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All Journal Jurnal Penelitian Saintek Bioma : Berkala Ilmiah Biologi AL KAUNIYAH Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Pharmaciana: Jurnal Kefarmasian Eksergi: Chemical Engineering Journal Journal of Natural Sciences and Mathematics Research Jurnal Kimia Riset Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Biosaintifika: Journal of Biology & Biology Education Buletin Anatomi dan Fisiologi (Bulletin of Anatomy and Physiology) JKPK (Jurnal Kimia dan Pendidikan Kimia) International Journal of Active Learning Indigenous Biologi Bioed : Jurnal Pendidikan Biologi BIOCHEPHY: Journal of Science Education Jurnal Ilmiah Sains Gunung Djati Conference Series Engagement: Jurnal Pengabdian Kepada Masyarakat Jurnal Pengabdian Kepada Masyarakat
Elizabeth Betty Elok Kristiani
Universitas Kristen Satya Wacana
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Secondary Metabolites and Antioxidant Activity of Ethanolic Extract of Faloak (Sterculia quadrifida) Hory Iramaya Dillak; Elizabeth Betty Elok Kristiani; Sri Kasmiyati
Biosaintifika: Journal of Biology & Biology Education Vol 11, No 3 (2019): December 2019
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v11i3.20736

Abstract

Faloak (Sterculia quadrifida) is a medicinal plant used by the people of East Nusa Tenggara to treat lumbago, liver dysfunction and to restore the stamina. The research aims were to determine the qualitative and quantitative content of flavonoids, phenols, and tannins, as well as to examine the antioxidant activity of roots, stem barks, leaves, fruits and seeds extracts of faloak plant. Each organ was extracted with ethanol 70% using the maceration method. The qualitative content of bioactive compounds was determined using the phytochemical screening method. The determination of bioactive compounds concentration was using spectrophotometric methods and antioxidant activity was using the DPPH method. The result of phytochemical screening showed that all of the extracts were exhibit phenols compounds, but the flavonoids and tannins were only found in roots, barks, leaves, and fruits extracts. The quantitative content of total flavonoids of roots, barks, leaves, fruits, and seeds was 48.09; 62.76; 12.56; 11.91 and 1.55 mg/g, while the phenols total content were 82.90; 45.37; 3.43; 29.50 and 2.89 mg/g. Tannins total content were 71.26; 59.64; 10.52; 13.18 and 14.12 mg/g samples respectively. The stem barks and roots extracts showed a very strong antioxidant activity, while leaves, fruits, and seeds extracts belong to the strong category.  The potential of faloak as an antioxidant has been widely studied, especially in the stem bark. Studies on the antioxidant activity of roots, leaves, fruits, and seeds can provide new information about the benefits of phaloac plants as a source of natural antioxidants.
The Combination of Phyllanthus niruri, Euphorbia hirta, and Loranthus sp. as a Source of Antioxidant Agents Elizabeth Betty Elok Kristiani; Sri Kasmiyati
Biosaintifika: Journal of Biology & Biology Education Vol 13, No 2 (2021): August 2021
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v13i2.30718

Abstract

Meniran (Phyllanthus niruri), patikan kebo (Euphorbia hirta), and benalu (Loranthus sp.) have often been used by people as medicinal plants. This research aimed to measure the levels of flavonoids, phenolics, and ascorbic acid compounds, as well as the Free Radical Scavenging (FRS) activity of ethyl acetate extract from the mixture of P. niruri, E. hirta, and Loranthus sp. The FRS activity was measured with the 2,2-diphenyl-1-picrylhydrazyl (DPPH) method. The level of compounds was measured by using the spectrophotometry method with specific reagents. The result of the FRS activity in ethyl acetate extract from the mixture of P. niruri, E. hirta, and Loranthus sp. varied depending on its dose. The measure of FRS in the P. niruri: E. hirta: Loranthus sp 0:0:1 (K-OOL) composition showed a strong result with a value of IC50 97.2 ± 2.1 ppm, while in the 0:0.5:0.5 (K-OEL) composition it was moderate with a value of IC50 147.6 ± 6.5 ppm. The other compositions showed weak and inactive results. The K-OOL composition had the highest flavonoid and phenolic content that were 298.8±0.00 mg QE/g extract and 141.5±2.85 mg GAE/g extract respectively. The composition with the highest ascorbic acid content (298.8±0.00 mg/g extract) was K-OEO composition. So far, research on medicinal plants is still limited to one type of plant. The combination of several types of plants in several formulations allows obtaining a composition that can produce maximum antioxidant capacity. Therefore, this research is expected to produce a combination formulation of various types of medicinal plants that have the K-OOL composition very strong antioxidant activity and can be used as herbal medicines.
Effect of Induced Polyploidy on Plant Growth, Chlorophyll and Flavonoid Content of Artemisia cina Sri Kasmiyati; Elizabeth Betty Elok Kristiani; Maria Marina Herawati
Biosaintifika: Journal of Biology & Biology Education Vol 12, No 1 (2020): April 2020
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v12i1.22548

Abstract

Artemisia cina is one of a member of genus Artemisia that has potential as a medicinal plant. However, the levels of Artemisia medicinal bioactive compounds are very low. Polyploidization is an alternative method that can enhance of growth and secondary metabolite productions of plants. The aims of this research were to determine the effect of polyploid induction using colchicine and plant growth regulator toward plant growth, the chlorophyll, kaemferol and quercetin contents of A. cina. Four different A. cina used in this research consisted of two diploid genotypes (TWN and KJT) and two polyploid genotypes (J and M). Induction of mutant polyploid was conducted using colchicine and combination of plant growth regulator benziladenyl (BA) and 2,4-dichlorophenoxyacetic acid (2,4-D). The measured plant growth parameters were dry weight, leaf area, and plant height. The chlorophyll content of leaves was determined spectrophotometrically, and flavonoid content determined using HPLC. The result showed that the polyploid genotype (M) significantly decreased leaf and root growth compared to the KJT and TWN. In A. cina plants, polyploidization using colchicine is more effective in increasing the biomass than using combination plant growth regulator BA and 2,4-D. The flavonoid content of KJT was the lowest, and significantly different compared to the other plants. This study provides new information about the effect of polyploid on growth and flavonoid content in A. cina. This can be  useful information to develop A. cina to become a medicinal plant.
Using a Participatory Learning and Action Approach to Improve Young Papuans and Moluccans’ Knowledge of Herbal Drinks to Increase Immunity During the COVID-19 Pandemic Rully Adi Nugroho; Sri Kasmiyati; Elizabeth Betty Elok Kristiani; Vincentia Irene Meitiniarti; Agna Sulis Krave
Engagement: Jurnal Pengabdian Kepada Masyarakat Vol 5 No 2 (2021): November 2021
Publisher : Asosiasi Dosen Pengembang Masyarajat (ADPEMAS) Forum Komunikasi Dosen Peneliti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52166/engagement.v5i2.788

Abstract

The introduction of coronavirus disease (COVID-19) into the human population represents a tremendous medical and economical crisis. The immune system plays a central role in protecting against this novel virus. This activity aimed to empower young generation using participatory learning and action approach to improve their knowledge about medicinal plants that grow in Indonesia and whose anti-inflammatory, antioxidant, immunomodulatory, and antiviral properties. Organoleptic test results showed that Kunyit Asam (consisted of turmeric and ginger) and Tealang (consisted of butterfly pea and lemongrass) were highly favored by panelists. Our results provide valuable information on the medicinal plants used as an immunity booster within the studied community.
Combination of polyherbal Phyllanthus reticulatus with Zingiber officinale and Cymbopogon citratus to optimize the antioxidant capacity Elizabeth Betty Elok Kristiani; Sri Kasmiyati; Yohanes Martono
Pharmaciana Vol 14, No 1 (2024): Pharmaciana
Publisher : Universitas Ahmad Dahlan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.12928/pharmaciana.v14i1.28002

Abstract

Currently, the public is interested in polyherbal-based foods and beverages as a source of natural antioxidants. The aim of the study is to evaluate the antioxidant properties and the phenolic and flavonoid compounds of formulations containing Z. officinale, C. citratus, and P. reticulatus (ZCP). Each sample was extracted using the maceration process in an ethanol solvent at room temperature for three 72-hour periods. There were fourteenth formulation of Z. officinale rhizome, C. citratus leaves, and P. reticulatus fruit which used Design of Expert (DoE). The DPPH method was used to determine the power of antioxidants. The flavonoid content of the extract was measured using the colorimetric method and AlCl3 reagent, while phenolics content using Folin-Ciocalteu. The formulations ZCP 1:0:0, 0:0:1, and 1:1:1 showed the antioxidant capacity in a strong categorization, with an IC50 value less than 50 µg/ml, while ZCP 0:1:0 was in a weak categorization (IC50 > 250 µg/ml). Another ZCP formulation was in a medium category. The ZCP 1:1:1 formulation was suggested as the best one for this investigation, which contains three plant samples. This formulation is interesting for further toxicity studies and in vivo testing so that it can be applied as an antioxidant-rich supplement product.
Peningkatan Laju Pertumbuhan Tagetes erecta L. pada Cekaman Krom Heksavalen dengan Trichoderma viride Rimanti, Audri Septina Putri; Kasmiyati, Sri; Kristiani, Elizabeth Betty Elok
Buletin Anatomi dan Fisiologi Volume 9, Nomor 1, Tahun 2024
Publisher : Departemen Biologi Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/baf.9.1.2024.48-56

Abstract

 Kromium heksavalen (Cr6+) merupakan polutan kromium dengan toksisitas lebih tinggi dibandingkan kromium trivalen. Tagetes erecta merupakan tanaman fitoremediator yang sudah banyak diteliti untuk meremediasi lingkungan yang tercemar polutan Cr. Trichoderma viride adalah satu jenis jamur yang dapat dimanfaatkan sebagai stimulator pertumbuhan tanaman pada lingkungan yang tercemar logam berat. Tujuan dari penelitian ini adalah untuk menganalisis efek T. viride terhadap laju pertumbuhan tanaman T. erecta pada kondisi cekaman logam Cr6+. Penelitian ini dilakukan menggunakan rancangan acak lengkap dua faktor. Faktor pertama adalah dosis kerapatan spora T. viride terdiri dari 0 (tanpa) dan 108 sebanyak 10 ml/tanaman. Faktor kedua adalah konsentrasi Cr6+ terdiri dari 0, 10, 50, dan 100 mg Cr6+/L. Hasil penelitian menunjukkan bahwa T. viride mampu meningkatkan secara nyata laju pertumbuhan tinggi tanaman, jumlah daun dan jumlah bunga T. erecta pada kondisi cekaman Cr6+.  T. viride mampu meningkatkan tinggi tanaman kontrol dan tanaman yang diberi perlakuan Cr6+ 10, 50 dan 100 mg Cr6+/L berturut-turut sebesar 5,49%, 4,52%, 7,80%, dan 9,88%, sedangkan jumlah daun tanaman yang diberi perlakuan T. viride berturut-turut meningkat sebesar 8,01%, 10,17%, 14,33% dan 18,95% dibandingkan tanaman T. erecta tanpa perlakuan penambahan T. viride. Jumlah bunga pada tanaman yang diberi perlakuan T. viride dengan cekaman Cr6+ sebesar 10, 50 dan 100 mg Cr6+/L mengalami peningkatan berturut-turut sebesar 65,38%, 52,63%, 64,29%, dan 70,00% dibandingkan tanaman tanpa perlakuan T. viride. Hexavalent chromium (Cr6+) is a chromium pollutant with higher toxicity than trivalent chromium. Tagetes erecta is a phytoremediator plant that has been widely studied to remediate environments contaminated with Cr pollutants. Trichoderma viride is a species of fungus that can be used as a plant growth stimulator in environments contaminated with heavy metals. The aim of this study was to analyze the effect of T. viride on the growth rate of T. erecta plants under Cr6+ metal stress conditions. This research was conducted using a two-factor completely randomized design. The first factor is the dose of T. viride spore density consisting of 0 (control) and 108 as much as 10 ml/plant. The second factor is the Cr6+ concentration consisting of 0, 10, 50, and 100 mg Cr6+/L. The results showed that T. viride was able to significantly increase the growth rate of plant height, number of leaves and number of flowers of T. erecta under Cr6+ stress conditions. T. viride was able to increase the height of control plants and plants treated with Cr6+ 10, 50 and 100 mg Cr6+/L respectively by 5.49%, 4.52%, 7.80% and 9.88%, while the number leaves of plants treated with T. viride increased by 8.01%, 10.17%, 14.33% and 18.95% respectively compared to T. erecta plants without the addition of T. viride. The number of flowers on plants treated with T. viride with Cr6+ treatments of 10, 50 and 100 mg Cr6+/L increased respectively by 65.38%, 52.63%, 64.29% and 70.00% compared to plants without T. viride treatment.
MENGEMBANGKAN KAMPUS HIJAU: KONDISI PADUK PENGELOLAAN LIMBAH DI UNIVERSITAS KRISTEN SATYA WACANA Nugroho*, Rully A.; Meitiniarti, V Irene.; Krave, Agna S.; ,, Djohan; Sukmana, Andreas Binar Aji.; Cahyaningrum, D C.; Hastuti, S P.; Kristiani, Elizabeth Betty Elok.; Keliat, Natalia R.; Kasmiyati, S; ,, Sucahyo; Rahardja, P M.; Putri, A C.
INDIGENOUS BIOLOGI : JURNAL PENDIDIKAN DAN SAINS BIOLOGI Vol 7 No 2 (2024): INDIGENOUS BIOLOGI : JURNAL PENDIDIKAN DAN SAINS BIOLOGI
Publisher : Prodi Pendidikan Biologi FKIP Universitas Kristen Artha Wacana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33323/indigenous.v7i2.584

Abstract

Saat ini, gerakan kampus hijau dan berkelanjutan mulai menjadi tren baru di berbagai universitas dalam dan luar negeri. Penelitian ini bertujuan untuk mendapatkan gambaran kondisi paduk berdasarkan kriteria limbah (WS) dari UI GreenMetric di Universitas Kristen Satya Wacana (UKSW). Penelitian ini menggunakan kuesioner UI GreenMetric dan wawancara terhadap kepala-kepala direktorat dan laboratorium yang bertanggungjawab terhadap pengelolaan limbah di UKSW, termasuk pihak ketiga yang ditugasi untuk mengelola limbah. Hasil studi menunjukkan bahwa UKSW memiliki program kampus hijau untuk mewujudkan kampus yang ramah lingkungan, UKSW telah mengimplementasikan 3R yang terintegrasi dengan pengelolaan lingkungan kampus dalam rangka mewujudkan lingkungan kampus yang lebih hijau dan berkelanjutan. UKSW direkomendasikan agar ke depan dapat mengimplementasikan strategi-strategi ekonomi sirkuler dalam pengelolaan limbah sehingga dapat mencapai tujuan pengelolaan dan fungsi lingkungan kampus berkelanjutan.
PENDAMPINGAN PENINGKATAN NILAI EKONOMI KOMODITAS GULA TUMBU MENJADI PERMEN BAGI MASYARAKAT KABUPATEN KUDUS Elizabeth Betty Elok Kristiani; Agna Sulis Krave
JURNAL PENGABDIAN KEPADA MASYARAKAT Vol. 1 No. 2 (2021): Jurnal Abdikmas
Publisher : LPPM Universitas Kahuripan Kediri

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51158/abdikmas.v1i2.487

Abstract

Abstrak Gula tumbu, sebagai usaha home industry warga Desa Soco maupun Desa Samirejo, Kecamatan dawe, Kabupaten Kudus, Jawa Tengahmemiliki kendala berupa rendahnya harga jual sehingga muncul kecenderungan modal tidak bekembang tetapi malah habis.Kegiatan ini bertujuan untuk mendampingi masyarakat pengusaha gula tumbu untuk dapat meningkatkan nilai ekonomi gula tumbu menjadi permen. Kegiatan berupa pengayaan teori, praktek bersama pendamping dan praktek mandiri, serta evaluasi pembuatan permen dari gula tumbu. Ada empat kelompok yang terlibat dalam pembinaan ini yaitu dua kelompok beranggotakan ibu rumah tangga, satu kelompok beranggotakan pemudi desa, dan satu kelompok lagi beranggotakan para muda yang berstatus mahasiswa.Masyarakat sangat antusias dalam mengikuti setiap tahapan kegiatan. Melalui praktek secara nyata, masyarakat dapat memahami lebih nyata baik teori, praktek, dan cara mengatasi kendala yang muncul dalam pembuatan permen dari gula tumbu. Kata kunci: Gula tumbu, permen, kabupaten kudus. Abstract Tumbu sugar is a home industry business for residents of Soco and Samirejo Village, Dawe District, Kudus Regency, Central Java. Due to the obstacle of the low selling price, however, their capitals tend to run out instead of developing. This program aims to assist the community of tumbu sugar entrepreneurs to be able to increase the economic value of tumbu sugar by turning it into candy. The activities included weretheoretical enrichment, assisted and independent practice, as well as evaluation of thecandy production from tumbu sugar. Four groups from the village took part in this coaching, namely two groups consisting of housewives, one group consisting of young women, and one group consisting of university students. The community participated in every stage of the activities enthusiastically. Through direct practice, the community could better understand the theories, implementation, and ways to overcome the obstacles faced during the production ofcandy from tumbu sugar. Key words: tumbu sugar, candy, Kudus Regency.
Tinjauan Pustaka: Inovasi Bahan Ajar Ekosistem Melalui Pendekatan Potensi Lokal sebagai Solusi Peningkatan Motivasi dan Hasil Belajar Ie, Johanes Juan Yutama Putra; Kasmiyati, Sri; Meitiniarti, Vincentia Irene; Kristiani, Elizabeth Betty Elok
Bioed : Jurnal Pendidikan Biologi Vol 13, No 1 (2025): Bioed : Jurnal Pendidikan Biologi
Publisher : Universitas Galuh

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25157/jpb.v13i1.18204

Abstract

Inovasi bahan ajar berbasis potensi lokal di materi ekosistem merupakan salah satu bentuk upaya guru-guru untuk meningkatkan motivasi dan hasil belajar peserta didik kelas X. Pengembangan bahan ajar merupakan topik yang banyak dikembangkan, namun untuk pemanfaatan potensi lokal belum banyak dilakukan. Maka, peneliti mengkaji artikel-artikel penelitian terkait pengembangan atau inovasi bahan ajar berbasis potensi lokal pada materi ekosistem pada peserta didik kelas X dengan tujuan penelitian untuk mengetahui bentuk-bentuk pengembangan bahan ajar berbasis potensi lokal dan pengaruh penggunaan bahan ajar berbasis potensi lokal tersebut terhadap hasil dan motivasi belajar peserta didik kelas X di materi ekosistem. Penelitian ini menggunakan metode literature review terdiri dari lima tahapan. Artikel yang didapatkan ditabulasikan dalam tabel dengan poin yang mengkaji model pengembangan bahan ajar yang digunakan, dasar dilakukan pengembangan bahan ajar, jenis bahan ajar yang dikembangkan dan jenis potensi lokal yang dimanfaatkan, dan pengaruhnya terhadap hasil dan motivasi belajar peserta didik. Kajian artikel-artikel tersebut menjelaskan sebuah kebutuhan untuk mengembangkan bahan ajar berbasis potensi lokal di materi ekosistem untuk meningkatkan pemahaman, hasil dan motivasi belajar pada peserta didik kelas X.  Faktor penyebab dari bahan ajar dapat mempengaruhi hasil dan motivasi belajar dikarenakan adanya potensi lokal sehingga peserta didik mudah memahami dan desain bahan ajar yang menarik.
The phytochemical and antibacterial activity of ethanolic extract of Laportea decumana and Laportea aestuans Siahaya, Esty Vicariat; Kristiani, Elizabeth Betty Elok; Kasmiyati, Sri
Pharmaciana Vol. 15 No. 1 (2025): Pharmaciana
Publisher : Universitas Ahmad Dahlan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.12928/pharmaciana.v15i1.30099

Abstract

Plants have been an important medicinal resource for centuries, offering a rich source of bioactive compounds used in traditional and modern medicine. This study aims to analyze the comparison between Laportea decumana and Laportea aestuans, focusing on the two main aspects, phytochemical screening and antibacterial activity. The extraction of the maceration process uses ethanol 96% as the solvent at room temperature for 72 hours. The qualitative phytochemical analysis uses HCl and FeCl3 0.1% reagents to determine flavonoids and phenolic compounds, respectively. The quantitative analysis of total flavonoid content is measured using colorimetric AlCl₃ method, while the determination of phenolic compounds is performed by the Folin-Ciocalteu. The diffusion method is used to determine the antibacterial activity. The 96% ethanol extract of L. decumana leaves has higher flavonoids (69.4 mg GAE/g extract) than phenolics (55.1 mg QE/g extract). However, the L. aestuans has higher phenolic content (55.3 mg QE/g extract) than flavonoids (35.3 mg GAE/g extract). The antibacterial test using agar diffusion method shows that both extracts inhibited the growth of pure strains of E. coli ATCC and S. aureus ATCC, with different potencies. The L. decumana extract showed 1.5 times stronger activity than the L. aestuans extract on the growth inhibitory activity against E. coli bacteria. The results show that the relationship between the antibacterial activity and the phytochemical profile against E. coli is strong but weak against S. aureus.
Co-Authors ,, Djohan Agna Sulis Krave Agung Rimayanto Gintu Agung Rimayanto Gintu Agung Rimayanto Gintu Alberthnico, Ferdian Andreas Binar Aji Sukmana Anggara Mahardika Araujo, Otilda Fatima Ari Hasna Widyapuspa Cahyaningrum, D C. Cahyaningrum, Desti Christian Djurubasa, Dani Karel Marthin Egarani, Grahita Rezhi Grahita Rezhi Egarani Hartati Sulastri Hastuti, S P. Ie, Johanes Juan Yutama Putra Indrianasari, Putri Jeanelsin A.P Langgori Kasmiyati, S Keliat, Natalia Rosa Lilik Linawati Maria Marina Herawati Nabila, Nabila Natalia Rosa Keliat Priyayi, Desi Fajar Purwoko, Agus Putra Ie, Johanes Juan Yutama Putri, A C. Rahardja, P M. Ramagita, Marsahanda Astri Rimanti, Audri Septina Putri Rully Adi Nugroho Setiyarini, Chatarina Titik Siahaya, Esty Vicariat Sri Kasmiyati Sri Yulianto Sucahyo Sucahyo, S Trisianna, Jessica Ayu Trisianna Utomo, Daniel Setyo Vincentia Irene Meitiniarti Wardani, Yulia Kusma Wicaksono, Rifky Adi Yayi Suryo Prabandari Yohanes Martono Yusta, G. Maria