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All Journal Dentika Dental Journal Althea Medical Journal Journal of Dentomaxillofacial Science Medicinus : Jurnal Kedokteran Majalah Anestesia dan Critical Care Jurnal Integrasi Kesehatan dan Sains Jurnal Ilmu Faal Olahraga Indonesia Jurnal Riset Kedokteran Bandung Conference Series : Medical Science

Hendro Sudjono Yuwono

Fakultas Kedokteran Universitas Islam Bandung

Author-ID : 426799

Humanities Biochemistry, Genetics & Molecular Biology Dentistry Health Professions Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Nursing Public Health

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Effects of Calcium Bentonite on High Blood Cholesterol Level Hendro Sudjono Yuwono; Fadhli Rajif Tangke; Reni Farenia
Althea Medical Journal Vol 5, No 3 (2018)
Publisher : Faculty of Medicine Universitas Padjadjaran

Background: Calcium bentonite has been known to decrease high blood cholesterol level. This study aimed to explore whether a local calcium bentonite from West Java, Indonesia, had effect on lowering total blood cholesterol level.Methods: A laboratory experimental study was carried out on thirty adult male Wistar rats’ at Laboratory animals, Department of Pharmacology and Therapy, Universitas Padjadjaran in 2012. The rats were divided into five groups with six rats in each group, consisting of group I (negative control) with standard diet and distilled water, group II (positive control) with high lipid diet and distilled water, group III with high lipid diet and ezetimibe 0.18 g/rat/day, groups IV with high lipid diet and calcium bentonite 0.25 g/rat/day, group V with high lipid diet and calcium bentonite 0.5 g/rat/day. Ezetimibe as was used as this was an inhibitory agent of intestinal cholesterol absorption. Blood cholesterol levels were measured and analyzed using analysis of variance and Tukey’s post-hoc test.Results: Oral calcium bentonite at 0.25 g/rat/day and 0.5 g/rat/day significantly decrease total blood cholesterol level relative to the positive control group, 31.68% (p=0.018) and 32.87% (p=0.006) respectively, but its effect is inferior to ezetimibe 62.83% (p=0.000).Conclusions: Local calcium bentonite from West Java, Indonesia, has a significant lowering effect, however, the effect is less comparing to ezetimibe.

Aktivitas Antibakteri Ekstrak Air Kopi Robusta (Coffea Canephora) terhadap Bakteri Pseudomonas aeruginosa Naura Mufida Marsya; Hendro Sudjono Yuwono; Oky Haribudiman
Jurnal Integrasi Kesehatan dan Sains Vol 3, No 1 (2021): Jurnal Integrasi Kesehatan dan Sains
Publisher : Universitas Islam Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29313/jiks.v3i1.7288

Pseudomonas aeruginosa umum ditemukan pada Healthcare-associated infections (HAIs) dan menunjukkan resistensi pada banyak agen antimikrob yang umum. Kopi Robusta telah dipilih sebagai antibiotik alternatif karena memiliki kemampuan antibakteri. Penelitian ini bertujuan mengetahui aktivitas antibakteri ekstrak air kopi robusta (Coffea canephora) terhadap bakteri Pseudomonas aeruginosa. Penelitian dilakukan di Laboratorium Mikrobiologi dan Parasitologi Fakultas Kedokteran UNPAD periode Maret – Desember 2020. Perlakuan yang diberikan adalah media agar diberi disk yang mengandung ekstrak air kopi robusta dalam 4 dosis, yaitu 12,5%, 25%, 50%, dan 100%, imipenem (kontrol positif), dan disk yang mengandung aquadest (kontrol negatif). Agar diinkubasi dalam inkubator dengan suhu 37ºC selama 24 jam. Penilaian dilakukan dengan pengamatan dan pengukuran zona inhibisi. Data hasil penelitian membuktikan zona inhibisi yang dihasilkan oleh ekstrak air kopi robusta pada dosis 100% memiliki rerata paling tinggi dibanding dengan dosis lainnya, yaitu 8,13 mm, namun lebih kecil dibanding dengan imipenem yang memiliki rerata zona inhibisi sebesar 25 mm. Hal ini menunjukkan bahwa ekstrak air kopi robusta memiliki efek antibakteri terhadap Pseudomonas aeruginosa. Antibacterial Activities of Robusta Coffee Water Extract (Coffea Canephora) against Pseudomonas aeruginosa BacteriaPseudomonas aeruginosa is common in Healthcare-associated infections (HAIs) and shows resistance to many common antimicrobial agents. Robusta coffee has been chosen as an alternative to antibiotics because of its antibacterial properties. This study aims to determine the antibacterial activity of Robusta coffee (Coffea canephora) water extract against Pseudomonas aeruginosa. The research was done in Laboratorium Mikrobiologi and Parasitologi Fakultas Kedokteran UNPAD period March – December 2020.The treatments given was agar plate that added Robusta coffee water extract (Coffea canephora) in 4 doses, namely 12.5%, 25%, 50%, and 100%, imipenem antibiotic disc (positive control), and disk containing aquadest (negative control). They were then incubated in an incubator at 37ºC for 24 hours. The assessment was carried out by observing and measuring the inhibition zone. The research data proved that the inhibition zone produced by robusta coffee water extract at a dose of 100% had the highest average compared to other doses, which is at 8.13 mm, but smaller than imipenem which had an average inhibition zone of 25 mm. This shows that robusta coffee water extract has an antibacterial effect against Pseudomonas aeruginosa.

EFEK ANTIBAKTERI VIRGIN COCONUT OIL TERHADAP METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS Tirta Darmawan Susanto; Muchtan Sujatno; Hendro Sudjono Yuwono
Medicinus Vol 4, No 8 (2015): February 2015 - May 2015
Publisher : Fakultas Kedokteran Universitas Pelita Harapan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19166/med.v4i8.1186

Kejadian infeksi, baik di lingkungan rumah sakit yang disebut infeksi nosokomial maupun di luar rumah sakit, cukup besar. Infeksi mempertinggi angka kematian dan kesakitan, serta memperlama waktu perawatan di rumah sakit. Saat ini banyak tanaman yang terbukti secara empiris memiliki efek antibakteri, diantaranya adalah minyak kelapa murni (Virgin Coconut Oil / VCO), oleh karena itu perlu dilakukan penelitian ini guna mengetahui efek anti bakteri VCO pada luka yang telah diinfeksi dengan Methicillin Resistant Staphylococcus aureus (MRSA).Telah dilakukan penelitian eksperimental laboratorium dengan desain penelitian Rancangan Acak Lengkap. Hewan coba yang digunakan adalah marmut jantan galur albino sebanyak 15 ekor . Dermis hewan coba diinsisi hingga diperoleh luka berukuran 9cm2 dasar otot, luka diinfeksi dengan suspensi bakteri MRSA, kemudian diberi 3 macam perlakuan yaitu dibiarkan saja tanpa pengobatan untuk control negatif, terapi VCO per oral 3 cc / hari dan terapi VCO topikal 0,4 cc / hari. Data yang diperoleh dianalisis dengan analysis of variance (ANOVA) dan two sample – T test.Hasil penelitian menunjukkan bahwa pemberian VCO topikal dengan dosis 0,4 cc / hari mampu mengobati infeksi dan mempercepat penyembuhan luka hewan coba secara bermakna (p = 0,043 < α = 0,05), tetapi pemberian VCO peroral 3 cc / hari menunjukkan hasil yang tidak bermakna (p = 0,376 > α = 0,05). Pemberian VCO per oral 3 cc / hari dan topikal 0,4 cc / hari mampu menurunkan jumlah lekosit darah pada hewan coba. Pemberian VCO topikal 0,4 cc / hari mampu membunuh bakteri sehingga dapat mengurangi jumlah bakteri pada luka secara bermakna (p = 0,0092 < α = 0,05), namun pemberian VCO per oral tidak mampu mengurangi jumlah bakteri pada luka secara bermakna (p = 0,17 > α = 0,05). Pada hasil penelitian di atas, dapat disimpulkan bahwa pemberian VCO topikal menunjukkan efek antibakteri yang bermakna untuk mengatasiinfeksi MRSA.

Penatalaksanaan ludwigâ€™s angina pada anak Management of ludwigâ€™s angina at child Eddy Hermanto; Abel Tasman Yuza; Hendro Sudjono Yuwono
Journal of Dentomaxillofacial Science Vol. 10 No. 1 (2011): Formerly Jurnal Dentofasial ISSN 1412-8926
Publisher : DiscoverSys Inc.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15562/jdmfs.v10i1.255

Ludwigâ€™s angina is an acute progressive cellulitis that involves the submental and submandibular spaces, bilateralsublingual space, which is caused by the Streptococci bacteria. Characteristics of this disease are swelling of thebilateral soft tissue submandibular, submental region and floor of the mouth, and elevation of the tongue. Earlydiagnosis and management of infection in children is important because a child has tendency for a progressiveworsening and lead to systemic infection. Infections involving the facial regions in children are rare cases wherebyonly one case is found in the last two years at the oral surgery department. This report shows a case of a seven-yearold girl with Ludwigâ€™s angina that came to the emergency division of the oral surgery department with a chiefcomplaint of swelling at the chin and both cheeks. The complaint was first known as caries at 36, 74, and 75.Treatment includes antibiotic administration, incision and drainage, and extraction of the causative tooth. Patientwas hospitalized and returned home when was in good condition.

Aktivitas Antibakteri Ekstrak Air Kopi Robusta (Coffea canephora) terhadap Bakteri Pseudomonas Aeruginosa Naura Mufida Marsya; Hendro Sudjono Yuwono
Jurnal Riset Kedokteran Volume 1, No.1, Juli 2021, Jurnal Riset Kedokteran (JRK)
Publisher : UPT Publikasi Ilmiah Unisba

Abstract. Pseudomonas aeruginosa is common in Healthcare-Associated Infections (HAIs) and shows resistance to many common antimicrobial agents. Robusta coffee has been chosen as an alternative to antibiotics because of its antibacterial properties. This study aims to determine the antibacterial activity of Robusta coffee (Coffea canephora) water extract against Pseudomonas aeruginosa. The treatments given was agar plate that added Robusta coffee water extract in 4 doses, namely 12.5%, 25%, 50%, and 100%, imipenem antibiotic disc (positive control), and disk containing aquadest (negative control). They were then incubated in an incubator at 37ºC for 24 hours. The assessment was carried out by observing and measuring the inhibition zone. The research data proved that the inhibition zone produced by robusta coffee water extract at a dose of 100% had the highest average compared to other doses, which is at 8.13 mm, but smaller than imipenem which had an average inhibition zone of 25 mm. This shows that robusta coffee water extract has an antibacterial effect against Pseudomonas aeruginosa. Abstrak. Pseudomonas aeruginosa umum ditemukan pada Healthcare-Associated Infections (HAIs) dan menunjukkan resistensi pada banyak agen antimikroba yang umum. Kopi Robusta telah dipilih sebagai antibiotik alternatif karena memiliki kemampuan antibakteri. Penelitian ini bertujuan untuk mengetahui aktivitas antibakteri ekstrak air kopi robusta (Coffea canephora) terhadap bakteri Pseudomonas aeruginosa. Perlakuan yang diberikan adalah media agar diberi disk yang mengandung ekstrak air kopi robusta dalam 4 dosis yaitu 12,5%, 25%, 50%, dan 100%, imipenem (kontrol positif), dan disk yang mengandung aquadest (kontrol negatif). Agar diinkubasi dalam inkubator dengan suhu 37ºC selama 24 jam. Penilaian dilakukan dengan pengamatan dan pengukuran zona inhibisi. Data hasil penelitian membuktikan zona inhibisi yang dihasilkan oleh ekstrak air kopi robusta pada dosis 100% memiliki rata-rata paling tinggi dibandingkan dengan dosis lainnya yaitu 8,13 mm, namun lebih kecil dibandingkan dengan imipenem yang memiliki rata-rata zona inhibisi sebesar 25 mm. Hal ini menunjukkan bahwa ekstrak air kopi robusta memiliki efek antibakteri terhadap Pseudomonas aeruginosa.

Daya Hambat Ekstrak Kopi Robusta terhadap Pertumbuhan Bakteri Streptococcus Pyogenes Sofyan Al Fauzi; Hendro Sudjono Yuwono; Wiwiek Setiowulan
Bandung Conference Series: Medical Science Vol. 2 No. 1 (2022): Bandung Conference Series: Medical Science
Publisher : UNISBA Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29313/bcsms.v2i1.1289

Abstract. Streptococcus pyogenes is a major human pathogen causing a broad spectrum of clinical illnesses from mild ones such as pharyngitis and skin infections, to more severe diseases such as sepsis, streptococcal toxic shock syndrome, post-streptococcal glomerulonephritis, acute rheumatic fever, and rheumatic heart disease. It has reported the failure of ampicillin therapy against S. pyogenes. Therefore, new antibacterial agents with high potency are needed. One of the plants that empirically have antibacterial activity is robusta coffee. Roasted robusta coffee bean extract (Coffea canephora) contains several antibacterial agents. Such as chlorogenic acid, flavonoids, trigonelline, caffeine and hydrogen peroxide (H2O2). The purpose of the study was to determine the comparison of the inhibitory effect of robusta coffee extract and ampicillin in agar media culture of Streptococcus pyogenes. The method used was Kirby-Bauer disc diffusion which consisted of treatment groups with 100%, 50%, 25%, and 12.5% concentrations of robusta coffee extract, a positive control group (ampicillin 10 µg) and a negative control group (aquadest). The results showed that the average value of the largest inhibition zone was found in the positive control of ampicillin which was 38.49 mm (very sensitive), followed by 100% coffee extract was 15.24 mm (sensitive), 50% coffee extract was 11.38 mm (moderately sensitive), and 25% coffee extract was 8.13 mm (moderately sensitive). Robusta coffee extract 12.5% did not form an inhibition zone, similar to aquadest as a negative control. In conclusion, 100%, 50%, 25%, and 12,5% robusta coffee extract has a good inhibitory effect against the growth of Streptococcus pyogenes bacteria but is still lower than ampicillin. Abstrak. Streptococcus pyogenes adalah patogen penting pada manusia sebagai penyebab penyakit dengan spektrum klinis luas, mulai dari yang ringan seperti faringitis dan infeksi kulit hingga yang berat seperti sepsis, sindrom syok toksik streptokokus, glomerulonefritis akut pasca streptokokus, demam rematik akut, dan penyakit jantung rematik. Kegagalan terapi ampisilin terhadap S. pyogenes telah banyak dilaporkan, sehingga diperlukan produk antibakteri baru yang memiliki potensi tinggi. Salah satu tanaman yang secara empiris memiliki aktivitas antibakteri adalah kopi robusta. Ekstrak biji kopi robusta (Coffea canephora) yang telah dipanggang mengandung berbagai zat antibakteri seperti asam klorogenat, flavonoid, trigonelin, kafein, dan hydrogen peroksida (H2O2). Tujuan penelitian adalah membandingkan daya hambat ekstrak kopi robusta dengan ampisilin terhadap kultur bakteri Streptococcus pyogenes. Metode yang digunakan adalah difusi cakram Kirby-Bauer yang terdiri dari kelompok perlakuan ekstrak kopi robusta konsentrasi 100%, 50%, 25%, dan 12,5%, kelompok kontrol positif (ampisilin 10 µg) dan kontrol negatif (akuades). Hasil nilai rerata zona hambat terbesar terdapat pada kontrol positif ampisilin yaitu 38,49 mm (sangat sensitif), kemudian diikuti dengan zona hambat pada ekstrak kopi robusta 100% yaitu 15,24 mm (sensitif), ekstrak kopi robusta 50% yaitu 11,38 mm (cukup sensitif), dan 25% yaitu 8,13 mm (cukup sensitif). Pada ekstrak kopi robusta 12,5% tidak terbentuk zona hambat, setara dengan kontrol negatif. Simpulan: ekstrak kopi robusta 100%, 50%, 25%, dan 12,5% memiliki daya hambat yang baik, namun lebih lemah dibandingkan ampisilin terhadap pertumbuhan bakteri Streptococcus pyogenes.

Daya Hambat Ekstrak Air Kopi Robusta (Coffea Canephora) terhadap Bakteri Propionibacterium Acnes Ilham Mauludin; Hendro Sudjono Yuwono; Dicky Santosa
Bandung Conference Series: Medical Science Vol. 2 No. 1 (2022): Bandung Conference Series: Medical Science
Publisher : UNISBA Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29313/bcsms.v2i1.1926

Abstract. The prevalence of acne vulgaris in Indonesia as much as 80-85% is teenagers peak at 15-18 years. Topical and oral antibiotics suppressed the activity of Propionibacterium acnes in acne. It can cause resistance and result in therapy failure. The purpose was to determine the antibacterial effect of Robusta Coffee (Coffea canephora) aqueous extract. This research is a descriptive experimental study using isolates of bacteria P. acnes ATCC 11827, taken from the Unisba Pharmaceutical Microbiology Lab, with the method of disc diffusion and adapted to the standard 0.5 McFarland. Six research groups were comprised four concentrations of aqueous extract. It is namely 25%, 50%, 75%, and 100%. Positive control (erythromycin antibiotics), and negative control (aqua des) which was then incubated for 24 hours at 37°C and it measured the diameter of the inhibition zone in millimeters. The results showed that the largest inhibition zone at 100% concentration was 7.90 mm. Of 50% and 25% were not detected. The erythromycin inhibition zone formed 23.99 mm. Coffee can overcome bacterial infections that cause acne because it can produce H₂O₂ and create a hyperosmotic solution atmosphere. This study concludes that robusta coffee water extract can inhibit the growth of bacteria P. acne, but the inhibition zone is lower than the erythromycin inhibition zone. Abstrak. Prevalensi akne vulgaris di Indonesia sebanyak 80-85% adalah usia remaja puncaknya pada usia 15-18 tahun. Penggunaan antibiotik untuk menekan aktivitas Propionibacterium acne pada jerawat, dapat menyebabkan resistensi. Tujuan penelitian ini adalah mengetahui daya hambat ekstrak air Kopi Robusta (Coffea canephora) pada media agar kultur Propionibacterium acne. Penelitian ini merupakan studi deskriptif eksperimental in vitro menggunakan isolat bakteri P. acnes, yang diambil dari Lab Mikrobiologi Farmasi Unisba, dengan metode disc diffusion dan disesuaikan dengan standar 0,5 McFarland. Terdapat enam kelompok penelitian terdiri dari empat konsentrasi ekstrak air yaitu 25%, 50%, 75% dan 100%, kontrol positif (antibiotik eritromisin), dan kontrol negatif (cairan akuades) yang selanjutnya diinkubasi selama 24 jam pada suhu 37℃. Zona hambat diukur diameternya dalam milimeter. Hasil penelitian menunjukan zona hambat pada konsentrasi 100% yaitu 7,90 mm, sedangkan pada konsentrasi 50% dan 25% tidak terdeteksi. Pada eritromisin zona hambat terbentuk 23,99 mm. Kopi dapat mengatasi infeksi bakteri penyebab jerawat karena menghasilkan H₂O₂ dan menciptakan larutan hiperosmotik. Kesimpulan ekstrak air kopi robusta dapat menghambat pertumbuhan bakteri P. acne, namun lebih rendah dibandingkan zona hambat eritromisin.

Daya Hambat Ekstrak Air Kopi Robusta (Coffea canephora) terhadap Bakteri Escherichia Coli Luthfiyah Salsabilla; Hendro Sudjono Yuwono; Dhian Indriasari
Bandung Conference Series: Medical Science Vol. 2 No. 2 (2022): Bandung Conference Series: Medical Science
Publisher : UNISBA Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29313/bcsms.v2i1.1869

Abstract. Escherichia coli is a gram-negative rod that lives in the intestine. It is the causative agent of extraintestinal infections. E. coli bacteria are resistant to various antibiotics, notably beta-lactams (24.20%) and gentamicin (58.3-75.0%). Robusta coffee extract mixed in agar forms a hyperosmolar solution and produce hydrogen peroxide (H2O2) which can lyse and kill bacteria. The purpose of this study is to see whether an aqueous extract of Robusta coffee (Coffea canephora) can inhibit the growth of E. coli bacteria. An analysis using Kruskal-Wallis test in IBM SPSS 25. The method used was Kirby-Bauer disc diffusion on Mueller-Hinton Agar. Five samples were a positive control group (gentamicin), a negative control group (aqua des), and a coffee water extract with concentrations of 100%, 75%, and 50%. The agar was incubated in a CO2 incubator at 37ºC for 24 hours. The inhibition zone was then measured. The inhibition zone of 100% extract is 9.36 mm, 75% extract is 8.09 mm, 50% extract 7.42 mm. The positive control had a diameter of 16.47 mm, and the negative control had a diameter of 0 mm. These findings show a statistically significant difference (P<0.05). In conclusion, Robusta coffee water extract can inhibit E. coli bacterial growth, but its inhibitory power is lower than gentamicin. Abstrak. Escherichia coli adalah bakteri usus, gram negatif batang, dan menyebabkan infeksi ekstraintestinal. Bakteri E. coli resisten terhadap beberapa antibiotik terutama beta-laktam (24,20%) dan gentamisin (58,3-75,0%). Ekstrak biji kopi robusta (Coffea canephora) ketika bercampur dengan media agar kultur sel dapat membentuk larutan hyperosmolar dan menghasilkan hidrogen peroksida (H2O2) yang dapat membunuh bakteri. Tujuan penelitian adalah untuk mengetahui aktivitas antibakteri ekstrak air kopi robusta (Coffea canephora) dalam menghambat pertumbuhan bakteri E. coli. Penelitian ini adalah studi analitik eksperimental in vitro dan menggunakan uji Kruskal-Wallis memakai IBM SPSS 25. Metode yang digunakan adalah difusi cakram Kirby-Bauer pada agar Mueller-Hinton dimana dipersiapkan lima kelompok sampel, yakni kelompok kontrol positif (gentamisin), kontrol negatif (akuades) dan kelompok perlakuan ekstak air kopi konsentrasi 100%, 75%, dan 50%, selanjutnya sampel diinkubasi dalam inkubator CO2 dalam suhu 37ºC selama 24 jam. Selanjutnya dilakukan pengukuran zona inhibisi. Hasil penelitian didapatkan rata-rata diameter inhibisi ekstrak air kopi robusta 9,36 mm pada konsentrasi 100%, 8,09 mm pada konsentrasi 75%, dan 7,42 mm pada 50%. Diameter pada gentamisin sebesar 16,47 mm, dan 0 mm pada akuades. Hasil tersebut menunjukan terdapat perbedaan yang bermakna (P<0,05). Kesimpulannya adalah ekstrak air kopi robusta dapat menghambat pertumbuhan bakteri E. coli, namun daya hambatnya lebih rendah dibandingkan gentamisin.

Perbandingan Gambaran Penyembuhan Luka Sayat pada Tikus Wistar (Rattus Novergicus) Menggunakan Bubuk Kopi dengan Bubuk Kafein Muhammad Rifky Dzikrillah; Hendro Sudjono Yuwono; Tryando Bhatara
Bandung Conference Series: Medical Science Vol. 3 No. 1 (2023): Bandung Conference Series: Medical Science
Publisher : UNISBA Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29313/bcsms.v3i1.6462

Abstract. Coffee is a mixture of several complex chemicals that play a role in several bioactivities in coffee. Coffee can be used as a topical wound dressing for acute and chronic wounds, the content of caffeine and chlorogenic acid in coffee have anti-inflammatory and antioxidant effects that can suppress reactive oxygen species which has the effect of accelerating wound healing in the inflammatory phase. The research was conducted in August. This research is an experimental type research conducted in the laboratory with an in vivo preclinical experimental design that compares the appearance of wound healing in Wistar rats (Rattus novergicus) using robusta coffee powder and caffeine powder. The rats used in this study were about 24 rats. In this study, rats were divided into two coffee and caffeine treatment groups and two positive and negative control groups. In one group consists of 6 rats. The study was conducted for 1 week by assessing the description of wound healing through indicators; 1) dryness of the wound, 2) assessment of the wound edges (hyperemic or not), and 3) wound size. Data were obtained by observing the progress of wound healing in rats. Based on the results of statistical tests using one way ANOVA and Kruskall Wallis, the results showed that there was no significant difference in wound healing in the coffee and caffeine groups. This result is likely due to the influence of several factors such as; bandage sticking, stress, temperature, doses exceeding the LD50, excessive vasodilation, and disease in rats during the study period Abstrak. Kopi merupakan campuran beberapa bahan kimia kompleks yang berperan dalam beberapa bioaktivitas dalam kopi. Kopi dapat menjadi topical wound dressing untuk luka akut maupun kronis. Kandungan kafein, dan asam klorogenat dalam kopi memiliki efek anti-inflamasi dan antioksidan yang dapat menekan dari reactive oxygen species yang efeknya mempercepat penyembuhan luka pada fase inflamasi. Penelitian dilaksanakan bulan Agustus. Penelitan merupakan penelitian jenis eksperimental yang dilakukan di laboratorium dengan rancangan eksperimental preklinik in vivo yang membandingkan gambaran penyembuhan luka sayat pada subjek tikus wistar (Rattus novergicus) dengan menggunakan bubuk kopi robusta dan bubuk kafein. Tikus yang digunakan dalam penelitian ini berjumlah 24 ekor tikus. Dalam penelitian tikus terbagi menjadi dua kelompok perlakuan kopi dan kafein serta dua kelompok kontrol positif dan negatif. Dalam satu kelompok terdiri dari 6 tikus. Penelitian dilakukan selama 1 minggu dengan menilai gambaran penyembuhan luka melalui indikator; 1) kekeringan luka, 2) penilaian tepi luka (hiperemis atau tidak), dan 3) ukuran luas luka. Data diperoleh dengan memantau proses penyembuhan luka pada tikus. Berdasarkan hasil uji statistik menggunakan one way anova dan kruskall wallis di dapatkan hasil tidak ada perbedaan yang signifikan pada penyembuhan luka pada kelompok kopi maupun kelompok kafein. Hasil ini kemungkinan dikarenakan pengaruh dari beberapa faktor seperti; perban menempel, stres, suhu, dosis yang melebihi LD50, vasodilatasi yang berlebih, dan penyakit yang menimpa tikus pada masa penelitian

Daya Hambat Ekstrak Air Kopi Robusta (Coffea Canephora) Dibandingkan Ekstrak Air Kopi Instan dengan Gula terhadap Bakteri Staphylococcus Aureus (ATCC© 25923™) Saphiera Damayanti; Hendro Sudjono Yuwono; Listya Hanum Siswanti
Bandung Conference Series: Medical Science Vol. 3 No. 1 (2023): Bandung Conference Series: Medical Science
Publisher : UNISBA Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29313/bcsms.v3i1.6682

Abstract. Staphylococcus aureus is a facultative anaerobic gram-positive bacteria, and has a polysaccharide capsule. Robusta coffee water extract (Coffea canephora) has greater bacteriostatic activity because it contains highly active compounds such as Caffeine and Trigonelin. The antibacterial activity is an alternative treatment for choosing instant coffee with sugar to kill or inhibit antibiotic-resistant bacteria. This study aimed to determine the antibacterial power of Robusta coffee water extract (Coffea canephora) and instant coffee water extract with sugar. This study used experimental research using IBM SPSS 25 software. The method used was Kirby Bauer's disc diffusion with 7 samples, namely Robusta coffee (Coffea canephora) water extract in three concentrations, namely 50%, 75%, and 100%, instant coffee extract with sugar in three concentrations, namely 50%, 75%, and 100% as treatment, and Gentamicin as a positive control. The results showed that the aqueous extract of Robusta coffee (Coffea canephora) had an average zone of inhibition at a concentration of 100% (16.43 mm), a concentration of 75% (15.87 mm), and a concentration of 50% (14.54 mm). At the same time, no zone of inhibition was found in the water extract of instant coffee with sugar at concentrations of 50%, 75%, and 100% for S. aureus. In conclusion, the inhibition power of Robusta coffee water extract is more potent than instant coffee water extract with sugar against Staphylococcus aureus bacteria. Abstrak. Staphylococcus aureus adalah bakteri gram positif fakultatif anaerob, dan memiliki kapsul polisakarida. Ekstrak air kopi Robusta (Coffea canephora) memiliki aktivitas bakteriostatik karena memiliki kandungan senyawa aktif yang tinggi seperti Kafein dan Trigonelin. Aktivitas antibakteri menjadi alternatif pengobatan untuk memilih kopi instan dengan gula untuk membunuh atau menghambat bakteri yang resisten antibiotik. Tujuan penelitian ini adalah mengetahui antibakteri ekstrak air kopi Robusta (Coffea canephora) dan ekstrak air kopi instan dengan gula terhadap bakteri Staphylococcus aureus. Penelitian ini menggunakan studi eksperimental dengan perangkat lunak IBM SPSS 25. Metode yang digunakan dengan disc diffusion Kirby Bauer dengan 7 sampel, yakni ekstrak air kopi Robusta (Coffea canephora) dalam tiga konsentrasi yaitu 50%, 75%, dan 100%, ekstrak kopi instan dengan gula dalam tiga konsentrasi yaitu 50%, 75%, dan 100% sebagai perlakuan, dan antibiotik Gentamisin sebagai kontrol positif. Hasil penelitian didapatkan ekstrak air kopi Robusta (Coffea canephora) memiliki rata-rata zona inhibisi yaitu pada konsentrasi 100%, (16,43 mm), konsentrasi 75% (15,87 mm) dan konsentrasi 50% (14,54 mm). Sedangkan tidak ditemukan zona inhibisi pada ekstrak air kopi instan dengan gula pada konsentrasi 50%, 75% dan 100% terhadap S. aureus. Kesimpulannya, bahwa daya hambat ekstrak air kopi Robusta lebih kuat dibandingkan dengan ekstrak air kopi instan dengan gula terhadap bakteri Staphylococcus aureus.

Co-Authors , Suwarman A. Muthalib Nawawi Abel Tasman Yuza Adria Adnan Aisyah Amanda Hanif Akhmad Fatharoni Andre Akbar Mubarok Daisy Grace Agustina Dhian Indriasari Dicky Santosa Dwika Audiyananda Eddy Hermanto Eka Hendryanny Fadhli Rajif Tangke Galuh Alviana Galuh Alviana, Galuh Ilham Mauludin Ismawati Ismet Muchtar Nur Isnandar Isnandar , Iwan Abdul Rachman Kasman Manullang Kasman Manullang Listya Hanum Siswanti Luthfiyah Salsabilla Mayasari, Ferra Meike Rachmawati MUCHTAN SUJATNO Muchtan Sujatno Muhammad Gibran Al Madani Muhammad Rifky Dzikrillah Muhammad Rifky Dzikrillah Naura Mufida Marsya Naura Mufida Marsya Nova Sylviana Oky Haribudiman Purwaningsih, Sriwahyuniati Reni Farenia Reni Farenia Rifira Hanifah Rizki Perdana Rizky Alifian Ramadhan Saphiera Damayanti Sassty Julya Hidayat Sitanggang, Rully H. Sofyan Al Fauzi Susanti Dharmmika Susanto, Tirta Darmawan Tangke, Fadhli Rajif Tirta Darmawan Susanto Tryando Bhatara Wiwiek Setiowulan

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