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All Journal Jurnal Natur Indonesia Jurnal Perlindungan Tanaman Indonesia Jurnal Ilmiah Pertanian Indonesian Journal of Agricultural Research Jurnal Penelitian dan Pengabdian Kepada Masyarakat UNSIQ Journal of Saintech Transfer JURNAL BUDIDAYA PERTANIAN Unri Conference Series: Community Engagement PAKDEMAS : Jurnal Pengabdian Kepada Masyarakat Biodiversitas Journal of Biological Diversity
Rachmad Saputra
Department Of Agrotechnology, Faculty Of Agriculture, Universitas Riau, Pekanbaru, Indonesia
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Molecular Characterization of Mungbean yellow mosaic India virus and Tomato leaf curl New Delhi virus in Sukoharjo and Magelang Regencies, Indonesia Surwadinata, Antama; Putro, Ananda Restu; Pradhana, Adhi; Shalihah, Filzah; Pancasona, Muhammad Garda; Saputra, Rachmad; Haj Ali, Mayadah A.; Santosa, Adyatma Irawan
Jurnal Perlindungan Tanaman Indonesia Vol 29, No 1 (2025)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.101371

Abstract

Diseases caused by Begomoviruses in black-eyed pea (Vigna unguiculata subsp. unguiculata) and cucumber (Cucumis sativus) are remains insufficiently explored in Indonesia. One symptomatic black-eyed pea and two cucumber samples were collected from Sukoharjo and Magelang Regencies, respectively, to be molecularly tested using Krusty/Homer and SPG1/SPG2 primer pairs for universal detection of Begomoviruses. NCBI BLAST analysis on the obtained nucleotide sequences confirmed mungbean yellow mosaic India virus (MYMIV, Begomovirus vignaradiataindiaense) infection in black-eyed pea sample and tomato leaf curl New Delhi virus (ToLCNDV, Begomovirus solanumdelhiense) infection in both cucumber samples. Sequences of partial AV1, and partial AC1 and AC2 genes of the three isolates were registered with accession nos. PQ539469-71 and PQ539476-78, respectively, in NCBI GenBank. No recombination signal was detected in the sequences of the new isolates according to scan by Recombination Detection Program (RDP v.5.30). In the phylogenetic trees built by MEGA 11 software with Tamura-Nei parameter model, MYMIV H-2 as well as ToLCNDV N-8 and V-97 isolates shared basal nodes with Indonesian isolates, indicated their close genetic relationship with other isolates also found in the country. While expanding our information regarding genetic diversity of Begomoviruses, this study also reported the first cases of MYMIV in black-eyed pea in Indonesia and ToLCNDV in cucumber in Magelang, to the best of our knowledge.
Ganoderma diversity from smallholder oil palm plantations in peatlands of Kampar District, Indonesia based on mycelia morphology and somatic incompatibility Anthony Hamzah; Rachmad Saputra; Fifi Puspita; Besri Nasrul; Irfandri Irfandri; Novita Sari Depari
Biodiversitas Journal of Biological Diversity Vol. 22 No. 1 (2021)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d220103

Abstract

Abstract. Hamzah A, Saputra R, Puspita F, Nasrul B, Irfandri, Depari NS. 2021. Ganoderma diversity from smallholder oil palm plantations in peatlands of Kampar District, Indonesia, based on mycelia morphology and somatic incompatibility. Biodiversitas 22: 16-22. Basal Stem Rot disease is caused by the pathogenic fungus Ganoderma boninense which has caused major economic losses in the palm oil industry. Ganoderma boninense has been reported not only infecting crops in the field, but also attacking at the immature phase of the plant even in the nursery. Studies related to Ganoderma diversity in oil palm plantations in Riau, Indonesia have not been widely reported. Ganoderma genetic diversity is important because its provide information regarding the mechanism of infection and the spatial distribution. The Ganoderma spp. isolates were collected from three blocks of smallholder oil palm plantation in Deli Makmur Village, Kampar District, Riau Province, Indonesia and brought to Plant Disease Laboratory, Faculty of Agriculture, Riau University, Pekanbaru, Indonesia. This experimental research was conducted using six Ganoderma isolates (Gan1, Gan2, Gan3, Gan4, Gan5, and Gan6) on the parameters of colony diameter and growth speed, while for the parameters of the morphological diversity of Ganoderma mycelium and the diversity based on somatic incompatibility assay was carried out descriptively. The results of this study show that oil palm plants in smallholder plantations in Kampar District are infected by two groups of Ganoderma based on morphology characteristics using UPGMA dendrogram, but different among isolates based on genetically using somatic incompatibility assay. For the next confirmation, it is necessary to further identify whether the six isolates are different species using molecular identification.
Co-Authors Adiwirman Adiwirman Adiwirman Adiwirman, Adiwirman Adyatma Irawan Santosa Anthony Hamzah Anthony Hamzah Arif Wibowo Armaini, Armaini Arum Sekar Priyati Baehaqi Bela Safitri Wahyudi Besri Nasrul Desita Salbiah Eka Suryani Elza Zuhry Erlida Ariani Fajar Aulia Rahman Fauza Azmimelwa Fifi Puspita Fifi Puspita Fifi Puspita Haj Ali, Mayadah A. Hanifa Febriani Hapsoh Hapsoh Hapsoh Harry Firmansyah Idwar, Idwar Irfandri Irfandri Irfandri Irfandri Isna Dini Isna Rahma Dini Kausar Kausar Masjudi, Heri Muhammad Ali MUHAMMAD ALI Muhammad Ali Muhammad Ammar Huseini Novita Sari Depari Nurul Fadhila Ramadhona Nurwijayanti Pancasona, Muhammad Garda Pradhana, Adhi Putri Khairani Putro, Ananda Restu Rifa Shovia Riska JH Jundiah Shalihah, Filzah Sri Yoseva Sri Yoseva Surwadinata, Antama Titania Tjandrawati Nugroho Triwidodo Arwiyanto Yetti Elfina Yetti Elfina