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Efek Ekstrak Etanol Biji Kakao (Theobroma cacao) sebagai Antibakteri terhadap Pseudomonas aeruginosa secara In Vitro (The Effect of Ethanolic Extract of Cocoa Beans (Theobroma cacao) as an Antibacterial against Pseudomonas Devi Chintya Kumalasari; Enny Suswati; Misnawi Misnawi
Pustaka Kesehatan Vol 3 No 1 (2015)
Publisher : UPT Percetakan dan Penerbitan Universitas Jember

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Abstract

Nosocomial infection is one of the most serious healthy problem in the world. It causes by Pseudomonas aeruginosa which has a high resistance to antibiotic. One alternative to overcome this problem is the utilization of chocolate (Theobroma cacao) because it contains polifenol that can be used as an antibacterial agent. The aim of this research was to determine antibacterial effect and Minimum Inhibitory Concentration (MIC) of ethanolic extract of cocoa beans to P. aeruginosa by in vitro method. Antibacterial activity test used disk diffusion (Kirby Bauer) method. This type of research was a quasi-experimental with posttest only control group design. Samples used was colony of P. aeruginosa. Concentrations of test solution used were 7,81; 15,62; 31,25; 62,50; 125; 250; 500; and 1000 mg / ml. Positive control used cefepime suspension and negative control used sterile distilled water. The result showed that inhibition zones formed at a concentration of 7,81 mg/ml to 1000 mg/ml. The higher the concentration of the cocoa beans extract, the wider the inhibition zones formed. By direct measurement, MIC was obtained at the dose of 7.81 mg / ml while statistical analysis yielded an MIC value of more than 6.95 mg / ml. In conclusion, the ethanolic extract of cocoa beans had antibacterial effect on P. aeruginosa in vitro.   Keywords: antibacterial effect, cocoa beans, Pseudomonas aeruginosa
Uji in vitro Aktivitas Antibakteri Minyak Atsiri Batang Sereh (Cymbopogon citratus) terhadap Shigella dysenteriae Yunita Wulansari; Enny Suswati; Septa Surya Wahyudi
Pustaka Kesehatan Vol 6 No 2 (2018)
Publisher : UPT Percetakan dan Penerbitan Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/pk.v6i2.7665

Abstract

Shigella dysenteriae is bacteria that caused diarrhea with high morbidity and mortality. Resistance and toxicity has reported in some antibiotics therapy towards S. dysentriae. Therefore, alternative therapy based on herbal plants is needed. Chymbopogon citratus oil can be used as an antibacterial to Escherichia coli, Klebsiella pneumoniae, Streptococcus mutans, all strains Staphlylococcus sp, Bacillus cereus and Bacillus subtilis. This study aimed to investigate antibacterial activity of C. citratus oils on the growth of S. dysentriae and its minimum inhibitory concentration (MIC). This type of research was a quasi experimental. Antibacterial activity test performed by diffusion method. Subjects in this study were S. dysentriae. C. citratus oil concentration used were 15 μl/ml, 20 μl/ml, 25 μl/ml, 30 μl/ml, 35 μl/ml and 40 μl/ml. Positive control group was given ciprofloxacin 5 μl/ml and a negative control group was given tween-80. Inhibition zone was observed at a concentration of 25 µl/ml (P3). The MIC of C. citratus oils againts S. dysentriae was at a concentration of 25 µl/ml. Kruskal Wallis analysis showed that p=0.000 and α=0.05, it was revealed that there were differences between antibacterial activity amoung tested group with different concentration. Keywords: Shigella dysenteriae, Chymbopogon citratus oil, antibacterial
Pengaruh Pemberian Jus Semangka terhadap Kelelahan Otot dan Delayed Onset Muscle Soreness setelah Latihan Beban (The effect of Watermelon Juice on Muscle Fatigue and Delayed Onset Muscle Soreness after Weight Training) Prayoga Adinawer Sirait; Cholis Abrori; Enny Suswati
Pustaka Kesehatan Vol 4 No 1 (2016)
Publisher : UPT Percetakan dan Penerbitan Universitas Jember

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Abstract

The main problem that is often faced by athlete is fatigue. It can decrease maximum muscle strength. To overcome this problem, many athletes use ergogenic aids such as watermelon. The purpose of this study was to determine the effect of watermelon juice on reduction of muscle fatigue and delayed onset muscle soreness (DOMS) after weight training. Muscle fatigue was measured by maximum repetition done by the subjects in the last set and DOMS was measured by post-training muscle soreness intensity. By using a randomized crossover design, 20 subjects were recruited then divided into 2 groups, a treatment group and a control group. The subjects were asked to do a training protocol to induce muscle fatigue and DOMS. As much as 500 ml watermelon juice was consumed in 1 of 2 training sessions and a placebo was consumed in the other. The number of repetitions showed a significant increase on treatment group than control group (p<0,05). Passive muscle soreness intensity and active muscle soreness intensity showed a significant decrease on 24 hours and 48 hours post-training after administration of watermelon juice (p=0,000). We conclude that there was an effect of watermelon juice on muscle fatigue and DOMS after weight training.   Keywords: weight training, muscle fatigue, DOMS, watermelon, citrulline
Aktivitas Ekstrak Metanol Bawang Putih (Allium sativum) sebagai Penghambat Pembentukan Biofilm pada Pseudomonas aeruginosa (The Activity of Methanolic Extract of Garlic (Allium sativum) in Inhibiting Growth of Biofilm in Pseudomonas aeruginosa) Risty Pradana Linggan Wangi; Enny Suswati; Desie Dwi Wisudanti
Pustaka Kesehatan Vol 5 No 3 (2017)
Publisher : UPT Percetakan dan Penerbitan Universitas Jember

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Abstract

Garlic (Allium sativum) is one of the most important agricultural product that has organosulphur compound, such as alliin, allicin, and ajoene. Those organosulphur compound used as antibiofilm agent by inhibiting adhesion and quorum sensing process in Pseudomonas aeruginosa. The purpose of study was to identify the antibiofilm effect and Minimum Biofilm Inhibitory Concentration (MBIC) of methanolic extract of garlic against the growth of P. aeruginosa biofilm. This was quasi experimental with posttest only control group design. The colony of P. aeruginosa were divided into 7 groups with 5 groups used methanolic extract of garlic as P1-P5 at a dose 0,3; 0,6; 0,9;1,2; and 1,5 mg/mL, positive control group (K+) used NAC 80 mg/mL, and negative control group (K-) used sterile water. This research using Microtitter Plate Biofilm Assay Method with 4 replication. The absorbance value was analized by using One Way Anova statistical analysis, it showed the significance value p=0,000 (p<0,05). By using linier regression, the MBIC was obtained at the dose of 0,3468 mg/mL. In conclusion, methanolic extract of garlic had ability to inhibit the growth biofilm of P. aeruginosa. Keywords: biofilm, garlic, Minimum Biofilm Inhibitory Concentration, P. aeruginosa
Respon Imunogenitas Antibodi Poliklonal IgY terhadap Protein Adhesi Pili 95 kDa Shigella dysenteriae asihanti rosita ferdiana; Enny Suswati; Kristianningrum Dian Sofiana
Pustaka Kesehatan Vol 2 No 2 (2014)
Publisher : UPT Percetakan dan Penerbitan Universitas Jember

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Abstract

Shigella dysenteriae is the most frequently cause of deaths of dysentery cases in infants and toddlers. Shigella dysenteriae has a pili that act as an adhesin molecule and will detect in human body as antigens which is involved in producing antibodies. This study was conducted to prove that Shigella dysenteriae pili adhesion protein 95 kDa is immunogenic. This was an experimental laboratory study with quasy experimental design in vitro. The study began with mice erythrocyte cell isolation, IgY polyclonal antibodies isolation, mice intestinal enterocytes cell isolation, hemagglutination inhibition test, and adhesion inhibition test. The haemagglutination inhibition test showed that the pili adhesion protein 95 kDa of S. dysenteriae able to inhibit the haemagglutination of erythrocytes mice Balb/C up to ½ dilution. While, the adhesion inhibition test revealed that the fewer of antibody concentration on enterocytes, the greater bacteria attached. According to the linear regression test results, the index adhesion value increased along with the decreased of antibody concentration. In conclusion, pili adhesion protein 95 kDa from S. dysenteriae was immunogenic and could inhibit the hemagglutination and adhesion between S. dysenteriae and mice enterocytes cells.   Keywords: immunogenicity, IgY polyclonal antibody, Shigella dysenteriae, pili adhesion protein.
Hubungan perilaku hidup bersih dan sehat dengan kejadian infestasi Soil–transmitted Helminths pada pekerja perkebunan kopi Sumber Wadung Kabupaten Jember Ahmad Hasbi Al-Muzaky; Bagus Hermansyah; Enny Suswati; Yunita Armiyanti; Yudha Nurdian
JURNAL KEDOKTERAN DAN KESEHATAN Vol 6, No 1 (2019)
Publisher : Fakultas Kedokteran Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (293.379 KB) | DOI: 10.32539/JKK.v6i1.7233

Abstract

Infestasi Soil - Transmitted Helminths (STH) merupakan masalah kesehatan yang masih sering ditemukan di negara beriklim tropis, salah satunya di Indonesia. Hal ini dikarenakan perkembangan STH membutuhkan kondisi lingkungan yang hangat dan lembab, serta  memerlukan media tanah untuk menjadi fase infektif yang disebut soil - transmitted helminth.Kondisi ini dapat ditemukan di lingkungan perkebunan yang sangat menguntungkan bagi perkembangan STH. Hal ini menyebabkan para pekerja perkebunan sangat beresiko terinfestasi oleh STH. Tujuan penelitian ini adalah mengetahui adanya hubungan perilaku hidup bersih dan sehat (PHBS) dengan kejadian infestasi STH pada pekerja perkebunan kopi. Jenis penelitian ini adalah penelitian analisis deskriptif dengan desain cross-sectional yang dilaksanakan pada bulan Desember 2018 hingga Januari 2019. Perkebunan yang dipilih adalah Perkebunan Kopi Sumber Wadung di Kabupaten Jember dengan jumlah sampel sebanyak 100 orang responden dan diberikan kuisioer PHBS. Pemeriksaan infestasi STH dilakukan dengan dua metode yaitu flotasi dan sedimentasi. Dari 100 responden, 28 pekerja (28%) terinfestasi oleh STH. Kemudian dilakukan uji analisis chi-square antara PHBS dengan kejadian infestasi STH dan didapatkan adanya hubungan antara PHBS dengan kejadian infestasi STH pada pekerja (<0,05).
ADVERSE HEALTH IMPACTS OF PESTICIDE USE ON INDONESIAN RICE PRODUCTION: AN ECONOMIC ANALYSIS ENNY SUSWATI; NUR K. AGUSTIN; JOKO MARIYONO
SOCA: Jurnal Sosial Ekonomi Pertanian Vol. 6, No. 2 Juli 2006
Publisher : Program Studi Agribisnis, Fakultas Pertanian, Universitas Udayana Jalan PB.Sudirman Denpasar, Bali, Indonesia. Telp: (0361) 223544 Email: soca@unud.ac.id

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Abstract

Pestisida bak pisau bermata dua. Di satu sisi, pestisida memberikan manfaat bagi pengguna,dan di sisi lain pada waktu yang bersamaan pestisida mempunyai efek yang merusak. Olehkarena itu pelarangan penggunaan secara total tidak efisien, dan menentukan penggunaanpestisida yang optimal sangatlah diperlukan, dengan cara mempertimbangkan dampakkesehatan. Tujuan kajian ini adalah menentukan penggunaan pestisida yang optimal denganmempertimbangkan dampak kesehatan, dan mengestimasi nilai moneter kehilanganproduktivitas petani. Manfaat penggunaan pestisida dicari dengan fungsi produksi, sedangkandampak kesehatan dicari dengan fungsi biaya kesehatan. Data produksi padi selama kurunwaktu 1974-2000 digunakan untuk mencari fungsi produksi. Fungsi biaya kesehatan diperolehdari kajian sebelumnya yang telah dilakukan oleh peneliti pendahulu. Hasil kajian inimenunjukkan bahwa penggunaan pestisida yang optimal sangat rendah dan nilai ekonomikehilangan produktivitas sangat tinggi. Hal ini karena adanya perbedaan yang besar antarapenggunaan pestisida yang optimal dengan penggunaan pestisida yang aktual selama kurunwaktu tersebut. Faktor penting yang menyebabkan besarnya perbedaan tersebut adalahelastisitas produksi dari pestisida sangat rendah. Saran yang dapat disampaikan adalah petanimenggunakan pestisida secara efisient, yaitu mendekati tingkat penggunaan yang optimal.
KETIMPANGAN JENDER DALAM AKSES PELAYANAN KESEHATAN RUMAH TANGGA PETANI PEDESAAN: KASUS DUA DESA DI KABUPATEN TEGAL, JAWA TENGAH JOKO MARIYONO; APRI KUNTARININGSIH; ENNY SUSWATI
SOCA: Jurnal Sosial Ekonomi Pertanian Vol. 8, No. 2 Juli 2008
Publisher : Program Studi Agribisnis, Fakultas Pertanian, Universitas Udayana Jalan PB.Sudirman Denpasar, Bali, Indonesia. Telp: (0361) 223544 Email: soca@unud.ac.id

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Abstract

The role of woman in rural and agricultural development is very important because more thana half of work in agriculture and rural areas is carried out by woman labors. Consequently, thewomen ought to have equity in right with men to get health services. But, there is a stronghypothesis that women have less access to health services than men because of social andcultural factors in rural areas. This study aims to examine the gender disparity anddiscrimination of health. The disparity is measured using concentration curve andconcentration index, whereas the discrimination is approached using microeconomic theory ofconsumption. The results of indicate that there is a small difference in health disparitybetween women and men; even women get more portion than men. This is because womenhave specific characteristics in terms of health problem, in which men do not have.
The Relationship of Delayed Appendectomy to the Incidence of Surgical Site Infection (SSI) in Acute Appendicitis Patients: A Narrative Review Wahyuning Ati Ashari; Enny Suswati; Erfan Efendi
Media Penelitian dan Pengembangan Kesehatan Vol 31 No 3 (2021)
Publisher : Sekretariat Badan Penelitian dan Pengembangan Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22435/mpk.v31i3.4775

Abstract

Acute appendicitis is one of the most common causes of surgery worldwide. Appendectomy is a skeptical surgical intervention associated with the risk of Surgical Site Infection (SSI). The purpose of this analysis was to describe the relationship between delayed appendectomy in acute appendicitis and the incidence of SSI. Reviewers selected articles containing data on the incidence of SSI due to appendectomy delays for 3 to 48 hours from seven databases, namely Pubmed, Nature, SpringerLink, Science Direct, ProQuest, Oxford Open Access Journal, and Cochrane Library. The data source was secondary data from international articles published in 2011 to 2020 based on the PICO criteria. The data obtained were then grouped and synthesized without meta-analysis/Synthesis Without Metaanalysis (SWiM) descriptively. A total of 2,778 articles were collected, of which 24 studies met the inclusion criteria. A total of 4 articles showed an association between appendectomy delay and SSI while the other 20 articles did not show a relationship between the two. There was no relationship between appendectomy delays for less than 48 hours since hospital admission to surgery with the incidence of SSI, however a delayed for more than 48 hours showed a significant value. The results showed there was no relationship between delayed appendectomy for less than 48 hours since the time the patientwas admitted to the hospital until the surgery took place, but a delayed appendectomy delay for more than 48 hours showed a significant relationship with the incidence of SSI. This narrative review supports early surgical intervention of acute appendicitis cases by considering the severity of the patient to avoid other surgical complications. Abstrak Apendisitis akut menjadi salah satu penyebab pembedahan yang paling sering ditemukan di seluruh dunia. Apendektomi merupakan intervensi bedah skeptis yang berhubungan dengan risiko terjadinya Surgical Site Infection (SSI). Tujuan analisis ini untuk mendapatkan gambaran hubungan antara penundaan apendektomi pada apendisitis akut dengan kejadian SSI. Reviewer memilih artikel yang memuat data kejadian SSI akibat penundaan apendektomi selama 3 sampai 48 jam dari tujuh basis data yaitu Pubmed, Nature, SpringerLink, Science Direct, ProQuest, Oxford Open Access Journal, dan Cochrane Library. Sumber data berupa data sekunder dari artikel internasional yang diterbitkan pada tahun 2011 hingga 2020 berdasarkan kriteria PICO. Data yang didapatkan kemudian dikelompokkan dan disintesis tanpa meta-analisis/Synthesis Without Meta-analysis (SWiM) secara deskriptif. Total artikel yang dikumpulkan adalah 2.778 artikel, sebanyak 24 studi memenuhi kriteria inklusi. Sebanyak 4 artikel menunjukkan hubungan antara keterlambatan apendektomi dengan SSI sedangkan 20 artikel lainnya tidak menunjukkan hubungan keduanya.Tidak ada hubungan penundaan apendektomi kurang dari 48 jam sejak masuk rumah sakit hingga operasi dengan insidensi SSI namun penundaan lebih dari 48 jam menunjukkan nilai yang signifikan. Berdasarkan hasil analisis, disimpulkan bahwa tidak terdapat hubungan penundaan apendektomi selama kurang dari 48 jam sejak pasien masuk rumah sakit hingga proses pembedahan berlangsung tetapi penundaan apendektomi lebih dari 48 jam menunjukkan adanya hubungan signifikan terhadap kejadian SSI. Tinjauan naratif ini mendukung intervensi bedah yang cepat pada kasus apendisitis akut dengan mempertimbangkan tingkat keparahan pasien dan untuk menghindari komplikasi pembedahan lain.
EVALUASI KEAMANAN SUMBER AIR MINUM DESA MOJO KECAMATAN PADANG KABUPATEN LUMAJANG Aditya Oktavianto; Nurhayati Nurhayati; Enny Suswati
JURNAL AGROTEKNOLOGI Vol 8 No 02 (2014)
Publisher : Faculty of Agricultural Technology, University of Jember

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Abstract

Little known about the safety of natural water on enteric mikroflora population at Mojo Village Padang Distric Lumajang Regency. We assessed the population of enteric microflora i.e Salmonella and Eschericia coli in the wellspring. We performed a randomized sampling to evaluate the population of Salmonella and Eschericia coli of three natural water resource at Mojo Village. There were Jirun, Kali Tengah and Sumber Suko water resource. Medium to colony identification were used Salmonella Chromogenic Agar (SCA) and Enteric Hektoin Agar (HEA). SCA resulted blue colony for Eschericia coli and magentha or violet colony for Salmonella. While HEA resulted orange colony for Eschericia coli and green for Salmonella. 250 mL of natural water was used to growth the enteric microflora. We observed three water resource of Mojo Village contain enteric microflora. The enteric microflora population was up to 2 log10 CFU/mL (more than 10 CFU/mL). These was a fantastic population, because enteric population must no growth on drink water. Assuming the enteric microflora come from fish faecal, because at the surrounding of water resource growth the fish. Three water resource at Mojo Village were no safety to used as drink water if without water treatment. The enteric microflora can dextructed by using filtration and UV treatment or thermal process example water boiling.Keywords: Jirun, Kali Tengah, Sumber Suko, Salmonella, Eschericia coli, water resource
Co-Authors Abu Khoiri Aditya Oktavianto Ahmad Hasbi Al-Muzaky Ahmad Hasbi Al-Muzaky Alfianti, Nita Angga Mardro Raharjo Anggraini, Nanda Dewi Anisa Rizca Putri APRI KUNTARININGSIH Arifah Nur Hasanah Ariska Nur Aida asihanti rosita ferdiana Azham Purwandhono Bagus Hermansyah Bagus Hermansyah Bagus Satrio Pambudi Bagus Satrio Pambudi, Bagus Satrio Cholis Abrori, Cholis Desie D. Wisudanti Desie Dwi Wisudanti Devi Chintya Kumalasari Dian H. Purnamasari Diana C Diana C. Mufida Diana Chusna Mufida Dini Agustina Dwita Aryadina Dwita Aryadina Rachmawati Elly Nurus Sakinah, Elly Nurus Elvia Rahmi Marga Putri Erfan Efendi Habib Mustofa Haq, Hanifah S Ika Rahmawati Sutejo Indra Samudra Rahmat Jauhar Firdaus Joko Mariyono Kirana Nadyatara Kristianningrum Dian Sofiana Lailatis Shofia Laksmi Indreswari Lutfadaturroifa, Alya Wafaul M. Ali Shodikin Misnawi Misnawi Misnawi Misnawi Mufida Mufida Mufida, Diana C Muhammad A. Shodikin Muhammad Alif Taryafi Mukarromah, Laylatul Fitriyah Nadhiva, Alvien Zahrotun Nexia Nevarachell Onny Amirsyah Nisrina Salsabila Firmansyah Nita Alfianti Nugraha, Bima Setia Sandya NUR K. AGUSTIN Nurhayati Nurhayati Pratama, Dava Rizky Prayoga Adinawer Sirait Probosutiksna, Nuno Febrian Probosutiksna, Nuno Febrian Putra Uta, Diego Rafi Putu Ayu Laksmi Lestari Raharjo, Angga M Rahmat, Indra Samudra Regina Finka Risty Pradana Linggan Wangi Rumastika, Nindya Shinta S.Pd. M Kes I Ketut Sudiana . Sakinah, Elly N Salsabila, Yuna Annisa Saskia Mediawati septa surya wahyudi, septa surya Shodikin, M. Ali Shofia, Lailatis Sudarmanto, Yohanes Supangat Supangat Supangat Supangat Suryono Suryono Syahputri, Siti Ananda Hardita Ulfa Elfiah Wahyuning Ati Ashari Wiwien Sugih Utami Wiwin Sugih Utami Yudha Nurdian Yudha Nurdian Yuna Annisa Salsabila Yunita Armiyanti Yunita Wulansari