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Pemanfaatan Aeromonas hydrophila Untuk Mengendalikan Penyakit Layu Fusarium Pada Beberapa Varietas Sorgum Manis (Sorghum bicolor (L.) Moench I WAYAN GENTA ARIAWAN; DEWA NGURAH SUPRAPTA; NI WAYAN SUNITI
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.4, No.2, April 2015
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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Abstract

The Use of Aeromonas hydrophila to Control Fusarium Wilt Disease on Several Varieties of Sweet Sorghum (Sorghum bicolor (L.) Moench) Objective of this study was to test the effectiveness of A. hydrophila to control wilt disease caused by Fusarium sp. on several varieties of sweet sorghum. Results of this study showed that application of rhizobacteria affected the growth and the yield of sweet sorghum. Under in vitro condition, treatments with A. hydrophila isolate KtBlt2 could suppress the growth of the Fusarium sp. with inhibitory activity 76.06% when compared to control. Under field condition treatment P6 (treatment wilt A. hydrophila on variety FS-501) could suppress Fusarium wilt disease with the lowest disease incidence by 3.24%. It is necessary to test the stability of A. hydrophila as biocontrol agent against Fusarium sp. Keywords : A. hydrophila, Fusarium sp., sweet sorghum
Uji Efektivitas Ekstrak Daun Cengkeh (Syzygium Aromaticum L.) Terhadap Phytophthora Palmivora Penyebab Penyakit Busuk Buah Kakao (Theobroma Cacao L.) NI LUH PUTU SONIA SAVITA DEWI; DEWA NGURAH SUPRAPTA; I KETUT SUADA
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.8, No.4, Oktober 2019
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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Abstract

Effectiveness of Clove Leaf Extract (Syzygium aromaticum L.) againstPhytophthora palmivora the Cause of Cocoa Fruit Rot (Theobroma cacao L.) Cocoa fruit rot caused by Phytophthora palmivora is one of the main diseases that can greatly reduce the yield of cocoa production in the world. Therefore it isnecessary to control the disease in an environmentally friendly way. One of which isusing botanical pesticides. Clove leaf is one of the plants which extract can be usedas a fungicide. This study aimed to examine the effectiveness of clove leaf extract onP. palmivora the cause of cocoa fruit rot. MIC test and colony test was done using 10extract concentrations namely 0,1; 0,2; 0,3; 0,4; 0,5; 0,6; 0,7; 0,8; 0,9; 1,0 and 0,0%(control). The in vivo test uses 6 extract concentrations of 0,5; 0,6; 0,7; 0,8; 0,9; 1,0and 0,0% (control). The results showed the MIC (minimum inhibitory concentration)of clove leaf extract to suppress the growth of P. palmivora was 0.5% which means itis feasible to be used as a botanical pesticide. Under in vivo condition, clove leaf extractinhibited the growth of fungal colonies and infection and can be used as fungicide tosuppress the growth of P. palmivora. The extract concentration of 1% prevented thedamage of cocoa fruit caused by P. palmivora by 100%.
Uji Aktivitas Antijamur Ekstrak Biji Keben (Barringtonia asiatica (L.) Kurz) terhadap Curvularia verruculosa Penyebab Penyakit Bercak Curvularia pada Tanaman Padi (Oryza sativa L.) I GEDE KARTA SATRIA WIBAWA; DEWA NGURAH SUPRAPTA; KHAMDAN KHALIMI
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.7, No.3, Juli 2018
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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Abstract

Antifungal Activity of Keben (Barringtonia asiatica (L.) Kurz) Seed Extract against Curvularia verruculosa the Causal Agent of Curvularia Leaf Spot Disease on Rice (Oryza sativa L.)This research was conducted to determine the effectiveness of keben (Barringtonia asiatica (L.) Kurz) seed extract to control Curvularia verruculosa the causal agent of curvularia leaf spot disease on rice. The extract concentration of 0.1%, 0.2%, 0.3%, 0.4%, 0.5%, 0.6%, 0.7%, 0.8%, 0.9% and 1.0% was tested on in vitro test to determine the minimum inhibitory concentration (MIC) and inhibition percentage. Crude seed extract was partitioned and analyzed using GC-MS to determine the active antifungal compound. Concentration of 0.6%, 0.7%, 0.8%, 0.9% and 1.0% is tested on in vivo test in a greenhouse to determine the effectiveness of keben seed extract formula to control the curvularia leaf spot disease on rice. The result shows that the MIC of keben seed extract was 0.5% with inhibitory percentage that increases from 0.1% to 0.5% and cause 100% inhibitory on 0.6% concentration and above. GC-MS analysis showed antifungal compound such as Butyl Hydroxy Toluene; Dodecanoic acid, methyl ester; Methyl tetradecanoate; Benzoic acid 2, 5-bis (trimethylsiloxy)-trimethylsilyl ester; 1-Tetradecanol; Hexadecanoic acid, methyl ester; Octasiloxane, 1, 1, 3, 3, 5, 5, 7, 7, 9, 9, 11, 11, 13, 13, 15, 15-hexadecamethyl-; 13-Docosenoic acid, methyl ester (Z)- and 1, 2-Benzenedicarboxylic acid, dioctyl ester are present in keben seed. Greenhouse test showed that keben seed extract can suppress curvularia leaf spot disease from 25.16% to 2.57%.
Uji Efektivitas Agen Hayati Dari Rizosfer dan Filosfer Tanaman Solanaceae untuk Mengendalikan Penyakit Antraknosa pada Tanaman Cabai Rawit (Capsicum frutescens L.) HENRI PAN BAGUS MULIA NAPITUPULU; KHAMDAN KHALIMI; DEWA NGURAH SUPRAPTA
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.9, No.1, Januari 2020
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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Effectiveness of biological agents from Solanaceae plants rhizosphere andfilosphere to control Antrachnose disease in Chili Pepper Plants (Capsicum frutescens L.). The disease antrachnose caused by the fungus Colletotrichum truncatum canattack the chili fruit at any stage, but it will not attack the leaves or stems. The use ofbiological agents from the rhizosphere and filosphere is a viable alternative to controlthe anthracnose disease. Biological agents work selectively and better for theecosystem. The goal of this research is to identify the effectivenes of biological agentsfrom rhizospheres and filospheres solanaceae plant to control C. truncatum that causesantrachnose disease in Chili Pepper Plants (Capsicum frutescens L.). This researchtakes place in vitro and the field this research shows that TmFr4 and TrRr7 treatmentcan inhibit the growth of C. truncatum in vitro with a high percentage of inhibition90,58 % and 91,23 %. The TrRr7 bacterial isolate can inhibit the anthracnose diseasefrom 95,23% to 64,23 % and able to reduce the intensity from 66,59 % tob 39,06 %while TmFr4 fungal isolates can inhibit the desiase from 95,23 % to 68,81 % andreduce its intensity from 66,59 % to 47,03 % base on the field test. Further study isneeded to analyze the stability of TmFr4 fungal isolate and TrRr7 bacterial isolatebiological control of C. truncatum in the field.
Uji Antagonistik Bacillus siamensis dan Paenibacillus polymyxa Terhadap Colletotrichum gloeosporioides KLCR2 Penyebab Penyakit Antraknosa pada Buah Cabai Rawit (Capsicum frutescens L.) NI KOMANG SRI BAWANTARI; DEWA NGURAH SUPRAPTA; KHAMDAN KHALIMI
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.9, No.3, Juli 2020
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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Abstract

Antagonistic test of Bacillus siamensis and Paenibacillus polymyxa against the growth of Colletotrichum gloeosporioides the cause of Antracnose disease in cayenne pepper (Capsicum frutescent L.) Colletorichum gloeosporioides is one of latent pathogens that can infect several agricultural crops. This pathogenic fungus is very detrimental to the production of cayenne pepper (Capsicum frutescens L.). The purpose of this research was to determine the ability of Bacillus siamensi and Paenibacillus polymyxa in inhibiting the growth of C. gloeosporioides KLCR2 causes of anaplosal disease. Research was conducted in Biopesticide laboratory. Results showed that treatment with antagonistic bacteria significantly (P<0.05) inhibited the growth of C. gloeosporioides on potato dextrose agar (PDA) medium. Treatment with B. siamensis was able to inhibit the growth of C. gloeosporioides with inhibitory activity by 96.18%. While for the treatment with P.polymyxa the inhibitory activity was 84.79%. Treatment with B. siamensi depressed the biomass of C. gloeosporioides KLCR2. In this study, treatment with cell-free filtrate of B. siamensis at concentration of 50% was able to suppress the development of C. gloeosporioides with a percentage of inhibitory activity by 94.15%. Further study is necessary to evaluate the ability of the B. siamensis to inhibit the development of C. gloeosporioides KLCR2 in vivo on cayenne pepper fruit.
Studi Komponen Bioaktif Asparagus (Asparagus offcinalis) dan Potensinya sebagai Antioksidan Agus Selamet Duniaji; D. N. Suprapta; NN Puspawati; I B Yoga
Jurnal Ilmiah Teknologi Pertanian Agrotechno Vol 1 No 1 (2016)
Publisher : Universitas Udayana

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Abstract

Asparagus adalah sayuran yang telah lama digunakan sebagai makanan karena rasanya lezat dan sifatdiuretik. Sebagai sifat diuretik, asparagus diyakini mampu memperbaiki saluran kemih sehingga dapatmeningkatkan kinerja ginjal. Tujuan dari penelitian ini adalah untuk menentukan komponen bioaktif dariasparagus yang dibudidayakan di Bali. Penelitian ini dilakukan pada 5 jenis asparagus segar terdiri dari 2sampel dari petani, 1 sampel yang diambil di supermarket dan dua sampel asparagus dari Malang danMedan yang diperoleh di ACS (Aero Catering Service Ngurah Rai Tuban). Penelitian ini diulangsebanyak 3 kali. Data yang diperoleh dianalisis secara deskriptif dengan menampilkan nilai rata-rata danstandar deviasi. Hasil penelitian menunjukkan bahwa kandungan klorofil tertinggi asparagus adalah 9.28mg kg-1bb, Total fenol adalah 285,22 mg GAE / 100g bb dan komponen bioaktif untuk 5 sampelberpotensi sebagai antioksidan karena dapat mengurangi senyawa radikal dengan adanya klorofil dansenyawa fenolik. Kandungan klorofil terendah ada pada asparagus Malang yaitu sebesar 6.87 mgkg-1bbdan Total fenol adalah 243,12 mg GAE / 100g bb
UPAYA MEMINIMALISIR DAMPAK LINGKUNGAN DARI PENGGUNAAN PESTISIDA DALAM PERTANIAN (DAMPAK LINGKUNGAN DAN PENANGGULANGANNYA) Hadis Jayanti; Dewa Ngurah Suprapta
AGRICA Vol. 2 No. 1 (2009): June
Publisher : Agriculture Faculty of Flores University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37478/agr.v2i1.541

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Utilization of synthetic pesticide seems up to now remains to be one of the methods in controlling pest and plant disease because it plays a role in determining the quality and quantity of the crops. Improver of synthetic pesticide in agriculture can adversely affect the ecosystem such as water and soil pollution. The occurrence of pest resistance, the outbreak of secondary pest, the resurgence of pest, the death of predators, the residue of pesticide in food, and the pesticide residue that affects man's health. Although it has been widely known that pesticide has a bad impact on the environment, the use of pesticide is still needed in agricultural practice. Its use needs to pay attention to the concept of Integrated Pest Management in which the use of pesticide is only made when it is absolutely necessary, that is, when the population of pest increases and lies above one level of the pest population, the Economic Threshold (Untung, 1993). To know the safety level of pesticide, evaluation of toxicity of a pesticide is needed and it is commonly tested in the animal of high level. This is done in such a way that the toxicity and safety level of the pesticide can be known by farmers, users of pesticide, and the environment. Based on the information on the toxicity level, it is expected that the steps of environmental management can be taken to minimize the negative impact of pesticide. The effort of minimizing the negative impact of pesticide can be done in a variety of ways. For example, the direction was given to the users in terms of giving the dosage, application time, safe working procedure, choosing pesticide with the precise target, choosing a pesticide that can decompose easily, application target, a time before harvest, tool and exact application technique, keeping as small pollution as possible. In the future, it is hoped that the use of pesticide will decrease and will be more selective, and supported by new inventions in the technology of processing pesticide disposal and the technology of utilizing botanical pesticide which is all expected to be effective in overcoming the disturbance of pest and plant disease at the same time minimizing environmental degradation.
Uji Efektivitas Ekstrak Buah Andaliman (Zanthoxylum acanthopodium. D.C) Terhadap Phytophthora palmivora Penyebab Penyakit Busuk Buah Kakao (Theobroma cacao L.) BERTA BR GINTING; DEWA NGURAH SUPRAPTA; NI WAYAN SUNITI
Agrotrop : Journal on Agriculture Science Vol 12 No 1 (2022)
Publisher : Fakultas Pertanian Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/AJoAS.2022.v12.i01.p08

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Effectiveness Test of Andaliman Fruit Extract (Zanthoxylum acanthopodium. D.C) Against Phytophthora palmivora the Cause of Cocoa (Theobroma cacao L.) pod rot disease. Fruit rot disease caused by the fungus Phytophthora palmivora causes the loss of crop yields up to 90%. Therefore, an effort is needed to control Phytophthora palmivora that causes blight in cocoa plantations. Alternative that can be done is the use of botanical fungicides. One of the plants that can be used as a botanical fungicide is the fruit of Andaliman (Zanthoxylum acanthopodium D.C). This study aims to test the effectiveness of andaliman fruit extract against P. palmivora which causes pod rot disease. Colony test and cocoa pod damage test in vivo using extract concentrations, namely 50%; 25%; 16.7%; and 0% (control). The results showed that andaliman fruit extract could inhibit the growth of P. palmivora which can be used as botanical fungicide. The concentration of andaliman fruit extract 50% and 25% can reduce cocoa pod damage caused by P. palmivora by 58% and 45.7%, respectively.
Uji Efektivitas Ekstrak Daun Awar-Awar (Ficus Septica Burm F) untuk Menghambat Pertumbuhan Jamur Phytophthora infestans Penyebab Penyakit Hawar Daun Tomat NOVA SULIYANTI SIANTURI; DEWA NGURAH SUPRAPTA; NI WAYAN SUNITI
Agrotrop : Journal on Agriculture Science Vol 13 No 1 (2023)
Publisher : Fakultas Pertanian Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/AJoAS.2023.v13.i01.p05

Abstract

Effectiveness of Awar-Awar (Ficus septica Burm F) Leaf Extract to Inhibit the Growth of the Fungus Phytophthora infestans that Causes Tomato Late Blight. Tomato late blight caused by the fungus Phytophthora infestans is one of the main diseases affecting tomato production in the world. Therefore, it needs to be controlled in an environmentally friendly way, one of which is by using vegetable pesticides. Awar-awar leaves are one of the plants whose extracts can be used as vegetable fungicides. This study aimed to examine the potency of awar-awar leaf extract against P. infestans which causes late blight of tomatoes. MIC test and colony test used 10 extract concentrations, namely 0.1%; 0.2%; 0.3%; 0.4%; 0.5%; 0.6%; 0.7%: 0.8%; 0.9%; 1.0% and control. The in vivo test used 8 extract concentrations, namely 0.3%; 0.4%; 0.5%; 0.6%; 0.7%: 0.8%; 0.9%; 1.0% and control. The results showed that the Minimum Inhibitory Concentration (MIC) of awar-awar leaf extract to suppress the growth of P. infestans was 0.3%, which means that it is suitable for use as a vegetable pesticide. Colony test results showed that awar-awar leaf extract was able to inhibit the growth of P. infestans fungal colonies. In vivo awar-awar leaf extract inhibits the growth of fungal colonies and the occurrence of infection, and can be fungicidal to suppress the growth of P. infestans. Extract concentration of 1% can suppress tomato leaf damage by P. infestans by 75.90%.
Uji Efektivitas Ekstrak Daun Trembesi (Samanea saman (Jacq.) Merr) Untuk Menghambat Pertubuhan Jamur Colletotrichum sp. Penyebab Penyakit Antraknosa Pada Pepaya (Carica Papaya) ALPIN JUAN HAGATA PINEM; DEWA NGURAH SUPRAPTA; NI WAYAN SUNITI
Agrotrop : Journal on Agriculture Science Vol 13 No 2 (2023)
Publisher : Fakultas Pertanian Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/AJoAS.2023.v13.i02.p05

Abstract

Effectiveness of Rain Tree Leaf Extract (Samanea Saman (Jacq.) Merr) to Inhibit the Growth of the Fungus Colletotrichum sp. the Cause of Anthracnose Disease on Papaya (Carica papaya). Due to its high nutritional value and high economic value, papaya is a local fruit that is in great demand in the community. The production of papaya fruit fluctuates due to a number of factors, one of which is the fungus Colletotrichum sp., which causes anthracnose disease in papaya fruit. In most cases, synthetic fungicides are utilized to combat anthracnose. Reduce the use of synthetic fungicides and replace them with more environmentally friendly plant-based fungicides, such as those derived from plant extracts. This study aimed to determine the efficacy of rain tree (Samanea saman) leaf extract in inhibiting and controlling the growth of the anthracnose-causing fungus Colletotrichum sp. in papaya fruit. In vitro and in vivo evaluation of the efficacy of trembesi leaf extract against the fungus Colletotrichum sp. The minimum inhibitory concentration (MIC) of trembesi leaf extract to inhibit the growth of Colletotrichum sp. was determined to be 0,1%. The results of the colony test demonstrated that the trembesi leaf extract inhibited the growth of Colletotrichum sp. colonies. In vivo inhibition tests revealed that trembesi leaf extract could inhibit the growth of fungal colonies, prevent infection, and suppress the growth of Colletotrichum sp. The 2,5% extract concentration can prevent 66,22% of the damage caused by Colletotrichum sp. to papaya fruit.