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Nanang Yunarto
Center For Biomedical And Basic Technology Of Health, Ministry Of Health
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Decision Sciences, Operations Research & Management Earth & Planetary Sciences Energy Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Nursing Public Health

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Determination of HPLC Chromatogram Profile of Katuk (Breynia androgyna (L.) Chakrab. & N.P.Balakr) Plants from Ristoja's Results using Chemometric Analysis Yunarto, Nanang; Alegantina, Sukmayati; Isnawati, Ani
Jurnal Kefarmasian Indonesia VOLUME 12, NOMOR 2, AGUSTUS 2022
Publisher : Pusat Penelitian dan Pengembangan Biomedis dan Teknologi Dasar Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22435/jki.v0i0.6040

Abstract

The katuk plant was known as Sauropus androgynous (L.) Merr, but the name is changed to Breynia androgyna (L.) Chakrab. & N.P. Balakr since the publication of Chakrab's & N.P. Balakr in the 2012 Journal of Plant Taxonomists. The content of chemical compounds and secondary metabolites of katuk leaf are strongly influenced by different habitat or location. Therefore, the Center for Research and Development of Medicinal Plants and Traditional Medicines, National Institute of Health Research and Development (NIHRD) has conducted Research on medicinal plants and herbs (RISTOJA) results on 58 samples of katuk plants originating from 7 provinces and used by 13 ethnic groups in Indonesia. This study aims to obtain plant quality based on the description of the High-Performance Liquid Chromatography (HPLC) chromatogram profile, in order to obtain the suitability of the efficacy of medicinal plants with their benefits for the community in an area. Test using HPLC with a gradient mobile phase, mixture of acetonitrile and methanol for 60 minutes. The results of the chromatogram were analyzed chemometrically by using Principal Component Analysis (PCA) data interpretation. PCA results showed that from HPLC chromatograms at 254 nm and 366 nm, each gave 3 different clusters, namely Clusters A, B and C where each cluster has the same chromatogram profile of katuk plants. Cluster A which was identified at a wavelength of 254 nm was the most used cluster by 7 ethnic groups with a total of 28 Katuk samples. Similarly, the chromatogram at a wavelength of 366 nm with the largest cluster is cluster A which is used by 11 ethnic groups with a total of 45 samples of katuk plants. where each cluster had the same chromatogram profile of katuk Plants.
Antioxidant and Inhibition Lipase Enzyme Activity of Centella asiatica Leaf Extract Yunarto, Nanang; Mawadatun, Ummi; Reswandaru, Uud Nourma; Sopian, Ahmad; Isnawati, Ani; Alegantina, Sukmayati
Jurnal Kefarmasian Indonesia VOLUME 13, NUMBER 2, AUGUST 2023
Publisher : Pusat Penelitian dan Pengembangan Biomedis dan Teknologi Dasar Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22435/jki.v13i2.6609

Abstract

Hyperlipidemia and many other metabolic diseases are related to oxidative stress. Centella asiatica is a herbal medicine with reported antioxidant effect in vitro. Centella asiatica contains secondary metabolites asiaticoside which are included in the terpenoid group. The study evaluated the respective antioxidant potential and lipase enzyme inhibition of Centella asiatica leaves extract (CAE). Centella asiatica were extracted in ethanol, and the extract was assayed for the measurement of asiaticoside. Ethanolic extracts of asiaticoside content were prepared for HPLC analysis The antioxidant potential of extracts was assessed by its free radical scavenging activity such as 2, 2-diphenyl -1-picrylhydrazyl as well as reducing. The anti-hyperlipidemic effect was evaluated in vitro lipase inhibitory activity test carried out enzymatically using the ELISA method with simvastatin as a comparison. The results showed asiaticoside contain in CAE 1.26%; the IC50 value of the antioxidant test of CAE was 11.38 g/mL; the IC50 value of the lipase enzyme in the CAE was 26.14 g/mL. The antioxidant activity of CAE is categorized as very strong and has the potential to inhibit lipase enzymes. The study suggests that CAE has the potential to inhibit lipase activity, suppressing lipid digestion and thereby diminishing entry of lipids into the body.  
In vivo Study of Uric Acid Inhibitory of Catechin from Gambir [Uncaria gambir (Hunter) Roxb] in Male Rats Yunarto, Nanang; Ismaya, Nurwulan Adi; Reswandaru, Uud Nourma; Hermiyanti, Evika; Gumilang, Nabila Cahya; Salina, Fitri
Jurnal Kefarmasian Indonesia VOLUME 14, NUMBER 1, FEBRUARY 2024
Publisher : Pusat Penelitian dan Pengembangan Biomedis dan Teknologi Dasar Kesehatan

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Abstract

Hyperuricemia is a metabolic disease characterized by high levels of uric acid in the blood. In silico studies of catechin compounds using molecular docking were able to inhibit the activity of the xanthin oxidase enzyme so that it could reduce uric acid levels. One of the native Indonesian plants that contains catechin is gambier (Uncaria gambir (Hunter) Roxb). This research aims to obtain the efficacy and safety of gambier leaf catechins in reducing uric acid levels. In vivo efficacy testing at doses of 20, 40, and 40 mg/Kg BW with allopurinol as a comparison and the safety of gambier leaf catechin in hyperuricemic male mice through macroscopic and microscopic examination of the liver and kidney organs. The results of research on determining catechin levels using HPTLC obtained levels of 96.06%. Gambier leaf catechin at doses of 20, 40, and 40 mg/Kg BW was able to reduce uric acid levels in the blood serum of mice. The higher the dose of catechin, the better the anti-hyperuricemia effect. In the results of the safety parameters, gambier leaf catechins, there were no specific abnormalities in the liver and kidneys of mice both macroscopically and microscopically. Based on research, it can be concluded that gambier leaf catechin has great potential as an anti-hyperuricemia drug and has good safety if consumed for a long time.
Cytotoxic Activities of Bioactive Fraction of Uncaria gambir (Hunter) Roxb on MCF-7 Human Breast Cancer Cell Line Yunarto, Nanang; Fadhilah, Humaira; Purnamasari, Rini; Reswandaru, Uud Nourma
Jurnal Kefarmasian Indonesia VOLUME 15, NUMBER 1, FEBRUARY 2025
Publisher : Pusat Penelitian dan Pengembangan Biomedis dan Teknologi Dasar Kesehatan

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Abstract

Breast cancer is a disease with the highest mortality rate. The first line of breast cancer treatment uses chemotherapy, but it has serious side effects on patients. Gambir (Uncaria gambir (Hunter) Roxb.) contains catechin compounds that show cytotoxic activity. This study aims to evaluate the antioxidant activity and cytotoxic effects on MCF-7 human breast cancer cells from the bioactive fraction of gambir leaves. Gambir extract was fractionated with water, ethyl acetate, and n-hexane solvents. Determination of catechin levels in the fractions used high-performance thin-layer chromatography. Antioxidant activity was tested using 2,2'-diphenyl-1-picrylhydrazyl (DPPH) and cytotoxicity tests on MCF-7 cells were carried out using MTT assay with inhibition of protein expression anti-apoptosis was determined by immunocytochemistry assay. The ethyl acetate fraction had the highest catechin content, namely 93.05%. The results of the antioxidant activity test showed that the IC50 values were consecutively for the water fraction 42.48 µg/mL, ethyl acetate 14.86 µg/mL, and n-hexane 49.63 µg/mL. In the MTT test, the ethyl acetate fraction had more potent cytotoxic activity with an IC50 value of 87.52 µg/mL. This study shows that the ethyl acetate fraction of gambir leaves has potent antioxidant and cytotoxic activity. The higher catechin content relates to antioxidant and cytotoxic activity against MCF-7 cells.
EVALUASI FISIK FORMULASI SEDIAAN OBAT KUMUR YANG MENGANDUNG EKSTRAK BUAH STROBERI DAN DAUN PEPPERMINT Sopian, Ahmad Sopian; Yunarto, Nanang; Rahimah, Vina Nasriani
PHRASE (Pharmaceutical Science) Journal Vol 5, No 2 (2025): Pharmaceutical Science Journal Vol 5 No 2, 2025
Publisher : Sekolah Tinggi Ilmu Kesehatan Widya Dharma Husada Tangerang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52031/phrase.v5i2.1176

Abstract

ABSTRAKWorld Health Organization (WHO) 2022 memperkirakan penyakit mulut mempengaruhi hampir 3,5 miliar orang diseluruh dunia. Berdasarkan Data RISKESDAS 2018 menunjukkan sebanyak 45% responden mengalami masalah gigi berlubang, 19% masalah gigi hilang atau tanggal sendiri, 10,4% kasus gigi goyah, dan 4,1% gigi ditambal. Data ini menunjukkan bahwa kesadaran masyarakat untuk memeriksa kesehatan gigi dan mulut masih kurang. Sehingga obat kumur dapat digunakan sebagai pencegahan atau pengobatan infeksi tenggorokan. Tujuan penelitian ini untuk mengetahui uji viskositas dan uji stabilitas fisik pada formulasi obat kumur ekstrak buah stroberi dan daun peppermint. Metode penelitian yang digunakan metode penelitian eksperimental. Hasil Penelitian yang diperoleh pada uji viskositas mendapatkan hasil rata-rata yaitu pada formulasi F0 (0%) 3,944 cp, F1 (5%) 4,016 cp, F2 (15%) 4,96 cp dan F3 (25%) 6,412 cp. Uji stabilitas fisik pada formulasi obat kumur ekstrak buah stroberi dan daun peppermint terdiri dari uji organoleptis, uji pH, uji kejernihan, dan uji homogenitas. Dari hasil yang didapatkan menunjukkan bahwa sediaan tidak stabil dan tidak memenuhi syarat standar obat kumur.
Co-Authors Ade Nugraheni, Ade Ahmad Sopian, Ahmad Aini Nurul Aini, Nurul Ani Isnawati Arifayu Addiena Kurniatri Arifayu Addiena Kurniatri Arifayu Addiena Kurniatri Arifin, Kelik M Berna Elya Dadang Suherman DIAH PERMATA SARI, DIAH PERMATA Furqon, Ainun Gumilang, Nabila Cahya Handayani, Aisyah Hanief Mulia Ar Rossyid Hermiyanti, Evika Herni Asih Setyorini Humaira Fadhilah Indah Sulistyowati Indah Sulistyowati Indah Sulistyowati, Indah Indhira Oliffia Prameswari Intan Sari Oktoberia Intan Sari Oktoberia Kurniatri, Arifayu Addiena Lailaty, Intani Quarta Lisa Andriani Lienggonegoro Mawadatun, Ummi Mihardja, Laurentia K. Novi Sulistyaningrum Novi Sulistyaningrum Nurul Aini Nurwulan Adi Ismaya Qotrunnada Linggar Pinanditi Rahimah, Vina Nasriani Reswandaru, Uud Nourma Rini Purnamasari, Rini Rosa Adelina Rosyidah, A'liyatur Salina, Fitri Sari, Dewi Ratih Tirto Sheila Meitania Utami Sopian, Ahmad Sopian Sukmayati Alegantina Sulistyowati, Indah Tri Madesa Patadungan Tri Okta Ratnaningtyas Uud Nourma Reswandaru