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All Journal AL KAUNIYAH El-Hayah : Jurnal Biologi SAINSTIS Padjadjaran Journal of Dentistry Jurnal Biodjati Jurnal Pengabdian Pada Masyarakat Padjadjaran Journal of Dental Researchers and Students INDONESIAN JOURNAL OF MEDICAL LABORATORY SCIENCE AND TECHNOLOGY Jurnal Riset Biologi dan Aplikasinya Jurnal Abdimas Bina Bangsa Jurnal Kesehatan Gigi dan Mulut (JKGM) Jurnal Terapi Gigi dan Mulut Media Penelitian dan Pengembangan Kesehatan
Ulfah Utami
Universitas Islam Negeri Maulana Malik Ibrahim Malang
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Decision Sciences, Operations Research & Management Dentistry Earth & Planetary Sciences Education Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Nursing Public Health Social Sciences Other

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Journal : INDONESIAN JOURNAL OF MEDICAL LABORATORY SCIENCE AND TECHNOLOGY

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Analysis of Exopolysaccharides in Lactobacillus casei group Probiotics from Human Breast Milk Nur Kusmiyati; Yuni Puspitasari; Ulfah Utami; Anggeria Oktavisa Denta
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 5 No 1 (2023): Step up to strengthen the laboratory system and prepare for patients care
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/ijmlst.v5i1.2872

Abstract

Exopolysaccharides get a lot of attention because they can improve the host immune system. Exopolysaccharide is a polysaccharide that is produced and secreted from microbes outside the cell, usually found on the outside of the bacterial structure. The Lactobacillus casei group from human breast milk is thought to have the ability to produce exopolysaccharides. The purpose of the study was to examine the exopolysaccharide of the L. casei group that was isolated from breast milk. The methods used include the gravimetric, the phenol-sulfuric acid and the Fourier Transform Infra-Red (FTIR). The results showed that the L. casei group could produce exopolysaccharides, and had high exopolysaccharide total sugar content. Lactobacillus paracasei had the highest exopolysaccharide and total sugar content of 3660 mg/L and 80.6%, respectively. The FTIR results of the L. casei group exopolysaccharides showed the presence of hydroxyl functional groups O-H (3425.76-3295.98 cm-1), methyl C-H (2930.86-2856.70 cm-1), carbonyl C=O (1660.11-1647.27 cm-1), C-H (1456.16-1373.44 cm-1) and C-O-C ether (1071.08-1056.82 cm-1) which are specific characters of exopolysaccharides. Since the FTIR profile demonstrates that the L. casei group can produce exopolysaccharides, it has greater potential as a a probiotic.
Co-Authors Afifah, Zahroul Ahdiansyah, Balqis Shafa' Ahmad Barizi Anggeria Oktavisa Denta Aprilianty, Windy Marsella Arifin, Naufal Aqila Azizah, Dita Nur Azizatur Rahmah Chaerudin, Denden Ridwan Dina Istia'nah ELDARITA, ELDARITA Elfiyatinnufus, Rifqiyah Fitriyah Fitriyah Fitriyani, Annisa Fuzzi Heriyanto, Yonan Husna, Naila Fatihatul Istia'nah, Dina Izzatinnisa' Izzatinnisa' Izzatinnisa', Izzatinnisa' Kamila, Syadza Afifah Laela, Dewi Sodja Laut, Deru Marah Liliek Harianie Lilik Hariani Marah Laut, Deru Megananda Hiranya Putri Muh Amin, dwi aisyah Muhammad Asmuni Hasyim Muhammad Riefki Pratama Mujahidin Ahmad Neneng Nurjanah Nur Kusmiyati Nurnaningsih, Hera Nurul Fatikhah, Nurul Nuzulul Zahroh Praptiwi, Yenni Hendriani Prilya Dewi Fitriasari Retno Setyaningrum Sari, Cici Rafita Siti Fatimah Sri Mulyanti Sulistyantini, Cahyaning Supriyanto, Irwan Widyastuti , Tri Yuni Puspitasari Yuti Malinda