dL, indicating high levels, while 29% hadnormal levels (<100 mg/dL). All respondents (100%) complied with the fasting requirement. These findings indicate that high LDL levels are still commonly found among outpatient cardiac patients, particularly in productive-age males. The high level of fasting compliance reflects the effectiveness of pre-analytical patient education. This study emphasizes the importance of routine lipid screening and continuous education to enhance laboratory diagnostic accuracy and prevent cardiovascular complications.
Hubungan Pengetahuan Ahli Teknologi Laboratorium Medis Terhadap Perilaku Penggunaan Alat Pelindung Diri Di Rumah Sakit Kota Sangatta Santi, Rasmiati; Ni Luh Gede Puspita Yanti; Nyoman Sudarma
Health & Medical Sciences Vol. 2 No. 4 (2025): August
Publisher : Indonesian Journal Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47134/phms.v2i4.457

Abstract

Laboratorium merupakan tempat kerja dengan tingkat resiko bahaya tinggi karena melibatkan bahan-bahan kimia yang berbahaya. Tujuan penelitian untuk mengetahui hubungan tingkat pengetahuan ahli teknologi laboratorium medis terhadap perilaku penggunaan APD di Rumah Sakit Kota Sangatta. Metode penelitian ini adalah deskriptif korelasional dengan melibatkan seluruh Ahli Tenaga Laboratorium rumah sakit se-kota Sangatta sebagai responden. Instrumen penelitian menggunakan kuisioner yang berisi pernyataan terkait pengetahuan dan perilaku penggunaan APD. Data hasil penelitian diperoleh sebagian besar dengan pengetahuan tinggi yaitu 48 (85,8%) responden dan perilaku penggunaan APD baik sebanyak 29 (52,2%) responden. Dengan hasil yang di dapat pada uji Chi Square 0,465; α=0,05 menunjukan pengetahuan Ahli Teknologi Laboratorium Medik terhadap APD tidak ada hubungan yang signifikan antara tingkat pengetahuan dengan perilaku penggunaan APD. Hasil uji statistik menyatakan bahwa tidak ada hubungan diantara keduanya. Tingginya tingkat pengetahuan Ahli Teknologi Laboratorium Medis tentang APD ternyata tidak sejalan dengan peningkatan perilaku penggunaan APD dengan baik
PENGARUH WAKTU PENYIMPANAN SAMPEL DARAH EDTA PADA SUHU RUANGAN TERHADAP NILAI TROMBOSIT DAN LEUKOSIT Nur Alam; I Gusti Putu Agus Ferry Sutrisna Putra; Nyoman Sudarma
Jurnal MediLab Mandala Waluya Vol. 9 No. 1 (2025): JURNAL MEDILAB MANDALA WALUYA
Publisher : Prodi D4 Teknologi Laboratorium Medis, Universitas Mandala Waluya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.54883/medilab.v9i1.1224

Abstract

Penelitian ini bertujuan untuk mengetahui pengaruh lama penyimpanan sampel darah EDTA pada suhu ruang terhadap perubahan nilai leukosit dan trombosit. Sampel darah sebanyak 40 orang diperiksa menggunakan desain eksperimen pretest-posttest pada tiga waktu pemeriksaan, yaitu pemeriksaan segera setelah pengambilan darah, setelah penundaan penyimpanan selama 3 jam, dan setelah penundaan selama 6 jam pada suhu ruang. Karena sebagian besar data tidak memenuhi asumsi normalitas, maka analisis statistik menggunakan uji non-parametrik Kruskal-Wallis untuk menguji perbedaan nilai leukosit dan trombosit antar kelompok waktu penyimpanan. Hasil analisis menunjukkan adanya perbedaan yang signifikan secara statistik pada nilai leukosit (p = 0,010) dan trombosit (p = 0,023) di antara ketiga kelompok waktu pemeriksaan. Hal ini mengindikasikan bahwa lama penyimpanan sampel darah pada suhu ruang berpengaruh terhadap perubahan jumlah leukosit dan trombosit yang terdeteksi. Perubahan tersebut kemungkinan disebabkan oleh proses degradasi biologis dan kerusakan sel akibat paparan suhu ruang yang berkepanjangan. Penelitian ini memberikan gambaran penting mengenai batas waktu optimal penyimpanan sampel darah EDTA sebelum pemeriksaan hematologi dilakukan, sehingga dapat membantu laboratorium dalam menjaga akurasi dan reliabilitas hasil pemeriksaan darah. Dengan demikian, rekomendasi waktu penyimpanan yang tepat sangat diperlukan untuk meminimalisir kesalahan interpretasi hasil pemeriksaan klinis.
Edukasi Melalui Flipbook Berbasis Digital Sebagai Upaya Pencegahan Resiko Penyalahgunaan Narkoba Pada Remaja: Digital Flipbook-Based Education as an Effort to Prevent the Risk of Drug Abuse Among Adolescents Wati, Ni Made Nopita; Thrisna Dewi, Ni Luh Putu; Lisnawati, Ketut; Sanjiwani, A.A Sri Sanjiwani; Sudarma, Nyoman
Jurnal Abdi Keperawatan dan Kedokteran Vol 4 No 2 (2025): Jurnal Abdi Kesehatan dan Kedokteran
Publisher : Chakra Brahmanda Lentera Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55018/jakk.v4i2.86

Abstract

Drug abuse among adolescents continues to rise and poses a serious threat to the future of the younger generation. Existing educational efforts still rely heavily on conventional methods that are less appealing to today’s digital-native youth. Thus, an innovative and interactive educational medium is urgently needed. This community service project applied a participatory-educational approach and was conducted at a vocational health school in Bali. Participants included school counselors and students. Activities included situation analysis, development and validation of a digital flipbook, delivery of drug education using the flipbook, and evaluation of knowledge through pre- and post-tests. The use of digital flipbook significantly improved participants' knowledge. The proportion of students with good knowledge increased from 7.4% to 81.5%, while those with poor knowledge drastically decreased. Participants found the flipbook engaging and easy to use. The digital flipbook is an effective educational tool to enhance adolescent knowledge on drug abuse prevention. It is recommended for integration into school-based youth development and anti-drug programs.
EFFECT OF DELAY TIME IN URINE EXAMINATION ON ALCOHOL CONCENTRATION Dabi, Aberina; Prihatiningsih, Diah; Sudarma, Nyoman
Multidisciplinary Indonesian Center Journal (MICJO) Vol. 2 No. 4 (2025): Vol. 2 No. 4 Edisi Oktober 2025
Publisher : PT. Jurnal Center Indonesia Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62567/micjo.v2i4.1283

Abstract

Urine alcohol testing plays a vital role in detecting alcohol consumption for clinical and forensic purposes, yet its results may be influenced by pre-analytical factors such as the delay between sample collection and laboratory analysis. This study aimed to evaluate the effect of delay time before urine examination on alcohol concentration. An experimental One-Shot Case Study design was conducted involving 44 participants in Denpasar who had consumed alcohol. Alcohol concentration was measured immediately, after a 2-hour delay, and after a 4-hour delay using the Alcohol Urine Rapid Test Dipstick Allchek, and the data were analyzed using the Kruskal-Wallis test. The findings showed that average urine alcohol levels increased numerically after a 2- and 4-hour delay compared to immediate testing; however, this change was not statistically significant (p=0.131). These results confirm that delaying urine alcohol examination up to 4 hours does not significantly alter detectable alcohol concentrations, although prompt testing is still recommended to ensure result accuracy and minimize the risk of false positives. This study provides important insights for the development of standard operating procedures regarding urine alcohol analysis in laboratory practice.
Co-Authors Abadi, Fairuz Dabi, Aberina Dharmawan, Putu Oka Diah Prihatiningsih Diani, Ni Luh Ayu Putri Gading Didik Setiawan Edy Novayana I Gusti Putu Agus Ferry Sutrisna Putra Irdhawati Irdhawati James Sibarani Lisnawati, Ketut Ni Kadek Arik Puspayanti Ni Luh Gede Puspita Yanti Ni Made Septi Ariani Ni Putu Martiana Prawita Sari Ni Wayan Desi Bintari Nur Alam Prasetya, Didik Putri, Ni Luh Nova Dilisca Dwi Putu Gede Subhaktiyasa Putu Gede Subhaktyasa Putu Thrisna Dewi, Ni Luh Retno Rusnaini S.A.N Diah Ratnasari Warassaty Sanjiwani, A.A Sri Sanjiwani, A.A Sri Sanjiwani Sanjiwani, Anak Agung Sri Santi, Rasmiati Sri Idayani, Sri Susi, Ni Nengah Thrisna Dewi, Ni Luh Putu Timorensa, Katharina Novika Wati, Ni Made Nopita