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All Journal CAKRA KIMIA (Indonesian E-Journal of Applied Chemistry) Klinikal Sains (Jurnal Analis Kesehatan) Bali Medika Jurnal Abdimas Galuh: Jurnal Pengabdian Kepada Masyarakat Jurnal Pengabdian kepada Masyarakat Wahana Usada Jurnal Kesehatan Rajawali JRKN Journal of Innovation Research and Knowledge Jurnal Abdi Kesehatan dan Kedokteran Seminar Ilmiah Nasional Teknologi, Sains, dan Sosial Humaniora (SINTESA) Seminar Nasional Aplikasi Iptek (SINAPTEK) Health & Medical Sciences Obat: Jurnal Riset Ilmu Farmasi dan Kesehatan Jurnal Pengabdian Kepada Masyarakat Bersinergi Inovatif Multidisciplinary Indonesian Center Journal Jurnal MediLab Mandala Waluya
Nyoman Sudarma
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Gambaran Pemeriksaan Telur Cacing Soil Transmitted Helminths pada Pekerja Sampah di TPA Suwung dengan Pewarnaan Kunyit Kering dan Eosin Metode Direct Slide Diani, Ni Luh Ayu Putri Gading; Idayani, Sri; Sudarma, Nyoman
Jurnal Kesehatan Rajawali Vol 15 No 2 (2025): Jurnal Kesehatan Rajawali
Publisher : Sekolah Tinggi Ilmu Kesehatan Rajawali

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.54350/jkr.v15i2.574

Abstract

Soil-Transmitted Helminths (STH) infections are a significant public health issue in tropical regions, particularly among communities with poor sanitation, such as waste workers at the Suwung Landfill in Denpasar. Helminth egg examination is commonly conducted using the direct smear method with eosin staining; however, synthetic dyes have limitations such as high cost and environmental concerns. This study aimed to compare the staining results of STH eggs in fecal preparations using natural dried turmeric dye and eosin through the slide direct method. This research is descriptive in nature, using a qualitative approach. involving 30 STH-positive fecal samples stained using a dried turmeric extract solution (5 g turmeric powder in 20 mL of 96% ethanol) and eosin. Observation parameters included egg color intensity and background contrast. The results showed that staining with dried turmeric revealed 2 positive samples (13.33%), while eosin staining revealed 5 positive samples (33.3%) for STH eggs. The identified helminth species was Ascaris lumbricoides. Eosin was found to be more effective in highlighting the morphology of helminth eggs. However, dried turmeric still shows potential as a supplementary stain due to its accessibility, affordability, and environmental friendliness.
Perbedaan Hasil Pemeriksaan Sianida Urine dengan Variasi Waktu Penundaan Kadar Awal (1, 2, 4, 8, 24 Jam) yang Disimpan Pada Suhu Ruang dan Rentang Suhu 2-8°C Susi, Ni Nengah; Sudarma, Nyoman; Abadi, Fairuz
Jurnal Kesehatan Rajawali Vol 15 No 2 (2025): Jurnal Kesehatan Rajawali
Publisher : Sekolah Tinggi Ilmu Kesehatan Rajawali

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.54350/jkr.v15i2.576

Abstract

This study aims to determine the differences in the results of cyanide levels in urine stored at room temperature (25°C) and cold temperatures (2–8°C) with variations in the initial delay time (1, 2, 4, 8, and 24 hours). Urine samples that have been added with standard KCN solution were analyzed using a colorimetric test kit. Data were tested using a two-way ANOVA method using Aligned Rank Transformation (ART) followed by post-hoc tests (emmeans and contrast). The results showed that temperature and time factors had a significant effect individually on cyanide levels (p <0.001), but there was no significant interaction between the two (p <0.017511) this value is greater than 0.01 so it can be concluded that there is no significant effect of the interaction between temperature and time on cyanide levels. The highest cyanide levels were recorded at the initial level of 0.0400 ppm and decreased significantly over time to 0.0167 ppm at room temperature. and at cold temperatures it is more capable of maintaining cyanide levels to 0.0267 ppm. Storage at cold temperatures is more capable of maintaining cyanide levels compared to room temperature. These results indicate the importance of temperature management and storage time in maintaining the validity of cyanide examination results in urine.
PENGARUH PENUNDAAN PEMERIKSAAN SPESIMEN URINE DENGAN PENYIMPANAN PADA COOLBOX TERHADAP VIABILITAS BAKTERI Ni Wayan Desi Bintari; Nyoman Sudarma
Klinikal Sains : Jurnal Analis Kesehatan Vol. 12 No. 2 (2024): Desember
Publisher : LPPM Universitas Abdurrab

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36341/klinikal_sains.v12i2.5160

Abstract

The natural course of the disease in patients with urinary tract infections (UTIs) is often unpredictable, so a proper diagnosis is needed through accurate laboratory tests. Urine specimen management is important for laboratory staff to pay attention to because delays in examination risk increasing false positive or negative results. The purpose of this study was to determine whether there was an effect of delaying the examination of urine specimens stored in a cool box on the viability of bacteria from urine culture examination results. The type of research was experimental, conducted at the Bacteriology Laboratory of STIKES Wira Medika Bali in June-August 2024. The experimental group consisted of delays in urine specimen examination for 0 hours, 2 hours, 4 hours and 6 hours. The calculation of bacterial viability was carried out by examining urine cultures with a calibrated loop technique. The research data were analyzed using the Kruskal Wallis bivariate test. The results showed that there was no significant difference in the viability of bacteria from urine culture at delays of 0 hours, 2 hours, 4 hours and 6 hours (p> 0.05) stored in a cool box. Urine specimens for calculating bacterial viability were still stable in cool box storage up to a delay time of 6 hours. It is suggested that further research is needed to determine whether there is a difference in bacterial viability in specimens stored in a cool box with a delay time of > 6 hours.
Co-Authors Abadi, Fairuz Dabi, Aberina Dharmawan, Putu Oka Diah Prihatiningsih Diani, Ni Luh Ayu Putri Gading Didik Setiawan Edy Novayana I Gusti Putu Agus Ferry Sutrisna Putra Irdhawati Irdhawati James Sibarani Lisnawati, Ketut Ni Kadek Arik Puspayanti Ni Luh Gede Puspita Yanti Ni Made Septi Ariani Ni Wayan Desi Bintari Nur Alam Prasetya, Didik Putri, Ni Luh Nova Dilisca Dwi Putu Gede Subhaktiyasa Putu Gede Subhaktyasa Putu Thrisna Dewi, Ni Luh Retno Rusnaini Sanjiwani, A.A Sri Sanjiwani, A.A Sri Sanjiwani Sanjiwani, Anak Agung Sri Santi, Rasmiati Sri Idayani, Sri Susi, Ni Nengah Thrisna Dewi, Ni Luh Putu Timorensa, Katharina Novika Wati, Ni Made Nopita