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All Journal Jurnal Ilmu dan Kesehatan Hewan Buletin Veteriner Udayana Jurnal Veteriner Indonesia Medicus Veterinus Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Journal of the Indonesian Tropical Animal Agriculture
I Gusti Ngurah Bagus Trilaksana
Laboratorium Reproduksi Veteriner Fakultas Kedokteran Hewan Universitas Udayana
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Earth & Planetary Sciences Health Professions Immunology & microbiology Medicine & Pharmacology Public Health Veterinary

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Seks Rasio pada Anak Tikus yang Dilahirkan Akibat Pemberian Bee Pollen Kresensia Cyntia Dosom; Desak Nyoman Dewi Indira Laksmi; I Gusti Ngurah Bagus Trilaksana
Buletin Veteriner Udayana Vol. 15 No. 2 April 2023
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2023.v15.i02.p13

Abstract

The purpose of this study was to see how effective bee pollen is at determining sex in rats. This was an experimental study with three treatments and control group that used a completely randomized design. There was no bee pollen in control group. Then in P1, only female rats were given bee pollen (18mg), in P2 only male rats (22mg), and in P3 both male and female rats were given the same dose of bee pollen. Male and female rats were mated after receiving bee pollen for 49 days, and the sex ratio of the newborn rats was calculated after 21 days of gestation. ANOVA was used to analyze th data before proceeding with Duncan’s test. The findings of this study revealed that bee pollen could increase the proportion of male rats. This is because giving bee pollen to male rats allows more Y-chromosome spermatozoa to be produced and survive in this condition in the female reproductive tract. Andosperms benefit from this condition because they can move faster and more agilely than gynosperma. More male offspring are produced because the Y sperm (andosperm) or male sperm first reaches the egg and fertilizes it. Giving male and female rats bee pollen at the same time can increase the sex ratio of male rat pups.
Penyuntikan Gonadorelin pada Saat Estrus Terhadap Perkembangan Folikel dan Terjadinya Ovulasi serta Non Return Rate pada Sapi Bali yang Mengalami Kawin Berulang Gusde Wahyu Krisna Suputra; I Gusti Ngurah Bagus Trilaksana; Tjok Gde Oka Pemayun; I Wayan Sukernayasa; I Nyoman Oka Widiarta
Buletin Veteriner Udayana Vol. 15 No. 2 April 2023
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2023.v15.i02.p11

Abstract

This study aims to prove that gonadorelin administration is able to cause follicle development to reach follicles that are ready to be ovulated and ovulation and fertilization occurs in bali cattle that experience repeated breeder in Sobangan Village, Badung Regency, Bali. Observations of developing follicles and the timing of ovulation were performed using transrectal ultrasound via rectal palpation. The design used in this study was a completely randomized design (CRD) consisting of three treatment groups, namely the control group without gonadorelin injection (P0), the group receiving 50 g/im/head injection of gonadorelin (P1) and the group receiving 50g/im/head injection. gonadorelin injection at a dose of 100g/im/head (P2). Gonadorelin injection was performed when clear discharge came out of the vagina as a sign of the appearance of estrus and all samples in IB at 12 hours after the appearance of estrus. The results showed that the average follicle diameter at the time of estrus in the P0 group was 7.37+0.46mm, the P1 group was 7.67+0.78mm, and the P2 group was 7.59+0.77mm and statistically did not show any difference. which meaningful (P> 0.05). When reaching the de graff follicle, the mean follicle diameter in the P0 group was 11.27+0.59mm, the P1 group was 10.08+0.60mm, the P2 group was 9.92+1.06mm statistically showed a significant difference (P<0.05). At ovulation the mean follicle diameter in the P0 group was 10.53+0.57mm, in the P1 group 9.51+0.59mm, in the P2 group 9.33+0.89mm, statistically showed a significant difference (P<0.05). Ovulation time in the P0 group was 71.56+1.33 hours, while for the P1 and P2 groups it was 12.00+0.00 hours and statistically showed a significant difference (P<0.05). The percentage of non-return rates in the three treatment groups were 0%, 77.8% and 88.9%, for P0, P1 and P2, respectively.
Penambahan Vitamin E pada Pengencer SemenLife® Terhadap Kualitas dan Lama Simpan Spermatozoa Babi Landrace Gede Putra Sanjaya; Wayan Bebas; Tjok Gde Oka Pemayun; I Gusti Ngurah Bagus Trilaksana; Desak Nyoman Dewi Indira Laksmi; Ni Nyoman Werdi Susari
Buletin Veteriner Udayana Vol. 15 No. 6 December 2023
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2023.v15.i06.p16

Abstract

Artificial insemination in pigs in Indonesia still uses liquid semen. Therefore, specific media are needed to maintain the quality of pig spermatozoa. This study aims to determine the effect of adding vitamin E (a-tocopherol) to Semenlife® (INTTEC) diluent on the quality and shelf life of spermatozoa of landrace pigs. The sample used was fresh semen which was collected using a mass technique taken from the UPTD. Regional Artificial Insemination Center, Livestock Breeding and Forage/Livestock Institute of Bali Province. A total of 24 samples consisting of 12 samples in the SemenLife® (INTTEC) diluent and 12 samples in the SemenLife® (INTTEC) diluent + vitamin E 400 µg/ml were stored at 15-18oC. Observation of spermatozoa quality (viability and motility) and storage time was carried out for 120 hours with observations of spermatozoa quality under a microscope every 24 hours, then DNA fragmentation was tested using the Pocrine 8-Hydroxy-desoxyguanosine ELISA Kit. The graph results of Duncan's test showed that the addition of 400 µg/ml vitamin E had a significant effect on the quality of spermatozoa, especially in terms of motility which was maintained at 48 hours and the lowest DNA fragmentation occurred at 72 hours. Furthermore, the addition of vitamin E was not able to extend the shelf life of landrace pig spermatozoa in diluent. Where, there was a decrease in motility and an increase in DNA fragmentation, respectively at 72 and 96 hours, in viability the decrease occurred periodically from the 0 hour to 120 hours. From the results of the quality and shelf-life tests obtained, spermatozoa can then be tested by conducting a fertility test to find out whether the results obtained can increase litter size.
Pengaruh Lama Simpan Semen Ayam Ekor Panjang dengan Pengencer Kuning Telur dan Air Kelapa Wulung pada Suhu 5oC Dyah Risma Aprilia Enji; Wayan Bebas; I Gusti Ngurah Bagus Trilaksana
Buletin Veteriner Udayana Vol. 15 No. 6 December 2023
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2023.v15.i06.p19

Abstract

Long-tailed chickens or commonly called onagadori chickens are classified as rare and endangered animals. This ornamental chicken has the advantage of its long tail reaching 10 meters. This study aims to determine the effect of storing semen with egg yolk coconut water diluent stored at cold temperatures on motility, abnormalities and viability. The design in this study used a completely randomized design (CRD) with 7 treatment groups for storage duration, namely 0 hours, 12 hours, 24 hours, 26 hours, 48 hours, 60 hours, 72 hours. The research object used was male long-tailed chickens that were sexually mature at the age of 6-8 months. Chicken semen storage technique by massaging the chicken's back (massage). Semen examination was carried out macroscopically and microscopically. Viability and abnormality of spermatozoa were observed using the eosin negrosin staining method. This study concluded that the motility, viability and spermatozoa abnormalities of long-tailed chickens in egg yolk coconut water diluent could be maintained for 48 hours at 5°C storage temperature with 52.41% motility, 50.15% viability and 7.33% abnormality. In order to obtain optimal results in supporting the success of artificial insemination, long-tailed chicken semen with egg yolk diluent and coconut water should not be stored for more than 60 hours.
Co-Authors Achmad Noerkhaerin Putra AJI WAHYU WICAKSONO Apriliana, Kadek Soma Apriliani, Karolina Desak Nyoman Dewi Indira Laksmi Desak Nyoman Dewi Indira Laksmi DEVI INDRAWATI Drystiana Yessi Ayu Lesmono Dyah Risma Aprilia Enji Dyonesia Dimitri Maya Mayesta Ester Novitasari Fania, Bella Gede Putra Sanjaya Gunawan, I. W. N. F. Herdi Wahyu Adi Prananda Heros, Made Novi Lerianti I Gede Hendi Saputra I Gusti Ngurah Agung Dolly Octa I Ketut Puja I Ketut Suatha I Ketut Suatha I Nyoman Oka Widiarta I Nyoman Oka Widiarta I Putu Agus Kertawirawan I Putu Sudana Agustina I Putu Suparman I Wayan Bebas I Wayan Gorda I WAYAN PUTRA ARIMBAWA I Wayan Sukernayasa imam sobari Komang Gita Permana Kresensia Cyntia Dosom Kristyari, Ni Putu Gita Laba Mahaputra Laksmi, D. N. D. I Luh Made Sudimartini MADE KOTA BUDIASA Mahasanti, I Gusti Ayu Puji Ni Ketut Suartini Ni Komang Asni Ni Komang Sri Puspaningsih Ni Made Dwi Permata Sari Ni Nyoman Werdi Susari Priharyanthi, Luh Komang Ayu Puteri Rahel Situmorang Rinna Noviyati Ndun Rudy Lontoh Sandra, Arista Novi Saridewi, I Gusti Ayu Made Suputra, Gusde Wahyu Krisna Teja, Dewa Ngakan Gede Surya Tjok Gde Oka Pemayun Tjok Gde Oka Pemayun Tjok Gde Oka Pemayun Tjok Gede Oka Pemayun Wayan Bebas Widiastuti, Wayan Arni Yudiani, Putu Mira