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Rachmad Saputra
Department Of Agrotechnology, Faculty Of Agriculture, Universitas Riau, Pekanbaru, Indonesia
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Penggunaan Bahan Organik dan Kombinasinya dalam Formulasi Biofungisida Berbahan Aktif Jamur Trichoderma pseudokoningii Rifai. untuk Menghambat Jamur Ganoderma boninense Pat. secara in vitro Elfina, Yetti; Ali, Muhammad; Saputra, Rachmad
Jurnal Natur Indonesia Vol 16, No 2 (2016)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (205.615 KB) | DOI: 10.31258/jnat.16.2.79-90

Abstract

Trichoderma pseudokoningii has been applied as a biocontrol agent against fungal plant pathogen, such as Ganoderma boninense, the cause of stem rot disease on palm oil plants. To be more effectively applicable in the field, some experiments have been employed to formulate T. pseudokoningii in a biofungicide formulation amended with organic matter as its main nutrient resource, zealot as a carrier agent and cocoyam powder as a mixture agent. A research has been conducted to study the effect of various organic matters and their combinations in a biofungicide formulation of T. pseudokoningii on growth inhibition to G. boninense in-vitro and to obtain the best organic matters and their combinations in enhancing the growth of T. pseudokoningii and yet inhibiting G. boninense. The research has been conducted in the Laboratory of Plant Pathology, Agriculture Faculty, University of Riau from May to August 2012, using a completely randomized design consisting of 15 treatments (bagasse, rice husk, shrimp shell, dregs, and their combinations) and 3 replications. The data were statistically analyzed using analysis of variance, followed by Duncan’s New Multiple Range Test (DNMRT). The results indicated that organic matters and their combinations in the biofungicide formulation significantly affected the antagonistic capacity of T. pseudokoningii in inhibiting the growth of G. boninense in vitro. Rice husk, bagases, bagasse+rice husks, and bagasse+dregs were the best organic matters in enhancing the growth of T. pseudokoningii and its capacity to inhibit G. boninense in-vitro. It can also be concluded that shrimp shell, bagasse+shrimp shell, rice husk+shrimp shell, shrimp shell+dregs and rice husk+shrimp shell+dregs totally inhibited the growth of T. pseudokoningii in the biofungicide formulation.
PEMBERDAYAAN KELOMPOK TANI DALAM PENGENDALIAN PENYAKIT TANAMAN DENGAN TEKNOLOGI BIOFUNGISIDA PLUS DI KABUPATEN KAMPAR Puspita, Fifi; Saputra, Rachmad; Adiwirman, Adiwirman; Ali, Muhammad; Idwar, Idwar; Armaini, Armaini; Nurbaiti, Nurbaiti; Yoseva, Sri; Zuhry, Elza
Jurnal Penelitian dan Pengabdian Kepada Masyarakat UNSIQ Vol 7 No 2 (2020): Mei
Publisher : Lembaga Penelitian, Penerbitan dan Pengabdian Masyarakat (LP3M) UNSIQ

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32699/ppkm.v7i2.963

Abstract

Kabupaten Kampar merupakan satu diantara beberapa sentra penanaman padi di Provinsi Riau. Kendala dalam melaksanakan budidaya tanaman padi yang masih ditemukan diantaranya adalah kurangnya pengetahuan petani tentang teknik budidaya dan pengendalian penyakit pada tanaman padi. Tujuan kegiatan ini ialah untuk mendorong petani agar dapat membuat biofungisida berbahan aktif konsorsium Trichoderma virens dan Pseudomonad berflourescens dalam formulasi Biofungisida Plus untuk pencegahan dan pengendalian penyakit yang menyerang tanaman padi. Kegiatan pemberdayaan petani di Desa Sawah melalui penerapan teknologi pengendalian penyakit tanaman padi dengan biofungisida plus dilaksanakan dengan menerapkan Model Community Development. Pada metode ini secara langsung dilibatkan sebagai subyek dan obyek dalam pelaksanaan kegiatan. Hasil yang diperoleh dari kegiatan pemberdayaan masyarakat ini diantaranya ialah, masyarakat bersedia untuk menerapkan teknologi Biofungisida Plus di lahan sawahnya. Berdasarkan hasil kuisioner juga diketahui pemahaman dan wawasan petani bertambah setelah diperkenalkan dengan Teknologi Biofungisida Plus ini. Aplikasi Biofungisida Plus di lahan petani menunjukkan kondisi tanaman yang lebih baik dibandingkan dengan teknik budidaya yang biasa digunakan oleh petani.
Kemampuan Penghambatan Formulasi Biofungisida Tepung Berbahan Aktif Trichoderma pseudokoningii Rifai Setelah Penyimpanan Terhadap Jamur Ganoderma boninense Pat. secara in vitro Rachmad Saputra; Yetti Elfina; Muhammad Ali
JURNAL BUDIDAYA PERTANIAN Vol 15 No 2 (2019): Jurnal Budidaya Pertanian
Publisher : Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/jbdp.2019.15.2.106

Abstract

Trichoderma pseudokoningii fungus has been tested as biological control agents against several pathogenic fungi, including the Ganoderma boninense fungus which causes basal stem rot disease on oil palm. However, in-field testing, the efficacy of antagonistic microbes still showed unstable results. Therefore, it is necessary to package antagonistic microbes in the form of formulations. This study aimed to determine the effect of storage time of flour biofungicide formulations on the viability and antagonism of T. pseudokoningii Rifai in inhibiting the G. boninense pathogenic fungus in vitro. The study was arranged in a Completely Randomized Design with 4 treatments: bagasse (F1), rice husks (F2), dregs (F4) and combinations of bagasse, rice husks and dregs (F12), with three replications. The results showed that the powder biofungicide formulation of F2 (rice husk) was able to maintain the viability of the T. pseudokoningii fungus as indicated by the high number of conidia and high growth rate compared to the other formulations. The T. pseudokoningii fungus in F2 formulation also showed the highest inhibition compared to other formulations after storage for 1 and 2 months. Keywords: biofungicide, formulation, Ganoderma boninense, Trichoderma pseudokoningii ABSTRAK Jamur Trichoderma pseudokoningii telah banyak diuji sebagai jamur antagonis pada beberapa jamur patogen termasuk jamur Ganoderma boninense yang menyebabkan penyakit busuk pangkal batang tanaman kelapa sawit. Namun, pada pengujian di lapangan, efikasi mikroba antagonis masih menunjukkan hasil yang tidak stabil. Oleh karenaya perlu pengemasan mikroba antagonis dalam bentuk formulasi. Penelitian ini bertujuan untuk mengetahui pengaruh waktu penyimpanan formulasi biofungisida tepung terhadap viabilitas dan daya antagonis T. Pseudokoningii Rifai dalam menghambat jamur G. boninense Pat. secara in vitro. Penelitian disusun dengan menggunakan rancangan acak lengkap dengan empat perlakuan: ampas tebu (F1), sekam padi (F2), dregs (F4) dan kombinasi ampas tebu, sekam padi dan dregs (F12), dengan tiga ulangan. Hasil penelitian menunjukkan bahwa formulasi biofungisida tepung F2 (sekam padi) mampu menjaga viabilitas jamur T. pseudokoningii yang ditunjukkan dengan banyaknya jumlah konidia dan tingginya kecepatan pertumbuhan dibandingkan dengan formulasi lainnya. Jamur T. pseudokoningii pada formulasi F2 juga menunjukkan daya hambat tertinggi dibandingkan dengan formulasi lainnya setelah penyimpanan 1 dan 2 bulan. Kata kunci: biofungisida, formulasi, Ganoderma boninense, Trichoderma pseudokoningii
Pemberdayaan petani karet rakyat dalam mengendalikan penyakit jamur akar putih dengan teknologi biofungisida tepung berbahan aktif Trichoderma virens endofit di Kecamatan Mempura Kabupaten Siak Fifi Puspita; Adiwirman Adiwirman; Elza Zuhry; Muhammad Ali; Erlida Ariani; Kausar Kausar; Rachmad Saputra
Unri Conference Series: Community Engagement Vol 1 (2019): Seminar Nasional Pemberdayaan Masyarakat
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/unricsce.1.210-218

Abstract

Community service activities in the form of activities of Desa Binaan through the application of biofungicide technology of Trichoderma virens endofit formulation in powder formulas was carried out at Lubuk Betuah Farmer Group, Pelimauan Jaya and Anugrah in Teluk Merempan Village Mempura District of Siak Regency. The purpose of this activity is to increasing knowledge of farmer groups in the formulation of Trichoderma virens endophytes in powder formulas and producing environmentally-friendly powder biofungicides so as to increase the production and quality of latex, in addition to ease the burden of farmers on fertilizer needs. From this activity is concluded several things, that are socialization of technology control program R. microporus in farmers group target 100% not knowing the use of T. virens as biofungisida. Training on the manufacture of flour biofungicide and the way biofungicide application for control of R. microporus 50% of the target farmer group has the desire to use T. virens biofungicide. Assistance of making flour biofungiside all the participants can make flour biofungicide. Next year activity is done monitoring and evaluation result of R. microporus control and mass production of flour biofungiside for increase the income of rubber farmers.
Pemberdayaan ibu PKK dalam pembuatan biopori berbasis limbah rumah tangga Isna Rahma Dini; Fauza Azmimelwa; Joko Setiawan; Rachmad Saputra
Unri Conference Series: Community Engagement Vol 2 (2020): Seminar Nasional Pemberdayaan Masyarakat
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/unricsce.2.24-30

Abstract

Various kinds of activities carried out by PKK mothers in filling their time working at home during the Covid-19 pandemic, one of which is farming around the yard. This farming activity certainly requires useful fertilizers to increase plant fertility. Biopores is an effort that can be made to improve soil health and trigger the natural decomposition of organic matter. The organic material used can come from kitchen waste. So far, biopores are known as an effort to overcome standing water and are still not widely used to increase soil fertility. This service aims to provide information on the manufacture and application of biopores in plants so that they can increase soil fertility in cultivated plants. The method used consists of lecturing methods, hands-on practice, and evaluating the achievement of activities using achievement measures. Based on the results of the posttest evaluation, there was an increase in the knowledge of PKK mothers about biopores, where initially only 40% increased to 100%. In addition, the direct practice of making and applying biopores carried out on one of the PKK mothers is an indicator of the high interest of PKK mothers in applying biopores. It can be concluded that community service activities are quite effective in increasing the ability and interest of PKK mothers in applying biopores to plants around their house yards.
Morphological characterization of Trichoderma spp. isolated from the oil palm rhizosphere in peat soils and its potential as a biological control for Ganoderma sp. in vitro Rachmad Saputra; Fifi Puspita; Anthony Hamzah; Irfandri; Eka Suryani
Jurnal Ilmiah Pertanian Vol. 19 No. 2 (2022): Jurnal Ilmiah Pertanian
Publisher : Universitas Lancang Kuning

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31849/jip.v19i2.9405

Abstract

Trichoderma sp. is a saprophytic fungus found in various environments, one of which is in the rhizosphere of oil palm plants which can be used to control Ganoderma and increase the resistance of oil palm plants from stem rot disease. This study aimed to characterize the morphology of Trichoderma spp. origin of peat land in oil palm plantations in Kampar Regency and screening the potential in controlling Ganoderma sp. Trichoderma spp. from oil palm rhizosphere was collected from the smallholder oil palm plantations in Deli Makmur Village, Kampar, Indonesia. This research was conducted by several observations, such as the hypovirulence test; identification of the morphology of Trichoderma spp. fungus; growth and diameter test of Trichoderma spp. fungus; test of the inhibitory ability of Trichoderma spp. fungus against Ganoderma sp. LPTUNRI-Gan002 isolate; test for hyperparasitism of the fungus Trichoderma spp., which has high antagonistic power against Ganoderma sp. isolate. Six Trichoderma spp. isolates had morphological characteristics similar to two species, i.e., Trichoderma harzianum (LPTUNRI-Trc001, Trc004, Trc005, and Trc006 isolates) and Trichoderma asperellum (LPTUNRI-Trc002 and Trc003). LPTUNRI-Trc003 had the highest diameter (90 mm), growth rate (32.66 mm/day), and the highest ability to suppress Ganoderma sp. LPTUNRI-Gan002 (91.03%) compared to the other five isolates.
Biological control agents potential and molecular identification of endophytic Trichoderma isolates originated from oil palm tissue against Ganoderma boninense Pat. Fifi Puspita; Titania T. Nugroho; Rachmad Saputra
Jurnal Ilmiah Pertanian Vol. 19 No. 2 (2022): Jurnal Ilmiah Pertanian
Publisher : Universitas Lancang Kuning

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31849/jip.v19i2.9402

Abstract

This research aimed to determine the potential of endophytic Trichoderma sp originated from oil palm plant tissue as a biological control agent and its molecular identification. The potency assay was carried out using a completely randomized design consisting of three isolates of Trichoderma sp. from different plant tissues (TR01 from root tissue, TS01 from stem tissue, and TM01 from midrib tissue) with inhibitory ability parameters. The inhibition of the growth of G. boninense was performed at the concentration of 15% and 20%, and the molecular identification of Trichoderma sp. endophyte isolates was analyzed using BLAST software. The results showed that Trichoderma sp. TM01 isolate had larger inhibitory than the other isolates on days 3 and 4 by 60% and 68%. TM01 isolate also showed inhibition to the growth of the G. boninense at 15% and 20%, with the percentage of inhibition reaching 71.79% and 82.05%. Based on phylogenetic analysis, the three endophytic isolates of Trichoderma sp. were closely related to Trichoderma virens.
PENGOLAHAN RIMPANG JAHE (Zingiber officinale Rosc.) SEBAGAI MINUMAN HERBAL PADA ERA NEW NORMAL DI DESA GUNUNG MULYA KABUPATEN KAMPAR Rachmad Saputra; Bela Safitri Wahyudi; Fajar Aulia Rahman; Muhammad Ammar Huseini; Harry Firmansyah; Arum Sekar Priyati; Hanifa Febriani; Nurul Fadhila Ramadhona; Putri Khairani; Rifa Shovia; Riska JH Jundiah
PAKDEMAS : Jurnal Pengabdian Kepada Masyarakat Vol 2 No 1 (2022): Desember
Publisher : Fakultas Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58222/pakdemas.v2i1.78

Abstract

Salah satu rempah termasyur saat ini adalah jahe karena bisa dimanfaatkan sebagai tanaman atau tumbuhan herbal. Jahe sendiri mengandung bahan atau zat aktif yang berguna untuk pengobatan. Jahe merah di Desa Gunung Mulya Kabupaten Kampar mudah untuk ditemukan karena banyak dibudidayakan. Indonesia baru saja mengambil kebijakan berupa membuka kembali aktivitas ekonomi, sosial dan kegiatan publik secara terbatas dengan menggunakan standar kesehatan atau biasa disebut dengan New Normal. Namun perlu diperhatikan penularan penyakit infeksi termasuk Covid-19 tidak terlepas dari interaksi antara agen penyebab penyakit dan lingkungan. Upaya peningkatan daya tahan tubuh perlu dilakukan agar dapat bertahan terhadap serangan antara lain dengan pemanfaatan bahan alam seperti jahe, dengan memanfaatkan jahe sebagai minuman herbal yang dibuat secara isntan. Metode yang digunakan untuk mencapai tujuan yang telah direncanakan adalah metode diskusi dan praktik. Dengan metode ini masyarakat diharapkan mampu memahami dan terampil dalam pembuatan minuman herbal dari jahe di Desa Gunung Mulya. Dengan demikian hal ini dapat meningkatkan imunitas masyarakat di Desa Gunung Mulya.
Assistance for Oyster Mushroom Entrepreneurs in Making Fish Feed Formulations for African Night Crawler Worm (ANC) and Trichoderma sp. Fermented Bran Dini, Isna Rahma; Saputra, Rachmad; Hapsoh, Hapsoh; Salbiah, Desita; Yoseva, Sri; Masjudi, Heri
Journal of Saintech Transfer Vol. 6 No. 2 (2023): Journal of Saintech Transfer
Publisher : Talenta Publisher Universitas Sumatera Utara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32734/jst.v6i2.10223

Abstract

Utilization of baglog waste as a growth medium for ANC worms as a raw material for making formulated fish feed provides opportunities as a side business for oyster mushroom entrepreneurs to increase income. The purpose of this service is to provide assistance to target partners, in this case oyster mushroom entrepreneurs, in utilizing ANC worm flour as a substitute for fish meal and then formulated with Trichoderma sp. fermented bran. The results of the dedication that has been carried out show that there has been an increase in partners' skills in making formulated fish fefazidah@usu.ac.ided and developing the potential of science and technology-based independent entrepreneurs who develop from mushroom businesses. After this dedication, it is hoped that the partners can continue to develop this worm farming business and the application of worm-formulated feed for fish farming which has also been developed by partners so as to create an integrated oyster mushroom-based farming center.
Streptomyces sp.: Characterization, Identification and Its Potential as a Ralstonia solanacearum Biological Control Agent in vitro Saputra, Rachmad; Arwiyanto, Triwidodo; Wibowo, Arif
Indonesian Journal of Agricultural Research Vol. 2 No. 3 (2019): InJAR, Vol. 2, No. 3, November 2019
Publisher : Talenta Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1041.563 KB) | DOI: 10.32734/injar.v2i3.1389

Abstract

Streptomyces sp. bacteria have the potential to produce antibiotic compounds, which are one of the mechanisms that are widely used in biological control. However, in general, biological control mechanisms also occur through competition, cell wall degradation and induced resistance. This study was aimed to determine the physiological, biochemical and molecular characteristics of two isolates of Streptomyces sp. (S-4 and S16 isolates) isolated from the tomatoes roots, and to find out their ability to control Ralstonia solanacearum, which causes bacterial wilt disease on a wide range of hosts. The results showed both Streptomyces sp. isolates had several different physiological and biochemical characteristics and had a different ability to inhibit R. solanacearum in vitro. Streptomyces sp. S-16 isolate had a high similarity with Streptomyces diastaticus subsp. ardesiacus strain NRRL B-1773T based on the molecular identification results. Further research needs to be done to see the potential inhibition of the two Streptomyces isolates in inhibiting the development of bacterial wilt disease in tomato plants caused by R. solanacearum.
Co-Authors Adiwirman Adiwirman Adiwirman Adiwirman, Adiwirman Adyatma Irawan Santosa Anthony Hamzah Anthony Hamzah Arif Wibowo Armaini, Armaini Arum Sekar Priyati Baehaqi Bela Safitri Wahyudi Besri Nasrul Desita Salbiah Eka Suryani Elza Zuhry Erlida Ariani Fajar Aulia Rahman Fauza Azmimelwa Fifi Puspita Fifi Puspita Fifi Puspita Haj Ali, Mayadah A. Hanifa Febriani Hapsoh Hapsoh Hapsoh Harry Firmansyah Idwar, Idwar Irfandri Irfandri Irfandri Irfandri Isna Dini Isna Rahma Dini Kausar Kausar Masjudi, Heri Muhammad Ali MUHAMMAD ALI Muhammad Ali Muhammad Ammar Huseini Novita Sari Depari Nurul Fadhila Ramadhona Nurwijayanti Pancasona, Muhammad Garda Pradhana, Adhi Putri Khairani Putro, Ananda Restu Rifa Shovia Riska JH Jundiah Shalihah, Filzah Sri Yoseva Sri Yoseva Surwadinata, Antama Titania Tjandrawati Nugroho Triwidodo Arwiyanto Yetti Elfina Yetti Elfina