Article Per Year (5 Year)
p-Index From 2021 - 2026
1.704
P-Index
This Author published in this journals
All Journal Jurnal Bios Logos JURNAL ILMU PERTANIAN AGRIVITA, Journal of Agricultural Science BERITA BIOLOGI JURNAL AGROHITA Seminar Nasional Lahan Suboptimal Agroprimatech AGRINULA: Jurnal Agroteknologi dan Perkebunan Pelita Kota Journal Agroland: Jurnal Ilmu-ilmu Pertanian
Rama Riana Sitinjak
Universitas Prima Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Engineering Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Medicine & Pharmacology Social Sciences Transportation Other

Published : 22 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 4 Documents
Search
Journal : BERITA BIOLOGI

 1 
PENGARUH 2,4-D DAN BA TERHADAPINDUKSIKALUS EMBRIOGENIK PADA KULTUR MERISTEM JAHE {Zingiber officinale Rose.) Sitinjak, Rama Riana; Rostiana, Otih; Karyono, Karyono; Supriatun, Titin
BERITA BIOLOGI Vol 8, No 2 (2006)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v8i2.2037

Abstract

The aim of this study was to evaluate the effects of 2,4-dichlorophenoxyacetic acid (2,4-D) and N -benzyladenin (BA) to embryogenic callus induction of meristem culture in ginger {Zingiber officinale Rose). Completely Randomixed Design was applied using factorial pattern, replicated 3 times. Two factors were assigned i.e: 2,4-D (1.0, 2.0 and 3.0 mg/L) and BA (0.0, 1.0, 3.0 and 5.0 mg/L). Result showed that 2,4-D and BA significantly affected the embryogenic callus induction. The highest embryogenic callus (93.33%) was achieved on MS (Murashige & Skoog) containing combination of 1.0 mg/L 2,4-D and 3.0 mg/L BA, after 8weeks incubation.Histological evaluation informed that the incubation of embryogenic calli started from cortex cells adjacent the meristem vascular tissues.
PENGARUH PEMBERIAN EKSTRAK Saccharomyces cerevisiae Hansen TERHADAP KANDUNGAN GOSIPOL PADA KULTUR KALUS Gossypium hirsutum L. Sitinjak, Rama Riana; Siregar, Arbayah H; RE, Rizkita
BERITA BIOLOGI Vol 5, No 2 (2000)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (367.19 KB) | DOI: 10.14203/beritabiologi.v5i2.1133

Abstract

This study concentrated on the effect of Saccharomyces cerevisiae Hansen extract on gosipol content of Gossypium hirsutum L callus cultures. There were many ways commonly used to obtain high secondary metabolites product by using tissue culture,i.e. elicitation.The objective of this study was to increase the production ofgossypol on G. hirsutum callus cultures using elicitor derived from S. cerevisiae.The callus grew optimally and produced gossypol on Linsmaierand Skoog (LS) medium with the addition of 10 M NAA and 10 M 2,4-D. Callus derived from cotyledon was subcultured five times.Callus was then elicited with elicitor derived from autoclaved S. cerevisiae and concentration of elicitor tested were 0.5, 1.0 and 2.5% (b/v). The harvesting times were 0, 2, 4, dan 6 days after elicitation. The gossypol was analyzed qualitatively and quantitatively by using High Performance Liquid Chromatography (HPLC).Gossypol content was influenced significantly by concentration of elicitor and harvesting time.The maximum ofgossypol content was obtained on the 4 day after elicitation i.e. 469.233 ± 2.332 pg/g dry weight in the elicited callus of G. hirsutum.
PENGARUH 2,4-D DAN BA TERHADAPINDUKSIKALUS EMBRIOGENIK PADA KULTUR MERISTEM JAHE {Zingiber officinale Rose.) Rama Riana Sitinjak; Otih Rostiana; Karyono Karyono; Titin Supriatun
BERITA BIOLOGI Vol 8, No 2 (2006)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v8i2.2037

Abstract

The aim of this study was to evaluate the effects of 2,4-dichlorophenoxyacetic acid (2,4-D) and N -benzyladenin (BA) to embryogenic callus induction of meristem culture in ginger {Zingiber officinale Rose). Completely Randomixed Design was applied using factorial pattern, replicated 3 times. Two factors were assigned i.e: 2,4-D (1.0, 2.0 and 3.0 mg/L) and BA (0.0, 1.0, 3.0 and 5.0 mg/L). Result showed that 2,4-D and BA significantly affected the embryogenic callus induction. The highest embryogenic callus (93.33%) was achieved on MS (Murashige & Skoog) containing combination of 1.0 mg/L 2,4-D and 3.0 mg/L BA, after 8weeks incubation.Histological evaluation informed that the incubation of embryogenic calli started from cortex cells adjacent the meristem vascular tissues.
PENGARUH PEMBERIAN EKSTRAK Saccharomyces cerevisiae Hansen TERHADAP KANDUNGAN GOSIPOL PADA KULTUR KALUS Gossypium hirsutum L. Rama Riana Sitinjak; Arbayah H Siregar; Rizkita RE
BERITA BIOLOGI Vol 5, No 2 (2000)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v5i2.1133

Abstract

This study concentrated on the effect of Saccharomyces cerevisiae Hansen extract on gosipol content of Gossypium hirsutum L callus cultures. There were many ways commonly used to obtain high secondary metabolites product by using tissue culture,i.e. elicitation.The objective of this study was to increase the production ofgossypol on G. hirsutum callus cultures using elicitor derived from S. cerevisiae.The callus grew optimally and produced gossypol on Linsmaierand Skoog (LS) medium with the addition of 10 M NAA and 10 M 2,4-D. Callus derived from cotyledon was subcultured five times.Callus was then elicited with elicitor derived from autoclaved S. cerevisiae and concentration of elicitor tested were 0.5, 1.0 and 2.5% (b/v). The harvesting times were 0, 2, 4, dan 6 days after elicitation. The gossypol was analyzed qualitatively and quantitatively by using High Performance Liquid Chromatography (HPLC).Gossypol content was influenced significantly by concentration of elicitor and harvesting time.The maximum ofgossypol content was obtained on the 4 day after elicitation i.e. 469.233 ± 2.332 pg/g dry weight in the elicited callus of G. hirsutum.
Co-Authors Abednego S. Karosekali afrianti, Suratni Agustina, Nur Ariyanti Arbayah H Siregar Arbayah H Siregar, Arbayah H Bayu Pratomo Bayu Pratomo Br Ginting, Friska Andriani Calvin Davidson Silalahi DINGSE PANDIANGAN Dingse Pandiangan Dini Ariyska Edy Fachrial Enda Sabda Gentri Sembiring Ginting, Chelvin Van Roy Harmileni Indra Setiawan Rambe Irni, J Irni, Julaili Karo Sekali, Abednego Suranta Karyono Karyono Karyono Karyono Kurniawan, I Laura J. Pinem Nur Ariyani Agustina Otih Rostiana Otih Rostiana Pinem, Laura Juita Pratomo, B Pratomo, Bayu Purba, Jhon Kevin Rizkita RE Rizkita RE, Rizkita Sihaloho, Martha Adiwaty Siregar, Dendi Alfiansyah Supriyadi Tanjung, Agung Rafiul Naldi Tengku Muhammad Sahiril Anhar Titin Supriatun Titin Supriatun Yustina Sri Sulastri