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All Journal Jurnal Ilmu dan Kesehatan Hewan Indonesia Medicus Veterinus Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Journal of the Indonesian Tropical Animal Agriculture Buletin Veteriner Udayana
I Gusti Ngurah Bagus Trilaksana
Laboratorium Reproduksi Veteriner Fakultas Kedokteran Hewan Universitas Udayana
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Health Professions Immunology & microbiology Medicine & Pharmacology Public Health Veterinary

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Waktu Munculnya Birahi Pascamelahirkan pada Sapi Bali di Desa Galungan, Sawan, Buleleng, Bali Yudiani, Putu Mira; Trilaksana, I Gusti Ngurah Bagus; Laksmi, Desak Nyoman Dewi Indira
Indonesia Medicus Veterinus Vol 10 (6) 2021
Publisher : Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19087/imv.2021.10.6.896

Abstract

Penelitian ini bertujuan untuk mengetahui waktu munculnya birahi pascamelahirkan/estrus postpartum pada sapi bali yang dipelihara di Desa Galungan. Penelitian ini dilaksanakan dari bulan Desember 2020 hingga Februari 2021 di Desa Galungan, Kecamatan Sawan, Buleleng, Bali. Penelitian ini menggunakan 91 ekor sapi betina (primipara dan pluripara). Data yang didapat kemudian ditabulasikan dan dianalisis secara deskriptif. Hasil analisis didapat bahwa manajemen kandang oleh peternak sapi bali di Desa Galungan yaitu kandang tunggal sebanyak 93,38%, kandang terbuka 69,42%, dan tidak adanya pejantan yaitu 52,06%. Rata-rata estrus postpartum kelahiran pertama yaitu 3,8 ± 1,55 bulan, kelahiran kedua yaitu 3,52 ± 1,48 bulan, kelahiran ketiga yaitu 3,37 ± 1,46 bulan, kelahiran keempat yaitu 3,36 ± 1,42 bulan, kelahiran kelima yaitu 3,35 ± 1,32 bulan, kelahiran keenam yaitu 3,42 ± 1,07 bulan, kelahiran ketujuh yaitu 3,75 ± 1,32 bulan, dan kelahiran kedelapan yaitu 4,33 ± 1,67 bulan. Kelahiran kedua hingga kelima pada estrus postpartum lebih cepat dibandingkan kelahiran pertama, dan estrus postpartum keenam hingga kedelapan mulai lambat. Komposisi pakan yang diberikan oleh peternak ke sapi-sapi mereka yaitu 50% rumput gajah, 19% rumput sertaria, dan 15% daun gamal. Sebagian besar peternak hanya mengandalkan pakan hijauan dan tidak menggunakan pakan tambahan. Rata-rata estrus postpartum kelahiran pertama hingga kelahiran kedelapan pada sapi bali di Desa Galungan yaitu 3,6 ± 1,50 bulan sehingga tergolong terjadi keterlambatan munculnya estrus postpartum (anestrus postpartum).
Kadar Estrogen dan Munculnya Estrus setelah Pemberian Buserelin (Agonis GnRH) pada Sapi Bali yang Mengalami Anestrus Postpartum Akibat Hipofungsi Ovarium Ni Ketut Suartini; I Gusti Ngurah Bagus Trilaksana; Tjok Gde Oka Pemayun
Veterinary Science and Medicine Journal Vol 1 No 2 (2013)
Publisher : Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (141.489 KB)

Abstract

The purpose of this study was to measure the levels of estrogen and the onset of estrous after administration of Buserelin on bali cattle experiencing anestrous postpartum due to ovarian hypofunction. This research used twenty seven bali cattles which were divided evenly into three groups of treatment, namely group-one as control group (P0, without treatment), group-two, receiving a single injection of Buserelin at dose of 0.02 mg (P1) and group three, receiving repeated injection of Buserelin at dose of 0.02 mg at 24 hours interval (P3). Serum estrogen levels were measured at a time off before estrous, a time of estrous, and five days after the onset of estrous using ELISA method. Data were analyzed using statistical analysis of variance and a Duncan’s Multiple Range Test for a further analysis. The results showed that at the stage of estrous, the estrogen level of P0 was lower significantly (p<0.05) than two others treatments (P1 and P2), but those of P1 and P2 were statistically the same (p>0.05). The onset of estrous on P2 was shorter than those of P1 (p<0.05). It can be concluded that the administration of Buserelin can trigger an estrous on Bali cattle that experiencing anestrous postpartum due to ovarian hypofunction and repeated administrations of Buserelin can shorten the onset of estrous.
Peluang Penggunaan Spermatozoa Epididimis Yang Dikoleksi Setelah Kematian Sebagai Sumber Sel Gamet pada Anjing I Ketut Puja; IGN Trilaksana; Rudy Lontoh
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 10, No 2 (2005): June 2005
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v10i2.2846

Abstract

The purpose of this study was to determine the effect of postmortem time on percentage of life epididymal sperm from postmortem canine caudae epididymides. A total of 9 dog were used and divided into three group. T0 was control group, T1, 3 hours postmortem and T2, 6 hours postmortem. This way, samples were obtained at different times postmortem. Sperm were extracted from the caudae epididymis by means of cuts. The result showed that the percentage of life sperm were 67,16 ± 5.67(T0), 46.33± 5.60 (T1) and 24.00 ± 4.35 respectively. We could appreciate that percentage of life was affected by postmortem time. There was significant decrease life sperm recovered from epididymides postmortem (P<0.01). In conclusion, epididymal sperm from dog undergo decrease of percentage of life, but it could stay acceptable within many hours postmortem. We intepreted these data to indicate that it may still be possible to obtain viable spermatozoa many hours later.
REPRODUCTIVE EFFICIENCY OF SIMENTAL-BALI CROSSBRED CATTLE (SIMBAL) AND BALI CATTLE REARED IN CATTLE GROUPS IN PRINGGARATA SUBDISTRICT, CENTRAL LOMBOK Nurul Faiza; Tjok Gde Oka Pemayun; I Gusti Ngurah Bagus Trilaksana
Buletin Veteriner Udayana Bul. Vet. Udayana. October 2024 Vol. 16 No. 5
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2024.v16.i05.p06

Abstract

Reproductive efficiency is a measure of a cattle's ability to produce offspring. The objective of this study was to identify and compare the reproductive efficiency of Bali cattle and Simbal cattle raised in livestock groups in the Pringgarata Subdistrict. The sample size consisted of 198 cattle. Data were collected through interviews based on questionnaires. The data were analyzed using One Way ANOVA in SPSS version 22. The results of the study showed that the age at puberty for Bali cattle was 20,7±0,8 months and for Simbal cattle was 20,5±1,0 months. The average age at first mating for Bali cattle was 20,7±0,8 months, while for Simbal cattle it was 20.5±1.0 months. Estrous cycle for Bali cattle was 21,5±1,0 months, while for Simbal cattle it was 22,1±0,8 months. Service per conception was 2,0±0,9 for Bali cattle and 1,8±0,8 for Simbal cattle. The average age at first calving for Bali cattle was 29,7±1,4 months, and for Simbal cattle it was 29,6±1,4 months. The average postpartum estrus period for Bali cattle was 2,9±0,8 months, while for Simbal cattle it was 2,5±0,5 months. The calving interval for Bali cattle was 13,7±,0 months and 13,3±0,8 months for Simbal cattle. Based on these results, it can be concluded that the reproductive efficiency of Bali cattle and Simbal cattle in Pringgarata district shows no significant difference in age at puberty, age at first mating, and age at first calving. However, there were significant differences in the estrous cycle, service per conception, postpartum estrus, and calving interval, although the overall reproductive efficiency was still considered good.
PHYSIOLOGICAL AND BEHAVIOURAL DYNAMICS OF INDO-PACIFIC BOTTLENOSE DOLPHINS DURING THE PROCESS OF TRANSFER, ADAPTATION, AND ENVIRONMENTAL ENRICHMENT IN A NEW EX-SITU HABITAT Adnyana, Ida Bagus Nararya Primastana; Adnyana, Ida Bagus Windia; Trilaksana, I Gusti Ngurah Bagus; Suartha, I Nyoman; Pemayun, Tjok Gde Oka; Sudisma, I Gusti Ngurah
Buletin Veteriner Udayana Bul. Vet. Udayana. February 2026 Vol. 18 No. 1
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2026.v18.i01.p26

Abstract

This study aimed to evaluate the effects of ex-situ habitat transfer on physiological and behavioral stress responses in Indo-Pacific bottlenose dolphins (Tursiops aduncus) and to assess the effectiveness of environmental enrichment in mitigating stress and enhancing welfare. Seven dolphins were monitored across four periods: before transfer, immediately upon arrival at the new facility, after 15 days of adaptation, and following 30 days of environmental enrichment using interactive toys. Parameters measured included neutrophil-to-lymphocyte (N/L) ratio, respiratory rate, frequency of stereotypic behaviors, food intake, social interactions, and compliance with trainer commands. The results demonstrated that habitat transfer induced significant increases in N/L ratio and respiratory rate. A 15-day adaptation period was insufficient to restore physiological and behavioral parameters to baseline levels. However, after 30 days of environmental enrichment, stress indicators improved markedly, with decreased N/L ratios, normalized respiratory rates, reduced stereotypic behaviors, and increased food intake, social interactions, and compliance. These findings underscore the importance of integrating structured environmental enrichment strategies into ex-situ conservation and management programs to support optimal welfare in captive dolphins.
QUALITY OF BERKSHIRE BOAR SEMEN DILUTED WITH A COMBINATION OF BELTSVILLE THAWING SOLUTION AND MELATONIN AT DIFFERENT STORAGE TIMES Pradnyani, Gusti Ayu Putu Indira; Bebas, Wayan; Pemayun, Tjok Gde Oka; Trilaksana, I Gusti Ngurah Bagus; Laksmi, Desak Nyoman Dewi Indira; Susari, Ni Nyoman Werdi
Buletin Veteriner Udayana Bul. Vet. Udayana. February 2026 Vol. 18 No. 1
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2026.v18.i01.p29

Abstract

Berkshire pigs are a popular choice for small and large scale pig farming enterprises worldwide, including in Bali. Berkshire pigs are well known for their superior meat quality and relatively fast growth rate. This study aimed to determine the effect of storage duration on the quality of Berkshire boar spermatozoa diluted with Beltsville Thawing Solution (BTS®) supplemented with melatonin. Semen from Berkshire boars was diluted using BTS® supplemented with melatonin at a concentration of 1.0 mM and stored at a temperature of 15 –20°C. Semen quality was evaluated at different storage intervals: (P1) 48 hours, (P2) 60 hours, and (P3) 72 hours. The observed semen quality parameters included sperm motility, sperm abnormalities, sperm viability, and plasma membrane integrity of spermatozoa, which were examined using smear preparations observed under a microscope. In addition, sperm malondialdehyde (MDA) levels as an indicator of oxidative stress were measured using the Porcine MDA ELISA Kit. Duncan’s multiple range test analysis showed that the storage duration of Berkshire boar semen diluted with BTS® supplemented with melatonin was able to maintain sperm motility, abnormality, viability, plasma membrane integrity, and malondialdehyde levels up to 72 hours of storage, with sperm motility of 40.66%, sperm viability of 53.00%, and sperm abnormalities of 6.33%. These values met the minimum standards set by the Indonesian National Standard (SNI 8034:2023) for liquid boar semen. These results indicate that melatonin acts as an antioxidant capable of neutralizing free radicals, thereby inhibiting the occurrence of oxidative stress in spermatozoa cells.
Co-Authors Achmad Noerkhaerin Putra Adnyana, Ida Bagus Nararya Primastana AJI WAHYU WICAKSONO Apriliana, Kadek Soma Apriliani, Karolina Desak Nyoman Dewi Indira Laksmi Desak Nyoman Dewi Indira Laksmi DEVI INDRAWATI Drystiana Yessi Ayu Lesmono Dyah Risma Aprilia Enji Dyonesia Dimitri Maya Mayesta Ester Novitasari Fania, Bella Gede Putra Sanjaya Gunawan, I. W. N. F. Herdi Wahyu Adi Prananda Heros, Made Novi Lerianti I Gede Hendi Saputra I Gusti Ngurah Agung Dolly Octa I Gusti Ngurah Sudisma I Ketut Puja I Ketut Suatha I Ketut Suatha I Nyoman Oka Widiarta I Nyoman Oka Widiarta I Nyoman Suartha I Putu Agus Kertawirawan I Putu Sudana Agustina I Putu Suparman I Wayan Bebas I Wayan Gorda I WAYAN PUTRA ARIMBAWA I Wayan Sukernayasa Ida Bagus Windia Adnyana imam sobari Komang Gita Permana Kresensia Cyntia Dosom Kristyari, Ni Putu Gita Laba Mahaputra Laksmi, D. N. D. I Luh Made Sudimartini MADE KOTA BUDIASA Mahasanti, I Gusti Ayu Puji Ni Ketut Suartini Ni Komang Asni Ni Komang Sri Puspaningsih Ni Made Dwi Permata Sari Ni Nyoman Werdi Susari Ni Nyoman Werdi Susari Nurul Faiza Pradnyani, Gusti Ayu Putu Indira Priharyanthi, Luh Komang Ayu Puteri Rahel Situmorang Rinna Noviyati Ndun Rudy Lontoh Sandra, Arista Novi Saridewi, I Gusti Ayu Made Suputra, Gusde Wahyu Krisna Teja, Dewa Ngakan Gede Surya Tjok Gde Oka Pemayun Tjok Gde Oka Pemayun Tjok Gde Oka Pemayun Tjok Gde Oka Pemayun Tjok Gede Oka Pemayun Wayan Bebas Widiastuti, Wayan Arni Yudiani, Putu Mira