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All Journal Jurnal Penelitian Pertanian Terapan BIOEDUSCIENCE Agrin : Jurnal Penelitian Pertanian Agritrop : Jurnal Ilmu-Ilmu Pertanian (Journal of Agricultural Science) Paspalum: Jurnal Ilmiah Pertanian JURNAL BUDIDAYA PERTANIAN Jurnal Penelitian IPTEKS Jurnal Penelitian Ilmu Sosial dan Eksakta PEKAT : Jurnal Pengabdian Kepada Masyarakat National Multidisciplinary Sciences DIANKES : Jurnal Pengabdian Teknologi Informasi dan Kesehatan International Applied Science Callus: Journal of Agrotechnology Science BIOSAPPHIRE: Jurnal Biologi dan Diversitas Agrimas : Jurnal Pengabdian Masyarakat Bidang Pertanian
Hidayah Murtiyaningsih
Universitas Muhammadiyah Jember
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Civil Engineering, Building, Construction & Architecture Computer Science & IT Dentistry Education Electrical & Electronics Engineering Engineering Environmental Science Health Professions Immunology & microbiology Law, Crime, Criminology & Criminal Justice Mathematics Medicine & Pharmacology Nursing Public Health Social Sciences Veterinary Other

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Journal : BIOEDUSCIENCE

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Pengukuran Indole- 3-Acetic Acid (IAA) pada Bacillus sp dengan Penambahan L-Tryptopan Astriani, Meli; Murtiyaningsih, Hidayah
BIOEDUSCIENCE Vol 2 No 2 (2018): BIOEDUSCIENCE
Publisher : Universitas Muhammadiyah Prof. Dr. Hamka

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (287.055 KB) | DOI: 10.29405/j.bes/22116-1212233

Abstract

Background: Indole acetic acid (IAA) is a plant growth hormone that has an important role for stimulation growth of plants. The exogenous IAA produced by bacteria is able to accelerate the plant growth in improving process of root differentiation to form root hairs. Rhizosphere bacteria mostly known as a producer of IAA. Bacillus sp is one of rhizosphere bacteria on plants. The aim of this study was to determine IAA content from Bacillus sp by measuring the levels of IAA using colorimetric method. Methods: Stages of this study are as follows: recultures of testing isolates, manufactures IAA standard curve and measures levels of IAA which reacted to Salkowsky reagent. Results: Results showed that IAA content of Bacillus sp which were obtained by adding L- tryptophan was 39.92 ppm. Conclusions: IAA content which produced by Bacillus sp is high enough to be applied as a plant growth promoter bacteria (PGPR).
Pengukuran Indole- 3-Acetic Acid (IAA) pada Bacillus sp dengan Penambahan L-Tryptopan Meli Astriani; Hidayah Murtiyaningsih
BIOEDUSCIENCE Vol 2 No 2 (2018): BIOEDUSCIENCE
Publisher : Universitas Muhammadiyah Prof. Dr. Hamka

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (287.055 KB) | DOI: 10.29405/j.bes/22116-1212233

Abstract

Background: Asam indol asetat (IAA) merupakan salah satu hormon pertumbuhan tanaman yang berperan penting dalam menstimulasi pertumbuhan tanaman. Peran IAA yang diproduksi eksogen dari bakteri mampu mempercepat pertumbuhan tanaman dalam memacu proses diferensiasi pada akar dalam membentuk rambut akar. Bakteri rizosfer kebanyakan sebagai penghasil IAA. Bacillus sp. merupakan salah satu bakteri rizosfer dari tanaman. Tujuan penelitian ini untuk mengetahui kandungan IAA dari Bacillus sp. dengan melakukan pengukuran kadar IAA menggunakan metode kolorimetri Metode: Tahapan penelitian meliputi peremajaan isolat uji, pembuatan kurva standar IAA dan mengukur kadar IAA dengan direaksikan menggunakan reagen Salkowsky. Hasil: Hasil dari penelitian ini diperoleh kandungan IAA dari kultur Bacillus sp. yang ditambahkan L-triptofan yaitu sebesar 39,92ppm. Kesimpulan: Kandungan IAA yang dihasilkan Bacillus sp. tergolong cukup tinggi untuk dapat diaplikasikan sebagai bakteri pemacu pertumbuhan tanaman (PGPR).
Co-Authors Ahmad Rizki Junaedi Akbar Rafsanjani Anggriawan, Rendy Arum, Laras Sekar Bagus Tripama Bejo Suroso Bejo Suroso Binaridha Kusuma Ningtyas Ega Dwi Sukmadiningsih Fatechan, Billy Fodesta, Fiana Galuh Widya Nanda Gracia Melsiana Aldini Hadianto, Mohamad Gozali Hasbi, Hudaini Hazmi, M. Hazmi Hazmi, Muhammad Hendriyanto, Aldi Hudaini Hasbi Junaedi, Ahmad Rizki Kumara Hadi, Danang Laras S Arum Laras Sekar Arum Laras Sekar Arum Laras Sekar Arum Levinia Wuri Safitri M. Luthfi Ryzaldi Mega Saniya Juli Nuriyatul Mega Silvia Meli Astriani meli Astriani Muhammad Hazmi Muliasari, Risa Martha Nugroho, Tito Agung Oktarina Oktarina Oktarina, Oktarina Prasetyo, Vandi Putranto Rahayu, Reni Puji Ramadhan, Moch. Alfian Rizky Restanto, Didik Puji Rima, Baidhatul Khoirimah Siti Aisyah Suroso, Bejo Tripama, Bagus Umarie, Iskandar Utami, Erisa Putri