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All Journal Agrotrop : Journal on Agriculture Science journal of internal medicine AGRITROP Journal of Agricultural Science and Biotechnology Jurnal Veteriner Bumi Lestari Ecotrophic, Journal of Environmental Science E-Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Biogenesis: Jurnal Ilmiah Biologi Jurnal Ilmiah Teknologi Pertanian Agrotechno MNJ (Malang Neurology Journal) JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Agrica: Journal of Sustainable Dryland Agriculture
Dewa Ngurah Suprapta
Program Studi Agroekoteknologi Fakultas Pertanian Universitas Udayana
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Earth & Planetary Sciences Energy Environmental Science Immunology & microbiology Medicine & Pharmacology Neuroscience Veterinary

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UJI ANTAGONIS BAKTERI ENDOFIT TERHADAP Colletotrichum scovillei PENYEBAB PENYAKIT ANTRAKSONA PADA CABAI BESAR (Capsicum annuum L.) I Gede Karta Satria Wibawa; Dewa Ngurah Suprapta; Khamdan Khalimi
Journal of Agricultural Science and Biotechnology Vol 8 No 1 (2019)
Publisher : Journal of Agricultural Science and Biotechnology

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Abstract

Colletotrichum scovillei is a newly discovered species causing anthracnose disease on chilli pepper (Capsicum annuum L.) in Bali. With the increasing reports of Colletotricum spp. resistance against chemical fungicide, biological control is taken as the alternatives to control this disease. In this study a total of 69 endophytic bacteria isolates was recovered from different solanaceae plants samples collected from nine regions of Bali and screened for the antagonism againstC. scovillei. From the potential 15 candidates of isolates with inhibition activity above 90%, hypersensitivity test were taken on tobacco leaf, and isolate CBCKB1 was chosen for further test. The CBCKB1 isolate was identified as Bacillus amyloliquefaciens based on molecular identification. Detached fruit bioassay proved that isolate CBCKB1 formula was effective in reducing the disease percentage and disease severity of anthracnose disease on chilli pepper.
POTENSI RIZOBAKTERI YANG DIISOLASI DARI RIZOSFIR TANAMAN GRAMINAE NON-PADI UNTUK MEMACU PERTUMBUHAN BIBIT PADI Ni Made Intan Maulina; Khamdan Khalimi; Gusti Ngurah Alit Susanta Wirya; Dewa Ngurah Suprapta
Journal of Agricultural Science and Biotechnology Vol 4 No 1 (2015)
Publisher : Journal of Agricultural Science and Biotechnology

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Abstract

Rice (Oriza sativa L.) is the main food for Indonesian people, however the rice production is not enough to fulfill all national demand. Rice productivity in Indonesia was about 4,5 ton/ha, relatively low when compared to other rice growing countries such Australia, Japan, and China. It is necessary to find a measure to increase the rice productivity in Indonesia. One of the Measure is the use of rhizobacteria isolated from the rhizozpheres of the plants belongs to the family Graminae. This study was done in order to find potential rhizobacteria from rhizospheres of five plant species belong to the family Graminae, namely Cymbopogon ciatrus, Imperata cylindrica, Saccharum officinarum, Pennisetum purpureum and Schizostachum mosum. Result of this study showed that 10 isolates of rhizobacteria obviously increased the number of lateral roots of rice seedling cultivar Ciherang. The rice seedling treated with isolate Al7Kla produced the highest number of lateral roots when compared to other isolates. This isolate may be used to promote the rice growth in order to increase the yield.
THE EXTRACT OF PURPLE SWEET POTATO DECREASE BLOOD AND LIVER MDA OF MICE AFTER INTENSE PHYSICAL ACTIVITY I Made Jawi; Dewa Ngurah Suprapta; A.A. Ngurah Subawa
Jurnal Veteriner Vol 9 No 2 (2008)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Oxidative stress as consequence of intense physical exercises cause tissue lesions. The objective of this study was to identify the comparable antioxidant effect of water extract and syrup of Balinese purple sweet potato to the liver tissue in mice after maximal exercise, through the quantification of blood and liver malondialdehyde (MDA), and the histologic feature of liver tissue. Subjects of this study were 60 adult male Swiss mice divided into 6 groups with control group post-test only design. Blood and liver were collected after exercise protocol of exhausting swimming in ten mice without water extract, ten mice with water extract and ten mice with syrup of Balinese purple sweet potato. The MDA was quantification with TBARS method. Samples were also collected from control group of ten mice without water extract, ten mice with water extract and ten mice with syrup of Balinese purple sweet potato as sedentary mice. The results show a significant increase of MDA in the blood and liver, after swimming with and without water extract or syrup of Balinese purple sweet potato respectively (p<0,05). The increase of MDA was slightly significant in the group with water extract or syrup of Balinese purple sweet potato (p<0,05). The increase of liver cell necrosis was slightly significant in the group with water extract of Balinese purple sweet potato (p<0,05). From this finding it can be concluded that water extract or syrup of Balinese purple sweet potato has antioxidant effect in exhausting exercise in mice. Water extract of Balinese purple sweet potato has protective effect to the liver cell in exhausting exercise in mice
Bioaktivitas Forbazol-E terhadap Kerusakan Ultrastruktur Dinding Sel Staphylococcus aureus Ni Putu Ristiati; Ketut Suata; Dewa Ngurah Suprapta
Jurnal Veteriner Vol 10 No 4 (2009)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The bioactivity role of phorbazol-E in inhibiting bacterial protein synthesis is unknown. Onthe contrary, phorbazol-A, B, C, and D have been proved to inhibit the bacterial protein synthesis.The effect of forbazol-E on Staphylococcus aureus ATCC25922 cell wall ultrasctucture destructionwas observed. The study used randomized-post test-only control group design which consist of threetreatment (control, treatment I and II) with 9 repeatation. Treatment I: 1 ml overnight culture ofS. aureus was cultured into a mixed of 20 ml Mueller Hinton (MH) broth and 37.5 mg/L phorbazol-E; whereas in treatment II phorbazol-E used was 75.0mg/L. The growth curve of S. aureus wasmonitored using spectrophotometer; whilst the destruction of cell wall ultrastructure was observedusing Transmission Electron Microscope (TEM) at Eijkman Insitute, Jakarta. Phorbazol-E at75.0mg/L significantly caused the destruction of the bacteria cell wall ultrastructure and inhibitedthe bacteria growth in comparison to phorbazol-E at 37.5 mg/L (p<0.05).
APLIKASI POLYMERASE CHAIN REACTION-RIBOSOMAL INTERGENIC SPACER ANALYSIS (PCR-RISA) UNTUK MENENTUKAN KERAGAMAN MIKROBA TANAH PADA HABITAT TANAMAN PISANG DENGAN DAN TANPA GEJALA LAYU FUSARIUM I Made Sudarma; Dewa Ngurah Suprapta; I Made Sudana; I Gede Rai Maya Temaja
Bumi Lestari Journal of Environment Vol 12 No 2 (2012)
Publisher : Environmental Research Center (PPLH) of Udayana University

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Abstract

A major problem in the soil microbiology is 99% soil microbes could not be grown in artificial media; therefore, to describe the true diversity of microbes in the soil must use a molecular approach. The approach was polymerase chain reaction-ribosomal intergenic spacer analysis (PCR-RISA). This method can be used for determining soil microbial DNA profile (the number of DNA bands). The soil samples were collected from three regencies in Bali,  i.e. Karangasem, Klungkung and Jembrana which are the main banana growing area in Bali. Soil sampling was done in two sites in each regency representing the banana habitat with and without Fusarium wilt symptom, by collecting 100 grams of soil surrounding the banana plant at the depth of 20 cm. The result of PCR-RISA showed that DNA profile of the soil microbes in the soil of banana habitat without Fusarium wilt symptom (HN) more than of the soil banana habitat with Fusarium wilt symptom (HF). DNA profiles at HN there were three, each of the indicated 450 bp, 250 bp and <100 bp, whereas in HF were two bands of DNA, each indicated as 250 bp and <100 bp. Based on the number of DNA bands proved that the soil microbial community in the HN is higher than in HF.
RESIDU PESTISIDA PADA SAYURAN KUBIS (Brassica oleracea L.) DAN KACANG PANJANG ( Vigna sinensis L.) YANG DIPASARKAN DI PASAR BADUNG DENPASAR K Agung Sudewa; D N Suprapta; Dan M S Mahendra
ECOTROPHIC : Jurnal Ilmu Lingkungan (Journal of Environmental Science) Vol 4 No 2
Publisher : Master Program of Environmental Science, Postgraduate Program of Udayana University

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Abstract

Pesticides residue of organophosphate and carbamate i.e. diazinon, chlorpyriphos, fentoate, carbaril and BPMC were tested on cabbage (Brassica oleracea L.) and long bean (Vigna sinensis L.. The purpose of this study was to know the level of pesticides residue remaining on cabbage and long bean marketed in Badung Market, Denpasar.The samples were determined proportionally based on purposive sampling method. The proportion of sample was 10% of the total cabbage and snake bean sold in Badung market.Result of present study showed that residue of insecticides such as diazinon, chlorpyriphos, fentoate, carbaril, and BPMC remaining on the head of cabbage and snake bean marketed in Badung market was affected by the frequencies of their use in the field, in which chlorpyriphos was used by 60-65% of the farmers and carbaril by 40% of the farmers. Their residues on cabbage anf snake bean were 0.525 ppm and 1.296 ppm for chlorpyriphos (organophosphate); 0.303 ppm and 0.471 ppm for carbaril (carbamate). These result suggested that residue of chlorpyriphos on cabbage and snake bean were higher than MRL (Maximum Residue Limit) for vegetable crops, i.e. 0.5 ppm.
KERAGAMAN MIKOFLORA TANAH PADA HABITAT TANAMAN PISANG DI BALI l MADE SUDARMA; D.N. SUPRAPTA; Rai Maya Temaja
ECOTROPHIC : Jurnal Ilmu Lingkungan (Journal of Environmental Science) Vol 6 No 1 (2011)
Publisher : Master Program of Environmental Science, Postgraduate Program of Udayana University

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Abstract

Fungi in the soil plays an important role in maintaining the health and quality ofland, one of several indicators of soil health that is the diversity of soil fungi. This study was done in order to know the soil fungi diversity in the soil of banana plants habitat. The soil samples were collected from three regencies in Bali, i.e. Karangasem, Klungkung and Jembrana which are the main banana growing areas in Bali. Soil sampling was done in two sites in each regency, by collecting 100 grams of soil surrounding the banana plant at the depth of 20 cm, with three replication. Soil microbes population density particularly for bacteria, fungi and actinomycetes were determined based on plate account technique, while the microbes diversity was determined based on Diversity Index of Shannon-Wiener. Diversity index of soil fungi of all soil samples ranged from 0.8785 to 2.1458 (criteria oflow to moderate), with population densities ranging from 1.1 x 104 to 2.8 x 104 cfu / g soil. Evenness index at all sites soil samples obtained ranged from 0.6688 to 0.9766, this means the fungus species found there are no outstanding domination. Similarity index on all soil samples showed less than 0.5, which means one does not have a kinship with each other. Physicochemical factors that affect population density of soil fungi on the banana plant habitats: organic C, total N, available P, available K, soil moisture content (air dry capacity and field capacity), sand and clay. W hile soil physicochemical factors that influence the number of species (diversity) of soil fungi namely: C-organic content, total N and the dust has positive influence on the number of species in banana plants habitat, whereas soil sand content negatively affected the number of types of soil fungi.
Uji Efektivitas Fungisida Alami dan Sintetis dalam Mengendalikan Penyakit Layu Fusarium pada Tanaman Tomat yang Disebabkan oleh Fusarium oxysporum f.sp. lycopersici LASTRI APRIANI; DEWA NGURAH SUPRAPTA; I GEDE RAI MAYA TEMAJA
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.3, No.3, Juli 2014
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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Abstract

ABSTRACT The Test of Natural and Synthetic Fungicides Effectiveness in Controlling Fusarium Wilt of Tomato Plants Caused by Fusarium oxysporum f.sp. lycopersici Fusarium wilt caused by Fusarium oxysporum f.sp. lycopersici is one of important diseases in tomato plants. The emersion of the disease can result in huge loss for farmers. The use of synthetic fungicides that has been widely utilized all this time by farmers to control diseases caused by Fusarium oxysporum f.sp. lycopersici has adverse environmental impacts, therefore it is required biological fungicide that is more environmentally friendly. This study is purposed to testing the effectiveness of some natural fungicides and synthetic fungicide, is like a betel leaf, Trichoderma harzianum fungus, Klebsiella pneumoniae, and benomyl for suppress F. oxysporum f.sp. lycopersici and restrained Fusarium wilt diseases . The result of this study shows that the biological fungicide of betel leaf extract can inhibit the pathogenic F. oxysporum f.sp. lycopersici by 76.11 % and the percentage of inhibition potency of other benomyl fungicides reaches 61.11 % , while Klebsiella pneumoniae is by 54.42 % , and 20.98 % for Trichoderma harzianum on PDA medium. The result of field test shows that the extract of betel leaf can suppress Fusarium wilt by 5 % , T. harzianum , benomyl and K. pneumoniae are able to suppress Fusarium wilt respectively by 2.5 %. The use of T. harzianum and K. pneumoniae as biological control has the same effectiveness with synthetic fungicide in order to suppress Fusarium wilt.   Keywords: fusarium wilt, fusarium oxysporum f.sp. lycopersici, biological fungicide
Uji Keefektifan Rizobakteri dalam Menghambat PertumbuhanJamurColletotrichum spp. Penyebab Antraknosa pada Cabai Merah (Capsicum annuum L.) NI LUH GEDE SRIYANTI; DEWA NGURAH SUPRAPTA; I KETUT SUADA
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.4, No.1, Januari 2015
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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Abstract

ABSTRACT Effectiveness of Rhizobacteria to Inhibit the Growth of Colletotrichum spp. the Cause of Antracnose on Red Chilli (Capsicum annuum L.) Colletotrichumspp. is one of pathogenic fungi that cause significant losses on red chilli. The use of rhizobacteria is considered as an alternative to control the growth of the fungus. The use of bio agent control is considered as selective and environmentally friendly. The aim of this study is to know the effectiveness of rhizobacteria and their filtrates in suppressing the growth of Colletotrichumspp. Results of this study showed that treatment with Pseudomonas fluorescens isolate TBZA on PDA medium could suppress the growth of Colletotrichumspp. by 94% when compared to control 14 days after inoculation. Treatment with filtrate of P. fluorescens isolate TBZA could suppress the anthracnose disease indicated by the lower disease incidence viz 32%. It is necessary to conduct further experiment under field condition to know the effectiveness of P. fluorescens isolate TBZA to suppress the growth of Colletotrichumspp. In addition isolation and identification of antifungal compounds in the filtrate is necessary to be done. Keywords: rhizobacteria, biologicalagents, and Colletotrichumspp.
Uji Aktivitas Antijamur Bacillus siamensis C7B terhadap Jamur Colletotrichum scovillei Penyebab Penyakit Antraknosa pada Tanaman Cabai Besar (Capsicum annuum L.) PARDIANTA PATRISIUS SINAGA; KHAMDAN KHALIMI; DEWA NGURAH SUPRAPTA
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.9, No.4, Oktober 2020
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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Abstract

Test of Antifungal Activity of Bacillus siamensis C7B Against Fungus Colletotrichum scovillei Causing Anthracnose Disease on Chili Pepper (Capsicum annuum L.) Colletotrichum scovillei is one of the species of fungi that causes anthracnose disease on chili peppers. The Purpose of this study was to determine the potential of B. siamensis C7B in inhibiting the growth of the fungus C. scovillei that causes anthracnose disease in large chili (C. annuum L.). This study was conducted in vitro by testing the antifungal activity of Bacillus siamensis C7B against three isolats of C. scovillei namely TBCR, SGCR, and GRCR on potato dextrose agar (PDA) and potato dextrose broth (PDB) media. The test results indicate that inhibition of the bacteria B. siamensis C7B able to inhibit the growth of fungi isolates of C. scovillei namely TBCR, SGCR, and GRCR with the percentage of inhibition respectively by 94,91%, 92,66%, and 91,47% when compared to the control on the observation of 21 days after inoculation. The inhibition test results of B. siamensis C7B filtrate at a concentration of 50% showed that the B. siamensis C7B filtrate was able to inhibit the growth of C. scovillei TBCR isolates with a percentage of inhibition activity of 90,27% when compared to control. The results of inhibition test of B. siamensis C7B on the formation of C. scovillei biomass of isolate TBCR showed that B. siamensis C7B was able to inhibit the formation of fungal biomass by 73,81% when compared to control. Further study is needed to evaluate the effectiveness of B. siamensis C7B to control anthracnose disease in vivo on chili pepper.
Co-Authors Agung Bagus S. Satyarsa Agung Wiwiek Indrayan Agus Selamet Duniaji ALPIN JUAN HAGATA PINEM Anak Agung Ngurah Subawa BERTA BR GINTING Emilia Simpllisiu Ake Wangge Gitari, Ni Made Gusti Ngurah Alit Susanta Wirya Hadis Jayanti HENRI PAN BAGUS MULIA NAPITUPULU I B Yoga I Dewa Made Sukrama I G. R. M. TEMAJA I GEDE KARTA SATRIA WIBAWA I Gede Karta Satria Wibawa I Ketut Suada I MADE ADNYANA I Made Jawi I Made Sudana I MADE SUDARMA I Putu Eka Widyadharma I WAYAN GENTA ARIAWAN I Wayan Niryana I Wayan Sumardika Jayanti , Hadis K Agung Sudewa Ketut Suata Khamdan Khalimi Komang Adi Mahartha l MADE SUDARMA LASTRI APRIANI Leana, Ni Wayan Anik Losen Adnyan M. Sudiana Mahendra Made Agus Hendrayana MADE SUSUN PARWANAYONI Meitini Proborini Wahyuni Ni Kadek Desy Andya Dewi NI KOMANG SRI BAWANTARI NI LUH GEDE SRIYANTI NI LUH PUTU SONIA SAVITA DEWI Ni Made Intan Maulina Ni Nyoman Puspawati Ni Putu Ristiati NI WAYAN SUNITI NOVA SULIYANTI SIANTURI Nyoman Darsini PARDIANTA PATRISIUS SINAGA Raka-Sudewi A. A. SANG KETUT SUDIRGA Sanjaya, Feliani Sukmawati Sukmawati Sukmawati Sukmawati Thomas Eko Purwata Unedo, Dion Valente Quintao