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All Journal Natural Science: Journal of Science and Technology Biocelebes AGROLAND: The Agricultural Sciences Journal
I Nengah Suwastika
Tadulako University
Agriculture, Biological Sciences & Forestry Computer Science & IT

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INDUKSI KALUS JARAK PAGAR (Jatropha curcas L.) PADA MEDIA MS DENGAN PENAMBAHAN BERBAGAI KONSENTRASI BAP (Benzyl Amino Purin) DAN 2,4-D (Dichlorophenoxy Acetic Acid) Haryati Baktiar; Muslimin Muslimin; I Nengah Suwastika
Biocelebes Vol. 11 No. 1 (2017)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

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Abstract

This research was conducted from January to June 2015 at the Tissue Culture Laboratory, Faculty of Forestry, Tadulako University. The explant used in this study was the stem of seedling. Experimental was based on a completely randomized design (CRD) with 4 treatments, and 3 replication, with 3 explants on each unit. Growth Medias as treatments were  H1 = MS0 + 0.5ppm BAP + 1.5 ppm 2.4-D; H2 = MS0 +0.5 ppm BAP + 2 ppm 2.4-D, H3 = MS0 + 0.5 ppm BAP + 2.5ppm 2.4-D, andH4 = MS0 + 0.5 ppm BAP + 3ppm 2.4-D. Parameters observation were time in callus appear, type of callus,  color of callus, the weight of callus, morphology of callus cells. Callus was induched on those medium under dark condition at  26-280 C. The result of the research showed that all treatments can induced the callus. The Medium of  ms0 + 0,5ppm BAP + 1.5 ppm of 2,4-D (H1) was the best media for callus induction, as it showed  in short period of  callus induction, the appearance of yellowish-white colors, highest in mass and volume of the callus cell, and showed uniform and active cell in proliferation.
UJIVIABILTAS INOKULUM JAMUR TIRAM PUTIH ( PLEUROTUS OSTREATUS ( JACQ) P. KUMM) DALAM BENTUK SEDIAAN CAIR Siti Fatmawati; Umrah Umrah; I Nengah Suwastika
Biocelebes Vol. 11 No. 1 (2017)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

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Abstract

The research aimed to know the best formulations of basic substrate inoculums  white oyster mushrooms (Pleurotus ostreatus (Jacq) P. Kumm) in the best liquid forms and their viability on production media. The research was conducted at Laboratory  Biotekchnology of Depertement of Biology Faculty of Matematics and Natural Sciences, starting from March until July 2015. The experimental designed used was a Completely Randomized Design (CRD) which consisted of seven treatment and three replications. The compositions of the treatment were the ratio among wood sawdust, rice brain, corn starch and calcium (g) in successive sequences as follows : P0 (0 : 50: 50 : 3); (P1 10 : 45 : 45 : 3); (P2 20 : 40 : 40 : 3); (P3 30 : 35 : 35 : 3); (P4 40 : 30 : 30 : 3); (P5 50 : 25 : 25 : 3); (P6 60 : 20 : 20 : 3). The planting media used  in this research were production media. The observation parameters namely the ability fungal  growth  of inoculums  that meets surface the media with viability a high. The result of the research showed that P6 was the best treatment among. In the viability of the inoculums grew well on media production surface with good viability at 8 days of incubation. 
KEANEKARAGAMAN KUMBANG CERAMBYCID (Coleoptera: Cerambycidae) PADA PERKEBUNAN KAKAO DI TIGA TIPE HABITAT rahmat rahmat; I Nengah suwastika; fahri fahri
Biocelebes Vol. 13 No. 3 (2019)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (220.56 KB) | DOI: 10.22487/bioceb.v13i3.10509

Abstract

Penelitian ini bertujuan untuk mengetahui keanekaragaman kumbang Cerambycid (Coleoptera: Cerambycidae) pada perkebunan kakao di tiga tipe habitat. Penelitian telah dilakukan mulai bulan Juni 2017 hingga April 2018. Koleksi sampel dilakukan menggunakan Atrocarpus trap pada 3 tipe habitat perkebunan kakao yaitu habitat kakao pinggiran hutan (KPH), habitat kakao yang tidak terawat (KTT) dan habitat kakao terawat (KT). Total diperoleh sebanyak 381 individu, yang tergolong dalam 5 tribe, 7 genus dan 13 spesies. Nilai indeks keanekaragaman (H') dan kemerataan (E) tertinggi pada habitat kebun kakao tidak terawat (KTT). Indeks kesamaan Bray-Curtis (IBC), menunjukkan kesamaan antara kebun kakao di pinggiran hutan (KPH) dengan kebun kakao terawat (KT). Hasil dari penelitian ini menunjukkan bahwa tiga jenis perkebunan kakao pada penelitian ini, sangat mendukung keberadaan kumbang cerambycid dan tidak memiliki pengaruh yang signifikan terhadap kehidupan mereka.
INVENTARISASI JENIS DAN TINGKAT KESEHATAN POHON PELINDUNG PADA BEBERAPA TAMAN KOTA PALU SULAWESI TENGAH Sahril M Taher; I Nengah Suwastika; Ramadhanil Pitopang
Biocelebes Vol. 12 No. 3 (2018)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

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Abstract

Research entitled” Inventory species and levels of demage Shade Tree at some Park in Palu Town, Central Sulawesi”, has been conducted from July to August 2015. The research was aimed to inventory of all shade tree species and to determine the levels of damage in four (4) Parks in Palu Town. The data analysis was done on the percentage of wound compact to the diameter of a main trunk and the percentage of the damaged on the trunk the result of the research showed that, there were six (6) species of shade tree belong to five (5) families with individual number seventy four (74) of Trembesi (Samanea saman (Jack.) Merr.), Thirty five (35) of Mahoni (Swetenia mahagoni (L.) Jacq), Twenty eigh (28) of Katapang (Terminalia catappa Linn), six (6) of Banyam (Ficus benjamina Linn), Four (4) of Johar (Senna seamea Lamk), Two (2) of Kayu Jawa (Lannea coromandelica (Houtt.) Merr). The highest percentage of damage on these four (4) ports was found on of Trembesi (Samanea saman (Jacq.) Merr), which grows in GOR City Park. The average of wounded as 2.71 % and the percentage of damage on stems was 20 %.
UJI BEBERAPA FORMULA INOKULUM TERHADAP PRODUKSI TUBUH BUAH JAMUR TIRAM PUTIH (Pleurotus ostreatus (Jacq.) P.Kummer.) Dana Sulistia; Umrah Umrah; I Nengah Suwastika
Biocelebes Vol. 14 No. 1 (2020)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22487/bioceb.v14i1.15086

Abstract

The study of test for several inoculum formulas on the production of white oyster mushroom body (Pleurotus ostreatus (Jacq.) P.Kummer.) conducted from April to September 2018 at Biotechnology laboratory, Department of Biology, Faculty of Mathematics and Natural Sciences, Tadulako University. This study were aimed to determine whether the treatment of the inoculum formula tested on the production medium can form the white oyster mushroom fruit body and to find out which inoculum formula can form the optimum fruit body in the production medium. This study was conducted by Randomized Complete Design (RAL) consisted of 4 treatments and four replications, I1 (100% corn seeds), I2 (50% : 23%: 27%), I3 (50% : 17% : 33%) dan I4 (95% : 5%). The results showed that I 3 was the best inoculum for the growth of the white oyster mushroom fruit body compared to the other inoculums which were characterized by a wide mushroom hood, the length was long and the weight of fresh mushroom was high. The inoculum formula and the best dosage for body production of white oyster mushroom are found in treatment I3 (50% sawdust + 17% corn flour + 33% bran).
FORMULASI MEDIA PRODUKSI JAMUR TIRAM (Pleurotus ostreatus) DENGAN SUPLEMENTASI AMPAS SAGU Sri Utami; Umrah umrah; I Nengah Suwastika
Biocelebes Vol. 14 No. 1 (2020)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22487/bioceb.v14i1.15087

Abstract

This study aims to know the basic media with sago pulp supplementation which can be formulated into a medium for producing white oyster mushrooms and also to know the comparison of the right media and supplements in the formulation to give maximum oyster mushrooms production. In this study using a comparison with sawdust substrate and sago pulp to see how effective the sago pulp substrate as a supplement media for the oyster mushroom production. the program used is experimental designed in Completely Randomized Design (CRD), consists of three replications so that twelve experiments were obtained. The treatment arrangement is M1: Base media without supplements (controls), M2: 90% base media + 10% sago pulp, M3: 80% basic media + 20% sago pulp, M4: base media 70% + sago pulp 30%. The observation parameters used in the study are mycelium growth, fruit body growth, biological efficiency and measurement of protein levels in the fruit body of the oyster mushroom. The results showed that the addition of sago pulp in the media of oyster mushroom production had no effect on the growth of oyster mushrooms. This can be seen from the media of production of M1 (without the addition of sago pulp) experiencing a higher growth rate of mycelium compared to other treatments. The addition of sago pulp has an effect on the levels of oyster mushroom protein. This can be seen from the protein content of oyster mushrooms in M2 production media which have high protein content. Normal levels of white oyster mushrooms in sawdust media range from 18% -20%. This is due to the mixing of wood powder as a production medium and 10% sago pulp as supplementation to produce more nutrient levels.
POTENSI EKONOMI PEMANFAATAN LIMBAH BATANG PISANG DAN LIMBAH JERAMI JAGUNG SEBAGAI SUBSTRAT DASAR UNTUK PERTUMBUHAN MISELIUM JAMUR TIRAM PUTIH umrah umrah; I Nengah Suwastika; Lina Mahardiana; Meryany Ananda; Novika
Biocelebes Vol. 15 No. 1 (2021)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22487/bioceb.v15i1.15490

Abstract

Pertumbuhan miselium jamur tiram putih (Pleurotus ostreatus) pada substrat (batang pisang dan batang jagung) telah dilakukan di Laboratorium Mikrobiologi, Departemen Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam. Penelitian ini bertujuan untuk mengetahui: (a) pertumbuhan P. ostreatus pada substrat yang ditambahkan dengan limbah air kelapa dan limbah tempe; (b) karakteristik pertumbuhan P. ostreatus pada perlakuan berbagai jenis substrat. Penelitian ini dirancang berdasarkan Rancangan Acak Lengkap (RAL) yang terdiri dari enam perlakuan dan empat ulangan. Komposisi dari perlakuan adalah rasio antara batang pisang : batang jagung yaitu P1 (100%), P2 (80%: 20%), P3 (60%: 40%), P4 (40%: 60%), P5 (20% : 80%), P6 (100%). Hasil penelitian menunjukkan bahwa Perlakuan P6 lebih baik dibandingkan dengan perlakuan lain pada pertumbuhan miselium.
KARAKTERISTIK PERTUMBUHAN MISELIUM JAMUR TIRAM PUTIH (Pleurotus ostreatus) PADA MEDIUM DASAR JERAMI DAN TONGKOL JAGUNG umrah umrah; I Nengah Suwastika; eny Yuniati; Rifka Rifka; Nurvita Nurvita
Biocelebes Vol. 15 No. 2 (2021)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22487/bioceb.v15i2.15621

Abstract

Laju pertumbuhan miselium jamur tiram putih (Pleurotus ostreatus (Jacq) P. Kumm) dengan medium dasar jerami dan tongkol jagung telah dilaksanakan di Laboratorium Mikrobiologi Jurusan Biologi Fakultas Matematika dan Ilmu Pengetahuan Alam. Tujuan Penelitian; (a) Untuk mengamati pertumbuhan jamur tiram putih (Pleurotus ostreatus) pada formulasi media limbah jerami dan tongkol jagung; (b) Untuk mengetahui perbandingan dosis limbah jerami dan tongkol jagung yang dapat memberikan pertumbuhan terbaik terhadap jamur tiram putih. Penelitian ini didesain berdasarkan Rancangan Acak Lengkap (RAL) yang terdiri dari 7 perlakuan dan 3 kali ulangan. Susunan perlakuan merupakan perbandingan antara Serbuk jerami jagung : serbuk Tongkol Jagung yakni P1 (Serbuk Jerami 100%, tanpa Serbuk Tongkol Jagung), P2 (80% + 20%), P3 (60% + 40%), P4 (40% + 60%), P5 (20% + 80%), P6 (Serbuk Tongkol Jagung 100%, tanpa Serbuk jerami), P7 (Serbuk Gergaji 70% + Dedak Padi 20% + Tepung Jagung 10%, tanpa Serbuk Jerami dan Tongkol Jagung). Parameter pengamatan; (a) Media dasar pertumbuhan; (b) Karakteristik mikroskopik hifa; (c) Laju pertumbuhan miselium; (d) Karakteristik makroskopik koloni; (e) Masa inkubasi. Hasil penelitian menunjukkan laju pertumbuhan miselium tercepat pada perlakuan P4 yaitu 103 mm dan terendah pada perlakuan P1 yaitu 75 mm. Jumlah koloni (CFU) tertinggi pada perlakuan P7 yaitu 1,6x1012CFU/g dan terendah pada perlakuan P4 yaitu 0,2x1012CFU/g. Waktu inkubasi paling cepat pada perlakuan P7 yaitu 28 hari dan terendah pada perlakuan P4 yaitu 30 hari.
CALLUS INDUCTION AND PROPAGATION OF COCOA (Theobroma cacao L.) CLONES SULAWESI 2 IN VARIOUS CONCENTRATION OF 2,4-D, BAP ADDING WITH COCONUT WATER Haliani,Wardah,I Nengah Suwastika
AGROLAND: The Agricultural Sciences Journal Vol 2, No 2 (2015)
Publisher : Tadulako University

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Abstract

The goals of this experimen were to obtain the best concentration of 2,4-D with 15% coconut water to induced cacao callus, and the best concentration of BAP with 15% coconut water in callus propagation. This was experimental research which arranged by Completely Randomized Design, consists of two steps, callus induction and callus propagation. Callus induction from cacao flowers used several concentrations of 2,4-D combined with 15% of coconut water. In callus propagation, the medium treated by various concentrations of BAP with 15% of coconut water. This research was undertaken during period of time February until April 2016 at Laboratory of Forestry BiotecnologyForestry Faculty of Tadulako University. Data was analysed with analysis of variance and difference between treatment were determined by using BNJ at 5%. The result showed that the best medium for callus induction was 2.0 mg/L of 2,4-D with 15% of coconut water (K3). This medium produced callus in 13.50 days after induction with 99,17% of explants producing callus. Treatment of 2 mg/L of 2,4-D + 1.0 mg/L of BAP + 15 % of coconut water (SK6) was giving best response in  callus propagation. This medium produced 0.55 ml volume of callus, white-yellowish callus with crumb textured which were active in cell proliferation.
Co-Authors Agung, Muhammad Budi Akhmad Khumaidi Ali Djamhuri Anis Nurwidayati Anna Rufaida Aras, Mohammad Rezha Ari Sulistiami Asri Pirade Paserang Bill Yorsan Yonathan Cindy Claudia, Cindy Dana Sulistia Eny Yuniati Fadhalna Fadhalna Fahri Fahri Fahri Fahri Felma Tri Utami Frastika, Dian Gazali, Faris Muhammad Haliana Haliana Haliani Haliani Handayani, Mutia Harliana Harliana Haryati Baktiar Herlina Herlina Ihwan Ihwan Imelda, Silvia Jayawarsa, A.A. Ketut Jintan Jintan Laksono Trisnantoro Lina Mahardiana Marina Marina Mazidaturohmah, Mazidaturohmah Melisa S.M. Sorentina Meryany Ananda Mohammad Fahmi Musdalifah Musdalifah Muslimin Muslimin Ni Nyoman Lidyawati Niluh Made Dwi PYD Ningsi, Putri Suria Novika Nurul Aisyah Nurvita Nurvita Orryani Lambui Paserang, Asri P. Pranata, Adhi Rahmansyah Rahmansyah Rahmat Rahmat Ramadanil Pitopang Ramadhanil Pitopang Ramadhanil Pitopang Ramadhanil pitopang Rifka Rifka Riska Tajuddin Rosidah, Maya Sofiyanti Sahril M Taher Sari, Riska Sri Niken Ariati Sri Utami Talantan, Vrillia Marannu Umrah Umrah Urfiana Urfiana Waeniati Waeniati Waeniaty Waeniaty Weaniati Weaniati Wilma Wilma Yundari, Yundari Yunus, Fahrunnisa Yuzammi Yuzammi