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The Chemical Composition of Gracilaria verrucosa Extract and its Utilization on Survival and Growth Litopenaeus vannamei Jasmanindar, Yudiana; Sukenda, Sukenda; Alimuddin, Alimuddin; Junior, Muhammad Zairin; Utomo, Nur Bambang Priyo
Journal Omni-Akuatika Vol 14, No 3 (2018): Omni-Akuatika November
Publisher : Fisheries and Marine Science Faculty - Jenderal Soedirman University

The Gracilaria genus is a potential source of natural and environmentally-friendly alternatives in improving the survival and growth of shrimp. This study aims to identification immunostimulant molecules extract G. verrucosa and evaluate the utilization of G verrucosa extract as an immunostimulant in improving survival and growth of L. vannamei. Seaweed extraction used ethyl acetate then formulated in the diets. The immunostimulant molecule in the G. verrucosa was analysis. The shrimp were fed a test diet containing extract G. verrucosa at a dose of 2 g kg-1 or extract G. verrucosa-free control diets for 42 days. Shrimps were fed diets containing extract with a specific duration. The observation on the survival and growth of L. vannamei was performed after maintenance at the Laboratory for six weeks. Following, diets containing extract was tested in the field (pond shrimp farm) at the same dose of extract for 58 days. Shrimp was feed diets containing extract once a week, once in the early culture, and diet control, then the survival and growth shrimp were analysis. Concentrations of sulfates and carbohydrates in G. verrucosa ethyl acetate-extract were 24.21% and 13.41%, and crude protein 3.64%. GC-MS pyrolysis results show that G. verrucosa polysaccharide is similar to immunostimulant molecules. The survival shrimp gave diets containing G. verrucosa extract formulation was higher than that of shrimps fed controls diet. The Shrimp fed diets extracts have higher growth than shrimp given control dietsKeywords: Gracilaria, extract, polysaccharides, immunostimulant

Masculinization of Nile tilapia (Oreochromis niloticus) by administration of bull testes meal Muslim, Muslim; Junior, M. Zairin; Utomo, Nur Bambang Priyo
Jurnal Akuakultur Indonesia Vol. 10 No. 1 (2011): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

The synthetic steroid 17α-Methyltestosteron (MT) is commonly used as a feed additive to produce male population of Nile tilapia (Oreochromis niloticus). The use of synthetic testosterone hormone is not recommended in Indonesia. This study was conducted to evaluate the effect of natural testosterone hormone in bull testes meal (BTM) on the masculinization of Nile tilapia using validated aceto carmine squash method of gonads of the fish. Experimental design was utilized two factors experiments in completely randomized design. Fry kept in 40-L glass aquaria at a density of 40 fry/aquarium. Fry (7 dph) received the BTM for 7 days (T1), 14 days (T2) and 21 days (T3) and doses 0% (D1), 3% (D2), 6% (D3), and 9% (D4). When treatment was these results, indicated that significant (P≥0.05) masculinization occurred only in the group treated of BTM and no treated of BTM. In the group treated of BTM, doses and duration treatment is not significant. The percentage of male fish 83.3% (9%-7d, 9%-21d, 6%-21d: doses and duration, respectively), higher than all group. Survival rate of fry (95-99.5%) is not affected by treatment BTM (no significant P≥0.05). Fish growth was significantly affected by treatment BTM compare with no treated of BTM. The highest growth performance of fry were obtained with the 9% BTM.Key words: masculinization, nile tilapia, bull testes meal ABSTRAKSteroid sintetik 17α-Methyltestosteron (MT) umumnya digunakan sebagai aditif pakan untuk menghasilkan populasi ikan nila jantan (Oreochromis niloticus). Penggunaan hormon testosteron sintetis tidak dianjurkan di Indonesia. Penelitian ini dilakukan untuk mengevaluasi pengaruh hormon testosteron alami dalam makanan testis banteng (BTM) pada maskulinisasi ikan nila menggunakan metode divalidasi aceto carmine squash, dari gonad ikan. Desain eksperimental dimanfaatkan dua eksperimen faktor dalam desain benar-benar acak. Fry disimpan dalam 40-L akuarium kaca pada kepadatan 40 fry/akuarium. Fry (7 DPH) menerima BTM selama 7 hari (T1), 14 hari (T2) dan 21 hari (T3) dan dosis 0% (D1), 3% (D2), 6% (D3), dan 9% (D4). Ketika pengobatan hasil ini, menunjukkan signifikan (P ≥ 0,05) hanya terjadi maskulinisasi pada kelompok perlakuan dari BTM dan tidak diperlakukan BTM. Pada kelompok diobati BTM, dosis dan durasi pengobatan tidak signifikan. Persentase ikan jantan 83,3% (9%-7d, 9%-21d, 6%-21d: dosis dan durasi, masing-masing), lebih tinggi dari kelompok semua. Tingkat kelangsungan hidup benih (95-99,5%) tidak dipengaruhi oleh pengobatan BTM (tidak ada P yang signifikan ≥ 0,05). Pertumbuhan ikan secara signifikan dipengaruhi oleh BTM pengobatan dibandingkan dengan tidak diobati BTM. Kinerja pertumbuhan tertinggi fry diperoleh dengan BTM 9%.Kata kunci: maskulinisasi, ikan nila, tepung testis sapi

The use of Spirulina platensis as tilapiaOreochromis niloticusdiet supplementation Utomo, Nur Bambang Priyo; Rahmatia, Firsty; Setiawati, Mia
Jurnal Akuakultur Indonesia Vol. 11 No. 1 (2012): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

Spirulina is blue green algae that have high nutrient content like protein and vitamin so itcan beused as source ofsingle cell protein (SCP). This research was conductedto know minimum doses of Spirulina in different protein feed on growth performance of tilapia. Experimental diets devided by two different protein level (25% of protein and 28% of protein) and three levels supplementation of Spirulina 0% (25A), protein level 25% supplementedSpirulina 3% (25B), protein level 25% supplementedSpirulina 6% (25C), protein level 28% supplementedSpirulina 0% (28A), protein level 28% suplemented Spirulina 3% (28B), and protein level 28% supplementedSpirulina 6% (28C). Testeds feedwere tilapia with average initial weight 17,24±0,29 g and density five fish/aquarium. Tilapia rearedin aquarium sized 50×40×35 cm3 filledwith 30 cm waterand provided altrecirculation system. Fish were cultured for 40 days and fed at satiation with three times feeding frequency. All data analyzed statistically by one-way ANOVA.Result demonstrated that various dosesof Spirulina in different protein level of feed influencedspecific growth rate, feeding efficiency, protein deposition, and fat deposition. Fish were fed by feed containing Spirulina 3% in protein level 25% showed growth performance as same as fed of protein level 28%. Keywords: feed protein, Spirulina, growth performance, tilapia

Growth, feed conversion and retention, and proximate of eel juvenile treated by immersion of recombinant giant grouper growth hormone Handoyo, Boyun; Alimuddin, .; Utomo, Nur Bambang Priyo
Jurnal Akuakultur Indonesia Vol. 11 No. 2 (2012): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

This study was aimed to determine the immersion dose of recombinant giant grouper growth hormone (rElGH) to increase growth of eel juvenile (glass eel). After shock salinity treatment (NaCl 3% for 2 min), glass eel were immersed in water containing 0.9% NaCl, 0.01% bovine serum albumin, and different of dose of rElGH (0, 0.12, 1.2, 12 and 120 mg/L). Glass eel were fed on blood worm for the first month, and commercial diet for the second month of rearing. Fish rearing was performed in 60 L glass aquarium at density of 150 fish per aquarium. The results showed that higher in growth body weight was obtained in immersion dose of 12 mg/L, by increment of about 37.4% higher compared to that of control. The immersion dose of 12 mg/L also increased specific growth rate by 29.3% higher (p<0.05) compared to control. Furthermore, at that immersion treatment, feed conversion ratio of blood worm and commercial diet decreased by 33.7% and 25.6% compared to control, respectively. Protein (7.15±0.08%) and lipid (9.95±0.10%) retentions in 12 mg/L rElGH-treated fish were higher (p<0.05) than those of control (protein 6.17±0.07%; lipid 5.73±0.06%). Lower in protein content (12.73%), while higher content in lipid (8.35%) and crude carbohydrate (3.22%) were found in 12 mg/L rElGH-treated fish compared to those of control (13.24%, 5.90%, and 1.76%, respectively). Thus, in general, rElGH immersion dose of 12 mg/L could generate high performances of eel juvenile, and application of rElGH can be useful to increase aquaculture eel production.Keywords: immersion dose, growth hormone, growth, glass eel

Growth and viability of juvenile humpback grouper (Cromileptes altivelis) supplemented with inorganic and organic selenium Hamzah, Muhaimin; Suprayudi, Muhammad Agus; Utomo, Nur Bambang Priyo; Manalu, Wasmen
Jurnal Akuakultur Indonesia Vol. 11 No. 2 (2012): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

This study was conducted to determine the effects of inorganic selenium (sodium selenite) and organic selenium (selenomethionine) supplementation on growth and viability of juvenile humpback grouper (Cromileptes altivelis). The experiment was designed as a completely randomized design with eight treatments and three replications. The treatment being tested was source and dosages of selenium ie., inorganic selenium (sodium selenite) supplementation with dosages of 0.5, 1, 2, and 4 mg Se/kg diet and organic selenium (selenomethionine) with dosages of 1, 2, and 4 mg Se/kg diet. Another treatment was unsupplemented selenium. Juveniles humpback grouper at an initial average length of 6,39±0,41 cm and body weight of 4,49±0,65 g were reared in 90×40×35 cm3 aquaria and fed artificial diet (pellet) two times daily (08.00 and 16.00) at satiation. Fishes were reared for 40 days at a stocking density of 12 fish per aquarium on sea water with salinity of 30–31 ppt and temperature of 28–29 °C. The results of this study showed that the source of selenium supplementation (inorganic or organic) affected growth performance, glutathione peroxidase (GPx) enzyme activity, and blood profiles of the experimental fish. Generally, it was found that selenomethionine supplementation resulted in better fish performance than sodium selenite. In sodium selenite supplementation, survival declined with the increased dosages of Se in the diet, and the supplementation at dosage of 0,5 mg Se/kg diet showed a toxic effects. Histopatological test showed that there were damage in livers, kidneys, and intestines of fish supplemented with sodium selenite from 0.5 to 4 mg Se/kg diet. On the contrary, supplementation of selenomethionine up to 4 mg Se/kg did not show any signs of toxicity and the survival was on 86.11 to 97.22%. Feed efficiency, protein retention, lipid retention, and Se retention indicated that a dosage of 4 mg Se/kg selenomethionine supplementation was the best dosage.Keywords: selenium, viability, growth, Cromileptes altivelis, humpback grouper

Fermentation of Azolla sp. leaves and the utilization as a feed ingredient of tilapia Oreochromis sp. Utomo, Nur Bambang Priyo; ., Nurfadhilah; Ekasari, Julie
Jurnal Akuakultur Indonesia Vol. 10 No. 2 (2011): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

ABSTRACTThe objective of this study was to study the effect of incubation period on the nutritional content of Azolla sp. meal fermented by Trichoderma harzianum, and its optimum supplementation level in the feed of tilapia Oreochromis sp. In incubation period treatments, fermentation of Azolla meal was performed in two, six, eight, and ten days (AF2, AF6, AF8, AF10) using Trichoderma harzianum as the fermentor. The fish used in this study was tilapia Oreochromis sp. with an average weight of 10.59±1.29 g. The design of the feeding treatments was repeletting commercial feed with Azolla leaves by with different supplementation levels, i.e. 0% (A/control), 30% (B), 60% (C), and 90% (D). Faecal collection for digestibility measurement was conducted for 15 days and fish growth rate was observed for 40 days. Azolla meal fermented for two days (AF2) showed the best results among the other treatments with a crude fiber decrease of 37.19% and protein increase of 38.65%. The results of this study indicate that fermentation can increase the nutritional quality of Azolla meal and its most optimal supplementation level in the diet of tilapia is 30%.Keywords: crude fiber, Azolla sp., tilapiaABSTRAKPenelitian ini bertujuan untuk mengetahui pengaruh lama waktu fermentasi daun mata lele Azolla sp. menggunakan kapang Trichoderma harzianum serta dosis optimal dalam pakan ikan nila Oreochromis sp. Proses fermentasi tepung daun mata lele dilakukan selama dua, enam, delapan, dan sepuluh hari (AF2, AF6, AF8, AF10). Ikan uji pada penelitian ini menggunakan ikan nila Oreochromis sp. dengan bobot rata-rata 10,59±1,29 g yang ditebar sebanyak 6 ekor/akuarium berukuran 50×45×30 cm3. Sebagai pakan perlakuan yakni repeletting daun mata lele dengan pakan komersil pada tingkat suplementasi 0% (A/kontrol), 30% (B), 60% (C), dan 90% (D). Pemeliharaan ikan uji dan pengumpulan data dilakukan dengan mengumpulkan feses ikan untuk uji ketercernaan selama 15 hari dan mengamati pertumbuhan ikan selama 40 hari. Tepung daun mata lele yang difermentasi selama dua hari (AF2) memiliki hasil yang paling baik di antara perlakuan lainnya yakni dengan penurunan serat kasar sebesar 37,19% dan peningkatan protein sebesar 38,65%. Hasil penelitian ini menunjukkan bahwa fermentasi dapat meningkatkan kualitas nutrisi daun mata lele serta dosis optimal pemanfaatan tepung daun mata lele fermentasi dalam pakan ikan nila adalah sebesar 30%.Kata kunci: serat kasar, Azolla sp., ikan nila

Role of various fishmeal ingredients on sangkuriang catfish Clarias sp. growth Utomo, Nur Bambang Priyo; Susan, ,; Setiawati, Mia
Jurnal Akuakultur Indonesia Vol. 12 No. 2 (2013): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

ABSTRACT This research was aimed to evaluate the role of fish meal from different raw materials in improving growth performance of sangkuriang catfish Clarias sp. The source of raw materials used were derived from trash fish, salted fish, and head fish eal. Fish reared for 44 days and were given feed test three times per day at 08.00, 12.00, and 16.00. Biomass weight of the experimental fish used were 38.49±1.61 g. The fish reared on the aquarium of 60x50x40 cm3 and by using recirculation system. The results showed that fish meal derived from trash fish provide the best result for feed consumption, daily growth rate, absolute length, biomass, retention of protein, retention of fat, feed efficiency, feed conversion (P>0.05). However, it does not significantly different on the survival of fish (P>0.05). The data showed that the use of trash fish meal resulted on the best growth performance of sangkuriang catfish. It was indicated that the trash-fish meal might be used as the main protein source of sangkuriang catfish feed. Keywords: catfish, growth, at satiation, fish meal ABSTRAK Penelitian ini bertujuan untuk mengevaluasi pakan uji yang menggunakan sumber tepung ikan dari bahan baku berbeda dalam meningkatkan kinerja pertumbuhan lele sangkuriang Clarias sp. Sumber tepung ikan yang digunakan berasal dari ikan rucah, ikan asin, dan kepala ikan, yang memiliki umum digunakan sebagai salah satu bahan baku pakan lokal. Ikan diberi pakan at satiation selama 44 hari dengan frekuensi tiga kali sehari, yaitu pada pukul 08.00, 12.00, dan 16.00. Biomassa rata-rata ikan uji yang digunakan adalah 38,49±1,61 g. Ikan dipelihara dalam akuarium berukuran 60x50x40 cm3 dan dengan menerapkan sistem resirkulasi. Hasil penelitian menunjukkan bahwa tepung ikan yang berasal dari ikan rucah mampu memberikan hasil terbaik dalam meningkatkan kinerja pertumbuhan ikan uji. Hal ini ditunjukkan oleh nilai kinerja pertumbuhan seperti jumlah konsumsi pakan, laju pertumbuhan harian, panjang mutlak, biomassa, retensi protein, retensi lemak, efisiensi pemberian pakan, dan konversi pakan yang menunjukkan perbedaan nyata (P<0,05). Namun, tidak terjadi perbedaan pada kelangsungan hidup ikan (P>0,05). Hasil penelitian menunjukkan bahwa penggunaan tepung ikan rucah menghasilkan pertumbuhan terbaik sebagai sumber protein utama pada pakan ikan lele sangkuriang. Kata kunci : ikan lele, pertumbuhan, at satiation, tepung ikan

Fish oil supplementation in commercial diet on growth of Anguilla bicolor bicolor Mukti, Retno Cahya; Utomo, Nur Bambang Priyo; Affandi, Ridwan
Jurnal Akuakultur Indonesia Vol. 13 No. 1 (2014): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

ABSTRACT This study was aimed to determine the effect of fish oil addition on growth performance and fatty acid composition of eel Anguilla bicolor bicolor. Freswater eel at an initial body weight of 9.90± 0.05 g were maintained in aquarium with volume of 120 L at stocking density of 1 g/L for 40 days. This research applied complete randomized design with four treatments addition of fish oil, i.e. 0%, 5%, 10%, and 15%. All treatments were carried out in triplicate. Feed given as much as 3% of the fish biomass a day for four times at 06:00, 11:00, 16:00, and 21:00. The results showed that the addition of fish oil in the diet have different effects (P<0.05) on specific growth (0.88–1.36%), feed efficiency (30.18–48.53%), protein retention (14.57–20.24%), fat retention (16.77–52.49%), energy retention (12.38–20.20%), and hepatosomatic index (1.72–2.72%) whereas the survival showed no difference (P>0.05) at 100%. In the fatty acid composition total of unsaturated fatty acid composition was 30.91–40.95%, n-3 fatty acids was 6.10–8.19%, and n-6 fatty acids were 6.18–8.19%. In conclusion, the addition of fish oil in the diet of freshwater eel Anguilla bicolor bicolor can be done up to 5% (13% fat content of diet). Keywords: Anguilla bicolor bicolor, fish oil, growth performance, fatty acid composition ABSTRAK Penelitian ini bertujuan untuk menguji pengaruh penambahan minyak ikan terhadap kinerja pertumbuhan dan komposisi asam lemak ikan sidat Anguilla bicolor bicolor. Ikan sidat dengan bobot 9,90±0,05 g dipelihara dalam akuarium dengan volume 120 L pada padat tebar 1 g/L selama 40 hari. Penelitian ini menggunakan rancangan acak lengkap terdiri atas empat perlakuan penambahan minyak ikan pada pakan sebesar 0%, 5%, 15%, dan 15%. Semua perlakuan terdiri atas tiga ulangan. Pakan diberikan sebanyak 3% dari biomassa ikan dan diberikan sebanyak empat kali sehari yaitu pukul 06.00, 11.00, 16.00 dan 21.00. Hasil penelitian menunjukkan bahwa penambahan minyak ikan dalam pakan memberikan pengaruh yang berbeda (P<0,05) terhadap spesific growth rate (0,88– 1,36%), efisiensi pakan (30,18–48,53%), retensi protein (14,57–20,24%), retensi lemak (16,77–52,49%), retensi energi (12,38–20,10%), dan indeks hepatosomatik (1,72–2,72%). Sintasan tidak menunjukkan adanya perbedaan (P>0,05) yaitu 100%. Pada komposisi asam lemak dihasilkan total komposisi asam lemak tidak jenuh 30,91– 40,95%, asam lemak n-3 6,10–8,19%, dan asam lemak n-6 6,18–8,19%. Dengan demikian, dapat disimpulkan bahwa penambahan minyak ikan dalam pakan ikan sidat Anguilla bicolor bicolor dapat dilakukan sampai dengan 5% (kadar lemak pakan 13%). Kata kunci: Anguilla bicolor bicolor, minyak ikan, kinerja pertumbuhan, komposisi asam lemak

Evaluation of incubated defatted rubber seed meal with sheep rumen liquor for Pangasius diet Suprayudi, Muhammad Agus; Irawan, Winda Styani; Utomo, Nur Bambang Priyo
Jurnal Akuakultur Indonesia Vol. 13 No. 2 (2014): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

ABSTRACT The research evaluated the use of rubber seed meal (Hevea brasiliensis; RBS) incubated with sheep rumen liquor as a subtitution of soybean meal in catfish Pangasionodon sp. diet. The fish was cultured for 40 days and fed with the experimental diet containing RBS at five different diet compositions regarding to soybean meal substitution level, i.e. 0% (control), 12%, 23%, 34%, and 44%. Feeding was done three times a day to satiation. No significant different was found on fish-protein retention and survival rate in all treatments. Based on the study result, the use of rubber-seed meal (Hevea brasiliensis; RBS) incubated with sheep rumen liquor could substitute soybean meal in catfish Pangasionodon sp. diet. Keywords: Hevea brasiliensis, Pangasionodon sp., catfish, sheep rumen liquor, rubber seed meal ABSTRAK Penelitian ini mengevaluasi penggunaan tepung bungkil biji karet (Hevea brasiliensis; TBBK) yang diinkubasi dengan cairan rumen domba sebagai pengganti tepung bungkil kedelai pada pakan ikan patin Pangasionodon sp. Pemeliharaan ikan dilakukan selama 40 hari dengan pemberian lima komposisi pakan berbeda sesuai tingkat substitusi tepung bungkil kedelai oleh tepung bungkil karet. TBBK yang ditambahkan untuk mengganti bungkil kedelai adalah sebesar 0%, 12%, 23%, 34% dan 44%. Pemberian pakan dilakukan selama tiga kali sehari secara at satiation. Tidak ditemukan perbedaan signifikan (P>0,05) pada nilai retensi protein dalam tubuh dan kelangsungan hidup ikan uji pada semua perlakuan. Berdasarkan hasil penelitian, dapat disimpulkan bahwa tepung bungkil biji karet yang diinkubasi dengan cairan rumen domba dapat digunakan sebagai pengganti bungkil kedelai pada pakan ikan patin Pangasionodon sp. Kata kunci: Hevea brasiliensis, Pangasionodon sp., patin, rumen domba, tepung biji karet

The growth and meat quality of Siamese catfish fed different level of protein Purnomo, Novieanto; Utomo, Nur Bambang Priyo; Azwar, Zafril Imran
Jurnal Akuakultur Indonesia Vol. 14 No. 2 (2015): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

ABSTRACT This study was to evaluate growth performance and meat quality of Siamese catfish Pangasianodon hypophthalmus fed on commercial diets with different protein levels. Fish with average initial body weight of 33,61 g were reared in hapa (2×2×1 m3) at density of 30 ind/hapa. Fish were hand-fed with experimental diet to satiety twice daily for 60 days. Experimental design was set in completely randomized design. Each treatment was done in triplicates. Experimental diets were a commercial diet to contain four differennt levels of protein; i.e. diet A (18%), diet B (23%), diet C (28%), and diet D (32%). Specific growth rate (SGR), feed conversion ratio (FCR), protein (RP) and lipid (RL) retention, hepatosomatic index (HSI), lipid and glycogen content of liver, protein and lipid content of meat, edible portion, and fillet textural were calculated. The results of the experiment showed that the highest weight gain and SGR, lowest FCR and meat protein were obtained in the group of fish fed 23%–32% protein diets. In conclusion, 23% protein diets gave the best growth performance and meat quality of Siamese catfish. Keywords : growth performance, meat quality, commercial diet, Pangasianodon hypophthalmus ABSTRAK Penelitian ini bertujuan untuk mengevaluasi kinerja pertumbuhan dan kualitas daging ikan patin siam Pangasianodon hypophthalmus yang diberi pakan komersial dengan kandungan protein berbeda. Ikan patin yang digunakan dengan bobot awal 33,61 g ditebar sebanyak 30 ekor/hapa ukuran 2×1×1 m3 dan ikan diberi pakan percobaan dua kali sehari secara at satiation selama 60 hari. Penelitian ini didesain dalam rancangan acak lengkap dengan empat perlakuan dan tiga ulangan. Pakan yang digunakan adalah pakan komersial dengan empat kandungan protein yang berbeda: pakan A (18%), pakan B (23%), pakan C (28%), dan pakan D (32%). Parameter uji pada penelitian ini adalah laju pertumbuhan spesifik (SGR), rasio konversi pakan (FCR), retensi protein (RP), retensi lemak (RL), indeks hepatosomatik (HSI), lemak dan glikogen hati, protein dan lemak daging, serta tekstur daging. Hasil penelitian menunjukan bahwa penambahan bobot tubuh, SGR tertinggi, FCR terendah, dan protein daging diperoleh pada perlakuan protein pakan 23%–32%. Hasil penelitian ini menunjukkan bahwa perlakuan protein pakan 23% memberikan kinerja pertumbuhan dan kualitas daging ikan terbaik. Kata kunci: kinerja pertumbuhan, kualitas daging, pakan komersial, Pangasianodon hypophthalmus

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