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All Journal Jurnal Veteriner Journal of Parasite Science Journal of Applied Veterinary Science and Technology
Kuncoro Puguh Santoso
Department Of Basic Veterinary Medicine - Faculty Of Veterinary Medicine, Universitas Airlangga
Agriculture, Biological Sciences & Forestry Immunology & microbiology Veterinary

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Aktivitas Superoxide Dismutase pada Serum Darah Babi Landrace yang Disembelih dengan Metode Electrical Stunning Shabrina Fauzia Prayoga; Kuncoro Puguh Santoso; Nove Hidajati; Faisal Fikri; Maya Nurwartanti Yunita; Muhammad Thohawi Elziyad Purnama
Jurnal Veteriner Vol 22 No 2 (2021)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (147.263 KB) | DOI: 10.19087/jveteriner.2021.22.2.168

Abstract

Tujuan penelitian ini untuk menentukan aktivitas Superoxide Dismutase (SOD) pada babi Landrace yang disembelih dengan metode stunning dan non-stunning. Sebanyak 18 ekor babi Landrace jantan dewasa yang telah dikebiri dengan masing-masing bobot 115±20 kg dibagi menjadi dua kelompok, yakni stunning dan non-stunning dengan pengujian serum pada pre dan post treatment. Electrical stunning dilakukan dengan aliran listrik pada dua elektroda sebesar 220 volt dan 1,3 ampere. Sampel darah diambil sebanyak 3 mL, kemudian diuji aktivitas SODnya.. Hasil aktivitas SOD dianalisis dengan uji T berpasangan untuk membandingkan aktivitas SOD serum antara stunning dan non-stunning pada fase pre dan post treatment. Hasil stunning menunjukkan penurunan signifikan (p<0,05) aktivitas SOD sebesar 56,12±54,47 dibandingkan non-stunning sebesar 137,84±66,63. Aktivitas SOD yang rendah tampak signifikan (p<0,05) juga pada pre-treatment dibandingkan dengan post-treatment. Dapat disimpulkan bahwa penyembelihan dengan metode electrical stunning menurunkan aktivitas SOD pada serum babi Landrace.
Prevalence of Nematodiasis in Omasum and Abomasum of Goats Slaughtered at Pegirian Surabaya Slaughter House Through Gatrointestinal Surgery Method Dewi Mariyam; Sri Mumpuni Sosiawati; Kuncoro Puguh Santoso; Setiawan Koesdarto; Sarmanu Sarmanu; Ira Sari Yudaniayanti
Journal of Parasite Science (JoPS) Vol. 2 No. 1 (2018): Journal of Parasite Science
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (533.67 KB) | DOI: 10.20473/jops.v2i1.16384

Abstract

The purpose of this research is to determine the kind of nematodes and to determine the prevalence result. Thirtysix samples of omasum and abomasum were collected from RPH Pegirian from September-November 2017 then using gastrointestinal surgery to get fresh adult worm, afterwards using Semichen-Acetic Carmine stainning method to makes the object easy to microscopic identify. The result showed that species was found Haemonchus contortus, as an identify result show that adult worm has a character on a anterior part called cervical papilae, female  worm has a barber’s pole on body also posterior part has a vulva flap, posterior part of male worm has a bursa copulatrix, spicula, gubernaculum and dorsal rays looks like Y shape. Seventeen positive samples and the prevalence rate was 47,2%, 273 adult worms and female worms 215 (78,75%) and the male worms 58 (21,25%). Chi-Square statistical test (P <0.05) showed that 13 positive samples of kambing kacang (65%) were higher infected Haemonchosis infection than 4 potitive samples of PE (25%).
Microscophy Identification of Toxocara cati First Stage Larvae and Second Stage Larvae Eny Coolfina Simarmata; Kusnoto Kusnoto; Mochamad Lazuardi; Setiawan Koesdarto; Endang Suprihati; Kuncoro Puguh Santoso
Journal of Parasite Science (JoPS) Vol. 3 No. 1 (2019): Journal of Parasite Science
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (223.447 KB) | DOI: 10.20473/jops.v3i1.16420

Abstract

This study was aimed to identify the ultra structural morphology of Toxocara cati First Stage Larvae and Second Stage Larvae using Light Microscope. Toxocara cati larvae were obtained from adult worm eggs then were treated in phospat buffer saline with comparasion 1 : 1 until it reached the stage of larvae 1 and 2. The shell of egg Toxocara cati were thick and usually consist of three layers. The first layer was inner membrane, the second layer was middle layer and the third was outer layer. The results of larvae morphology that were identified using light microscope showed that the anterior part of Toxocara cati first stage larvae has a dorsal lip and esophagus and intestine on the posterior part but it could not be identified perfectly. The anterior part of Toxocara cati second stage larvae that were identified has three lips that leads directly into the oesophagus. Three lips on the anterior part of Toxocara cati larvae 2 consist of a dorsal lip and two subventral lips. Morphological of Toxocara cati larvae similar with the Toxocara cati adult worms because morphology of larvae and adult stage was difficult to differentiated.
Detection of Salmonella sp. on Layer Chicken Eggs Sold at The Vegetable Market of Magetan Regency Brillian Inawulan Syamsiar; Kuncoro Puguh Santoso; Ira Sari Yudaniayati; Diyantoro Diyantoro
Journal of Applied Veterinary Science And Technology Vol. 1 No. 2 (2020): October 2020
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/javest.V1.I2.2020.34-38

Abstract

Background: Eggs are poultry products that cannot be separated from the possibility of Salmonella contamination. Traditional markets are places where consumers can get chicken eggs with poor hygiene and sanitation aspects. Habits of today's society that often consumes raw or undercooked eggs can trigger Salmonellosis. Purpose: To identify the presence of Salmonella sp. in eggs sold in Magetan Regency Vegetable Market. Method: Sampling of eggs from different random stores. The eggs then proceeded with selection pre-enrichment, selective enrichment, isolation, and identification. Results: The handling of eggs in the Magetan Regency Market was quite good in its storage period, and purebred eggs sold in the Magetan Regency Market were negatively contaminated with Salmonella sp. Conclusion: Broiler chicken eggs sold in Magetan Vegetable Market are safe to consume.
Co-Authors Arif Nur Muhammad Ansori Astria Novitasari Nidom Balqis Afifah Brillian Inawulan Syamsiar Dewi Mariyam Dhani Prakoso Diyantoro Diyantoro, Diyantoro Endang Suprihati Eny Coolfina Simarmata Faisal Fikri Imam Mustofa Ira Sari Yudaniayanti Ira Sari Yudaniayati Kusnoto Kusnoto Mochamad Lazuardi Muhammad Khaliim Jati Kusala Muhammad Thohawi Elziyad Purnama Nidom, Chairul Anwar Nor Laela Ramadhaniyah Nove Hidajati Rahmahani, Jola Reviany Vibrianita Nidom Sarmanu, Sarmanu Setiawan Koesdarto Setyarina Indrasari Shabrina Fauzia Prayoga Soeharsono Soeharsono Sri Mumpuni Sosiawati Yunita, Maya Nurwartanti