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All Journal HAYATI Journal of Biosciences Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Microbiology Indonesia The Journal of Experimental Life Sciences (JELS) Agrointek Jurnal Pangan dan Agroindustri Jurnal Keteknikan Pertanian Tropis dan Biosistem Jurnal Teknologi Pertanian agriTECH Indonesian Journal of Biotechnology UNEJ e-Proceeding Chimica et Natura Acta Agroindustrial Technology Journal Menara Perkebunan

AGUSTIN KRISNA WARDANI

Universitas Brawijaya

Author-ID : 412509

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Control & Systems Engineering Earth & Planetary Sciences Education Electrical & Electronics Engineering Energy Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Materials Science & Nanotechnology Mechanical Engineering Medicine & Pharmacology Neuroscience

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Identifikasi Gen Transgenik pada Produk Susu Bubuk Kedelai dan Susu Formula Soya dengan Metode PCR (Polymerase Chain Reaction) Agustin Krisna Wardani; Annisa Alirsyah; Ana Fauziah
agriTECH Vol 37, No 3 (2017)
Publisher : Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia

The need of soybean that reached up to 2,2 million tons per year has made Indonesia imports this commodity 1,62 million tons from countries that adopt Genetically Modified (GM) soybean. By the presence of GM soybeans in Indonesia, Genetically Modified Product (GMP) labelling has need to be done. Detection of GMP can be done by using PCR. The aim of this study was to determine the presence of trangenic genetic material in soy milk powder and soy formula products to classify as GMP. Another goal was to determine the optimum annealing temperature of the primers used. Based on this study, the optimum annealing temperature of the CaMV 35S primer and the EPSPS-CP4 primer was 60oC and 59oC. The NOS terminator primer’s optimum annealing temperature was not found. 6 soy milk powder samples and 5 soy formula samples are might be determined to be using transgenic soybeans due to the presence of EPSPS-CP4 genes and CaMV 35S promotor genes. Therefore, those 11 samples were classified as GMP.ABSTRAK Kebutuhan kedelai yang mencapai 2,2 juta ton/tahun memaksa Indonesia mengimpor sebanyak 1,62 juta ton. Sebagian besar kedelai impor berupa kedelai transgenik. Dengan munculnya kedelai transgenik di Indonesia, perlu adanya pelabelan Produk Rekayasa Genetika (PRG) untuk memenuhi hak-hak konsumen. Teknik yang dilakukan untuk mendeteksi PRG salah satunya menggunakan metode PCR. Penelitian ini bertujuan untuk mengetahui ada tidaknya gen transgenik pada produk susu bubuk kedelai dan formula soya, sehingga produk dapat digolongkan sebagai PRG atau tidak. Selain itu juga bertujuan untuk mengetahui suhu annealing optimum pada primer yang digunakan. Hasil penelitian didapatkan suhu annealing optimum primer CaMV 35S promotor adalah 60oC. Sedangkan untuk primer gen EPSPS-CP4 suhu annealing optimumnya 59oC. Untuk primer NOS terminator suhu annealing optimum tidak ditemukan. Dari amplifikasi DNA sampel, 6 sampel susu bubuk kedelai dan 5 sampel formula soya terdapat sisipan gen EPSPS-CP4 dan gen Promotor CaMV 35S. Dengan demikian 11 sampel tersebut dapat dikatakan sebagai PRG. Kata kunci: Produk rekayasa genetika; PCR; formula soya; susu kedelai bubuk; kedelai transgenik

Studi Komparasi: Produksi Bioetanol Nira Batang Kelapa Sawit oleh Flokulan dan Non- Flokulan Saccharomyces cerevisiae Kafidul Ulum; Indria Purwantiningrum; Retno Dwi Yustina; Untung Murdiyatmo; Agustin Krisna Wardani
agriTECH Vol 40, No 4 (2020)
Publisher : Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia

Two types of yeast were used for bioethanol production from oil palm trunk sap, the flocculant Saccharomyces cerevisiae NCYC1195 and nonflocculant Saccharomyces cerevisiae Kyokai 7 (NCYC-479). Flocculant Saccharomyces cerevisiae is yeast that has ability to aggregate into flocks which precipitate rapidly in culture medium. The effect of urea as a nitrogen source was also investigated in this study. Some concentrations of urea were added i.e. 0%, 0.1%, 0.2%, and 0.3% (w/v) during fermentation. The purpose of this study is to obtain the best condition by strain of Saccharomyces cerevisiae and urea concentration for the highest ethanol production. The highest ethanol production and yield was obtained at 4.86% (v/v) and 0.52 (g/g) respectively, by nonflocculant Saccharomyces cerevisiae Kyokai 7 (NCYC-479) without the addition of urea.

SCREENING AND PARTIAL CHARACTERIZATION OF BACTERIOCIN FROM LACTID ACID BACTERIA ISOLATED FROM FAN PALM SUGAR (Borassus flabellifer L) Prestasia Budi Lestari; Agustin Krisna Wardani
UNEJ e-Proceeding International Conference on Agribusiness Marketing (ICAM) 2012, Faculty of Agriculture, University o
Publisher : UPT Penerbitan Universitas Jember

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Bacteriocin is a protein compound that has bactericidal action against microorganism. Bacteriocins from lactid acid bacteria are very potensial as natural food biopreservatives.The aim of this present study is to obtain the isolate of lactic acid bacteria which has apotential to produce bacteriocin from fan palm sugar, to attain the bacteriocincharacterization such as its stability against heat and proteolytic enzyme. Other goal is toobserved the inhibitory activity of bacteriocin against Gram positive and Gram negativebacteria. This research found that 2 isolates LB.9 and LB.30 have a potency to producebacteriocin. LB.9 sensitive to protease enzyme and heat labile whereas isolate LB.30sensitive to protease enzyme and heat stable. The bacteriocin are able to inhibit the growthof Gram positive and Gram negative bacteria.

Optimization of Ethanol Production from Palmyra Sap by Zymomonas mobilis using Response Surface Methodology (RSM) RUTH CHRISNASARI; AGUSTIN KRISNA WARDANI; UNTUNG MURDIYATMO
Microbiology Indonesia Vol. 5 No. 2 (2011): June 2011
Publisher : Indonesian Society for microbiology

Ethanol is believed to be one of the best alternatives to replace gasoline, because ethanol is a renewable energy source and environmentally friendly. The present study focuses on the optimization of palmyra sap as a source for ethanol production. Statistical experimental design using Box-Wilson central composite design (CCD) was used to optimize the quantitative effects of sugar, urea, and inoculum concentration on ethanol production. It was found that palmyra sap could be used as a substrate for ethanol production using Zymomonas mobilis (NRRL B-14234). A maximum ethanol concentration of 58.97 g L-1 was obtained after optimizing the parameters of fermentation. The optimum values of sugar, urea, and inoculums concentration were 206.01 g L-1, 3.16 g L-1, and 23.05% (v v-1), respectively, with ethanol yield of 0.3039 g g-1. A high similarity was observed between the predicted and experimental results, which reflected the accuracy and applicability of RSM to optimize the process for ethanol production.

Cloning of α-L-arabinofuranosidase Genes and Its Expression in Escherichia coli: A Comparative Study of Recombinant Arabinofuranosidase Originatingin Bacillus subtilis DB104 and Newly Isolated Bacillus licheniformis CW1 MOCHAMAD NURCHOLIS; NIKNIK NURHAYATI; IS HELIANTI; MARIA ULFAH; BUDIASIH WAHYUNTARI; AGUSTIN KRISNA WARDANI
Microbiology Indonesia Vol. 6 No. 1 (2012): March 2012
Publisher : Indonesian Society for microbiology

Arabinofuranosidase (Abfa) is one of the most important enzymes involved in degradation of lignocelullose biomass. Two genes encoding α-L-Arabinofuranosidase (abfA), each from Bacillus subtilis DB104 (abfAa1) and an indigenous Indonesian B. licheniformis CW1 (abfAb3), were cloned by the PCR approach and expressed in Escherichia coli. Sequences analysis of abfAa1 and abfAb3 revealed that each consists of 1721 and 1739 base pairs long DNA, respectively. Each clone contains a hypothetical open reading frame of 1503 and 1509 bp that encode an Abfa protein of 500 and 502 amino acids for abfAa1 and abfAb3, respectively. The deduced amino acid sequence of AbfaB3 shares 75% identity to that of AbfaA1. The recombinant enzymes were expressed constitutively in E. coli. Partial characterization of those enzymes revealed that the AbfaA1 and AbfaB3 were optimally active at 50 ºC and 60 ºC at pH 6, respectively. Thermostability studies of the recombinant enzymes with p-nitrophenyl α-L-arabinofuranoside at their optimal conditions showed that up to 50% AbfaA1 activity was lost after 5 h incubation at 50 ºC, whereas the AbfaB3 retained its activity over 75% after 12 h pre-incubation oat 60 ºC. This thermostability study of recombinant AbfaB3 showed for the first time that the arabinofuranosidase from B. licheniformis is a thermostable enzyme. The recombinant enzyme showed a higher optimal reaction temperature (60 ºC) in comparison to the previously reported thermostable arabinofuranosidase. The thermostable AbfaB3 has a potential to be applied to the degradation of lignocellulose biomass synergistically with thermostable xylanases, for instance in the production of xylo-oligosaccharides.

Studi Kelayakan Finansial dan Kebutuhan Utilitas Proses Produksi “Stiff Oorid Mango” Ugali Instant Kaya Nutrisi dalam Upaya Penanggulangan Malnutrisi pada Anak – Anak di Kenya - Afrika Halimatus Sa'diyah; Aji Sutrisno; Agustin Krisna Wardani; Bambang Susilo
Jurnal Keteknikan Pertanian Tropis dan Biosistem Vol 2, No 1 (2014)
Publisher : Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (344.858 KB)

Malnutrisi merupakan masalah yang banyak terjadi di negara berkembang termasuk Kenya. Kasus malnutrisi yang banyak terjadi di Kenya adalah protein-energi malnutrisi dan defisiensi vitamin A. Pengembangan ugali (Stiff Oorid Mango) melalui fortifikasi mangga dan kacang tunggak dapat mengatasi masalah protein-energi malnutrisi dan defisiensi vitamin A di Kenya. Selain itu, pemilihan mangga sebagai Fortifikasi β-karoten yang pro-vitamin A pada produksi Stiff Oorid Mango bertujuan untuk memaksimalkan penggunaan mangga yang selama ini terbuang di Kenya. Kelimpahan bahan baku tersebut dapat menjadi peluang besar bahwa produk baru Stiff oorid mango dapat diaplikasikan di Industri. Untuk mengetahui kelayakan produksi Stiff oorid mango, maka dilakukan analisis kelayak finansial dan kebutuhan utilitas. Aspek kelayakan finansial yang dianalisis yaitu Harga Pokok Produksi (HPP), Break Even Point (BEP), R/C Ratio dan Net Present Value (NPV). Sedangkan, aspek kebutuhan utilias yang dianalisis kebutuhan water system dan Sewage System. Penelitian ini bertujuaan mengetahui analisis finansial dan kebutuhan utilitas proses produksi Stiff oorid mango bila diterapkan di Industri. Berdasarkan hasil perhitungan kelayakan finansial, harga pokok produksi setiap kemasan Stiff Oorid Mango adalah Rp 2.020, atau setara K Sh. 17,91, dengan nilai Break Even Point (BEP) yaitu 12.739 kemasan, dan R/C ratio 1.41 sehingga produk tersebut efisien untuk dijalankan karena >1. Analisis utilitas stiff oorid mango ini menganalisisis kebutuhan water system (distribusi pengolahan air bersih) bersumber dari dept well yang diolah dengan aerasi dan sand filter, dan Sewage System (pengolahan limbah) menggunakan metode fisik meliputi penyaringan, equalisasi, penyeragaman, pendinginan dan filter pasir dan metode biologis meliputi kolam aerasi dan lagoon. Kata Kunci : Stiff oorid mango, Malnutrisi, Analisis Kelayakan Finansial, dan Kebutuhan Utilitas

ISOLASI DAN KARAKTERISASI PARSIAL ENZIM SELULASE BAKTERI SELULOLITIK HASIL ISOLASI DARI LIMBAH PADAT TAPIOKA (ONGGOK) Rhytia Ayu C. Putri; Agustin Krisna Wardani
Agroindustrial Technology Journal Vol 2, No 2 (2018): Agroindustrial Technology Journal
Publisher : Universitas Darussalam Gontor

Proses hidrolisis bahan berselulosa tinggi memerlukan perlakuan pendahuluan untuk memisahkan lignin dan hemiselulosa dari selulosa, tetapi proses ini kurang efisien dan tidak ramah lingkungan. Enzim selulase adalah alternatif untuk menghidrolisa selulosa karena sifat kerja enzim yang spesifik dan tidak menghasilkan limbah yang berbahaya serta diharapkan mampu membantu memudahkan proses perlakuan pendahuluan. Bakteri selulolitik penghasil enzim selulase dapat diisolasi dari limbah pertanian yang banyak mengandung selulosa seperti limbah padat tapioka (onggok). Hasil penelitian menunjukkan bahwa 2 isolat yaitu TO4 dan TO5 merupakan isolat yang potensial menghasilkan selulase. Enzim selulase dari isolat TO4 dan TO5 memiliki aktivitas optimum pada pH 5 dan suhu 50°C dengan aktivitas enzim selulase sebesar 0,597 U/mg untuk TO4 dan 0,557 U/mg untuk TO5. Hasil uji kemampuan enzim dalam hidrolisis bahan berselulosa tinggi seperti bagas tebu menunjukkan bahwa enzim selulase dari isolat TO4 mampu menghidrolisis selulosa pada bagas tebu sebanyak 15,8% setelah direaksikan selama 120 jam.Kata kunci:Bakteri Selulolitik, Bagas Tebu, Onggok, Selulase, Selulosa

Genomic and Haplotype Comparison of Butanol Producing Bacteria Based on 16S rDNA Ekwan Nofa Wiratno; Suharjono Suharjono; Agustin Krisna Wardani
Journal of Tropical Life Science Vol. 6 No. 1 (2016)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.06.01.01

High butanol demand for transportation was support to butanol development. Exploration of butanol producing bacteria using genome comparison and biogeography give role to butanol industrialization. Objective of this research are butanol production, genome comparison and haplotype analysis of butanol producing bacteria from Ranu Pani Lake sediment using 16S rDNA sequences. Highest butanol concentrations were resulted by Paenibacillus polymyxaRP 2.2 isolate (10.34 Â± 0.00 g.l-1) then Bacillusmethylotrophicus RP 3.2 and Bacillusmethylotrophicus RP 7.2 isolate (10.11 Â± 0.01 g.l-1 and 9.63 Â± 0.01 g.l-1). Number of bases (T, C, A, G) of group 1 are similar, but different with group 2. Least G+C content is Clostridium saccharobutylicum Ox29 (51.35%) and highest is Bacillus methylotrophicus RP 7.2. Conserve region (1044 bp) of 16S rDNA higher then variative region (367 bp). The number of 319 bp is PIS whereas single tone as much as 48 bp. There are 17 conserves sequences. All of butanol producing bacterial sequences was clustered to 8 haplotype. Based on source of bacteria, there are three group of haplotype. Group A was isolated from Asia, group B was isolated from America and group C was isolated from Europe.

Leclercia adecarboxylata C12, The Newly Isolated Cellulose-degrading Bacteria from Indonesian Coffee Pulp Agustin Krisna Wardani; Ajeng Astrini Brahmanti; Erryana Martati
HAYATI Journal of Biosciences Vol. 30 No. 3 (2023): May 2023
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.30.3.588-595

Culturable cellulose-degrading microorganisms were collected from Arabica coffee pulp in East Java, Indonesia. Fifty isolates were obtained, and thirty-three isolates showed hydrolyzing zone on Carboxy Methyl Cellulose agar plates after Congo-Red staining. The highest specific CMCase activity was observed by isolates C12, identified as Leclercia adecarboxylata based on 16S-rRNA gene sequence analysis. SDS-PAGE of Leclercia adecarboxylata C12 cellulase revealed two bands with a molecular mass of 95.49 and 81.28 kDa, respectively. Activity gel analysis showed the cellulolytic ability of Leclercia adecarboxylata C12 cellulase by clear zone formation. The optimal CMCase activity was achieved at 50°C and pH 9, and the activity retained 47% of its initial activity after incubation at 50°C for 90 minutes. The purified enzyme remains stable from pH 5 to 10, with 77% of its maximum activity. The activity of CMCase was stimulated by the presence of K+, Ca2+, Mg2+, and Fe3+, while SDS and EDTA reduced its activity. The current study shows that the thermostable-alkalophilic cellulase produced by Leclercia adecarboxylata C12 is very promising for industrial applications.

Effect of Reducing Sugar and Total Nitrogen to Ethanol Production from Molasses by Saccharomyces cerevisiae Ekwan Nofa Wiratno; Tri Ardyati; Agustin Krisna Wardani
The Journal of Experimental Life Science Vol. 4 No. 2 (2014)
Publisher : Postgraduate School, Universitas Brawijaya

Indonesia's oil production has declined, while demand for derivative products is increasing. Objective of this research are to understand effect of reducing sugar and total nitrogen variation to ethanol production and fermentation efficiency, cell viability, acidity, temperature, dissolved oxygen with molasses by Saccharomyces cerevisiae (SAF Instant). Step of this research consist of determination of reducing sugar, ethanol fermentation, total nitrogen determination, ethanol determination and data analysis. Treatment of reducing sugar (GR) and total nitrogen (N) (g.L-1) that are GR 100 N 0, GR 100 N 6, GR 100 N 10, GR 125 N 0, GR 125 N 6 and GR 125 N 10. Fermentation was carried out for 72 hours with three replications. Observation parameters every 24 hours are ethanol and reducing sugar concentration, temperature, acidity and dissolved oxygen. Highest ethanol resulted from GR 125 N 6 (3.68 g.L-1) and GR 100 N 6 (3.53 g.L-1). Low reducing sugar consumption inhibited by by-product of yeast metabolism and molasses chemical compound, lead leaves high sugar concentration (> 80 g.L-1). GR 100 N 6 and GR 125 N 6 have highest fermentation efficiency (69 and 57 %). There was no increase in temperature and decrease in pH significantly (α>0.05). Dissolved oxygen decreased significantly (α>0.05) at the early of fermentation and decrease until the end of fermentation. Total nitrogen 6 g g.L-1 has the highest fermentation efficiency. Keywords: ethanol, molasses, reducing sugar, Saccharomyces cerevisiae, total nitrogen

Co-Authors - Fadlurrahman, - . Harijono Adi Syamsuri Agustin Krisna Wardhani Ajeng Astrini Brahmanti Aji Sutrisno AJI SUTRISNO Aji Sutrisno Ajrullah, Mauludy Jutta Ana Fauziah Anita Fitri Astuti Anita Fitri Astuti Anita Kusuma Finalissari Annisa Alirsyah Annisa Fadlilah Koos Cahyani Annisa Fadlilah Koos Cahyani Asma' Khoirun Nisa Atmiral Ernes Azmi Nahdhiyati Fathimah Azmy Nahdhiyati Fathimah, Azmy Nahdhiyati BAMBANG SUSILO Budiasih Wahyuntari Christina Ekawati Halim Dadang Suhendar Deden Eris Devi arianty Dian Widya Ningtyas Dwi Arinda Syahputri Dwi Arinda Syahputri Dwi Okta Indriani Dwi Okta Indriani Efendi Oulan Gustav Hakim Nata Buana Eko Sutrisno Hawusiwa Eko Sutrisno Hawusiwa ekwan nofa wiratno Ekwan Nofa Wiratno Ekwan Nofa Wiratno Elok Puji Kurnia Sari Endrika Widyastuti Erni Sofia Murtini Erryana Martati Fabryana Noor Anggita Putri Fabryana Noor Anggita Putri Fadeli Muhammad Habibie Faula Libna Nabela Fenty Nurtyastuti Eka Pertiwi Feronika Heppy Sriherfyna Fithri Choirun Nisa Grace Wijaya Gunawan, Ellen Fenix Halimatus Sa'diyah Hardanti, Sri Harsojo Harsojo Harsojo Harsojo Harsojo Harsojo Helmy Aditya Prabowo, Helmy Aditya Husna, Afifa Ika Rachmawati Wardani Ika Rachmawati Wardani Ika Yuli Andarti Ika Yuli Andarti Indah Kusumawardini Indria Purwantiningrum IS HELIANTI Is Helianti IS HELIANTI Jamhari Jamhari Joni Kusnadi Kafidul Ulum Kezia Abib Yerah Tjandra Kharisma Nafia Safitri Laili One Januarista Lauren Chrisya Wiguna Lia Oriana Nindita Lia Ratnawati Luqvia Noer Islami Syamsudin Maria Ulfah Marisa Zakiya Ulfa Marisa Zakiya Ulfa, Marisa Zakiya Mochamad Nurcholis Muhamad Taufiqul Naufal Muhamad Tommy Adrian Muhamad Tommy Adrian Muhammad Nur Sigit Harianto Muhammad Nur Sigit Harianto Niknik Nurhayati NIKNIK NURHAYATI Nur Ida Panca Nugrahini Pramesti, Nadya Shafa Pratidina Andayani Prawiro, Sumarno Reto Prestasia Budi Lestari Reno Nasrudin Salas Retno Dwi Yustina Rhytia Ayu C. Putri Riris Wahyuhapsari Riris Wahyuhapsari Risqia Adinda Putri RR. Ella Evrita Hestiandari RUTH CHRISNASARI RUTH CHRISNASARI Silvy Novita Antrisna Putri Silvy Novita Antrisna Putri, Silvy Novita Antrisna Sindy Hamadi Suharjono Suharjono Suminar Diyah Nugraheni SY, Muhammad Zakwan Saputra TATI NURHAYATI Tri Ardyati Tri Yudani MR UNTUNG MURDIYATMO Untung Murdiyatmo Venisa Yosephi Vicha Vitalaya Masduki Vindy Irmanita Vindy Irmanita, Vindy Widya Dwi Rukmi Putri Windra Prayoga Yuliandri, Rahmat

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