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All Journal HAYATI Journal of Biosciences MANAJEMEN HUTAN TROPIKA Journal of Tropical Forest Management MEDIA KONSERVASI Indonesian Journal of Geography REKA GEOMATIKA Geoplanning : Journal of Geomatics and Planning IJOG : Indonesian Journal on Geoscience Journal of Degraded and Mining Lands Management Indonesian Journal of Geospatial Journal of Mathematical and Fundamental Sciences Journal of Engineering and Technological Sciences Indonesian Journal of Biotechnology Forum Geografi Indonesian Journal of Science and Technology JURNAL ILMIAH GEOMATIKA Jurnal Penginderaan Jauh dan Pengolahan Data Citra Digital International Journal of Remote Sensing and Earth Sciences (IJReSES) JURNAL GEOGRAFI Geografi dan Pengajarannya Indonesian Journal of Electrical Engineering and Computer Science Indonesian Journal on Geoscience Indonesian Journal on Geoscience Bulletin of Geology Geosfera Indonesia Jurnal Penginderaan Jauh dan Pengolahan Data Citra Digital
Ketut Wikantika
Centre for Remote Sensing, Bandung Institute of Technology (ITB) and Faculty of Earth Sciences and Technology, Bandung Institute of Technology (ITB)
Aerospace Engineering Agriculture, Biological Sciences & Forestry Astronomy Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Control & Systems Engineering Earth & Planetary Sciences Education Electrical & Electronics Engineering Energy Engineering Environmental Science Immunology & microbiology Materials Science & Nanotechnology Mathematics Physics Social Sciences

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Journal : Indonesian Journal of Biotechnology

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Phylogenetic analysis of 23 accessions of Indonesian banana cultivars based on Internal Transcribed Spacer 2 (ITS2) region Karlia Meitha; Intan Fatmawati; Fenny Martha Dwivany; Agus Sutanto; Sigit Nur Pratama; Husna Nugrahapraja; Ketut Wikantika
Indonesian Journal of Biotechnology Vol 25, No 1 (2020)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijbiotech.49506

Abstract

Pisang Kepok (Musa spp. [ABB ’Saba’ subgroup]) has several unique characteristics, such as tolerance to drought and Fusarium Foc (TR4) disease. Currently, the genetic diversity of Pisang Kepok in Indonesia is not well identified, although it is widely cultivated. Information on genetic diversity is essential for developing breeding strategies to achieve efficient cultivar improvement in the future. Aims of this research were to analyze the genetic variation of Pisang Kepok from some islands in Indonesia and to determine the genetic relationship between Pisang Kepok and other accessions banana cultivars based on ITS2 region, as a basis for future research in improving banana quality through molecular breeding. We have conducted the multiple sequence alignment and built the phylogenetic tree analysis using the Bayesian Inference Phylogeny method of one million generations (ngen = 1,000,000). The ITS2 region showed two clade ingroups: first clade consists of banana with B genome (balbisiana), while the second clade consists of banana with only A genome (acuminata). In general, all accessions of Pisang Kepok cultivars were clustered in the B genome of bananas cultivars. In addition, the ITS2 sequences and secondary structures among Pisang Kepok from various regions are identical, suggesting that there was no genetic variation in the ITS2 region of Pisang Kepok from multiple areas in Indonesia.
Co-Authors Abd. Rasyid Syamsuri Adhi Wibowo Adriana Hiariej, Adriana Agung B. Harto Agung Budi Harto Agung Budi Harto Agung Budi Harto Agung Budi Harto Agung Budi Harto Agung Budi Harto Agung Budi Harto Agung Budi Harto Agung Budi Harto Agung Budi Harto Agus Handoyo Harsolumakso Agus Handoyo Harsolumakso, Agus Handoyo Agus Sutanto Agus Sutanto Ahmad Luthfi Hadiyanto Akihiko Kondoh Aldyansyah, Muhammad Aminah Kastuari Anesta, Aqilla Fitdhea Anggun Tridawati Aqilla Fitdhea Anesta Armi Susandi Armi Susandi Ary Setijadi Prihatmanto Asep Saepuloh Asep Saepuloh Asep Yusup Saptari Asep Yusup Saptari, Asep Yusup Asmi M. Napitu Asmi M. Napitu Aswin Rahadian Bambang Widarsono Bobby S. Dipokusumo Dandy A. Novresiandi Darmawan S Darmawan S, Darmawan Dedi Irawadi Deni Suwardi Desti Ayunda Dudung M Hakim Dudung Muhally Hakim Dudung Muhally Hakim Fahmi, Muhammad Nurul Farah Nafisa Ariadji Fauziah, Afi Fenny M. Dwivany FENNY MARTHA DWIVANY Ghazali, Mochamad Firman Ghozali, M. Firman Giasintha Stefani Golok Jaya, La Ode Muhammad Hary Nugroho Herru Lastiadi Setiawan Himasari Hanan Husna Nugrahapraja I Nyoman Dibia I NYOMAN RAI I Wayan Nuarsa Imam A. Sadisun Intan Fatmawati Irland Fardani Ishak H. Ismullah Jevon A. Telaumbanua Karlia Meitha Katmoko Ari Sambodo Katmoko Ari Sambodo, Katmoko Ari LILIK BUDIPRASETYO Lissa F. Yayusman Luky Adrianto Lumbantobing, Marlonroi Mamad Sugandi Marlonroi Lumbantobing Mila Olivia Trianaputri Mirelva, Prima Rizky Mochamad Firman Ghazali Mochamad Firman Ghazali Nengah Widiadnyana Nengah Widiadnyana Nisrina Sukriandi Nurjanna Joko Trilaksono Prihanggo, Maundri Prila Ayu Dwi Prastiwi Purnama, Yustika Retno Dammayatri Rian Nurtyawan Riantini Virtriana S. Suliantara Satria Bijaksana Shafarina Wahyu Trisyanti Sigit Nur Pratama Simarmata, nirmawana Soni Darmawan Sony Darmawan, Sony Sugandi, Mamad Sukristiyanti Sukristiyanti Supriadi A Supriadi A, Supriadi Susantoro, Tri Muji Suwardhi, Deni Tahjudil Witra Tan, Alex Tohir, Rizki Kurnia Tombayu A. Hidayat Topik Hidayat Tri Muji Susantoro Tri Muji Susantoro Tri Muji Susantoro Tri Muji Susantoro Tri Muji Susantoro Tri Muji Susantoro, Tri Muji Trianaputri, Mila Olivia Tridawati, Anggun Trika Agnestasia Tarigan Yayusman, Lissa Fajri Yudi Setiawan