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All Journal Jurnal Teknologi Dan Industri Pangan Jurnal Penelitian Saintek EKSAKTA: Journal of Sciences and Data Analysis Reaktor Indonesian Journal of Biotechnology Indonesia Chimica Acta Indonesian Journal of Chemistry Jurnal Teknik Terapan (J-TETA) Molekul: Jurnal Ilmiah Kimia
Tutik Dwi Wahyuningsih
Department Of Chemistry, Faculty Of Mathematics And Natural Sciences, Universitas Gadjah Mada, Jl. Sekip Utara, Yogyakarta 55281
Agriculture, Biological Sciences & Forestry Automotive Engineering Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Control & Systems Engineering Decision Sciences, Operations Research & Management Earth & Planetary Sciences Electrical & Electronics Engineering Energy Immunology & microbiology Industrial & Manufacturing Engineering Materials Science & Nanotechnology Mechanical Engineering

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Antibacterial Peptide from Chymotrypsin Hydrolysate of Jatropha Seeds with RP-HPLC Fractionation Andriana, Zehan; Wahyuningsih, Tutik Dwi; Raharjo, Tri Joko
Molekul Vol 18 No 2 (2023)
Publisher : Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.jm.2023.18.2.7080

Abstract

Antimicrobial peptide (AMP), as a new antibiotic agent, has promising prospects in overcoming the problem of resistance. AMP production can be carried out by proteolytic enzymes. Protein from castor bean (R. communis) is toxic, so it can potentially be a source of AMP. This study aims to obtain protein hydrolysate from the castor bean (R. communis) using several extraction methods, including SDS dialysis, SDS-gel filtration, trifluoroacetic acid (TFA), and acetone precipitation. Chymotrypsin enzyme was used to hydrolyze the protein, and the peptide hydrolysate was fractionated using RP-HPLC. The peptide fraction was tested for its antibacterial activity by agar diffusion and microdilution methods, and the most active fraction was identified for its amino acid sequence by LC-HRMS. The results showed that the acetone precipitation extraction method was the best method, with a degree of hydrolysis of 83.9%. The active fractions 6 and 10 of RP-HPLC had IC50 values of 14.1 and 14.5 µg/mL for E. coli and 13.3 and 14.4 µg/mL for S. aureus, respectively. NVLRGKGMASL peptides were found in fraction 10, and GIILLSSK, NMIAKR, and LLDILTKK peptides were found in fraction 6 with an alpha helix secondary structure that can cause membrane damage. The peptides NVLRGKGMASL, GIILLSSK, NMIAKR, and LLDILTKK are thought to have potential as antibacterial compounds.
In Silico Insight of Natural Compounds in Ageratum Conyzoides L as Anti Breast Cancer Candidate by Molecular Docking Against EGFR Tjitda, Putra Jiwamurwa Pama; Wahyuningsih, Tutik Dwi; Nitbani, Febri Odel
Jurnal Akta Kimia Indonesia (Indonesia Chimica Acta) Volume 17, No 2: December 2024
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.70561/ica.v17i2.35928

Abstract

The regulation of proliferation and apoptosis in cancer cells, particularly breast cancer, hinges on the control exerted by the epidermal growth factor receptor (EGFR). Naturally derived EGFR inhibitors from natural compounds show significant potential for future advancements. This study aims to assess the efficacy of active compounds found in Ageratum Conyzoides L as EGFR inhibitors. Evaluation involved molecular docking studies of flavonoid, chromene, terpene, sterol, and acid compounds against EGFR using PDB ID 1m17. Additionally, the ADMET properties and drug-like characteristics of the most promising compounds were characterized. Findings reveal that compound C2 (fisetin) exhibits the most favorable binding energy of -8.9 kcal/mol. Fisetin establishes crucial interactions, particularly with the catalytic site at Met765 of the hinge protein. Fisetin also engages in hydrophobic interactions in regions I and II, involving Val702, Leu694, and Leu820. ADMET profiling of fisetin demonstrates favorable attributes, suggesting its potential as a promising anticancer agent based on drug-likeness assessments.
Synthesis and Antibacterial Activity of N-phenyl-3-(4-chlorophenyl)-5-(3,4-dimethoxy-phenyl) pyrazoline Nofiyanti, Estin; Wahyuningsih, Tutik Dwi; BTAG, Noer Laelly; Anwar, Chairil; Nurjanah, Novi; Salman, Nurcholis; Priastomo, Yoga
Molekul Vol 20 No 2 (2025)
Publisher : Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.jm.2025.20.2.5618

Abstract

ABSTRACT. Compound of N-phenyl-3-(4-chlorophenyl)-5-(3,4-dimethoxy-phenyl) pyrazoline was synthesized by cycloaddition reaction. The structure of the product was chracterized by FTIR, 1H-NMR and 13C-NMR spectrometers. The pyrazoline was screened for antibacterial activity using agar diffusion method against Staphylococcus aureus, Bacillus cereus, Bacillus subtillis, Shigella flexnerri, and Escherichia coli. The pyrazoline had been synthesized from chalcone with phenylhydrazine to give 52.90% yield. Antibacterial screening showed that pyrazoline was active against selected gram positive bacteria. The pyrazoline was found to exhibits an antibacterial activity and its zone of inhibition/concentration against S. aureus (8.75/300), B. cereus (7.75/1000), B. subtillis (6.50/1000), and E. coli (3.50/300). The result showed that pyrazoline which was subtituted with chloro and methoxy, was able to posse broad spectrum of the tested pathogenic bacteria. Keywords: chalcone, pyrazoline, antibacterial
Pengaruh Katalis NaOH Terhadap Proses Metilasi Senyawa Brazilein Pada Ekstrak Kayu Secang (Caesalpinia sappan linn) Ulma, Zeni; Rahayuningsih, Edia; Wahyuningsih, Tutik Dwi
Jurnal Teknik Terapan Vol. 1 No. 1 (2022): April
Publisher : P3M Politeknik Negeri Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (668.22 KB) | DOI: 10.25047/jteta.v1i1.6

Abstract

Reaksi metilasi adalah reaksi penggantian atom atau molekul dengan gugus metil. Beberapa parameter yang mempengaruhi proses metilasi antara lain rasio bahan dan waktu metilasi, namun hasil metilasi tidak optimal. Pada penelitian ini dilakukan penambahan katalis pada proses metilasi. Penambahan katalis berpengaruh terhadap peningkatan stabilitas warna ekstrak secang terhadap perubahan pH. Variabel yang digunakan dalam penelitian ini adalah konsentrasi katalis 0; 0,002; 0,01; 0,02; dan 0,04 mol/mL. Parameter yang diuji meliputi stabilitas terhadap pH (2, 3, 5, 7, dan 10) dan degradasi warna terhadap oksidator. Konsentrasi katalis penambahan terbaik adalah pada kisaran 0,002 hingga 0,01 mol/mL. Selain itu, adanya katalis NaOH dalam proses metilasi ekstrak secang juga dapat mengurangi degradasi warna yang disebabkan oleh adanya oksidator.
Co-Authors Andriana, Zehan Bambang Purwono BTAG, Noer Laelly Chairil Anwar Chairil Anwar Chatarina Lilis Suryani Edia Rahayuningsih Elfi Susanti V. H. Estin Nofiyanti Hernawan Hernawan Lina Fauzi'ah Marit Kolb Müfit Bahadir Muhammad Idham Darussalam Mardjan Mustofa Mustofa Narsito Narsito Nosa Septiana Anindita Nurjanah, Novi Priastomo, Yoga Purwo Handoko, Donatus Setyawan Rahma Diyan Martha Retno Ambarwati Sabirin Matsjeh Salman, Nurcholis Samuel Budi Wardhana Supriyadi Supriyadi Taufik, Tiyas Tono Tiyas Tono Taufiq Tjitda, Putra Jiwamurwa Pama Tri Joko Raharjo Tri Redjeki Triyono Triyono Ulma, Zeni Umar Santoso Widodo, Muslih Anwar Winarto Haryadi