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Development of SCAR Marker for Detection of Sex Expression in Papaya (Carica papaya L.) from Several Genetic Backgrounds Sobir, ,; Sujiprihati, Sriani; Pandia, Evalina C.
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 36 No. 3 (2008): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Papaya plants are hermaphrodite, pistillate, or staminate. Sex inheritance in papaya is determined by a single gene locus with three alleles of M which is dominant for maleness, MH for hermaphrodites and m which is recessive for femaleness. Only fruits from hermaphrodite plants are marketed since they have the necessary commercial characteristics, i.e., they are pear-shaped and have thicker flesh and a smaller internal cavity. Increased papaya yield has been limited mainly by the ratio of female to hermaphrodite (1: 2) plants normally occurring in orchards. This ratio causes great losses to papaya producers. Identification of seedlings sex during nursery stage is of prime iportance. In order to obtain simple DNA markers to identify sex expression in papaya, five SCAR markers of 20-21 primers were utilized. Examination of these markers into 24 genotypes of papaya from 12 populations of different genetic background revealed that pair of primer PKBT-5 had successfully differentiated male and hermaphrodite plants from female plants. Hence, PKBT-5 pair of primer can be utilized as DNA marker for sex expression character identification in papayas. Key words: Papaya, sex expression, SCAR marker

The Study of Genetic Diversity and Relationships on Carica sp. by Means of Random Amplified Polymorphic DNA (RAPD) Analysis Satori, Ahmad; Sujiprihati, Sriani; Sobir, ,
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 30 No. 1 (2002): Buletin Agronomi
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

The genus of Carica comprises 21 species, among them three species have been domesticated and cultivated. They are Carica papaya, Carica candamarcencis and Carica monoica. In Indonesia, two species have been cultivated viz. Carica papaya and Carica candamarcencis. In this study, four accessions of Carica papaya species and one accession of Carica candamarcencis species were analyzed using RAPD technique. We successfully amplified a total I of 40 fragments from these DNA genome by using 6 random primers with GC bases content:::: 60% of each primer. The number of fragments of each primer ranged from 5 to 8 averaged 6.7 fragments per primer. Out of total fragments, 90% showed as polymorphic ranged from 5 to 8 on average 6 fragments per primer. A dendogram based on the UPGMA- link method using Nei and Li similarity and Principal Component Analysis (PCA) plot separated the accessions into two main groups, the Carica papaya species on the one side and the Carica candamarcencis species on the other side. The result demonstrated that RAPD analysis was able to reveal genetic difference between Carica papaya and Carica candamarcencis species, as well as genetic diversity in Carica papaya species.

Karakter Fisik dan Kimia Buah Pepaya pada Stadia Kematangan Berbeda Suketi, Ketty; Poerwanto, Roedhy; Sujiprihati, Sriani; Sobir, ,; Widodo, Winarso Drajad
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 38 No. 1 (2010): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

The objective of the experiment was to investigate the physical and chemical characteristics of three stadia of maturity based on a range of peel color from green to yellow or based on percentage of the yellow area of fruit peel (stadium 1 = 25-49 % yellow, stadium 2 = 50-74 % yellow, and stadium 3 = above 75 % yellow) on six genotypes of papaya. Each genotype exhibited different days to maturity for each stadium. The fruits of stadium 1, 2 and 3 for IPB 1 were picked at 130, 135, and 140 days after anthesis (DAA); IPB 10A at 160, 165, and 170 DAA; IPB 1 x PB 174 at 135, 140 and 145 DAA; while PB 174, IPB 1 x IPB 10A and IPB 10A x PB 174 were picked at 140, 145 and 150 DAA, respectively. The results indicated that peel firmness was affected by maturity stage on female fruit of IPB 10A. Maturity stage affected chemical characteristics of papaya included total soluble solids (TSS) content (IPB 10A, female fruit of PB 174, female fruit of IPB 1 x IPB 10A, and female fruit of IPB 1 x PB 174), vitamin C content (hermaphrodite fruit of 10 A, female fruit of IPB 1 x IPB 10A) and juice pH (hermaphrodite fruit of IPB 1). IPB 1 genotype can be harvested at all stadia of maturity stage. Hermaphrodite and female fruit of IPB 10 A, female fruit of PB 174, female fruit of IPB 1 x IPB 10A and female fruit of IPB 1 x PB 174 genotype would be better harvested at stadium 3 of maturity stage. Keywords: Carica papaya, papaya genotype, hermaphrodite fruit, female fruit, fruit quality, fruit maturity stage

Evaluasi Daya Hasil Cabai Hibrida dan Daya Adaptasinya di Empat Lokasi dalam Dua Tahun Syukur, Muhamad; Sujiprihati, Sriani; Yunianti, Rahmi; Kusumah, Darmawan Asta
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 38 No. 1 (2010): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

The aim of the experiment was to study the performance of yield components of eight pepper hybrids and their adaptation at four locations in two years (2008 and 2009). The experimental design used was Randomized Complete Block Design (RCBD), three replications as a block was nested in the location. Plant materials were eight hybrids (IPB CH1, IPB CH2, IPB CH3, IPB CH25, and four commercial hybrids were Adipati, Biola, Gada, and Hot Beauty). The IPB CH3 hybrid produced higher fruit width, fruit length, fruit weight, fruit flesh density and yield per plant compared to commercial hybrids. Days to flowering and days to harvesting of IPB CH3 was earlier than that of Hot Beauty. The performance of yield characters in Boyolali, Rembang and Subang was better than that in Bogor. The IPB CH3 hybrid was specifically adapted to Subang and Rembang, while IPB CH1, IPB CH2, IPB CH25 were suitable for Boyolali. Keywords: fruit, yield components, genotype x location interaction, Capsicum annuum L.

Heterosis dan Daya Gabung Karakter Agronomi Cabai (Capsicum annuum L.) Hasil Persilangan Half Diallel Daryanto, Ady; Sujiprihati, Sriani; Syukur, Muhamad
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 38 No. 2 (2010): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

The objective of this research was to study heterosis and heterobeltiosis effects of ﬁ fteen chili genotypes (Capsicum annuum L.), the general combining ability (GCA) and speciﬁ c combining ability (SCA) of six chili inbred lines through half diallel crosses. The experiment was conducted from November 2008 to June 2009 using a Randomized Complete Block Design with three replications at IPB experimental ﬁ eld, Leuwikopo, Darmaga. Heterosis values were predicted based on the average values of their parents whereas heterobeltiosis were predicted based on the average values of the highest parents. Analyses GCA and SCA were based on the Grifﬁ ng’s ﬁ xed model of diallel design method II. Genotype IPB C2 had the highest GCA for fruit weight and fruit length. Genotype IPB C15 had the highest GCA for yield per plant and percentage of marketable fruit. Hybrid IPB C2 x IPB C14 and IPB C9 x IPB C14 had the highest heterosis, heterobeltiosis, and SCA for all fruit characters and yield per plant. Keywords: chili, hybrid, heterobeltiosis, yield.

Kriteria Seleksi untuk Perakitan Varietas Cabai Tahan Phytophthora capsici Leonian Yunianti, Rahmi; Sastrosumarjo, Sarsidi; Sujiprihati, Sriani; Surahman, Memen; Hidayat, Sri Hendrastuti
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 38 No. 2 (2010): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Selection criteria for developing Phytophthora blight-resistant varieties of pepper had been done in both laboratory and ﬁ eld conditions. Resistance screening were conducted on 28-days-old pepper plants grown in 72-cell ﬂ ats by inoculating 5 mL of inoculum (contain 105 zoospore mL-1) to the base of each plant. Phytophthora capsici isolate used in this experiment was TG01, which then identiﬁ ed as race-3. The evaluation of pepper genotypes characteristics were conducted in ﬁ eld. Eight characters demonstrated a wide genetic variability; those characters were resistance to Phytophthora blight, infection period, dicotomous height, canopy width, fruit weight, fruit length, fruit diameter and production. These characters along with fruit wall thickness, chlorophyll-a, chlorophyll-b, total chlorophyll had high heritability values. Using path analysis infection period, dicotomous height and fruit diameter are recommended to be simultaneously used as selection criteria for developing Phytophthora blight resistant pepper varieties. Keywords : capsicum, Phytophthora capsici, selection criteria, path analysis

Analisis Kedekatan Hubungan antar Genotipe Pepaya Berdasarkan Karakter Morfologi dan Buah Suketi, Ketty; Poerwanto, Roedhy; Sujiprihati, Sriani; Sobir, ,; Widodo, Winarso D.
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 38 No. 2 (2010): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

A study was conducted to determine the variation and relationships among papaya genotypes based on morphological and fruit characteristics in order to produce high quality papaya fruits. Fruit characterization study is very useful for genotype improvement and genotype classiﬁ cation of papaya. In this study the morphological characters of 36 genotypes were analyzed to determine their phenotypic variabilities. The relationships between genotypes based on all of the morphological and fruit characteristics were tested by subjecting the data to multivariate principal component analysis and to cluster analysis. Based on the dendrogram generated from vegetative and generative characters, the 36 genotypes could be grouped into 11 clusters on a threshold of 1.6 and formed 6 clusters on a threshold of 1.8. The dendrogram was able to explain the close relationship between IPB 5 x IPB 1 and IPB 5 x IPB 4, IPB 2 and IPB 7, IPB 1 and IPB 3 genotype. The scattered diagram of generative variable divided the papaya genotypes into three groups based on fruit sizes i.e small group (IPB 1, IPB 3, IPB 4, IPB 3 x IPB 4, IPB 1 x IPB 9), medium group (IPB 5, IPB 7, IPB 8, IPB 9) and big group (IPB 2, IPB 10). The IPB 1, IPB 3 and IPB 4 were different from IPB 2 in fruit shapes, petal length of male ﬂ owers, inﬂ orescence size and fruit length. The hybrid plants obtained from crossings with IPB 10 were different from the other genotypes in the colours of female-, hermaphrodite-, and male ﬂ ower-lobes. Subsequently the scatter diagrams also revealed that several genotypes i.e. IPB 2 x IPB 6, IPB 1 x IPB 5, IPB 1 x IPB 9, IPB 5 x IPB 1 and IPB 5 x IPB 2 had superior characters ideotype similar to IPB 1, IPB 3 and IPB 8 genotypes. Keywords: Carica papaya, hermaphrodite, female, dendrogram, scatter diagram, ideotype

Selection method is one of the most important factors in determining the success of pepper breeding programs. Selection method will be effective if it is supported by a complete knowledge of genetic character inheritance. The aim of this research was to investigate the inheritance patterns to quantitative characters using large pepper (P1 (IPB C5)), curly pepper (P2 (IPB C105)), F1, F1R, BCP, BCP2 and F2. The result showed that dichotomous height, days to harvest and weight per fruit characters Arif, Abdullah Bin; Sujiprihati, Sriani; Syukur, Muhamad
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 40 No. 2 (2012): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24831/jai.v40i2.6359

Selection method is one of the most important factors in determining the success of pepper breeding programs. Selection method will be effective if it is supported by a complete knowledge of genetic character inheritance. The aim of this research was to investigate the inheritance patterns to quantitative characters using large pepper (P1 (IPB C5)), curly pepper (P2 (IPB C105)), F1, F1R, BCP, BCP2 and F2. The result showed that dichotomous height, days to harvest and weight per fruit characters were not maternally inherited. The additive-dominant genetic model was the most suitable for dichotomous height character. The additive-dominant with influence of interaction additive-dominant and interaction dominant-dominant genetic model was suitable for days to harvest character. The additive-dominant with influence of interaction additive-additive and interaction dominant-dominant genetic model was suitable for the weight of fruit character. Broad-sense and narrow-sense heritabilities range medium for dichotomous height and weight per fruit characters. Heritability of the days to harvest character was high in a broad-sense but was low in the narrow-sense. Keywords: genetic model, heritability, inheritance

Metode Pengusangan Cepat untuk Pengujian Vigor Daya Simpan Benih Cabai (Capsicum annuumL.) Ekowahyuni, Luluk Prihastuti; Sutjahjo, Surjono Hadi; Sujiprihati, Sriani; Suhartanto, Mohamad Rahmad; Syukur, Muhamad
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 40 No. 2 (2012): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

The ability of seed to maintain seed quality during storage is called seed vigor. Accurate method for seed vigor testing of pepper seeds is necessary to accurately determine seed storability during seed distribution. The aim of this research was to determine the best accelerated aging method (AAM) of pepper seed. Freshly harvested seeds of Capsicum annuumL., IPB C9 genotype were used in this study. The experiment used randomized complete block design (RCBD) with three replications. The experimental treatments were seed vigor testing methods i.e. natural deterioration test at room temperature in controlled humidity (RH 90-95%), and four AAM (hot water 60 ºC, methanol 20%, ethanol 20%, and high temperature at 40 ºC). The best method was selected using analysis of variance, coeficient of variance, regresion dan t student analysis. AAM using 20% methanol at periods of 0, 2, 4, 6 and 8 hours was the best method of seed vigor testing for pepper seed. Keywords: accelerated aging, deterioration, ethanol, methanol, high temperature

Non Paramectric Stability Analysis for Yield of Hybrid Chili Pepper (Capsicum annuum L.) Across Six Different Environments Syukur, Muhamad; Sujiprihati, Sriani; Yunianti, Rahmi; Kusumah, Darmawan Asta
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 42 No. 1 (2014): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

ABSTRACTThe objectives of this study were to evaluate several chili pepper hybrids whith high yielding character and stable at six environments based on some non-parametric stability analyses, and to study the correlation among the stability of non-parametric methods. The hybrid of chili pepper (7 candidates varieties and 5 commercial hybrid cultivars) were grown in a randomized complete block design with 3 replications in 6 different environments. Ten nonparametric stability methods were used to identify the stable genotypes. According to the SI(3), RS, NPi(1), NPi(2), NPi(3) and NPi(4) stability parameters, Imperial was the most stable hybrid. According to the SI(1), SI(2) and TOP stability parameters and yield, IPB CH3 was the most stable hybrid. In this study, the high TOP values were associated with the yield. Nonetheless, the results of the other non parametric (SI(6), NPi(3) and NPi(4)) were negatively correlated to the yield. The results also revealed that based on the non parametric stability test, the results could be classified into 2 groups, according to the agronomic and biological stabilities. Keywords: chili pepper, environment, non-parametric stability, yield

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