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All Journal Natural Science: Journal of Science and Technology Biocelebes AGROLAND: The Agricultural Sciences Journal
I Nengah Suwastika
Tadulako University
Agriculture, Biological Sciences & Forestry Computer Science & IT

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INDUKSI KALUS KLON KAKAO (THEOBROMA CACAO L) SULAWESI 2 PADA MEDIUM MS DENGAN PENAMBAHAN 2,4-D, BAP DAN AIR KELAPA Urfiana, Urfiana; Haliana, Haliana; Muslimin, Muslimin; Suwastika, I Nengah
Natural Science: Journal of Science and Technology Vol 2, No 1 (2013): Volume 2 Number 1 (March 2013)
Publisher : Univ. Tadulako

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Abstract

Sulawesi 2 cacao clone is one of the clones which grown by local farmer in Central Sulawesi. One problem in cacao development in this time is the availability of high quality of seeds. One posible way in overcoming of this problem is through the application of tissue culture techniques via embryogenesis. The early stages of embryogenesis is callus induction, which aim to get emryonic callus cells and it can be a model in cacao research. This study was conducted over March and June 2013 in the Tissue Culture Laboratory, Faculty of Forestry Tadulako University. Explant that used was Stamen of flower of Sulawesi 2 cacao clone. This experiment was based on a complete randomized design (CRD) with 4 treatments, and it were repeated 3 times. On every single unit was growth 10 of explants. Different medium as treatments tested were: MS0 + 2 ppm 2,4-D + (15%) Coconut water (MK1), MS0 + 2 ppm 2,4-D + 0.2 ppm BAP + (15%) Coconut water (MK2) , MS0 + 3 ppm 2,4-D + (15%) coconut water (MK3), ms0 + 3 ppm 2,4-D + 0.2 ppm BAP + (15%) coconut water (MK4). Callus development were observed based on the emergence of callus, the percentage of explants producing callus, and cell callus morphology. Callus induction was done under dark condition and  temperature of 26º C. The results showed that all of the tested treatments can induce the callus of cacao. The best medium was MS medium + 3 ppm 2,4-D + (15%) Coconut water (MK3), characterized by the appearance of white, callus in intermediate-type, the callus mass was relatively larger than its under others treatments. Under this treatment, explant can produce uniform-relatively big cell and active in proliferation in 10 days after culture
CHARACTERIZATION OF α-AMYLASE ACTIVITY FROM THERMOPHILIC BACTERIA ISOLATED FROM BORA HOT SPRING, CENTRAL SULAWESI Gazali, Faris Muhammad; Ananda, Meryany; Suwastika, I Nengah
Natural Science: Journal of Science and Technology Vol 7, No 1 (2018): Volume 7 Number 1 (March 2018)
Publisher : Univ. Tadulako

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Abstract

?-Amilase (EC 3.2.1.1) merupakan salah satu jenis enzim yang sangat penting dalam bioteknologi. Enzim ini sering diaplikasikan pada berbagai sektor industri, seperti industri deterjen, tekstil, kertas, roti, alkohol, dan pati. Termostabilitas dari ?-Amilase merupakan sifat utama dari enzim yang dibutuhkan pada industri pemerosesan pati. ?-Amilase dengan sifat termostabil dapat diperoleh dari bakteri termofilik yang secara alami dapat diisolasi dari mata air panas. Mata air panas Bora merupakan salah satu habitat yang paling berpotensi dalam mengisolasi bakteri termofilik karena memiliki suhu air yang sangat tinggi hingga mencapai 90,1°C. Penelitian ini bertujuan untuk mengetahui karakter aktivitas ?-Amilase dari bakteri termofilik lokal isolat mata air Bora, Sulawesi Tengah. Terdapat 13 (dari 18 isolat) bakteri termofilik isolat mata air panas Bora yang mampu menghasilkan ?-Amilase yang berhasil diisolasi dari sampel sedimen. Dari 13 isolat tersebut, 3 isolat terpilih dideskripsikan pada tulisan ini, yakni BR 001, BR 006, and BR 012. Ketiga isolat tersebut memiliki karakter: nilai indeks amilolitik berkisar 0,78 ? 1,44; aktivitas ?-Amilase berkisar berkisar 3,67 U/ml ? 7,6 U/ml; konsentrasi protein berkisar 0,02 mg/ml ? 0,14 mg/ml; aktivitas spesifik enzim kasar ?-Amilase berkisar 54,83 U/mg ? 308,45 U/mg; ?-Amilase yang aktif pada kisaran suhu 32°C ? 80°C; dan ?-Amilase yang aktif pada kisaran pH 5 ? 7 dan memiliki pH optimum pada kisaran 6,5 ? 7. Adanya aktivitas enzim pada suhu yang tinggi dari ?-Amilase yang diproduksi oleh isolat bakteri termofilik lokal dari mata air panas Bora menunjukkan adanya potensi dalam aplikasi enzim lokal pada sektor industri di masa depan, khususnya pada industri pemerosesan pati.
ORGANOGENESIS TANAMAN BAWANG MERAH (ALLIUM ASCALONICUM.L) LOKAL NAPU SECARA IN VITRO PADA MEDIUM MS DENGAN PENAMBAHAN IAA DAN BAP Utami, Felma Tri; Haliani, Haliani; Muslimin, Muslimin; Suwastika, I Nengah
Natural Science: Journal of Science and Technology Vol 2, No 2 (2013): Volume 2 Number 2 (August 2013)
Publisher : Univ. Tadulako

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Abstract

The aims of this study were to find out the optimum combination of IAA and BAP hormones in MS based medium, which suitable for promoting in vitro-organogenesis. In vitro experiment on inducing organogenesis of Shallot c.v. Local Napu showed that combination of 0.01 ppm IAA and 1 ppm BAP, it was the best combination on MS based medium for that organogenesis. These result was based on the measurements over several parameters, including: period of emerging shot and root, precentage of explant growth, number of roots, number of leaves, number of stomata, also chlorophil content of planlet leaves. This data suggesting that MS medium containing 0.01 ppm IAA and 1 ppm BAP was a potential medium to be used on propagation of shallot c.v. Local Napu.
KARAKTERISASI SELULASE ASAL BAKTERI TANAH DANAU KALIMPA’A SULAWESI TENGAH Marina, Marina; Lambui, Orryani; Suwastika, I Nengah
Natural Science: Journal of Science and Technology Vol 7, No 2 (2018): Volume 7 Number 2 (August 2018)
Publisher : Univ. Tadulako

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Abstract

Selulase adalah enzim yang dapat dihasilkan oleh bakteri selulolitik, memiliki kemampuan dalam menguraikan selulosa menjadi monomer yang lebih sederhana di alam. Danau Kalimpa?a yang merupakan kawasan Taman Nasional Lore Lindu Sulawesi Tengah memiliki produktivitas seresah yang tinggi, sehingga sangat bergantung pada keberadaan mikroorganisme tanah termasuk bakteri selulolitik sebagai dekomposer. Pada penelitian ini telah dilakukan isolasi dan karakterisasi enzim endogenous. Metode yang digunakan dalam penelitian ini adalah deskriptif kualitatif dan deskripsi aktivitas enzim dipaparkan secara kuantitatif. Karakterisasi selulase ditentukan dengan menguji aktivitas enzim pada variasi suhu (30°C, 40°C, dan 50°C) dan variasi pH (5.0; 6.0; 7.0; dan 8.0). Hasil isolasi bakteri diperoleh 5 isolat terbaik (K1, K2, K3, K4 dan K5) yang memiliki kemampuan dalam mendegradasi selulosa. Secara kualitatif, isolat K4 dapat mendegradasi selulosa lebih cepat dibanding isolat lain berdasarkan pembentukan zona bening dengan Indeks Aktivitas Selulase (IAS) sebesar 3,88 mm. Secara kuantitatif, aktivitas selulase ditentukan berdasarkan kadar gula reduksi yang dihasilkan menggunakan metode DNS (Di Nitro Salisilic Acid). Isolat K2 mampu menghasilkan enzim dengan aktivitas selulase tertinggi yaitu 0,30 U/ml dan aktivitas selulase terendah dihasilkan oleh isolat K1 yaitu 0,001 U/ml. Suhu dan pH optimum selulase adalah 40°C dan pH 6, yang menghasilkan aktivitas enzim sebesr 0,279 U/ml.
UJI AKTIVITAS SELULASE DARI JAMUR SELULOLITIK ASAL TANAH DANAU KALIMPA’A SULAWESI TENGAH Talantan, Vrillia Marannu; Marina, Marina; Lambui, Orryani; Suwastika, I Nengah
Natural Science: Journal of Science and Technology Vol 7, No 3 (2018): Volume 7 Number 3 (December 2018)
Publisher : Univ. Tadulako

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Abstract

Selulase (E.C.3.2.1) merupakan enzim yang berperan dalam menguraikan polimer kompleks selulosa menjadi monomer sederhana glukosa. Enzim ini dapat dihasilkan oleh jamur selulolitik. Tanah sekitar Danau Kalimpa?a memiliki kandungan selulosa yang cukup tinggi sehingga keberadaan jamur selulolitik berperan sebagai pengurai. Tahapan isolasi berhasil mendapatkan 13 isolat jamur. Uji kualitatif berdasarkan aktivitas selulase melalui metode pewarnaan congored 0,1% pada media CMC menunjukkan bahwa 7 isolat berpotensi menghasilkan selulase yaitu isolat JIK3, JIK4, JIK5, JIK6, JIK9, JIK10 dan JIK13 yang ditandai dengan terbentuknya zona bening disekitar koloni dengan indeks aktivitas selulase tertinggi dihasilkan oleh isolat JIK13 yaitu 0,57 mm. Uji Kuantitatif aktivitas selulase ditentukan dengan menghitung kadar gula reduksi melalui metode DNS (3,5-Dinitro Salisilic Acid) menunjukkan bahwa isolat JIK13 mampu menghasilkan aktivitas enzim selulase tertinggi yaitu 80,925 U/mL. Berdasarkan identifikasi baik secara makroskopis dan mikroskopis isolat yang berpotensi menghasilkan selulase merupakan anggota genus Aspergillus
ORGANOGENESIS TANAMAN JERUK KEPROK (CITRUS NOBILIS LOUR.) SECARA IN VITRO PADA MEDIA MS DENGAN PENAMBAHANBERBAGAI KONSENTRASI IAA (INDOLE ACETID ACID) DAN BAP (BENZYL AMINO PURIN) Harliana, Harliana; Weaniati, Weaniati; Muslimin, Muslimin; Suwastika, I Nengah
Natural Science: Journal of Science and Technology Vol 1, No 1 (2012)
Publisher : Univ. Tadulako

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Abstract

Research on"Organogenesis of Tangerine orange (Citrus nobilisLour) onMSmediumsupplemented withvariousconcentrations ofIAAandBAP," has been performedatthe Tissue CultureLaboratory ofthe Faculty ofForestry, Tadulako University, Palu during the period of January andMay 2012. The goal of this study was to determine the optimum growth factor combination of IAA and BAP in MS based medium, for organogenesis of the plant. This research was arranged on Completely RandomizedDesign(CRD) withsix treatments and three replications. Growth factor combinations tested in this study were:  0.1ppm IAA + 0.4ppmBAP(C1), 0.1ppm IAA + 0.6ppm BAP(C2), 0.1ppmIAA+0.8ppmBAP(C3), 0.1ppm IAA + 1.0ppmBAP(C4), 1.0ppm IAA + 0.6ppmBAP(C5), and1.0ppmIAA+1.0ppmBAP(C6). The result indicating that the best organogenesis of Orange explant was in MS medium supplemented with 0.1 ppm IAA and 1.0 ppm BAP (C4). This medium was suitable in inducing shoot and leaf, faster than its in other medium. Explant on this medium was also produced higher number of leaves than it on other treatments.
UJI EFEKTIVITAS EKSTRAK DAUN KIRINYUH (CHROMOLAENA ODORATA (L.) R. M. KING DAN H. ROB) SEBAGAI HERBISIDA ALAMI TERHADAP PERKECAMBAHAN BIJI KACANG HIJAU (VIGNA RADIATA (L.) R.WILCZEK) DAN BIJI KARUILEI (MIMOSA INVISA MART. EX COLLA) Frastika, Dian; Pitopang, Ramadhanil; Suwastika, I Nengah
Natural Science: Journal of Science and Technology Vol 6, No 3 (2017): Volume 6 Number 3 (December 2017)
Publisher : Univ. Tadulako

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Abstract

Penelitian uji efektivitas daun kirinyuh (chromolaena odorata (L.) R. M. King & H.Rob) sebagai herbisida alami terhadap perkecambahan biji kacang hijau (Vigna radiata (L.) R. Wilczek) dan biji karuilei (Mimosa invisa Mart. Ex Colla) dilaksanakan pada bulan Juli sampai September 2016, di Laboratorium Bioteknologi Jurusan Biologi Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Tadulako. Penelitian ini disusun berdasarkan model Rancangan Acak Lengkap (RAL) terdiri dari 6 perlakuan dan 3 kali pengulangan dengan konsentrasi ekstrak P0 = 0% P1 = 15% P2 = 20% P3 = 25% P4 = 30% P5 = 35%. Variabel pengamatan meliputi daya kecambah (persentase perkecambahan), laju perkecambahan, panjang hipokotil dan berat basah. Hasil penelitian ini menunjukan bahwa pemberian ekstrak daun kirinyuh Chromolaena odorata berpengaruh dalam menghambat perkecambahan biji kacang hijau Vigna radiata dan biji karuilei Mimosa invisa. Pengambatan perkecambahan biji karuilei M. invisa dan kacang hijau V. radiata dimulai dari konsentrasi 15% sampai 35%. Hasil skrining fitokimia daun C. Odorata positif mengandung senyawa metabolit sekunder, yaitu saponin, tanin, flafonoid, alkaloid dan fenilok.
INDUKSI KALUS TANAMAN KAKAO (Theobroma cacao L.) KLON SULAWESI 1 (S1) PADA MEDIUM MS DENGAN KOMBINASI HORMON 2,4-D, BAP DAN AIR KELAPA Wilma Wilma; Muslimin Muslimin; I Nengah Suwastika
Biocelebes Vol. 8 No. 1 (2014)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

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Abstract

Kakao's seed that can result same plant better with it superior parent indispensable. One of alternative is with utilize vegetative organ origin seed that resulting through network culture tech by processes embriogenesis somatik. This research constitute startup phase in embriogenesis which is plant callus induction kakao. Research was performed on month of March until August 2013 at Network culture Laboratory, kehutanan's faculty. Tadulako's university. Eksplan who is utilized as staminodia of kakao's flower part Sulawesi clone 1. attempt it is arranged bases fledged Random Design (RAL) with 4 conducts and 3 time replicate. Each one unit experimentaling to utilize 10 eksplan. Conduct that dicobakan which is MS0 + 1ppm 2,4 - D. + 0,1ppm BAP + Coconut Water 15% (KW 1 ), MS0 + 1 ppm 2,4 - D. + 0,2ppm BAP + Coconut Water 15% (KW 2 ), MS0 + 2 ppm 2,4 - D. + 0,1ppm BAP + Coconut Water 15% (KW 3 ) and MS0'S medium + 2 ppm 2,4 - D. + 0,2ppm BAP + Coconut Water 15% (KW 4 ). Observed parameter is while its appearance callus, eksplan's percentage that results callus, and callus morphology. Preserve is done at dark place on temperature 26 ºC. Result observationaling to point out all conduct that dicobakan that menginduksi can kakao's callus. MS0'S medium + 2 ppm 2,4 - D. + 0,1ppm BAP + Coconut Water 15% (KW 3 ) constituting best media for menginduksi callus that marked by its appearance chromatic callus turns white, get intermediet's type, greater relative callus mass, uniform and active clefts to begin 14 days culture afterses. Keywords: MS, 2,4 - D., BAP, Coconut water, Callus induction, Staminodia, Theobroma cacao L.
KAJIAN ETNOBOTANI TUMBUHAN PANGAN PADA MASYARAKAT SUKU SEKO DI DESA TANAH HARAPAN KECAMATAN PALOLO KABUPATEN SIGI SULAWESI TENGAH Bill Yorsan Yonathan; I Nengah Suwastika; Ramadhanil Pitopang
Biocelebes Vol. 10 No. 1 (2016)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

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A research entitled “Ethnobotanical Study of food Plant in the Seko ethnic group in Tanah Harapan Village, Sigi Regency, District Central Sulawesi” has been carried out from March to April 2015. The research objective was to obtain the information of Plant diversity, part of the plant used as food and how to use as food. The research was done by methods that is using interview technique to 40 respondents with quisioner sheet. The result showed that there were fifty five (55) plants species that used by the Seko ethnic group in Tanah Harapan Village. The highest percentage that used in the part of plants were 40% of fruit. The Seko ethnic group in Tanah Harapan village utilize plants as food in a way that it can be consumed directly or to go through the first processing is cooked, boiled, fried, baked and coconut milk added.   Key word : Seko Ethnic, Tanah Harapan Village, Sigi Central Sulawesi, Ethnobotanical Food Plant.
GAMBARAN INDEKS MASSA TUBUH PENDERITA TUBERKULOSIS (TB) POSITIF YANG MELAKUKAN PENGOBATAN DI GERDUNAS TB PARU RUMAH SAKIT UNDATA PALU SULAWESI TENGAH Fadhalna Fadhalna; Ihwan Ihwan; I Nengah Suwastika
Biocelebes Vol. 11 No. 1 (2017)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

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Research on the description of the patient's BMI (body mass index) of tuberculosis (TB) in the positive treatment of pulmonary tuberculosis at the unit of integrated movement prevention of tuberculosis Undata Hospital Palu has been implemented since April until September 2015. This research aimed to look at the dynamics of BMI of patients during treatment. The method used was the retrospective design patients who present within 2014, which qualified as research sample. There are 45 respondents of eligible subjects complete data up to the end of treatment. The results showed a changeof each category of BMI from the early phase to the final phase of treatment. In theearly phase of treatment, is very thin and thin BMI were 30 respondents fromcategory 1 and category 2, its hows that most patients with early diagnosis of tuberculosis have a BMI below normal. While in the early phase until the end of treatment showed a significant frequency changes in norma lBMI reaches 24.5% categorized 1 and 0.1% incategory 2. This is due to irregular treatment undergone by the patient from the early phase to the final phase of treatment. This indicates that the category 1 more treatment success which is marked by the increasing quality of BMI’s Patients return to normal. While in Category 2 does not show the amount of success, because with drawing treatment.
Co-Authors Agung, Muhammad Budi Akhmad Khumaidi Ali Djamhuri Anis Nurwidayati Anna Rufaida Aras, Mohammad Rezha Ari Sulistiami Asri Pirade Paserang Bill Yorsan Yonathan Cindy Claudia, Cindy Dana Sulistia Eny Yuniati Fadhalna Fadhalna Fahri Fahri Fahri Fahri Felma Tri Utami Frastika, Dian Gazali, Faris Muhammad Haliana Haliana Haliani Haliani Handayani, Mutia Harliana Harliana Haryati Baktiar Herlina Herlina Ihwan Ihwan Imelda, Silvia Jayawarsa, A.A. Ketut Jintan Jintan Laksono Trisnantoro Lina Mahardiana Marina Marina Mazidaturohmah, Mazidaturohmah Melisa S.M. Sorentina Meryany Ananda Mohammad Fahmi Musdalifah Musdalifah Muslimin Muslimin Ni Nyoman Lidyawati Niluh Made Dwi PYD Ningsi, Putri Suria Novika Nurul Aisyah Nurvita Nurvita Orryani Lambui Paserang, Asri P. Pranata, Adhi Rahmansyah Rahmansyah Rahmat Rahmat Ramadanil Pitopang Ramadhanil Pitopang Ramadhanil Pitopang Ramadhanil pitopang Rifka Rifka Riska Tajuddin Rosidah, Maya Sofiyanti Sahril M Taher Sari, Riska Sri Niken Ariati Sri Utami Talantan, Vrillia Marannu Umrah Umrah Urfiana Urfiana Waeniati Waeniati Waeniaty Waeniaty Weaniati Weaniati Wilma Wilma Yundari, Yundari Yunus, Fahrunnisa Yuzammi Yuzammi