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All Journal Jurnal Bios Logos Natural B Biosaintifika: Journal of Biology & Biology Education Berkala Penelitian Hayati Jurnal Kreativitas dan Inovasi (Jurnal Kreanova) Sewagati Molekul: Jurnal Ilmiah Kimia
Dwi Kusuma Wahyuni
Biology Department, Faculty Of Sciences And Technology, Airlangga University
Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemistry Economics, Econometrics & Finance Education Engineering Environmental Science Immunology & microbiology Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Medicine & Pharmacology Public Health Social Sciences Other

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Perkembangan Kultur Daun Aglaonema sp. dengan Perlakuan Kombinasi Zat Pengatur Tumbuh NAA dan 2,4-D dengan BAP (The Leaf Culture Development of Aglaonema sp. Treated by Combination of NAA, 2,4-D and BAP as Growth Regulators) Wahyuni, Dwi Kusuma; Prasetyo, Dedy; Hariyanto, Sucipto
JURNAL BIOS LOGOS Vol 4, No 1 (2014): JURNAL BIOSLOGOS
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/jbl.4.1.2014.4837

Abstract

Abstrak Tujuan dari penelitian ini adalah menentukan pengaruh kombinasi NAA dan 2,4-D dengan BAP terhadap perkembangan kultur daun dan konsentrasi yang sesuai untuk induksi kalus tiga kultivar Aglaonema sp. Kultivar-kultivar yang digunakan adalah Dynamic Ruby, Snow White, dan Siam Aurora. Eksplan daun dikulturkan pada medium MS padat dengan perlakuan kombinasi NAA dan 2,4-D dengan BAP. Hasil pengamatan minggu kedelapan menunjukkan ada pengaruh perlakuan zat pengatur tumbuh terhadap perubahan bentuk eksplan daun. Eksplan daun melengkung, bergelombang, membengkak, warna eksplan memucat dan membentuk kalus, tetapi hanya Aglaonema sp. cv. Dynamic Ruby yang mampu membentuk kalus. Kombinasi zat pengatur tumbuh 0,1 ppm 2,4-D and 1 ppm BAP adalah kombinasi yang sesuai untuk induksi kalus untuk Aglaonema sp. cv. Dynamic Ruby. Kata Kunci: Aglaonema, NAA, 2,4-D, BAP, kalus   Abstract   The objective of this study were to determine the effect of combination of NAA and 2,4-D with BAP toward leaf culture development and to know the best concentration of growth regulator substance to induce callus on leaf explant of three Aglaonema cultivars. The cultivars were Dynamic Ruby, Snow White, and Siam Aurora. Leaf explants were cultured on solid MS medium with addition of various concentration combination of NAA and 2,4-D with BAP. Result of eight week observation gave significantly effect to changes shape leaf. Leaf blade was curved or rolled up, swelled, leaf color became pale strands, and formed callus, but the only cultivar which formed callus was Aglaonema sp. cv. Dynamic Ruby. The conclusion of this study was combination of growth regulator substance 0,1 ppm 2,4-D and 1 ppm BAP are the appropriate combination on callus inducing for Aglaonema sp. cv. Dynamic Ruby. Keywords: Aglaonema, NAA, 2,4-D, BAP, callus
Induksi Akar Rambut Gandarusa (Justicia gendarussa Burm. f.) dengan Perlakuan Perbedaan Lama Waktu Infeksi Agrobacterium rhizogenes strain YM072001 dan A4T (The Induction of Gandarusa (Justicia gendarussa Burm. f.) Hairy Root under Different Infection Wahyuni, Dwi Kusuma; Nisa, Qonitatun; Purnobasuki, Hery; Ermayanti, Tri Muji; Prajoga, Bambang; Utami, Edy SW
JURNAL BIOS LOGOS Vol 5, No 2 (2015): JURNAL BIOSLOGOS
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/jbl.5.2.2015.10548

Abstract

Abstrak Penelitian ini bertujuan untuk menginduksi akar rambut eksplan daun Gandarusa (Justicia gendarussa Burm.f.) dengan perlakuan perbedaan lama waktu infeksi Agrobacterium rhizogenes strain YM072001 dan A4T dan mengevaluasi lama waktu infeksi terbaik untuk induksi akar rambut. Eksplan daun diinokulasi di media MS (Murashige dan Skoog) cair yang berisi bakteri Agrobacterium rhizogenes dengan OD600 = 0,1 selama 10, 20, 30, 40, 50, dan 60 menit. Kokultivasi di MS padat selama 2 hari, lalu disubkultur ke media MS padat. Data dianalisis secara deskriptif. Akar rambut mulai tumbuh pada minggu ketiga. Induksi akar rambut berhasil pada eksplan dengan perlakuan strain YMB072001 dengan lama waktu infeksi 20, 40, dan 50 menit. Strain A4T juga berhasil menginduksi eksplan membentuk akar rambut dengan perlakuan 10 menit. Lama waktu infeksi terbaik untuk strain YMB072001 adalah 20 menit dan untuk strain A4T adalah 10 menit. Kata kunci: Agrobacterium rhizogenes, akar rambut, Justicia gendarussa Burm. f., waktu infeksi   Abstract The objective of this study were to induce hairy root of Gandarusa leaf explants by differences of Agrobacterium rhizogenes infection time treatment and to evaluate the best infection time for induction. Leaf explants were inoculated on MS (Murashige dan Skoog) liquid medium with bacterial concentrations of OD600 = 0,1 for 10, 20, 30, 40, 50, and 60 minutes and 2 days co-cultivated on MS0 solid medium then sub cultured on MS0 solid medium. Data were analyzed descriptively. Hairy roots were growing on the third week. Explants were successfully induced by strain YMB 072001 at 20, 40, and 50 minutes treatment. A4T strain was successfully induced by 10 minute of treatment. The best infection time for hairy root induction in gandarusa leaf explants for YMB 072001 strains was 20 minutes and for A4T strain was 10 minutes. Keywords: Agrobacterium rhizogenes, hairy root, infection time, Justicia gendarussa Burm. f.
Co-Authors Abdulloh Abdulloh Agus Supriyanto Almando Geraldi Anika Sindhya Dewi Anjar Tri Wibowo Arif Nur Muhammad Ansori Bambang Piluharto Bambang Prajoga Bambang Prajoga Eko Wardoyo Bambang Prajoga Eko Wardoyo, Bambang Prajoga Eko Dedy Prasetyo Edy Setiti Wida Utami Edy SW Utami, Edy SW Firli Rahmah Primula Dewi Hamidah . Hery Purnobasuki Ika Sukma Dewi Intan Ayu Pratiwi, Intan Ayu Junairiah Junairiah Manikya Pramudya Muhammad Hilman Fu'adil Amin Pradana, Rizal Adistya Putra Purkan Purkan, Purkan Putri Andriani Putri Andriani Qonitatun Nisa, Qonitatun Siti Nur Hafida Siti Nur Hafida, Siti Nur Sofijan Hadi Sri Puji Astuti Wahyuningsih, Sri Puji Astuti Sri Sumarsih Sucipto Hariyanto Suhargo, Listiyani Tri Muji Ermayanti Tri Muji Ermayanti Wulandari, Melysa