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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences El-Hayah : Jurnal Biologi Jurnal Produksi Tanaman Journal of Degraded and Mining Lands Management AGRIVITA, Journal of Agricultural Science Folium : Jurnal Ilmu Pertanian Berkala Penelitian Hayati AGRONISMA
Novi Arfarita
Universitas Islam Malang
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Environmental Science Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Other

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Effect of VP3 Biological Fertilizer Enriched with Trichoderma viride FRP3 on Growth and Chlorophyll Content in Soybean Leaves (Glycine max. L) Wandi Wandi; Indiyah Murwani; Novi Arfarita
AGRONISMA Vol. 10 No. 2 (2022)
Publisher : AGRONISMA

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Abstract

Biofertilizer is a living microorganism material that is useful for increasing soil fertility and the quality of a plant's production. VP3 biofertilizer is a liquid biofertilizer formulation made from vermiwash as a carrier, molasses, PEG, and 3 functional bacterial isolates. In this study, the VP3 biofertilizer will be enriched with Trichoderma viride FRP3. This study aims to determine the effect of giving VP3 biofertilizer enriched with Trichoderma viride FRP3 on yield and chlorophyll content in Soybean (Glycine max) leaves. This study was an experiment in polybags conducted at the Greenhouse using a Randomized Block Design consisting of 7 treatments, each treatment repeated 3 times and each replication using 4 plant samples. The variables observed included growth variables consisting of plant height, number of leaves and leaf area and leaf chlorophyll content. Data from observations on each plant parameter were then tested using analysis of variance F test with a level 5% significant, if there is a significant effect between treatments followed by Duncan's test at 5% level.The results showed that the application of VP3 biofertilizer enriched with Trichoderma viride FRP3 had no significant effect on plant growth parameters, because in general the treatment of N (m (using NPK fertilizer) showed high yields compared to other treatments, but the application of VP3 biological fertilizer enriched with Trichoderma viride FRP3 in the chlorophyll content of soybean leaves was able to compensate for the application of inorganic NPK fertilizer.
Pengaruh Suhu Pengeringan pada Viabilitas Agen Hayati Pelet BioferNA Berbahan Dasar Limbah Rumput Laut dan Pengaruhnya pada 2 Bibit Tanaman sekar putri lisnanwati; Mahayu Woro Lestari; Novi Arfarita
AGRONISMA Vol. 10 No. 2 (2022)
Publisher : AGRONISMA

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Abstract

The seaweed industry's solid waste has the potential to be converted into organic fertilizer. The biological fertilizer used in this study was VP3 biological fertilizer, which is a liquid formulation fertilizer made from 3 functional bacterial isolates, molasses, and PEG 1% and vermiwash as a carrier. The goal of this study was to determine the ideal drying temperature for producing BioferNA pellet fertilizer made from waste seaweed. To determine their impact on plant growth, the drying results with the best total viability of biological agents will be tested on tomato and chili plant seeds. The design used in the Biological Agent Viability Test was Completely Randomized Design (CRD) with 10 treatments and was repeated 3 times. The design used in the test on plant seeds used a  Completely Randomized Block Design (RCBD) which consisted of 5 treatments, and was repeated 4 times. All the observed data are analyzed statistically using analysis of variance (ANOVA) 5% significance level, if there is a significant difference betweem the treatments, it will have a further test using different test average with BNT at 5% significance level. The results showed that the drying temperature of BioferNA pellets made from seaweed waste had a significant effect on the total viability of biological agents. The drying temperature of 40°C has a higher average total viability of biological agents than the temperature of 50°C. BioferNA pellets made from seaweed waste, which were added with VP3 and Trichoderma viride FRP3, had a significant effect on seedling height and wet weight of chili and tomato seedlings, but had no significant effect on the root length of tomato seedlings. The observed growth parameters did not significantly affect the V1 (VP3) and V4 (VP3 + T. viride FRP3 + Vacuum) treatments.Keywords : VP3 Biofertilizer, Trichoderma viride FRP3, Seaweed Waste, Tomato, Chili
Efek Pemberian Pupuk Hayati VP3 yang Diperkaya Trichoderma viride FRP3 terhadap Pertumbuhan Fase Generatif Tanaman Kedelai (Glycine max L.) erwin erwinn; Anis Rosyidah; Novi Arfarita
AGRONISMA Vol. 10 No. 2 (2022)
Publisher : AGRONISMA

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Abstract

VP3 biofertilizer is a fertilizer composed of vermiwash, molasses and 1% PEG, with 3 bacterial isolates added. Trichoderma viride FRP3 is a bioremediation agent that can degrade land contaminated with the herbicide glyphosphate. The purpose of this study was to determine the effect of the application of VP3 biofertilizer enriched with Trichoderma viride FRP3 on the growth of the generative phase of soybean (Glycine max L.) grown in greenhouses. The research was conducted in the greenhouse of the Faculty of Agriculture, and Laboratory of Microbiology, University of Islam Malang from November 2021 to February 2022. The research design used a Simple Randomized Block Design (RBD) with 7 treatments and was repeated 3 times. The results of the study showed that treatment N (NPK fertilizer) gave the highest average yield on observations of plant height, number of leaves, leaf area, total flowers, and total pods. The best result on the parameter of the percentage of flowers into pods was in treatment V2 (compost fertilizer + manure + VP3 biofertilizer 3 times application) which was 31,74%. Keywords : VP3 Biofertilizer, Trichoderma viride FRP3, Soybean.
Efektivitas Aplikasi Beberapa Macam Pupuk Organik Dibandingkan Dengan Pupuk Anorganik Terhadap Pertumbuhan dan Kandungan Klorofil Tanaman Kailan (Brassica oleraceae var. alboglabra) adinda widya rahmawati; Nurhidayati Nurhidayati; Novi Arfarita
AGRONISMA Vol. 11 No. 1 (2022)
Publisher : AGRONISMA

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Abstract

Kailan cultivation is generally done by farmers using inorganic fertilizers. This kind of farming system in the long term is detrimental to farmers because the soil will become infertile, the quantity and quality of vegetables will decrease. This study aims to compare the effect of using several kinds of organic fertilizers compared to inorganic fertilizers on the growth of kailan plants. This study used a Simple Randomized Block Design (RBD). There were 7 kinds of treatments, including: P0 = No fertilizer application, P1 = NPK fertilizer 4.5 g/pot, P2 = Compost fertilizer 218 g/pot, P3 = Cow dung fertilizer 397 g/pot, P4 = Chicken manure fertilizer 111 g /pot, P5 = Vermicompost fertilizer 511 g/pot, and P6 = Vermicompost powder fertilizer 147 g/pot. The dose of organic fertilizer applied is equivalent to the N content in NPK fertilizer and converted to the water content of the organic fertilizer. The variables observed included: plant height, number of leaves, leaf area and chlorophyll content. The data collected were analyzed for variance (F test) with a level of 5% to determine the effect of treatment. If the results of the F test have a significant effect, then continue with the further LSD test with a level of 5% to determine the effect of growth on plant yields. The results of this study indicate that at the end of the observation of plant growth (plant height and leaf area) the best was found in the vermicompost treatment, while the number of leaves of inorganic fertilizer, compost, vermicompost and vermicompost powder gave the same number of leaves. However, when viewed from the level of green leaf color (chlorophyll content) as measured by using a SPAD meter, vermicompost powder gave the highest chlorophyll content. Keywords : Kailan, Fertilizer, Growth response
Utilization of Various Organic Wastes as Liquid Biofertilizer Carrier Agents towards Viability of Bacteria and Green Bean Growth Novi Arfarita; Tsuyoshi Imai; Cahyo Prayogo
Journal of Tropical Life Science Vol. 12 No. 1 (2022)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.12.01.01

Abstract

The problem in the production of biofertilizers is that raw materials are cheap, easy to get and apply. Another problem is determining the viability of the consortium microbes in a biofertilizer formulation. This study aims to determine the bacterial viability of various liquid media originating from organic waste as a liquid biofertilizer carrier. Three indigenous bacterial strains under consortium for phosphate soluble (Pantoea ananatis strain 53 (BC32)), non-symbiostic Nitrogen fixation (Bacillus licheniformis strain S45) and stabilizing soil aggregate (Pseudomonas plecoglossidastrain PR19) were added to liquid biofertilizer. The study evaluated 10 treatments using a randomized design with three replicates. The treatments are as follows: Peptone,molasses, Compost wash from seaweed waste, Vermiwash, molasses + glycerol, compost wash from seaweed waste + glycerol, vermiwash + glycerol, molasses + PEG (PolyEthylene Glycols)1%, compost wash seaweed waste + PEG 1%, and Vermiwash + PEG 1%. This biofertilizer formulation (liquid) was kept for 16 weeks at optimumpH 5.5. VP3 (Vermiwash made from vermicompost + PEG 1%) treatment showed thebest viability of bacterial strains during the 16-week storage period. The pathogenicitytest using green bean seeds Vima-1 showed that all liquid formulations of biological fertilizers with the three consortium bacterial isolates did not show signs of diseases and demonstrated better growth than the control treatment. Compared to other treatments, the best growth of bacterial strains was detected with MP2 (Molasses + glycerol) treatment. Formulations using vermiwash and PEG appear to maintain bacterial viability in the formulation effectively. However, the formulation of molasses and glycerol exerts a stimulating effect on sprouts growth.
Uji Pengaruh Pelet Pupuk Hayati VP3 dengan Tambahan Cangkang Telur terhadap Viabilitas Bakteri Fungsional dan Bibit Tanaman Tomat (Solanum lycopersicum L.) Amanda Reha Zahran; Novi Arfarita; Mahayu Woro Lestari
Folium : Jurnal Ilmu Pertanian Vol. 7 No. 2 (2023): Folium : Jurnal Ilmu Pertanian
Publisher : Universitas Islam Malang

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Abstract

This research was conducted to determine the effect of different drying temperatures on the total viability of VP3 bio-pellet fertilizer bacteria and to determine the effect of eggshell composition and VP3 bio-fertilizer formulation in pellets on the growth of tomato plant seedlings. This research was conducted at the Central Laboratory and Halal Center, Islamic University of Malang from June until September 2022. This study used a Completely Randomized Design (RAL) on the viability test of VP3 biological fertilizer pellet bacteria with a total of 12 treatments and repeated 3 times and Randomized Group Design (RAK). ) In the tomato plant seed test consisted of 4 treatments and was repeated 5 times. Observational data were analyzed using the 5% level F test (ANOVA). If there is a significant effect, a further test is carried out with a BNT level of 5%. The results of the bacterial viability test showed that the V3T1 treatment (15% Eggshell Waste + VP3 with a drying temperature of 40oC) had an average total bacterial viability that was not significantly different from the V1T1 treatment (5% Eggshell Waste + VP3 with a drying temperature of 40oC) and V2T1 (10% Egg Shell Waste + VP3 with drying temperature 40oC). The results of the tomato plant seed test showed that the V3T1 treatment (15% Egg Shell Waste + VP3 with a drying temperature of 40oC) had a significant effect on the height of the tomato seedlings and the fresh weight of the tomato seedlings while the best root length of the tomato seedlings was in the V2T1 treatment (Waste Shells). Egg 10% + VP3 with drying temperature 40oC). Keywords: VP3 bio-pellet fertilizer, bacterial viability, tomato seed
The Effect of VP3 Biofertilizer and Compost Application on Red Spinach (Amaranthus dubius) and Green Spinach (Amaranthus viridis) Yield and Quality: The Effect of VP3 Biofertilizer and Compost Application on Red Spinach (Amaranthus dubius) and Green Spinach (Amaranthus viridis) Yield and Quality Arfarita, Novi; Masyhuri, Masyhuri; Sugiarto , Sugiarto; Higuchi, Takaya
Journal of Tropical Life Science Vol. 14 No. 1 (2024)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.14.01.04

Abstract

VP3 biofertilizer is a biological agent that has been formulated in previous research. It was applied once with compost and has been tested on red spinach and green spinach in the greenhouse to see its effect on yield and quality (chlorophyll content, anthocyanins, and shelf life at room temperature). Red spinach and green spinach had shown a significantly different growth response to applying biological fertilizers. VP3 biofertilizer with compost had the highest yield effect but was not significantly different from the recommended Nitrogen Phosphate Potassium (NPK) and Effective Microorganism4(EM4) treatment with compost. The application of VP3 Biofertilizer with NPK fertilizer showed a darker color (higher chlorophyll and anthocyanin content) than the application of biological fertilizers, but the values ​​were not significantly different. The application of VP3 biofertilizer with compost affected the shelf life of both red spinach and green spinach. Spinach plants remained fresh at room temperature for three days of storage, while the other treatments lost freshness on the 3rd day after harvesting and even loosened the leaf on the 4th day after harvesting. Therefore, VP3 biofertilizer has potential to increase the yield and quality of spinach and other vegetable crops. The addition of the application period of VP3 biofertilizer needs to be carried out and observed to increase the yield and quality of plants further. Canonical Variate Analyses (CVA) can distinguish the grouping of treatments based on the selected parameter. Biplot assessment provides information on the strong relationship between the yield of green spinach and red spinach and a number of leaves, leaf area, and plant height. The application of VP3 biofertilizer with compost had a significant effect on the yield and quality and produced different effects between treatments, both in the case of green spinach and red spinach.
Structure and Composition of Major Arbuscular Mycorrhiza (MA) under Different Farmer Management of Coffee and Pine Agroforestry System Prayogo, Cahyo; Prastyaji, Dimas; Prasetya, Budi; Arfarita, Novi
AGRIVITA Journal of Agricultural Science Vol 43, No 1 (2021)
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v1i1.2639

Abstract

Utilization of Arbuscular Mycorrhiza (MA) as beneficial soil microbes is expected to support nutrient demand for improving crop performance. However, under the agroforestry system that facing a problem on light, water, and nutrients competitions, the role of MA is becoming unclear. The purpose of this research was to examine how far different management in Coffee Pines Agroforestry System (CPAS) affects MA structure and compositions. The relationship between soil parameters (e.g. pH, soil organic C) and MA activities was also being evaluated. The selected plot according to existing management practices were chosen as follows: (1) Low management (LC); (2) High management which then compared to (3) Business As Usual (BAU) plot in which were repeated in triplicate. ANOVA and multivariate analysis were employed to determine the effect of the treatments. The result showed that there were significant differences (p<0.05) in the structure and composition of the MA, in terms of the total number of MA spores and the abundance of Glomus sp. under the coffee tree sampling point, while the lowest number was detected in Gigaspora sp. genera. The more intensive land management resulted in a higher abundance of MA biomass which then leads to increased soil P and uptake-P along with MA infection.
Utilization of Vermiwash for the Production of Liquid Biofertilizers and Its Effect on Viability of Inoculant Bacteria and Green Bean Germination Arfarita, Novi; Lestari, Mahayu Woro; Prayogo, Cahyo
AGRIVITA Journal of Agricultural Science Vol 42, No 1 (2020)
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v42i1.2263

Abstract

Contribution of biofertilizer in Indonesia as an alternative material for improving soil fertility and crop production is still limited. This may due to several major obstacles on its utilization and effectiveness regarding to it quality in term of its microbial viability of inoculants on formulations. This study aims to determine the effect of liquid biofertilizer formulations made from vermiwash carrier material on indigenous bacterial viability and its testing on germination of green beans ‘Vima-1’. The study included: (1) optimization of pH for the growth of inoculants bacterial consortium in vermiwash formulations with various additives, (2) viability tests and (3) pathogenicity tests of biological fertilizers on green bean sprouts (vegetative phase). The results showed that the best pH for the growth of the consortium of three bacterial isolates was at 5.5. Liquid formulation with a carrier material in the form of vermiwash plus 1% PEG (VP3 treatment) supported the highest viability of the bacterial consortium during 8 weeks incubation. All treatments did not inhibit the growth of green bean sprouts and symptom of pathogenicity was absent in all treated plants. From all treatments, the tallest green bean sprout was detected at VP3 treatment.
Uji Pengaruh Pelet Pupuk Hayati VP3 dengan Tambahan Cangkang Telur terhadap Viabilitas Bakteri Fungsional dan BibitTanaman Cabai (Capsicum frutescens L.) Shinta Dwi Cahya; Sugiarto, Sugiarto; Arfarita, Novi
Produksi Tanaman Vol. 11 No. 3 (2023): Maret
Publisher : Jurusan Budidaya Pertanian Fakultas Pertanian Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.protan.2023.011.03.06

Abstract

Penelitian dilakukan untuk mengetahui pengaruh suhu pengeringan yang berbeda terhadap total viabilitas bakteri pelet pupuk hayati, dan pengaruh pupuk hayati VP3 dengan komposisi cangkang telur ayam terhadap pertumbuhan bibit tanaman cabai rawit (Capsicum frutescens L). Penelitian dilaksanakan pada bulan Juni – September 2022 di Laboratorium Mikrobiologi, Laboratorium Pusat dan Halal Center Universitas Islam Malang. Metode yang digunakan dalam penelitian ini adalah Rancangan Acak Lengkap (RAL) untuk uji viabilitas agen hayati, terdapat 12 perlakuan dan diulang sebanyak 3 kali. Sedangkan Rancangan Acak Kelompok (RAK) untuk uji pada bibit tanaman cabai, terdiri dari 4 perlakuan dan masing-masing diulang sebanyak 5 kali. Data yang diperoleh dianalisis menggunakan uji F taraf 5% (ANOVA). Apabila terdapat pengaruh nyata dilakukan uji lanjut dengan BNT taraf 5%. Hasil penelitian menunjukkan bahwa Perlakuan V3T1 (VP3 + Cangkang telur 15%, suhu pengeringan 40oC) menunjukan hasil rata-rata tertinggi yang jumlah populasi bakterinya sebesar 86,7 x 109 CFU/g, tetapi perlakuan V3T1 tidak berbeda nyata dengan perlakuan V2T1 (VP3+ Cangkang telur 10%, suhu pengeringan 40oC) dan perlakuan V1T1 (VP3+Cangkang telur 5%, suhu pengeringan 40oC). Pada uji bibit tanaman cabai menunjukkan bahwa perlakuan V3T1 (Limbah Cangkang Telur 15% + VP3 dengan suhu pengeringan 40oC) memiliki pengaruh yang nyata pada tinggi bibit cabai dan bobot segar bibit cabai sedangkan pada panjang akar bibit cabai terbaik terdapat pada perlakuan V2T1 (Limbah Cangkang Telur 10% + VP3 dengan suhu pengeringan 40oC). Kata Kunci : Bibit cabai, Pelet pupuk hayati, Viabilitas bakteri.
Co-Authors adinda widya rahmawati Agus Sugianto Amanda Reha Zahran Anik Malikah Anis Rosyidah Anis Rosyidah Anton Muhibuddin aprilia dwi nastiti ayu azhariah Ayu Nur Setianingsih baltar ardyah pramesti Bambang Siswanto Bambang Siswanto Budi Prasetya Cahyo Prayogo, Cahyo Dhurrotul Roaidha Djuhari - Djuhari Djuhari Djuhari, Djuhari dwi nawang wulan eddrin wahyu febbiliani Ekachai Chukeatirote Endand Safitri, Endand erwin erwinn Feby Syaharani firman aprilianto dwi setiawan Firman Hidayat Hardadi Airlangga Hermawan Susanto hosinatul asror Indiyah Murwani Indiyah Murwani Indiyah Muwarni Indiyah Muwarni Khodiroh Shokibatun Mahayu Woro Lestari Maria Ulfah Mas'ut Khakim, Mohammat Masyhuri Masyhuri Miftahur Rohmah Mohammad Jasa Afroni nurhadiyanti rochmana Nurhidayati, Nurhidayati Nurlailah Nurlailah Prastyaji, Dimas Putri Nur Azizah Qur'ania, Anita rosita dwi S Sugiarto S Sunawan Sari, Sekar Arum Dwi Kartika sekar putri lisnanwati Shinta Dwi Cahya SITI MUSLIKAH Sugiarto , Sugiarto Sugiarto S Sugiarto Sugiarto Sunawan Sunawan Syafarotin Syafarotin Takaya Higuchi Takaya Higuchi, Takaya Tsuyoshi Imai Tsuyoshi Imai Tsuyoshi Imai Tsuyoshi Imai, Tsuyoshi wandi wandi Wiwit Nur Hidayah Yulia Nuraini Yuliana Yuliana