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All Journal Jurnal Ilmu dan Kesehatan Hewan Buletin Veteriner Udayana Jurnal Veteriner Indonesia Medicus Veterinus Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Journal of the Indonesian Tropical Animal Agriculture

I Gusti Ngurah Bagus Trilaksana

Laboratorium Reproduksi Veteriner Fakultas Kedokteran Hewan Universitas Udayana

Author-ID : 405705

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Earth & Planetary Sciences Health Professions Immunology & microbiology Medicine & Pharmacology Public Health Veterinary

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Waktu Munculnya Birahi Pascamelahirkan pada Sapi Bali di Desa Galungan, Sawan, Buleleng, Bali Yudiani, Putu Mira; Trilaksana, I Gusti Ngurah Bagus; Laksmi, Desak Nyoman Dewi Indira
Indonesia Medicus Veterinus Vol 10 (6) 2021
Publisher : Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19087/imv.2021.10.6.896

Penelitian ini bertujuan untuk mengetahui waktu munculnya birahi pascamelahirkan/estrus postpartum pada sapi bali yang dipelihara di Desa Galungan. Penelitian ini dilaksanakan dari bulan Desember 2020 hingga Februari 2021 di Desa Galungan, Kecamatan Sawan, Buleleng, Bali. Penelitian ini menggunakan 91 ekor sapi betina (primipara dan pluripara). Data yang didapat kemudian ditabulasikan dan dianalisis secara deskriptif. Hasil analisis didapat bahwa manajemen kandang oleh peternak sapi bali di Desa Galungan yaitu kandang tunggal sebanyak 93,38%, kandang terbuka 69,42%, dan tidak adanya pejantan yaitu 52,06%. Rata-rata estrus postpartum kelahiran pertama yaitu 3,8 ± 1,55 bulan, kelahiran kedua yaitu 3,52 ± 1,48 bulan, kelahiran ketiga yaitu 3,37 ± 1,46 bulan, kelahiran keempat yaitu 3,36 ± 1,42 bulan, kelahiran kelima yaitu 3,35 ± 1,32 bulan, kelahiran keenam yaitu 3,42 ± 1,07 bulan, kelahiran ketujuh yaitu 3,75 ± 1,32 bulan, dan kelahiran kedelapan yaitu 4,33 ± 1,67 bulan. Kelahiran kedua hingga kelima pada estrus postpartum lebih cepat dibandingkan kelahiran pertama, dan estrus postpartum keenam hingga kedelapan mulai lambat. Komposisi pakan yang diberikan oleh peternak ke sapi-sapi mereka yaitu 50% rumput gajah, 19% rumput sertaria, dan 15% daun gamal. Sebagian besar peternak hanya mengandalkan pakan hijauan dan tidak menggunakan pakan tambahan. Rata-rata estrus postpartum kelahiran pertama hingga kelahiran kedelapan pada sapi bali di Desa Galungan yaitu 3,6 ± 1,50 bulan sehingga tergolong terjadi keterlambatan munculnya estrus postpartum (anestrus postpartum).

Kadar Estrogen dan Munculnya Estrus setelah Pemberian Buserelin (Agonis GnRH) pada Sapi Bali yang Mengalami Anestrus Postpartum Akibat Hipofungsi Ovarium Ni Ketut Suartini; I Gusti Ngurah Bagus Trilaksana; Tjok Gde Oka Pemayun
Veterinary Science and Medicine Journal Vol 1 No 2 (2013)
Publisher : Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (141.489 KB)

The purpose of this study was to measure the levels of estrogen and the onset of estrous after administration of Buserelin on bali cattle experiencing anestrous postpartum due to ovarian hypofunction. This research used twenty seven bali cattles which were divided evenly into three groups of treatment, namely group-one as control group (P0, without treatment), group-two, receiving a single injection of Buserelin at dose of 0.02 mg (P1) and group three, receiving repeated injection of Buserelin at dose of 0.02 mg at 24 hours interval (P3). Serum estrogen levels were measured at a time off before estrous, a time of estrous, and five days after the onset of estrous using ELISA method. Data were analyzed using statistical analysis of variance and a Duncan’s Multiple Range Test for a further analysis. The results showed that at the stage of estrous, the estrogen level of P0 was lower significantly (p<0.05) than two others treatments (P1 and P2), but those of P1 and P2 were statistically the same (p>0.05). The onset of estrous on P2 was shorter than those of P1 (p<0.05). It can be concluded that the administration of Buserelin can trigger an estrous on Bali cattle that experiencing anestrous postpartum due to ovarian hypofunction and repeated administrations of Buserelin can shorten the onset of estrous.

Pengaruh Frekuensi Penampungan Semen Terhadap Konsentrasi Dan Abnormalitas Spermatozoa Burung Puyuh Wayan Bebas; Komang Gita Permana; I Gusti Ngurah Bagus Trilaksana; Desak Nyoman Dewi Indira Laksmi
Buletin Veteriner Udayana Vol. 10 No. 2 Agustus 2018
Publisher : The Faculty of Veterinary Medicine, Udayana University

The aims of this study are to determine the influence of collecting frequency against concentration and spermatozoa abnormalities of quail. This study used a completely randomized design (CRD) with 3 treatment groups, is collecting sement at 2, 3 and 4 days intervals. Each treatment is repeated 9 times. Observations were made on the concentration of spermatozoa (107 cells/ml) and abnormalities of spermatozoa (%). The data were analyzed using analysis of variance (ANOVA) if there are significantly different (P<0.05) followed by Duncan test. The results showed the concentration of spermatozoa in each treatment respectively 39.00±5.148 x107 cells/ml; 59.49±1.878 x107 cells/ml; 59.11±2.028 x107 cells/ml. Abnormality in each treatment was 14.22±1.394%; 7.22±1.202%; 7.49±1.130%. The frequency of ejaculation was statistically significant (P<0.05) against the concentration and spermatozoa abnormalities of quail.

Kadar Hormon Estrogen pada Sapi Bali saat Pubertas Herdi Wahyu Adi Prananda; Desak Nyoman Dewi Indira Laksmi; I Gusti Ngurah Bagus Trilaksana
Buletin Veteriner Udayana Vol. 14 No. 3 June 2022
Publisher : The Faculty of Veterinary Medicine, Udayana University

Bali cattle is one of the most important native cattle in Indonesia. There is one measure of reproductive efficiency. The achievement of early age of puberty in accordance with its genetic potential is important to achieve optimum reproductive performance and provide increase in productivity. One of the things that affects the appearance of puberty is the amount of estrogen in the blood serum. The purpose of this study was to determine the levels of estrogen in cattle blood serum. The study was conducted by recording the age first and then taking blood in cattle that had signs of puberty and then carried out checking hormone levels using the ELISA method. The results showed that the average age of puberty was 21.44 months and the average estrogen level at puberty was 84.75 ng / ml. Testing with correlation and regression tests showed a close relationship between age and estrogen levels at puberty with a correlation coefficient of r = 0.798.

Intensitas Estrus Sapi Bali yang Mengalami Silent Heat I Putu Sudana Agustina; Desak Nyoman Dewi Indira Laksmi; I Gusti Ngurah Bagus Trilaksana; Made Kota Budiasa
Buletin Veteriner Udayana Vol. 13 No. 2 Agustus 2021
Publisher : The Faculty of Veterinary Medicine, Udayana University

This research was conducted to determine the follicle diameter and estrous intensity of bali cattle that ever had a silent heat in the livestock group of Integrated Farming Systems (SIPADU) in Mengwi Subdistrict Badung Regency, Bali Province, which was implemented in September – Oktober 2019. Bali cattle are grouped into 2 (two) groups; first, the Bali cattle, whichever had silent heat. Second, the Bali cattle whichever had estrous. The results showed the estrous intensity of bali cattle with silent heat showed significant differences (p < 0.05), it is vulva temperature (37,68 + 0,32ºC), vulva diameter (27.64 + 4.28 mm), vulva color (pink, invisible peripheral vessels ) and vulva mucus (no mucus until there was little mucus ). Compared to the group of bali cattle which had estrus, it is vulva temperature (37.96 + 0.51°C), vulva diameter (31,19 + 5,66 mm), vulva color (bright red to dark red and peripheral vessels branching clearly) and vulva mucus (viscous, transparent hang out). The results of the study concluded that there was a difference between the estrus intensity in bali cattle that had silent heat and bali cattle that showed true estrus.

Faktor-Faktor yang Mempengaruhi Tidak Tercapainya Target Pelaksanaan Inseminasi Buatan pada Upsus Siwab di Kabupaten Jembrana Ni Komang Sri Puspaningsih; Gusti Ngurah Bagus Trilaksana; I Ketut Puja
Buletin Veteriner Udayana Vol. 14 No. 2 April 2022
Publisher : The Faculty of Veterinary Medicine, Udayana University

Bali cattle are a type of beef cattle that are widely developed in Indonesia because of their superiority. UPSUS SIWAB is an effort to accelerate the increase of livestock populations by intensifying the breeding of female cattle by utilizing artificial insemination technology. Based on the artificial insemination’s rate of success, Jembrana Regency has 31.87% the success rate of artificial insemination which is not optimal. This study aimed to determine the factors that influence the failure to achieve the target of artificial insemination at UPSUS SIWAB in Jembrana Regency. The method that used to collecting data in this study is by using a questionnaire and analyzed by analysis of factors. The results of this study are used the breeding variables, always called the officiers, and knowing the benefit of artificial insemination is main factors that influence the failure to achieve the target of artificial insemination in Jembrana Regency. Counseling need to be made about the benefit of artificial insemination so that more breeders can accept the existence of this technology and they can achieve the target of implementing artificial insemination in Jembrana Regency specially.

Perkembangan Folikel dan Munculnya Estrus setelah Penyuntikan GnRH pada Sapi Bali yang Mengalami Anestrus Postpartum dengan Body Condition Score Berbeda I Nyoman Oka Widiarta; Tjok Gde Oka Pemayun; I Gusti Ngurah Bagus Trilaksana
Buletin Veteriner Udayana Vol. 12 No. 1 Pebruari 2020
Publisher : The Faculty of Veterinary Medicine, Udayana University

This study aim to determine the development of follicles and onset of estrus due to GnRH induction in bali cattle which has anestrus postpartum more than three month with differnt body condition score in the Badung regency of Bali.The designd used in this study was a complete random design were divided into three treatment groups, namely Group 1 : cattle with BCS 2, Group 2 : cattle with BCS 3, Group 3 : cattle with BCS 4, each group consisted of 9 replication. All of group of bali cattle are induced by GnRH (Fertagyl, Intervet,Inc) at a dose of 500µg/IM/cattle. The result of this study indicate the development of follicles averages before induction of GnRH is 4.03 + 0.21 mm, 4.60 + 0.18 mm, 4.56 + 0.22 mm, each for BCS2, BCS 3 and BCS 4 and development of follicles averages after induction of GnRH is 7.08 + 0.42mm, 11.06 + 0.40 mm and 11.99 + 0.33 each for BCS 2, BCS 3 and BCS 4 and statistically shows that there are significant differences (P<0,05). The averages onset of estrus in the three treatment group is 7.75 + 0.89 day, 4.63 + 0.52 day dan 3.63 + 0.52day, each for BCS 2, BCS 3 and BCS 4 and statistically shows that there are significant differences (P<0,05). The largest follicular diameter in BCS 4 is 11,99 + 0,33 mm with the time of onset of estrus 3,63 + 0,52 day. The result can be concluded that the induction of GnRH 500ug in bali cattle can increase follicular development and accelerate the oset of estrous.

Efektivitas Prostaglandin F2? dalam Menginduksi Berahi, Non Return Rate dan Conception Rate pada Sapi Bali Anestrus Postpartum I Putu Agus Kertawirawan; I Gusti Ngurah Bagus Trilaksana; Tjok Gde Oka Pemayun
Buletin Veteriner Udayana Vol. 13 No. 2 Agustus 2021
Publisher : The Faculty of Veterinary Medicine, Udayana University

Induction of heat is one of the technologies in the field of reproduction to accelerate the appearance of heat in cattle. Many methods are used to induction of heat, one of them is by giving prostaglandin F2?. This study aims to determine the effectiveness of the administration of PGF2? in bali cattle anestrus postpartum with corpus luteum functioning and different body weights to induce the appearance of heat, non-return rate, and conception rate after insemination once and twice at 12-hour intervals. This research was conducted using a randomized block design based on body weight and the number of insemination. Group I (P1) is a group with a bodyweight of less than 250 kg and received one insemination when the appearance of lust, Group II (P2) with a bodyweight of less than 250 kg and received two inseminations at 12-hour intervals, group III (P3) with body weights above 250 kg with one insemination and group IV (P4) with body weights above 250 kg and received twice insemination at 12-hour intervals. Statistical analysis and testing are done by the analysis of variance. The results showed that the administration of PGF2? caused the emergence of estrus in all experimental animals and there was no significant difference (P> 0.05) to the appearance of heat between the four treatment groups, and the insemination treatment twice intervals 12 hours also had no significant effect to enhance the number of non-return rate and conception rate.

Madu Meningkatkan Kualitas Semen Kalkun Selama Penyimpanan (HONEY IMPROVE THE QUALITY OF SEMEN TURKEY DURING STORAGE) Ni Made Dwi Permata Sari; Wayan Bebas; I Gusti Ngurah Bagus Trilaksana
Buletin Veteriner Udayana Vol. 7 No. 2 Agustus 2015
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (144.157 KB)

This study aims were to determine the addition of various concentrations of honeyon themotility and viability of spermatozoa turkeywere diluted with phosphate yolk stored at 5°C for 72hours. This study used a completely randomized design factorial pattern. Four treatment groups,respectively: without the addition of honey (control), the addition of 3% honey, honey 4%, and 5% honey. A total of seven factors of each storage time: storage for 0 h, 12 h, 24 h, 36 h, 48 h, 60 h and72 h. Each treatment be repeated six times. Semen quality was observed in the form of examinationof progressive motility and viability of spermatozoa examination. The data obtained was examinedby the General Linear Model (Multivariate). The results showed that the addition of honey givessignificant differences on the progressive motility and viability of spermatozoa during storage. Itcan be concluded that the addition of 3% honey was the best dose to maintain motility and viabilityof spermatozoa turkeys during storage at 5°C temperature.

Penurunan Kadar Progesteron Setelah Pemberian PGF 2? Yang Diekstrak Dari Sel Monolayer Vesikula Seminalis Sapi Bali Disimpan Pada Suhu Dan Waktu Berbeda (DECREASE LEVEL OF PROGESTERONE AFTER TREATED WITH PGF 2? EXTRACTED FROM BALI CATTLE VESICULA SEMINAL Tjok Gde Oka Pemayun; I Gusti Ngurah Bagus Trilaksana; Laba Mahaputra
Buletin Veteriner Udayana Vol. 7 No. 1 Pebruari 2015
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (157.346 KB)

The aim of this study was to evaluate the efectiveness of PGF2? on the decrease level of progesterone after treated with PGF2? that extracted from bali cattle vesicula seminalis monolayercell that stored for 0 month, two months and four months at temperature 40C and -4oC. Sample that used in this study was PGF2? producted from bali cattle vesicula seminalis monolayer cell. Acomplete random sample with two factorial pattern was used i.e; factor I was the period stored (0month, two months, and four months) and factor II was the temperature storage (4oC).Each treatment was completely with repetitions. Result showed that PGF2? that resulted from balicattle monolayer cell vesikula seminalis that stored for two months was able to decrease level ofprogesterone to 49.06% at -4oC and 48.82% at temperature 40C. Similarly. The PGF2? that storedfor four months affected on the decrease of progesterone level to 36.33% at temperature -4C and35.81% at temperature 40C. It can be concluded that PGF2? that resulted in the study that stored forfour months at temperature of -40C and 40C may have capability to regress luteal cell.

Co-Authors Achmad Noerkhaerin Putra AJI WAHYU WICAKSONO Apriliana, Kadek Soma Apriliani, Karolina Desak Nyoman Dewi Indira Laksmi Desak Nyoman Dewi Indira Laksmi DEVI INDRAWATI Drystiana Yessi Ayu Lesmono Dyah Risma Aprilia Enji Dyonesia Dimitri Maya Mayesta Ester Novitasari Fania, Bella Gede Putra Sanjaya Gunawan, I. W. N. F. Herdi Wahyu Adi Prananda Heros, Made Novi Lerianti I Gede Hendi Saputra I Gusti Ngurah Agung Dolly Octa I Ketut Puja I Ketut Suatha I Ketut Suatha I Nyoman Oka Widiarta I Nyoman Oka Widiarta I Putu Agus Kertawirawan I Putu Sudana Agustina I Putu Suparman I Wayan Bebas I Wayan Gorda I WAYAN PUTRA ARIMBAWA I Wayan Sukernayasa imam sobari Komang Gita Permana Kresensia Cyntia Dosom Kristyari, Ni Putu Gita Laba Mahaputra Laksmi, D. N. D. I Luh Made Sudimartini MADE KOTA BUDIASA Mahasanti, I Gusti Ayu Puji Ni Ketut Suartini Ni Komang Asni Ni Komang Sri Puspaningsih Ni Made Dwi Permata Sari Ni Nyoman Werdi Susari Priharyanthi, Luh Komang Ayu Puteri Rahel Situmorang Rinna Noviyati Ndun Rudy Lontoh Sandra, Arista Novi Saridewi, I Gusti Ayu Made Suputra, Gusde Wahyu Krisna Teja, Dewa Ngakan Gede Surya Tjok Gde Oka Pemayun Tjok Gde Oka Pemayun Tjok Gde Oka Pemayun Tjok Gede Oka Pemayun Wayan Bebas Widiastuti, Wayan Arni Yudiani, Putu Mira

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