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All Journal Jurnal Ilmiah Farmasi JURNAL KIMIA SAINS DAN APLIKASI Pharmaciana: Jurnal Kefarmasian Indonesian Journal of Cancer Chemoprevention Indonesian Journal of Chemical Science Jurnal Farmasi Sains dan Komunitas Universa Medicina Acta Pharmaciae Indonesia : Acta Pharm Indo JATI EMAS (Jurnal Aplikasi Teknik dan Pengabdian Masyarakat) Current Research on Biosciences and Biotechnology International Journal of Basic and Applied Science Berkala Ilmiah Kimia Farmasi Makara Journal of Science Jurnal Bakti Untuk Negeri Molekul: Jurnal Ilmiah Kimia Welfare: Jurnal Pengabdian Masyarakat
HENDRI WASITO
Jenderal Soedirman University
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Dentistry Education Electrical & Electronics Engineering Engineering Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Nursing Physics Public Health Social Sciences Other

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Physical Stability and Sun Protection Factors Measurement of Sunscreen Preparations in Stress Storage Conditions Using Spectrophotometry Wulandari, Widi; Wasito, Hendri; Susilowati, Sri Sutji
Acta Pharmaciae Indonesia Vol 6 No 1 (2018): Acta Pharmaciae Indonesia : Acta Pharm Indo
Publisher : Pharmacy Department, Faculty of Health Sciences, Jenderal Soedirman University, Purwokerto, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.api.2018.6.1.1442

Abstract

Latar belakang : Sediaan tabir surya dapat digunakan untuk menyerap sinar matahari secara efektif terutama pada daerah emisi gelombang UV. Kondisi stress saat penyimpanan suatu sediaan dapat mempengaruhi sifat fisik dan kimiawi. Penelitian ini bertujuan untuk menentukan pengaruh kondisi stress penyimpanan terhadap stabilitas fisik dan nilai Sun Protection Factor (SPF) sebagai efektivitas dari sediaan tabir surya yang disimpan dalam tiga suhu berbeda. Metode : Penelitian eksperimental ini dilakukan dengan pemilihan sampel tabir surya dan pengujian stabilitas fisik meliputi pengamatan warna, pengamatan fisik, uji pH, uji daya sebar, uji daya lekat, serta pengukuran nilai SPF sediaan tabir surya lotion dan gel dengan spektrofotometri yang disimpan pada suhu ruang (25◦C), suhu rendah (4◦C), dan suhu tinggi (40◦C). Hasil : kondisi stress penyimpanan mempengaruhi sifat fisik sediaan berupa daya lekat, daya sebar, dan mempengaruhi secara signifikan nilai SPF sediaan (p<0,05) mulai hari ke-14 penyimpanan.
Formulasi Tablet Hisap Kombinasi Curcuma xanthoriza Roxb., Curcuma longa L., dan Zingiber officinale ‘Sunti’ Sebagai Sediaan Kemopreventif Kanker Wardhani, Setia D; Nugroho, Firster; Yulianto, Dwi; Azizah, Soffatul; Wahyono, Yogi; Wasito, Hendri
Acta Pharmaciae Indonesia Vol 4 No 1 (2016): Acta Pharmaciae Indonesia : Acta Pharm Indo
Publisher : Pharmacy Department, Faculty of Health Sciences, Jenderal Soedirman University, Purwokerto, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Kanker adalah penyebab kematian terbesar kelima di dunia dan kasus baru muncul secara signifikan. Akhir-akhir ini, produk alami yang digunakan secara luas sebagai kemopreventive untuk kanker. Penelitian sebelumnya melaporkan Curcuma zanthorrhiza Roxb., Curcuma longa L. Dan Zingiber officinale 'Sunti' memiliki aktivitas antikanker yang diuji secara in vitro dan in vivo. Saat ini, penggunaan C. Zanthorrhiza Roxb., C. Longa L.and Z. Officinale 'Sunti'is masih terbatas oleh masyarakat. Sehingga dibutuhkan suatu sediaan yang lebih praktis dalam bentuk formulasi. Penelitian ini bertujuan untuk mempersiapkan tablet hisap dengan variasi bahan pengikat polivinilpirolidon (PVP), sehingga dihasilkan formula yang memenuhi syarat uji fisik tablet hisap serta dapat diterima oleh masyarakat. Ekstrak campuran disiapkan secara granulasi basah, sifat fisik dari uji yang dilakukan meliputi penampilan fisik, keseragaman bobot dan ukuran, uji kekerasan, uji kerapuhan, dan waktu larut dan dievaluasi berdasarkan persyaratan dalam Farmakope Indonesia edisi III dan edisi IV, data yang diperoleh kemudian dianalisis dengan uji statistik ANOVA. Data responsif uji rasa hasil oleh para responden dianalisis menggunakan Kruskal Wallis. Hasil uji statistik menunjukkan bahwa formulasi tablet hisap ekstrak dengan variasi dalam tingkat PVP 2-6% menunjukkan sifat fisik yang memenuhi syarat untuk tablet hisap.
Free Chlorine Determination in Disinfectant Product using Visible Spectrophotometry based on Prussian Blue Degradation Wasito, Hendri; Siagian, Defi Srium; Fareza, Muhamad Salman
Acta Pharmaciae Indonesia Vol 9 No 2 (2021): Acta Pharmaciae Indonesia : Acta Pharm Indo
Publisher : Pharmacy Department, Faculty of Health Sciences, Jenderal Soedirman University, Purwokerto, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.api.2021.9.2.5371

Abstract

Disinfectant products with excessive chlorine could be dangerous for health and need quality control. It is important to develop an analytical method for monitoring product quality. The main objective of this work is to develop an alternative method and evaluate the analytical performance of visible spectrophotometry for determining free chlorine in disinfectant products based on the Prussian blue decomposition process. The capability of chlorine to oxidize ferrous to ferric ions makes the Prussian blue generated by ferrosulfate and potassium ferricyanide is decomposed and measured by spectrophotometer. The formation of Prussian blue was improved by optimizing some reaction conditions and assessing incubation time. Linearity, analytical concentration range, precision, accuracy, detection limit, and quantitation limit parameters were among the examined analytical parameters. The results showed that the optimum concentration of ferrosulfate, potassium ferysianide, and hydrochloric acid for Prussian blue formation was 2.0 mmol L-1, 3.0 mmol L-1, and 0.5 mol L-1, respectively, with 15 minutes incubation time after chlorine addition. Analytical performance parameters seemed appropriate for routine analysis purposes. The developed method can also be applied as an alternative analytical method to determine the free chlorine concentration of disinfection products in the market.
Phytochemical screening and purification of n-hexane fraction of Calophyllum soulattri leaves Sunarto, Sunarto; Yuliasari, Agnes; Susilowati, Sri Sutji; Wasito, Hendri; Wijaya, Triyadi Hendra; Fareza, Muhamad Salman
Acta Pharmaciae Indonesia Vol 10 No 2 (2022): Acta Pharmaciae Indonesia : Acta Pharm Indo
Publisher : Pharmacy Department, Faculty of Health Sciences, Jenderal Soedirman University, Purwokerto, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.api.2022.10.2.5858

Abstract

Background: Calophyllum soulattri Burm F. is widely utilized in traditional medicine. It is necessary to identify secondary metabolites from C. soulattri leaves to determine the pharmacologically active chemicals. Objective: This study aimed to screen the phytochemical content and purify the n-hexane fraction of C. soulattri leaves from Banyumas, Indonesia. Methods: The n-hexane fraction was macerated with methanol, followed by liquid-liquid fractionation with n-hexane. The n-hexane fraction was tested for flavonoids, triterpenoids/steroids, saponins, and phenols using the test tube method. In addition, the compounds were purified using column chromatography. The purified compound was identified by the Liebermann-Burchard reagent, which was compared with commercially available steroid drugs as reference. Results: Phytochemical analysis revealed that the n-hexane fraction of C. soulattri leaves contained secondary metabolites such as flavonoid, steroid, and phenol compounds. Analyses with the Liebermann-Burchard reagent indicated that the purified compound was potentially a steroid. Conclusion: The compound extracted from the n-hexane fraction of C. soulattri leaves was expected as a steroid.
Potential antidepressant activity of n-hexane extract from old Areca catechu Nut by reducing depressive-like state in Swiss albino male mice Novianty, Riryn; Yuharmen; Sofiyanti, Nery; Indriani, Dwi Wahyu; Wasito, Hendri; Ya'la, Fitra Audhi
Current Research on Biosciences and Biotechnology Vol. 6 No. 1 (2024)
Publisher : Institut Teknologi Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/crbb.2024.6.1/6NKD6D9X

Abstract

Depression is a psychological disorder caused by an imbalance of neurotransmitters that can be managed with antidepressants. One of the local plants with the potential as an antidepressant is areca nut. However, its’ antidepressant effects in nonpolar solvents have not been studied recently. Equipping the extract to animals sub-chronically could mimic the clinical antidepressant treatment. Thus, the current investigation studied the antidepressant action of the hexane extract of old areca nut by utilizing acute and sub-chronic FST. This research started with extracted areca nut using n-hexane and continued with phytochemical tests. During FST, the animals were treated with n-hexane extract (50 and 100 mg/kg), fluoxetine (20 mg/kg), and saline (0.1 mL/20 g). The phytochemical test of the extract showed positive results from the content of secondary metabolites, namely saponins and steroids. n-Hexane extract at a dose of 50 mg/kg gave the best action in decreasing the immobility period. After sub-chronic medication, the secondary metabolites of the extract did not induce any toxic effects. This study’s findings imply that depression may be treated with conventional medicine, such as old areca nut extract.
OPTIMALISASI SANTRI SADAR KESEHATAN DAN OBAT DI PONDOK PESANTREN MAHASISWA wasito, Hendri; sa'dudin, Ihsan; Fareza, M. Salman; Rehana
Jurnal Bakti untuk Negeri Vol 4 No 1 (2024): JBN
Publisher : Sekolah Tinggi Ilmu Kesehatan ISFI Banjarmasin

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36387/jbn.v4i1.1809

Abstract

Health-related problems in Islamic boarding schools or Pesantren rarely get the attention of its residents and the surrounding community. An-Najah Student Islamic Boarding School is one of the Islamic boarding schools in the Banyumas area. In the health sector, the student or santri have not been optimal in paying attention to the health of the surrounding environment both in dormitories and dorm rooms. Therefore, health efforts in the form of promotive, preventive and curative were needed. The purpose of community service activities was to increase the knowledge and awareness of santri as well as the management of Pesantren related to health and proper management of medicines. In addition, activities were also carried out to improve facilities and infrastructure to support health in realizing clean and healthy Islamic Boarding Schools. The activity was carried out by adopting the Guidelines for the Implementation and Development of Islamic Boarding Health Units that had been issued by the Indonesian Ministry of Health in 2013. The results of community service activities were in the form of increasing awareness and knowledge of the students on proper health and drug management and improving health facilities and infrastructure in Islamic Boarding Schools.
Authentication of Shallots from Brebes using Gas Chromatography Fingerprinting Technique Combined with Chemometrics Pradina, Yana Setyani; Puteri, Adelia; Rachma, Gina Fauzia; Balqis, Nazwa; Anggraini, Gerli Puspita; Wasito, Hendri
Makara Journal of Science Vol. 28, No. 3
Publisher : UI Scholars Hub

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Abstract

Shallots from Brebes, also called Bima Brebes, have a more pungent aroma compared to other varieties. Its high demand results in increased prices in the market, leading to frequent cases of fraud wherein Bima Brebes shallots are replaced with other types of shallots. This study aimed to develop an analytical method using gas chromatography–flame ionization detector (GC-FID) fingerprinting combined with chemometrics to authenticate Bima Brebes shallots. Essential oils were extracted through ultrasonic hydrodistillation, followed by organoleptic, refractive index, GC-FID fingerprinting and chemometric analysis. The yield value of the five studied shallot varieties ranged from 0.02% to 0.08% w/w. Meanwhile, the organoleptic tests and refractive index values showed minimal differences among the five varieties. The GC-FID analysis revealed approximately 149 chromatogram peaks, and chemometric analysis, including principal component analysis, partial least squares-discriminant analysis, and hierarchical cluster analysis, was used to group and differentiate the chromatogram profiles of the five shallot varieties based on their types. Therefore, this method can be used as an alternative analysis technique for authenticating Bima Brebes shallots.
Forced Degradation and Stability Testing of Chloramphenicol Ear Drops Using Derivative Spectrophotometry Combining with Chemometrics Rahmah, Annisa; Wijaya, Triyadi Hendra; Wasito, Hendri
Jurnal Ilmiah Farmasi Vol. 21 No. 1 (2025): Jurnal Ilmiah Farmasi
Publisher : Universitas Islam Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20885/jif.vol21.iss1.art5

Abstract

Background: Chloramphenicol eye drops have a lower stability than solid dosage forms. Hence, it is necessary to assess their stability. One stability test that can be conducted is the forced degradation approach, which involves applying stress conditions that are more severe than those used in accelerated stability testing.Objective: This study used forced degradation to explore the stability profile of chloramphenicol ear drops.Methods: Stability analysis was carried out using a derivative spectrophotometric instrument combined with chemometric analysis. The forced degradation study was conducted by exposing the sample to three conditions: acidic (0.1 N HCl at 80°C for 2 hours), alkaline (0.1 N NaOH at 80°C for 2 hours), and heat (90°C for 4 hours). Principal Component Analysis (PCA) and Partial Least Squares–Discriminant Analysis (PLS-DA) were utilized for the chemometric analysis. Results: Sequential chloramphenicol observations with a zero to third derivative show a maximum wavelength of 278, 260, 234, and 292 nm. According to stability studies with forced degradation, chloramphenicol tended to degrade under alkaline and thermal conditions compared to acidic conditions. A typical grouping pattern amongst forced degradation treatments is revealed by chemometric analysis, which characterizes chloramphenicol's stability profile under different experimental settings.Conclusion: The UV-Vis spectrophotometric approach, both non-derivative and derivative, can describe changes in chloramphenicol degradation profiles, although the specific degradation products generated remain unknown.
Optimalisasi Peran Sekolah dan Keluarga dalam Menumbuhkan Kesadaran Pengelolaan Sampah pada Anak Usia Dini Wasito, Hendri; Nuryanti, Nuryanti; Baroroh, Hanif Nasiatul; Utami, Vitis Vini Fera Ratna; Sholihat, Nia Kurnia; Hasan, Nahrul; Wahyudi, Ari; Sari, Suci Wulan
Welfare : Jurnal Pengabdian Masyarakat Vol. 3 No. 2 (2025): Welfare : June 2025
Publisher : Fakultas Ekonomi dan Bisnis Islam, IAIN Kediri

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30762/welfare.v3i2.2215

Abstract

Waste management remains a critical issue in schools due to low awareness of clean living and limited knowledge. This community service aimed to foster early waste awareness at Azalia Integrated Islamic Kindergarten using a Participatory Action Research (PAR) approach. Activities included initial observation, game-based education, teacher-parent training, improved educational tools, and mentoring. Evaluations through pre- and post-tests showed significant improvement in both student and parent understanding of waste sorting. The program cultivated a collective awareness of waste management and is expected to continue as part of a clean and healthy life.
Nephroprotective effect and untargeted metabolomic analysis of celery extract against diethylene glycol induced nephrotoxicity in rats Wijaya, Triyadi Hendra; Ekowati, Heny; Wasito, Hendri; Maryellen, Geeta; Silitonga, Tasya Melinda; Oktavian, Firdaus
Universa Medicina Vol. 44 No. 2 (2025)
Publisher : Faculty of Medicine, Universitas Trisakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18051/UnivMed.2025.v44.190-200

Abstract

Background Diethylene glycol (DEG) is known for its nephrotoxic effects as a contaminant. Recent investigations suggest that celery extract potentially protects against DEG-induced renal toxicity, warranting an exploration of the non-targeted metabolomic profile of celery in renal tissue. This study aimed to determine celery nephroprotective effects and identify potential biomarkers using an untargeted metabolomic  celery profile in DEG-induced kidney injury in male rats. Methods An experimental study was conducted involving 24 male Wistar rats, randomized into control and three treatment groups receiving DEG at doses of 1, 3, and 5 g/BW for three days. After 14 days, all rats were sacrificed. For the celery nephroprotective and metabolomic study, 27 male rats were randomized into three groups:  control, DEG group at 3g/BW, and DEG-celery group (receiving celery extract 200mg/kg BW single dose for 6 days). After 14 days, blood was tested for creatinine level and untargeted metabolomic analysis using liquid chromatography-high resolution mass spectrometry. One-way ANOVA was used to analyze creatinine data. Results Diethylene glycol at doses of 1 and 3 g/kg BW caused mild renal damage, while 5 g/kg BW resulted in moderate damage. Creatinine levels in the normal, DEG-induced, and DEG-celery groups were 0.55 ± 0.14, 0.71±0.1, and 0.86± 0.17 mg/dL, respectively, but not statistically significantly different between groups (p>0.05). DEG lead tryptophan and phenylalanine levels increased 45-fold and 123-fold, respectively. Celery reduced levels of these metabolites 21-fold and 65-fold, respectively. Conclusion Diethylene glycol led to mild to moderate renal damage. Celery effectively diminished metabolite levels associated with renal damage, specifically tryptophan and phenylalanine.
Co-Authors Adi Wibowo Aisy Prita Syafina AMIN FATONI Anggraini, Gerli Puspita ARI WAHYUDI Ary Madina Asrada, Syahdan Azizah, Soffatul Balqis, Nazwa Choironi, Nur Amalia Dwi Yulianto Fareza, M. Salman Fareza, Muhamad Salman Fischellya, Dafi Fitri Fauziah Hayati Ganjar Wahyu Rahardian Gilas Pandu Nur Fakhri Hafizah, Ilmi Nur Hafizah, Ilmi Nur Hanif Nasiatul Baroroh Hasan, Nahrul Hening Pratiwi, Hening Heny Ekowati Indriani, Dwi Wahyu Karyati, Eva Karyati, Eva Khairun, Medi Khairun, Medi Maryellen, Geeta Monica, Sausa Mustikaningtyas, Ika Nery Sofiyanti Nia Kurnia Sholihat, Nia Kurnia Novianty, Riryn Nugroho, Firster Nuryanti Nuryanti Oktavian, Firdaus Pradina, Yana Setyani Puteri, Adelia Rachma, Gina Fauzia Rahardian, Ganjar Wahyu Rahmah, Annisa Rehana Rehana, Rehana Ridha, Kirana Shafa Sa'dudin, Ihsan Samudra, Genta Hafied Naga Sari, Suci Wulan Sarmoko Sarmoko Sausa Monica Setiyabudi, Lulu Siagian, Defi Srium Silitonga, Tasya Melinda Solihat, Nia Kurnia Sri Sutji Susilowati Sri Sutji Susilowati Sunarto Sunarto Triyadi Hendra Wijaya Tsabitatun Ni'mah Tuti Sri Suhesti Utami, Hamidah Raisa Utami, Hamidah Raisa Utami, Vitis Vini Fera Ratna Vikarosa, Charlina Detty Vikarosa, Charlina Detty Wahyono, Yogi Wardhani, Setia D WULANDARI, WIDI Wulandari, Widi Ya'la, Fitra Audhi Yuharmen Yulia Yulia Yuliasari, Agnes