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All Journal Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Buletin Agrohorti Jurnal Hortikultura Indonesia (JHI) Jurnal Agrotek Tropika Journal of Tropical Crop Science Menara Perkebunan Jurnal Pusat Inovasi Masyarakat

Ni Made Armini Wiendi

Departemen Agronomi Dan Hortikultura, Fakultas Pertanian, Institut Pertanian Bogor (Bogor Agricultural University), Jl. Meranti, Kampus IPB Darmaga, Bogor 16680, Indonesia

Author-ID : 416879

Agriculture, Biological Sciences & Forestry Economics, Econometrics & Finance Social Sciences

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Variability of Agronomic and Metabolomic Characteristics of Nine Accessions of Cardamom (Amomum compactum) From Central Java, Indonesia Haniefan, Nafarain Agung; Wiendi, Ni Made Armini; Santosa, Edi
Journal of Tropical Crop Science Vol. 12 No. 02 (2025): Journal of Tropical Crop Science
Publisher : Department of Agronomy and Horticulture, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jtcs.12.02.462-472

Amomum compactum (Javanese cardamom) is valued for its unique flavor and aroma, commonly used as a culinary spice or medicinal ingredient. The numerous accessions of cardamom in Indonesia present a significant potential for developing this spice. However, the specific potential of each accession remains to be discovered. This study records the variation of agronomical traits and metabolomic profiles of nine cardamom accessions from Central Java, Indonesia. Multivariate analysis using a heatmap on agronomical traits indicated significant variation among accessions, with clustering based on growth locations. Genetic parameter analysis revealed high phenotypic variability, but varying broad-sense heritability among traits, suggesting the influence of both genotypic and environmental factors. Metabolomic analysis using GC-MS reveals the presence of specific compounds in certain accessions, such as beta-panasinsene in the Kulonprogo Putih accession, 1-docosene and alpha-terpinene in the Banyumas Putih accession, and 9-tricosene in the Banyumas Hybrid, which can serve as markers for these accessions.

Induksi mutasi Stevia rebaudiana dengan perendaman kolkisin secara in vitro (Induced mutation of Stevia rebaudiana through colchicine soaking in vitro) Masna Maya SINTA; Ni Made Armini WIENDI; Syarifah Iis AISYAH
Menara Perkebunan Vol. 86 No. 1 (2018): 86 (1), 2018
Publisher : INDONESIAN OIL PALM RESEARCH INSTITUTE

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/iribb.jur.mp.v1i1.277

Stevia rebaudiana Bert. is a plant producing steviol glycosides that have 200-300 times sweeter than sucrose. These steviol glycosides are produced in the leaves and then spread to all parts of the plant including stems. The use of superior stevia planting material is important for stevia sugar industry. One of the stevia breeding programme is to increase genetic diversity through colchicine soaking to produce polyploid plants. Polyploid plants usually have higher vigor than diploid plants. The purpose of this research was to induce genetic diversity of stevia through colchicine soaking in vitro. Single nodes of sterile stevia clone BS were soaked in colchicine at the concentration of 0.01; 0.02; 0.04; 0.08 and 0.1% for 48 and 72 hours, and in sterile aquadest as a control. Plantlet subcultures were done until MV4 (mutant vegetative 4). Putative mutants were observed by plantlet vigor and stomata analyses on MV5. Vigor of plantlets was observed by counting the number of leaves, nodes, roots, fresh weight and dry weight of the plantlet. Stomata analysis was performed by calculating stomata density, stomata size and chloroplast number in stomata guard cells. Results showed that colchicine soaking treatment increased significantly fresh weight and dry weight of putative mutants. Colchicine soaking treatment increased chloroplast number on stomata guard cell and stomata size, but decreased stomata density. Stevia soaked in colchicine for 48 hours at concentration 0.01-0.04% produce putative mutants with high chromosome numbers. [Key words: poliploidy, stomata, chloroplast, mutant]AbstrakStevia rebaudiana Bert. merupakan tanaman penghasil glikosida steviol yang memiliki tingkat kemanisan 200-300 kali lebih tinggi dibandingkan sukrosa. Glikosida steviol ini diproduksi di daun yang kemudian disalurkan ke bagian tanaman lainnya termasuk batang. Penggunaan klon terbaik stevia merupakan salah satu kunci penting keberhasilan industri gula stevia. Salah satu program pemuliaan tanaman stevia adalah meningkatkan keragaman tanaman melalui mutasi dengan kolkisin sehingga menghasilkan tanaman poliploid. Tanaman poliploid umumnya memiliki vigor lebih baik dibandingkan tanaman diploid. Tujuan dari penelitian ini adalah untuk meningkatkan keragaman stevia melalui peren-daman kolkisin in vitro. Buku tunggal steril stevia klon BS direndam dalam kolkisin dengan konsentrasi 0,01; 0,02; 0,04; 0,08 dan 0,1% selama 48 dan 72 jam dengan perendaman dalam air steril sebagai kontrol. Sub kultur dilakukan hingga MV4 (mutan vegetatif 4). Pengamatan mutan putatif dilakukan meliputi analisis morfologi dan stomata pada MV5. Analisis morfologi dilakukan dengan mengamati jumlah daun, buku, akar, bobot basah serta bobot kering planlet. Analisis stomata dilakukan dengan menghitung kerapatan stomata, ukuran stomata serta jumlah kloroplas pada sel penjaga stomata. Hasil menunjukkan bahwa perendaman stevia pada kolkisin meningkatkan bobot basah serta bobot kering stevia in vitro. Perlakuan perendaman kolkisin meningkatkan jumlah kloroplas pada sel penjaga stomata serta ukuran stomata namun menurunkan kerapatan stomata. Perendaman stevia selama 48 jam pada konsentrasi kolkisin 0,01-0,04% menghasilkan mutan putatif dengan jumlah kromosom tertinggi.[Kata kunci: poliploidi, stomata, kloroplas, mutan]

Increasing Ploidy Level of Garlic (Allium sativum L.) “Tawangmangu Baru” In-Vitro Using Colchicine Hailu, Molla Gebreyohannes; Wiendi, Ni Made Armini; Dinarti, Diny
Journal of Tropical Crop Science Vol. 7 No. 03 (2020): Journal of Tropical Crop Science
Publisher : Department of Agronomy and Horticulture, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jtcs.7.03.122-136

“Tawangmangu Baru” garlic variety is known to have low productivity. The variety is still highly demanded due to its strong flavour and aroma; however, its production has not yet been able to fulfill the local needs of Central Java due to the small size and limited production area. This study aimed to determine the effect of concentration and time duration of colchicine treatment towards increasing the ploidy level of “Tawangmangu Baru” garlic variety for genetic variability. The experimental design used in this study was a complete randomized design with two factorials and 12 combinations. The first factor was concentration of colchicine, i.e. 0.00, 0.02, 0.04, 0.06, 0.08 and 0.10%, and the second factor was the immersion time, i.e. 24 and 48 hours. The result indicated that, 4.72% callus induction was obtained in BDS + 0.4 mg.L-1 2,4-D + 2.0 mg.L-1 kinetin; and 4.0% callus proliferation were obtained in both BDS + 1.5 mg.L-1 2,4-D + 1.0 mg.L-1 kinetin and MS +1.5 mg.L-1 2,4-D and 1.0 mg.L-1 kinetin. The untreated plantlets showed higher mortality rate compared to the explants with 48 hours colchicine treatment. Higher number of shoots were recorded in 0.1% colchicine at 48 hours and lower shoots in 24 hours, whereas 0.1% colchicine at 24 and 48 hours showed the highest ploidy level of total nuclear DNA analyzed by flow cytometry. The genetic diversity of the “Tawangmangu Baru” garlic was successfully enhanced by colchicine and immersion treatment. Mutant lines with tetraploid and mixoploid plants were obtained. The putative lines obtained at 0.1% colchicine treatment were subcultured to produce new mutants before testing the phenotype. The application of colchicine at 24 and 48 hours treatment improved the genetic potential of “Tawangmangu Baru” garlic variety in vitro. The application of colchicine increased the ploidy level and an increase in ploidy is expected to make the bulb size larger. Larger tuber size will increase the tuber weight, and also the overall garlic productivity and production in the future.

Transformasi Genetik Tanaman Kentang cv. Atlantik Dengan Mengintroduksikan Gen Hordothionin untuk Mendapatkan Ketahanan terhadap Penyakit Bakteri Nurhasanah, ,; Wattimena, G. A.; Purwito, Agus; Wiendi, Ni Made Armini; Suharsono, ,
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 31 No. 2 (2003): Buletin Agronomi
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Hordothionins are small anti-bacteria proteins present in barley endosperm, To reveal the potential of this proteins for engineering bacterial disease resistance into potato, a semi-synthetic hordothionin gene construct was introduced in potato cv. Atlantic via Agrobacterium tumefaciens strain LBA 4404, under the control of a cauliflower mosaic virus (CaMV) 358 promotor. The in vitro grown stem (internodus) was used in this research. After 6 weeks in regeneration medium and 2 weeks in rooting medium there were 22 regenerated plants that were screened in kanamycine containing medium. PCR analysis using spesific primer from CaMV 358 promotor showed the presence of amplified T-DNA in 4 transgenic lines from 22 putative transgenic plants were tested, The in vitro toxicity against Ralstonia solanacearum tested from transgenic lines showed variation in resistance level, There were only 2 of the transgenic lines were tolerant, while one of them was moderate tolerant even one of them was susceptible. Key words: Potato, Hordothionin gene, Disease resistance

PERBANYAKAN IN VITRO TANAMAN BAWANG PUTIH (Allium sativum L.) VARIETAS LUMBU PUTIH MELALUI INDUKSI TUNAS ADVENTIF Wiendi, Ni Made Armini; Wattimena, G. A.; Prasetyanti, Enny
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 24 No. 1 (1996): Buletin Agronomi
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Two sets of experiments were conducted to determine the effect of growth hormones, argynine, and coconut water, on the adventitious shoot induction from garlic tissue, and also to find out the best medium for adventitious shoots proliferation. Both experiments could induce direct adventitious shoot and indirect adventitious shoot formation, from calli. Medium with 2 ppm Kinetin and 0.4ppm 2,4-Dproduce good quantity and quality of shoots. The number of shoot from this medium were 32.6 shoots per explant. Medium with 0.5 ppm Kinetin, 0.1 ppm 2.4-D, 25 ppm Argynine, and 10% coconut water produce the highest diameter and good quality of calli, while medium with 1 ppm 2iP and 25 ppm Argynine induced adventitious shoot from calli and produced the highest number of shoot per culture (33.9 shoots).

Pembentukan Embrio Endospermik Sekunder Mangga (Mangifera indica L.) Gedong Gincu Klon 289 Hindaningrum, Irni Furnawanthi; Wiendi, Ni Made Armini; Widodo, Winarso Drajad
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 42 No. 2 (2014): Jurnal agronomi indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

ABSTRACTThe improvement of Mangifera indica L. by conventional breeding approaches has been confounded by the long generation cycle, low fruit set, single seed per fruit and high degree of cross pollination. Biotechnology complements conventional breeding and expedite the mango improvement programs. Endosperm culture is a direct method to produce triploid plants. This study aimed to obtain embryo from endosperm culture. The system of secondary somatic embriogenesis in mango described here represents a source of embryogenic material may be used for mass propagation and genetic manipulation of this crop. The method consisted of induction, proliferation, maturation, germination, and histological analysis of the obtaimed embryos. A protocol for plantlet regeneration was developed for Gedong Gincu mango clone 289 through secondary somatic embryogenesis. Primary somatic embryos (proembryo and cotyledonary embryos) were cultured in induction medium to induce the secondary somatic embryos. The best proliferation rate was 0.22 in medium with 1 g L-1 Poly Vinyl Pyrrolidone (PVP) for multiplication of secondary somatic embryos. Maturation of inoculum derived from the proliferation medium supplemented with 2 g L-1 of activated charcoal on medium containing 0.4 mg L-1 BAP provides the average 2.39 embryo formation of cotyledonari phase. The highest germination frequency (20%) was obtained in media with GA3 1.5 mg L-1.Keywords: endosperm, Gedong Gincu, Mangifera indica L, secondary endospermic embrio

Deteksi Kestabilan Genetik Ramet Kelapa Sawit Hasil Kultur In Vitro Menggunakan SSR Cahyaningsih, Yuni Fitri; Wiendi, Ni Made Armini; Toruan-Mathius, dan Nurita
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 44 No. 1 (2016): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

ABSTRACTCommercial production of oil palm ramet requires the guarantee of high genetic stability. The objectives of this research were to determine 1) genetic diversity of ortet as source of explant, and 2) genetic stability of ramet derived from ortet propagated through tissue culture. Genetic stability analysis was done using ramet from five Tenera (D×P) oil palm ortets.As many as 20 ramets were randomly chosen from each ortet. A total of 100 ramets were used for genetic stability analysis. Genetic similarity analysis was analyzed using NTSyspc version 2.1 software with method Similarity for Qualitative Data and Unweighted Pair Group Method Aritmatic (UPGMA). The results indicated 20 SSR primer pairs were polymorphic and could form 44 alleles. As many as 80% of ramets from IS 3 ortet showed genetic similarity ranged from 97-100% to the ortet. All ramets derived from IS 10, IS 20 and IS 40 ortet had 90-100% of genetic similarity to its respective ortet. Futhermore, 95% of ramets from IS 39 ortet had 97-100% of genetic similarity to the ortet.Keywords: Elaeis guineensis Jacq., genetic similarity, tissue culture

Keragaman Genetik Kacang Bogor (Vigna subterranea L. Verdc.) Berdasarkan Marka SSR (Simple Sequence Repeat) Illahi, Zikril; Wiendi, Ni Made Armini; Sudarsono, dan
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 44 No. 3 (2016): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

ABSTRACTBambara groundnut (Vigna subterranea L. Verdc.) is an important underutilized legume crop in Indonesia. The aim of this research is to study genetic diversity of bambara groundnut from Sukabumi and Sumedang, West Java, Indonesia. This study used 107 bambara groundnut accessions, which consisted of 57 accessions from Sukabumi and 50 accessions from Sumedang. We use five simple sequence repeat (SSR) to analyze the accessions. Totally nine alleles were detected, with a mean of 1.8 alleles per locus. Allelic and gene diversities were higher in Sumedang (1.8 alleles per locus and 0.119) than in Sukabumi population (1.4 alleles per locus and 0.020), respectively. We constructed a phylogenic tree by Neighbor-Joining analysis based on genetic distances (DA) and showed the tree divided bambara groundnut accessions into two broad groups according to the origin of samples (Sukabumi and Sumedang). Results from the phylogenic tree are in line with those from the population structure analysis. Keywords: allele diversity, bambara groundnut, genetic distance, under utilized legume

Perbanyakan In Vitro Dendrobium Indonesia Raya ‘Ina’ melalui Embriogenesis Somatik Berbasis Sistem Bioreaktor Rachmawati, Fitri; Wiendi, Ni Made Armini; Mattjik, Nurhajati Ansori; Purwito, Agus; Winarto, dan Budi
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 44 No. 3 (2016): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

ABSTRACTAn effective and efficient in vitro propagation system has important roles in preparing and producing high quality-seedlings of Dendrobium for commercial scale. The objective of this research was to establish an effective and efficient embryogenic callus (EC) proliferation method using bioreactor system and regeneration EC into plantlet for producing high quality seedlings of Dendrobium Indonesia Raya ‘Ina’. Differences in callus densities (5, 10, 15, and 20 g callus in 250 mL medium), aeration levels (2.5, 5.0, and 10.0 O2 volume per medium volume per minute; vvm), and regeneration media half-strength MS and 2 g L-1 NPK (32:10:10) combinated by 0.00, 0.05 mg L-1 BA, 150 mL L-1 coconut water and their combinations were tested in this experiment. The experiments were arranged using randomized completely block design (RCBD) with three replications for EC proliferation and randomized completely desaign (RBD) for EC regeneration. The results showed that combination of aeration at 2.5 vvm and 10 g of EC was the most suitable aeration level and callus density for proliferation of EC in the 500 ml airlift bioreactor with 6.85 multiplication rate, 92.5% EC formation, and malformed callus morphology as low as 6.1%. The highest somatic embryos (SEs) formation was 87.7% with 44.5 SEs per clump and 92.1% SEs germination with 41.0 germinated-SEs per clump, 85.1% normal germinated-SEs, and whereas the best performance of plantlet was obtained from 1/2 MS + 0.05 mg L-1 BA semi solid medium. Plantlets were successfully acclimatized using Cycas rumphii medium with high survival rate (91.6%). Keywords: aerations, callus densities, germination, media, somatic embryos

Proliferation of porang (Amorphophallus muelleri Blume) from bulbils and leaf cutting treated by NAA and BA Putri, Ayu Diah Putu Laksmi; Wiendi, Ni Made Armini; Santosa, Edi
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 51 No. 3 (2023): Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy)
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24831/jai.v51i3.48958

Javanese konjac or iles-iles (Amorphophallus muelleri Blume, Araceae) is a tuber crop native to Indonesia as a source of carbohydrat, also contains a lot of glucomannan, which has high economic value as a raw material in industry. The study aimed to develop the propagation method of A. muelleri from bulbils and leaf cuttings through the plant growth regulator (PGR) application of α-naphthaleneacetic acid (NAA) and benziladenin (BA). The research was conducted from August 2020 to December 2021 at IPB University, Bogor. The study consisted of three experiments based on the type of propagation material. Each experiment used a randomized complete block design with three factors, i.e., NAA concentrations (0, 2, and 4 mg L-1), BA concentrations (0, 5, 10, and 15 mg L-1), and propagation materials (Experiment 1: small, medium, large bulbils; Experiment 2: immature and mature peak leaflet cuttings; Experiment 3: immature and mature base leaflet cuttings). The results showed that there was an interaction of three factors in the bulbils and PGR application, which had a significant effect on the percentage of axillary shoot proliferation and growth. In Experiment 2, PGR application was unable to regenerate peak leaflet cuttings, whereas in Experiment 3 PGR encouraged base leaflet cuttings to form shoots, roots, and bulbils, especially at concentrations of 2 mg L-1 NAA+15 mg L-1 BA and 4 mg L-1 NAA+15 mg L-1 BA. Keywords: α-naphthaleneacetic acid, benziladenin, iles-iles, Javanese konjac, plant growth regulators

Co-Authors , Krisantini , Nurhasanah , Suharsono Agus Purwito Ajisyahputra, Nikko Rizky Amelia Paramitha Mahanani Awang Maharijaya Bagus Setyo Yudhanto Baidowi, Muhammad Bawonoadi, Gilar Cahyaningsih, Yuni Fitri Constantin, Mondjeli Didy Sopandie Diny Dinarti Edi Santosa Edi Santosa Edi Santosa Endah Retno Palupi Enny Prasetyanti Eny Widajati Fitri Rachmawati Flowrentyka Ferona Haloho G. A. Wattimena Gilar Bawonoadi Hailu, Molla Gebreyohannes Haniefan, Nafarain Agung I Gusti Ngurah Galang Aditya Illahi, Zikril Indah Permata Dewi Irni Furnawanthi Hindaningrum Joko Mulyono Juang Gema Kartika Khalim Rizkiana Bahri Lailatul Qodriyah Agne Verawati Masna Maya SINTA Megayani Sri Rahayu Muhammad Baidowi Musalamah Nurhajati Ansori Mattjik PUTRI, AYU DIAH PUTU LAKSMI Putri, Devina Daratyama Rahmah, Syifaur Rai, Sonya Putri Ratna Trisnawati Sobir Sobir Sonya Putri Rai Sri Rianawati Sudarsono, dan Sugiyanta Syarifah Iis Aisyah Toruan-Mathius, dan Nurita Trikoesoemaningtyas Tubagus Kiki Kawakibi Azmi Ulil Azmi Nurlaili Afifah Wachyar, Ade Willy Bayuardi Suwarno Winarso D. Widodo Winarto, dan Budi Yudhanto, Bagus Setyo

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