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All Journal ACTA VETERINARIA INDONESIANA Hemera Zoa Jurnal Sain Veteriner Jurnal Veteriner Jurnal Ilmu Ternak Veteriner JURNAL KAJIAN VETERINER Jurnal Kedokteran Hewan
Agnesia Endang Tri Hastuti Wahyuni
Departemen Mikrobiologi, Fakultas Kedokteran Hewan, Universitas Gadjah Mada, Yogyakarta
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Public Health Veterinary

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Journal : Jurnal Veteriner

 1 
Protektivitas Sapi di Kabupaten Kupang Terhadap Penyakit Ngorok (Septicaemia Epizootica) (PROTECTIVITY AGAINST SEPTICAEMIA EPIZOOTICA OF COWS IN KUPANG DISTRICT) Hilda Susiyanti Debora Berek; Widagdo Sri Nugroho; Agnesia Endang Tri Hastuti Wahyuni
Jurnal Veteriner Vol 16 No 2 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Controlling SepticemiaEpizooticae (SE) through vaccination program has been undertaken in KupangDistrict. However, numbers of fatal cases is still being reported. A cross sectional study have been undertakenin order to determine the prevalence and factors affecting herd immunity at farm level in Kupang District.A total of 434 cow’s sera from 87 farmers were collected and further analyzed using Enzyme-linkedImmunosorbent Assay (ELISA). An antibody titer of e”200 ELISA Unit was used as the indicator ofprotective immunity. The prevalence of herd immunity was 73.7% and vaccination coverage was 90.8%.Based on unweighted logistic regression analysis it was found that factors affecting the animals protectiveimmunity were: animal >2 years of age (r = +1.45601; OR = 4.3); housing system ( r = +1.03958; OR = 2.8);reservoir animals (r = +0.090147; OR = 2.5), the sex of the animal (female) (r = + 0.080138; OR =2.2); oncefrequency of vaccination (r = +0.61015; OR = 1.8); and 6-12 months post vaccination period (r = + 0.58968;OR = 1.8). Based on linier regression analysis, the prevalence of herd immunity was increased during rainyseason, whereas it was decreased when cows werebought from animal market or from other district.
Faktor-Faktor Risiko Mastitis Subklinis pada Kambing Peranakan Etawah di Kabupaten Sleman, Yogyakarta (RISK FACTORS OF SUBCLINICAL MASTITIS ON ETTAWA CROSSBRED GOAT IN SLEMAN REGENCY, YOGYAKARTA) Widodo Suwito; Widagdo Sri Nugroho; Bambang Sumiarto; Agnesia Endang Tri Hastuti Wahyuni
Jurnal Veteriner Vol 15 No 1 (2014)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

In Sleman, a regency in Yogyakarta special region, Etawah crossbred goats are excessively bred for thedairy produce called the goat’s milk. Subclinical mastitis is one of diseases which reduce the yield of goat’smilk. The aim of this study was to determine the risk factors that contribute to the subclinical mastitis onthe Etawah crossbred goats in Sleman. The 200 samples one of which contains 10 mL of goat’s milk weretaken from the udders of the Etawah crossbred goats from the eight goat farms in Sleman. The 200samples were analyzed for the subclinical mastitis using California Mastitis Test (CMT). The data of riskfactors were gathered through a questionnaire. The risk factors on Etawah crossbred goats in Sleman weredetermine with the use of bivariate analysis chi square (X)2, odds ratio (OR) and relative risk (RR). Thegoat’s milk which subclinical mastitis was isolation and identification of bacteria based on biochemicaltests. The risk factors that cause the subclinicall mastitis on Etawah crossbred goats in Sleman were (1)milk yield (X2=14.23; OR=6.52; RR=4.42), (2) age status of lactation (X2=1.60; OR=59.09; RR=17.94), (3)age of weaning (X2=26.06; OR=2.22; RR=1.91), and (4) Body Condition Score (BCS) (X2=13.89; OR=1.29;RR=1.22). Goat’s milk which subclinicall mastitis were isolated  Bacillus sp  (70%), Staphylococcus sp(33%), Pseudomonas sp (29%), Streptococcus sp (25%), Corynebacterium sp (12%), and E. coli (4%).
Aktivitas Antibakteri Ekstrak Daun Pepaya, Daun Kemangi Serta Temu Ireng, dan Madu terhadap Bakteri Serratia marcescens (ANTIBACTERIAL ACTIVITY OF PAPAYA LEAVES, BASIL LEAVES AND CURCUMA AERUGINOSA EXTRACT AND HONEY AGAINST SERRATIA MARCESCENS) Yovita Devina; Vinsa Cantya Prakasita; Dwi Cahyo Budi Setiawan; Agnesia Endang Tri Hastuti Wahyuni
Jurnal Veteriner Vol 21 No 2 (2020)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Antibiotic Growth Promoters (AGPs) are antibiotics that are used commonly in livestock farming to increase animals growth rate. The use of AGP has been prohibited in Indonesia (No.14/Permentan/PK.350/ 5/2017). AGP banning urges some innovations to find the alternative of AGP and one of them is utilization of natural resources. Papaya leaves, basil leaves, Curcuma aeruginosa rhizomes and honey contain flavonoid that has antibacterial activity. The purpose of this research is to know the effect of Papaya leaves, basil leaves, Curcuma aeruginosa rhizomes and honey against Serratia marcescens growth. Re-identification of Serratia marcescens were done by looking at the colony morphology, cell morphology and biochemical tests. Antibacterial activity of ethanol (100%) and aquades (33.33%) extract of the herbals and the honey (100%) against Serratia marcescens were tested by disc diffusion method. Each test was repeated 2 times. The results showed that Lanceng (Trigona bee) honey from Gunung Kidul, Black honey from Lombok, White honey from Lombok, ethanol and aquades extract of the herbals are not effective to inhibit Serratia marcescens’s growth. Commercial honey (7.59±0.22 mm) has the highest antibacterial acivity to Serratia marcescens, followed by honey from Kupang (6.69±0.21 mm). Commercial honey and honey from Kupang have moderate antibacterial activity. It can be cocluded that comercial honey and honey from Kupang can ihibit Serratia marcescens’s growth
Analisis Genetik Gen Protective Antigenic pada Bacillus anthracis Isolat Jawa Tengah dan Yogyakarta (GENETIC ANALYSIS ON PROTECTIVE ANTIGENIC GENE OF BACILLUS ANTHRACIS ISOLATES OF CENTRAL JAVA AND YOGYAKARTA) Maxs Urias Ebenhaizar Sanam; Widya Asmara; Agnesia Endang Tri Hastuti Wahyuni; Michael Haryadi Wibowo
Jurnal Veteriner Vol 16 No 1 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The aim of the study was to determine sequence and genotype diversity of protective antigenic gene ofBacillus anthracis isolated from Central Java and Yogyakarta. Pag-A gene which encodes for antigenicprotein is one toxin component and the virulent factor of B. anthracis. As many as five isolates fromSemarang, Sragen, and Boyolali (Central Java) and Sleman (Yogyakarta) were used. The gene wassequenced and amplified used three set of primers PA1857/PA2436, PA8/PA5, and PA-5F/PA-5R. Theresult showed that the nucleotide sequences of gene from five isolates were identical and only had onenucleotide difference as compared to B. anthracis sterne M22589. All isolates were confirmed as genotypebased on pag-A sequence. It was concluded that all B. anthracis from Central Java and Yogyakarta haveidentical pag-A sequence and belong to genotypt-1. Further studies are needed to investigate B. anthracisisolates from other regions of Indonesia.
Resistansi Antibiotik Bakteri dari Ulas Kloaka Burung Puyuh Sehat Maria Anggita; Widya Asmara; Tri Untari; Michael Haryadi Wibowo; Sidna Artanto; Okti Herawati; Agnesia Endang Tri Hastuti Wahyuni
Jurnal Veteriner Vol 22 No 4 (2021)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (123.172 KB) | DOI: 10.19087/jveteriner.2021.22.4.508

Abstract

Anti Mikrob Resistan (AMR) menjadi masalah utama baik pada manusia, hewan, dan lingkungan. Pada umumnya pemberian antibiotik dilakukan oleh peternak unggas di Indonesia melalui pakan sebagai antibiotik pemacu pertumbuhan/Antibiotic Growth Promoters (AGP). Penggunaan antibiotik yang berlebihan di industri peternakan dianggap berkontribusi terhadap meningkatnya resistansi obat pada manusia. Penelitian ini bertujuan untuk mengetahui kejadian resistansi bakteri dari ulas/swab kloaka burung puyuh sehat. Sebanyak sepuluh ekor puyuh sehat dari peternakan puyuh di daerah Kalasan, Klaten, Yogyakarta diswab kloaka dan dikultur pada media cair Brain Heart Infusion (BHI). Biakan ditanam pada media Mueller Hinton Agar (MHA) dan diletakkan Sembilan jenis cakram/disk antibiotik: streptomisin, doksisiklin, fosfomisin, kloramfenikol, kolistin, siprofloksasin, ampisilin, eritromisin, dan penisilin. Setelah inkubasi pada suhu 37°C selama 18-24 jam, zona hambat yang terbentuk kemudian diukur dan ditentukan sifat resistansi dibandingkan dengan standar. Hasil menunjukkan sebanyak 20%kultur bakteri resistan terhadap streptomisin, 40% resistan terhadap doksisiklin, 40% resistan terhadap kloramfenikol, 50% resistan terhadap kolistin, 20% resistan terhadap siprofloksasin, 20% resistan terhadap ampisilin, 90% resistan terhadap eritromisin, 50% resistan terhadap penisilin, dan tidak ada resistansi terhadap fosfomisin. Terdapat satu dari sepuluh puyuh (P10) yang memiliki resistansi terhadap tujuh dari sembilan jenis antibiotik (78%) yang diujikan, dan dua dari sepuluh puyuh (P2 dan P4) memiliki resistansi terhadap dua dari sembilan antibiotik (11%). Hasil penelitian menunjukkan bahwa bakteri dari swab kloaka pada burung puyuh sehat umur 21 hari dari satu peternakan yang sama memiliki tingkat resistansi yang berbeda-beda. Sifat resistansi terhadap antibiotik dari masing-masing puyuh juga berbeda-beda.
Histopatologi Ikan Kerapu Macan yang Diimbuhi Bakteri Asam Laktat dan Diuji Tantang Vibrio alginolyticus (HISTOPHATOLOGY OF TIGER GROUPER SUPPLEMENTED WITH LACTIC ACID BACTERIA AND CHALLENGED BY VIBRIO ALGINOLYTICUS) Nursyirwani .; Widya Asmara; Agnesia Endang Tri Hastuti Wahyuni; Triyanto .
Jurnal Veteriner Vol 16 No 4 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (291.771 KB)

Abstract

Supplementation of lactic acid bacteria (LAB) as probiotic in aquaculture has been reported toincrease fish growth and enhance their resistance against diseases. The aim of this study was to figureout histological changes of tiger grouper (Epinephelus fuscoguttatus) fed with LAB isolates followed bycha
Aktivitas Antibakteri Ekstrak Daun Pepaya, Daun Kemangi Serta Temu Ireng, dan Madu terhadap Bakteri Serratia marcescens Agnesia Endang Tri Hastuti Wahyuni; Yovita Devina; Vinsa Cantya Prakasita; Dwi Cahyo Budi Setiawan
Jurnal Veteriner Vol 23 No 4 (2022)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19087/jveteriner.2022.23.4.465

Abstract

Antibiotic Growth Promoters (AGPs) are antibiotics that are used commonly in livestock farming to increase animals growth rate. The use of AGP has been prohibited in Indonesia (No.14/PERMENTAN/PK.350/5/2017). AGP banning urges some innovations to find the alternative of AGP and one of them is utilization of natural resources. Papaya leaves, basil leaves, Curcuma aeruginosa rhizomes and honey contain flavonoid that has antibacterial activity. The purpose of this research is to know the effect of Papaya leaves, basil leaves, Curcuma aeruginosa rhizomes and honey against Serratia marcescens growth. Re-identification of Serratia marcescens were done by looking at the colony morphology, cell morphology and biochemical tests. Antibacterial activity of ethanol (100%) and aquades (33.33%) extract of the herbals and the honey (100%) against Serratia marcescens were tested by disc diffusion method. Each test was repeated 2 times. The results showed that Lanceng (Trigona bee) honey from Gunung Kidul, Black honey from Lombok, White honey from Lombok, ethanol and aquades extract of the herbals are not effective to inhibit Serratia marcescens growth. Commercial honey (7.59±.22 mm) has the highest antibacterial acivity to Serratia marcescens, followed by honey from Kupang (6.69±.21 mm). Commercial honey and honey from Kupang have moderate antibacterial activity.
Co-Authors Adrenalin, Sruti Listra Agustina Dwi Wijayanti Anak Agung Gede Sugianthara Arfian Rahma Shanti Bambang Sumiarto Bambang Sumiarto Bambang Sumiarto Charles Rangga Tabbu Charles Rangga Tabbu Charles Rangga Tabu Dito Anggoro Ery Haryani Fauziah, Ima Herawati, Okti Hilda Susiyanti Debora Berek I wayan Teguh Wibawan Ima Fauziah Ima Fauziah Imanjati, Lynda Nugrahaning Liza Angeliya Lynda Nugrahaning Imanjati Lynda Nugrahaning Imanjati Maria Anggita Marla Anggita Marlin Cindy Claudya Malelak Marseni Banne Ringgi Maxs Urias Ebenheizer Sanam Michael Haryadi Wibowo Nursyirwani, Nursyirwani Nyoman Reishita Andriyani Pasha Glabella Radhian Fadiar Rahmat Setya Adji Rahmat Setya Adji Salma Ramandhanti Putri Santoso, Ferdinand Prayogo Cahyo Setiawan, Dwi Cahyo Budi Setyawan Budiharta Sidna Artanto Sidna Artanto Sidna Artanto Sitarina Widyarini Sitarina Widyarini Sruti Listra Adrenalin Sruti Listra Adrenalin Surya Amanu Surya Amanu Tangkonda, Elisabet Tati Aryani Tri Untari Tri Untari Triyanto . Vinsa Cantya Prakasita Widagdo Sri Nugroho Widagdo Sri Nugroho Widodo Suwito Widodo Suwito Widodo Suwito Widya Asmara Widya Asmara Yovita Devina Yovita Devina