The objective of this study was to determine the effect of mindfulness training on female nurses in reducing anxiety. This study had 10 research subjects who were divided into 2 groups, namely 5 subjects for the experimental group (KE) and 5 subjects for the control group (KK). The selection of subjects was carried out randomly. This study used a pre-test post-test control group design. This study used an anxiety scale, FFMQ scale, observation, and interview. Data analysis used Mann Whitney-U to see the difference in anxiety levels in KE and KK. Wilcoxon T-Test was used to see the difference in anxiety levels in KE and KK before and after being given mindfulness training. The results of the analysis showed a difference in anxiety levels in KE and KK with a Z value of -2.312 with a significance of 0.021 (p>0.05). The post-test anxiety score on KE was lower with an average value of 89.8, KK had an average value of 113. Further test results at follow-up in KE and KK showed a significant difference in anxiety levels with a Z value of -2.619 and a significance of 0.009 (p<0.05). The anxiety score at follow-up showed a lower level of anxiety in KE with a mean score of 68.8 while KK had a mean score of 114.4. The T-test showed that there was a difference in anxiety levels at the post-test and follow-up in KE after being given mindfulness training with KK who were not given the treatment. This finding shows that mindfulness training can reduce the anxiety level of female nurses in public hospitals.