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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Jurnal Kedokteran Brawijaya Majalah Kedokteran Bandung Universa Medicina Jurnal Kedokteran Hewan
M. Aris Widodo
Bagian Farmakologi, Fakultas Kedokteran Universitas Brawijaya Malang
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Public Health Veterinary

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Ambon Banana-Tree Sap stimulated Endothelial Cell Migration in Human Umbilical Vein Endothelial Cells (HUVECs) Induced with Inflammatory Mediator IL-1 Wulan, Provisia Marthalita Yuning; Dorothy, Maria Juliana; Alinda, Resha; Sofiana, Kristianingrum Dian; Prihardina, Bunga; Permaasari, Nur; Widodo, M. Aris
Journal of Tropical Life Science Vol 9, No 1 (2019)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (4517.627 KB)

Abstract

Ambon banana-tree (Musa paradisiaca var.sapientum) sap (ABT sap) contains active substances that can stimulate the wound healing process such as: flavonoid, saponin, ascorbic acid. Those substances are needed in an important phase of wound healing called angiogenesis through activation of Vascular Endothelial Growth Factor (VEGF) pathway that stimulates actin reorganization in endothelial cell migration and proliferation. The aim of this study is to evaluate the effectiveness of banana-tree sap in stimulating cells migration, VEGF and nitric oxide (NO) level in Human Umbilical Vein Endothelial Cells (HUVECs) culture that induced by pro-inflammatory cytokine IL-1 (Interleukin-1). The ABT sap was obtained by pressing a fresh Ambon banana stem, then was mixed with culture medium into 0.125%, 0.25% and 0.5% concentration. The ABT sap then was used for treating HUVECs cell that induced by IL-1 200 ng/mL for 48-hours. The result of this study shows that Ambon banana-tree sap increased HUVECs cell migration. However, after 48-hours treatment with ABT sap showed no difference in the VEGF and NO level in the culture media. These results suggested ABT sap able to increase migration to HUVECs that promising for wound healing agent. Therefore, the further research to elucidate the detailed mechanism and identify the active compound warrant to be done.
Effect of Tetrodotoxin from Crude Puffer Fish (Tetraodon fluviatilis) Liver Extract on Intracellular Calcium Level and Apoptosis of HeLa Cell Culture Untario, Natanael; Dewi, Titik Cinthia; Widodo, M. Aris; Rahaju, Pudji
Journal of Tropical Life Science Vol 7, No 1 (2017)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.07.01.04

Abstract

Cervical cancer is the third most commonly diagnosed cancer and fourth leading cause of women death with 8% of total death caused by cancer in women in 2008. Tetrodotoxin (TTX) is a potent neurotoxin found in inner organs puffer fish, with the specific mechanism of sodium channel blocking, and widely used for research purposes. Previous reports claimed that TTX has the capability of inhibiting the metastatic process of cancer and apoptotic effect. Studies also show that apoptosis is a process involving the increase of intracellular calcium level, yet the connection between TTX and increase of intracellular calcium level, therefore triggering apoptosis, has not been established. This is an experimental study with post test only control group design, carried out by exposing HeLa cell culture to a crude liver extract of a puffer fish species, Tetraodon fluviatilis. Crude puffer fish liver extract is administered into HeLa cell culture well in different concentrations 10-4, 10-2, and 10-1. Intracellular calcium level and apoptosis were then measured after 18 hours of incubation. Measurements of intracellular calcium level were done by using CLSM with Fura-2AM staining, and apoptosis by using flowcytometry with Annexin V/PI.  The result shows that there is a significant difference between samples both in intracellular calcium (p < 0.05) and apoptosis (p < 0,05). Both intracellular calcium and apoptosis levels are proportional to liver fish extract concentration. Pearson’s correlation test shows correlation between treatment and intracellular calcium levels (p = 0.000), between treatment and apoptosis (p = 0.002), but not between intracellular calcium and apoptosis (p = 0.05). These results suggest that TTX induces an increase in intracellular calcium level and apoptosis, but calcium pathway is not the sole cause of the apoptosis. 
Co-Authors a, Aulanni'am A, Aulanniam Agus Yuwono Alinda, Resha Amiruddin Amiruddin Arman Sayuti Aulani’ Am Bambang Setiawan Basuki B. Purnomo Dewi, Titik Cinthia Dian Handayani Djoko W. Soeadmadji Dorothy, Maria Juliana Dwinna Aliza Erdiansyah Rahmi HMS Chandra Kusuma Izaak Zoelkarnain Akbar, Izaak Zoelkarnain Muhamad Agus Wibowo Muhammad Hambal Mulyadi Adam, Mulyadi Muslim Akmal Nurdiana Nurdiana Permaasari, Nur Prihardina, Bunga Provisia Marthalita Yuning Wulan Pudji Rahaju, Pudji Sofiana, Kristianingrum Dian Sutiman B. Sumitro Syafruddin s T. Armansyah Tongku Nizwan Siregar Untario, Natanael Yundari, Yundari